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Citations for Zymo Research :
1 - 50 of 50 citations for M CSF Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
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bioRxiv - Systems Biology 2019Quote: ... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
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bioRxiv - Cell Biology 2020Quote: ... cells were washed with 0.9 M sorbitol and 0.1 M EDTA and exposed to Zymolyase (Zymo Research) at 37 C° until a majority of cells burst when exposed to hypo-osmotic stress in water ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from HEK293 and CDK9as cells using a Quick-RNA Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the DNMT3A KO and WT HEK293 cells were cultured and RNA samples were extracted using Direct-zol RNA kit (Zymo Research; Irvine, CA, USA). Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 ul M-Digestion Buffer and 1ml Proteinase K (Zymo D3001-2-5) were added and incubated for 20 minutes at 50°C ...
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bioRxiv - Microbiology 2022Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Purified bisulfite-treated DNA was eluted in 15 mL of M-Elution Buffer (Zymo Research). DNA methylation levels were measured using Infinium Human Methylation 450 arrays (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were suspended in 6 ml of 1:1 mix of 1 M sorbitol: YPDplus medium (Zymo Research) and incubated at 30°C for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... 20 μL of the reaction mixture was added to 10 μL 0.5 M EDTA and purified by ZYMO Oligo Clean & Concentrator spin columns ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted by vortexing for 30 min in elution buffer (1% SDS and 0.1 M NaHCO3) and DNA was purified using a ChIP DNA Clean & Concentrator kit (Zymo Research). For each ChIP ...
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bioRxiv - Cell Biology 2021Quote: ... Spheroplasting of harvested yeast cells was performed for 2 minutes in lysis buffer (1 M sorbitol 100 mM EDTA, 0.1% β-mercaptoethanol) containing 25 units of zymolyase (Zymo Research). RNA extraction was performed with an RNeasy kit (QIAGEN ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... 300µm diameter) follwing a Proteinase K digestion (at 55°C for 2 hours) using a Quick-RNA MicroPrep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... In vitro transcripts were gel purified using 8 M urea 10% PAA and the ZR small-RNA PAGE Recovery Kit (Zymo Research). The in vitro transcribed RNAs carry three additional G nucleotides at their 5΄ ends originating from the T7 promoter ...
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bioRxiv - Developmental Biology 2021Quote: ... or Universal Methylated Mouse DNA Standard (Zymo Research) was bisulfite converted using EZ DNA Methylation-GoldTM Kit (Zymo Research) ...
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bioRxiv - Neuroscience 2020Quote: ... and sorted into 384-well plates preloaded with 2μl of digestion buffer for snmC-seq215 (20 mL digestion buffer consists of 10 mL M-digestion buffer (2×, Zymo D5021-9), 1 ml Proteinase K (20 mg ...
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bioRxiv - Genomics 2022Quote: ... separating 22 or 10 (control wells) single nuclei per well in a 96-well plate containing 2.5 μL of M-digestion buffer (Zymo, Cat. D5020-9), 0.25 μL of Proteinase K (Zymo ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction was performed from the 0.22 μm filters (containing the 5–0.22 μm microbial size fraction) using the ZR Soil Microbe DNA MiniPrep kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... Reactions were then quenched by the addition of 1 volume DTT 1 M and then purified on an RNA Clean & Concentrator-5 column (Zymo Research, cat. R1013).
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bioRxiv - Molecular Biology 2020Quote: ... Universally methylated mouse DNA standard (cat. no. D5012, Zymo Research) and Mlh1−/− tissues were used as Mlh1+/+ and Mlh1−/− allele controls ...
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bioRxiv - Molecular Biology 2020Quote: ... Universally methylated mouse DNA standard (cat. no. D5012, Zymo Research, Irvine, CA) and genomic DNA extracted from normal spleen was used as positive and negative controls ...
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bioRxiv - Cell Biology 2020Quote: ... and primary mouse keratinocytes was isolated using Direct-zol RNA Microprep Kits (Zymo) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: RNA from mouse lungs was extracted using RNA Miniprep Plus Kit (Zymo Research). Briefly ...
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bioRxiv - Cell Biology 2023Quote: gDNA was isolated from mouse gastrocnemius using Quick-DNA Microprep Plus Kit (Zymo D4074). gDNA was amplified using CloneAmp™ HiFi PCR Premix (Takara 639298 ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated from 5µl of mouse serum using the RNA Clean & Concentrator -5 (Zymo) or High Pure Viral Nucleic Acid kit (Roche ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was isolated from mouse kidneys using Direct-zolTM RNA MiniPrep Plus (Zymo Research). 1 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from mouse kidneys using Direct-zol™ RNA MiniPrep Plus (Zymo Research). 2 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research), and all of them were assessed for purity and quantity using a Nanodrop 1000 spectrophotometer ...
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bioRxiv - Molecular Biology 2021Quote: ... pLXSP-16E7 or control vector infected primary mouse keratinocytes with the Quick-RNA Miniprep kit (Zymo Research). cDNA was synthesized with the Quantinova Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: Total RNA was isolated from mouse cochlea sensory epithelia using the Direct-zolTM RNA MiniPrep (Zymo Research). The RNA was quantified using a Nano-drop® (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from mouse hearts using either the Direct-zol RNA Miniprep kit (Zymo, cat# R2051) or the RNeasy Fibrous Tissue Mini Kit (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from DIV12 infected mouse cortical neurons using the Direct-zol RNA microprep (Zymo). Ten µg were reverse-transcribed into cDNA by Maxima First Strand cDNA Synthesis Kit (Thermofischer) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse hippocampus with the Quick-RNA Miniprep Plus Kit (Zymo Research, R1058) and RNA integrity was determined using Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA (400 ng) from mouse tail was bisulphite converted with the EZ DNA Methylation-Lightning kit (Zymo Research ...
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bioRxiv - Genomics 2022Quote: ... The methylation data of each brain sample was then compared with cortex aging data of mouse by Zymo’s DNAge® predictor tool as described earlier (42).
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bioRxiv - Molecular Biology 2019Quote: Total RNA was prepared from fibroblasts isolated from mouse hearts using Direct-zol RNA miniprep kit (Zymo Research). cDNA libraries were prepared using the NEBNext Ultra Directional Library Preparation Kit following rRNA depletion using the NEB rRNA depletion kit ...
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bioRxiv - Molecular Biology 2024Quote: Mouse back skin was homogenized and RNA was extracted using Quick-RNA Miniprep Kit (Zymo Research, Cat# R1054) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from mouse iBAT following the standard protocol for the Direct-zol RNA Miniprep kit (Zymo Research) and assessed for quality on the Agilent 2200 TapeStation (Agilent Technologies) ...
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bioRxiv - Systems Biology 2022Quote: Total RNA was extracted from mouse kidneys and livers by the Direct-zol™ RNA Purification Kits (Zymo Research). Purified RNA was reverse transcribed using the SuperScript™ IV First-Strand Synthesis System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: Total RNAs were purified from each group of mouse cortex part using Direct-ZolTM RNA Miniprep Plus (Zymo research) and used for reverse transcription by SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... all mouse tissues obtained from BSL3 were treated with 1% Triton by volume or placed in TRIzol (Zymo R2050-1) to inactivate virus.
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bioRxiv - Immunology 2021Quote: ... triplicate cell samples or 50 uL fresh mouse blood were harvested into Trizol reagent before purification via Direct-zol RNA miniprep (Zymo Research) per manufacturer’s instructions with an additional dry spin after disposing of the final wash to prevent carryover ...
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bioRxiv - Microbiology 2021Quote: Infected Huh-7 cells (with or without medium) or mouse lung tissues were lysed in DNA/RNA shield reagent (Zymo Research) and total RNA was extracted by using RNeasy kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... 4sU-labelled mouse and Drosophila cells were mixed and RNA was extracted using the Direct-zol DNA/RNA Miniprep kit (Zymo Research) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was isolated from a small aliquot of mixed mouse and fly cells using Quick-DNA Miniprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from human iPSC-FBs and mouse Raw 264-7 cells using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: Fecal pellets were collected for individual mouse in each group at the same day and stored in DNA/RNA shield solution (Zymo Reasearch) at −20°C until further processing ...
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bioRxiv - Bioengineering 2022Quote: ... Total RNA was then isolated from all mouse tissues using TRIzol reagent and RNA Isolation kits with on-column DNase treatment (Zymo Cat# R2072). cDNA synthesis was performed with random primers from the Protoscript cDNA synthesis kit (NEB Cat#E6560S) ...