Labshake search
Citations for Zymo Research :
1 - 50 of 120 citations for NT 3 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... to block the 3’-ends from ligation and were then size-selected to 200 ≥ nt (DNA Clean & Concentrator™-5, Zymo Research). 5’ adaptors were attached using T4 RNA ligase (Ambion ...
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bioRxiv - Plant Biology 2021Quote: ... RNA clean-up and separation into small (17-200 nt) and large (>200 nt) RNA fractions was performed using RNA Clean & Concentrator columns (Zymo Research). The large RNA fraction was fragmented according to the protocol by Pfeifer-Sancar et al ...
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bioRxiv - Plant Biology 2019Quote: ... RNA greater than 17 nt was purified first (Zymo Research R1017), and then RNA smaller than 200 nt was enriched (Zymo Research R1015) ...
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bioRxiv - Microbiology 2022Quote: ... The size of 150-400 nt was cut and recovered by Zymo DNA agarose gel extraction kit (Zymo Research) ...
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bioRxiv - Plant Biology 2019Quote: ... and then RNA smaller than 200 nt was enriched (Zymo Research R1015). Next ...
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bioRxiv - Genomics 2022Quote: ... The ligated products (≥200 nt) were then purified by RNA Clean & Concentrator (Zymo Research, R1016). Subsequent steps including dU adapter cleavage with CutSmart® buffer and column purification were stated in detail above ...
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bioRxiv - Genomics 2023Quote: ... RNA > 17 nt was isolated with the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) and separated on 15% urea-TBE gels (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... RNA > 17 nt was isolated with the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) and separated on 15% urea-TBE gels (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... and were then size-selected to isolate RNA ≥ 200 nt (DNA Clean & Concentrator™-5, Zymo Research) before reverse transcription using All-in-One cDNA Synthesis SuperMix (Bimake ...
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bioRxiv - Plant Biology 2019Quote: ... RNA greater than 200 nt was extracted using a Zymo RNA Clean & Concentrator kit (Zymo Research R1017). The obtained RNA was checked with a Bioanalyzer (Agilent ...
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bioRxiv - Genetics 2019Quote: ... RNAs longer than 200 nt were selectively recovered using the RNA Clean & Concentrator-5 kit (Zymo Research). RNA samples were fragmented and reverse transcribed ...
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bioRxiv - Synthetic Biology 2023Quote: ... 357-nt mini-PTC RNA constructs were purified with RNA Clean and Concentrator-25 columns (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction was purified and size selected to > 200 nts using Zymo Clean and Concentrator-5 (Zymo #D4013) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... followed by clean-up and enrichment of RNA > ∼150 nt using RNA clean and concentrator (Zymo Research #R1013). DNase activity was tested beforehand to ensure DNA digestion ...
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bioRxiv - Genomics 2023Quote: ... followed by gel size selection to isolate ribosome protected fragments (26-31 nt) (Zymo Research, cat. no. R1070). Following end-repair phosphorylation of RNA molecules ...
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bioRxiv - Microbiology 2021Quote: ... and RNA >200 nt was purified using an RNA Clean & Concentrator-5 Kit per the manufacturer’s instructions (Zymo Research). Ribosomal RNA was depleted using a MICROBExpress Bacterial mRNA Enrichment Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... The reaction was purified and size selected to >200 nts using Zymo RNA Clean and Concentrator-5 (Zymo #R1013) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was size-selected to ≥200 nts using the Zymo RNA Clean and Concentrator-5 kit (Zymo #R1013) according to manufacturer’s protocol and eluted in 6 µl of nuclease-free water.
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bioRxiv - Plant Biology 2020Quote: ... and RNA greater than 17 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1017). After checking digestion quality ...
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bioRxiv - Plant Biology 2020Quote: ... RNA less than 200 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1015). Next ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were run on an UltraPure Agarose (Thermo Fischer Scientific) gel and cDNA fragments larger than 500 nt were purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo Research). The library was prepared using the ONT 1D kit (SQK-LSK108 ...
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bioRxiv - Genetics 2022Quote: ... Resulting cDNA was separated on a 4% agarose gel and the 160–190 nt long fragments were extracted and gel purified using the Zymoclean Gel DNA Recovery Kit (D4008, ZYMO RESEARCH). Small RNA Libraries were sequenced in triplicates on a NovaSeq S1 SR100 mode (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... gel fragments corresponding to 190 – 350 nts in DNA size were excised and libraries extracted using the Zymoclean Gel DNA Recovery Kit (D4007, Zymo Research). DNA concentrations were measured using the Qubit dsDNA HS Assay Kit (Q32854 ...
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bioRxiv - Genomics 2022Quote: ... The corresponding amount of polyA RNA were incubated with 1X fragmentation buffer at 95 °C for 1 minute to generate ~300 nt of RNA fragment followed by column purification (Zymo Research, R1016) as described before [23] ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA). Small RNAs were prepared by GenXPro (GenXPro GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting fraction was loaded on a 20% denaturing PAGE gel after which a band of approximately 55-60 nt (Supplementary figure 2A) was excised and purified from gel using the ZR small-RNA PAGE Recovery Kit (Zymo Research, USA).
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... small RNAs ranging from 17-to-25 nt were excised and recovered using the ZR small-RNA PAGE Recovery Kit (Zymo Research, #R1070). TraPR ...
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bioRxiv - Microbiology 2022Quote: The ZymoBIOMICS human fecal reference sample (Zymo D6323) was thawed at room temperature and 100 μL was taken and centrifuged at 35g for 20 minutes to separate cells from large fecal particles ...
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bioRxiv - Neuroscience 2019Quote: ... Methylated and unmethylated human gDNAs (Zymo Research, Irvine, CA) were bisulfite-converted using the EZ DNA Methylation-Gold kit (Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... The Human Methylated & Non-methylated DNA Set (ZYMO Research) was used to generate DNA methylation standards ...
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bioRxiv - Neuroscience 2023Quote: OE from 3 controls and 3 sFlt1 mice was dissected and immediately placed in RNA lysis buffer (Zymo Research R1051) without vortexing for 5min and further processed according to manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA from six whole 20-21 dpf offspring (3 control offspring, 3 treatment offspring) was extracted using a Zymo Duet extraction kit (Zymo, NZ). The integrity of RNA samples was determined using an Agilent RNA 6000 Nano chip on an Agilent 2100 Bioanalyzer to check that the samples had an RNA Integrity Number (RIN ...
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bioRxiv - Systems Biology 2023Quote: ... 600 µL DNA/RNA wash buffer (Zymo, #D7010-3), and 400 µL DNA/RNA wash buffer ...
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bioRxiv - Genomics 2024Quote: Neutralization Buffer (Zymo Research, cat. no. D4027-3-50)
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bioRxiv - Immunology 2020Quote: ... whole zebrafish larvae or human biopsies using TRI Reagent (Zymo Research) or TRIzol (Invitrogen ...
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bioRxiv - Genomics 2021Quote: RNA from human RPTECs was extracted using the Direct-zol MicroPrep Plus Kit (Zymo) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cultured or purified human cells were lysed directly in RNA lysis buffer (Zymo Research) and homogenized by pipetting ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from primary human PTC with the Direct-zol MicroPrep Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: RNA from human RPTECs was extracted with the TRIZOL and Direct-zol MicroPrep Plus Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from ∼3×105 cells using Direct-zol mini kit (Zymo) or Trizol reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Digestion was confirmed with methylated and unmethylated human DNA standards provided with the OneStep qMethyl kit (Zymo) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Bisulfite conversion was confirmed by PCR using Universal Methylated Human DNA Standard with hMLH1 Primers (Zymo Research). Converted DNA was then applied to an Illumina Infinium MethylationEPIC850 BeadChip (865918 probes ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were isolated in a 3 mL prep using Zyppy Plasmid Miniprep kit (Zymo Research), and inserts were verified by using Sanger sequencing.
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bioRxiv - Developmental Biology 2022Quote: ... GATA-3 and RORC were generated by PCR with ZymoTaq PCR Master Mix (Zymo Research) using the primers listed in Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... samples were homogenized 3:1 weight:volume in DNA/RNA Shield® (Zymo® Research Inc.).
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bioRxiv - Cell Biology 2023Quote: ... After 3 h of growth cells were arrested with 25 μM α-factor (Zymo Research) for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted from ∼3×106 cells by using Quick-DNA Kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 and 4 by lysing cells in the 6-well plate in RNA lysis buffer (Zymo) and freezing at −80 °C until extraction ...