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1 - 6 of 6 citations for Lactose operon repressor LacI E.coli His since 2019

• A genetically engineered phage-based nanomaterial for detecting bacteria with magnetic resonance imaging

  Raymond E. Borg, et al., bioRxiv - Bioengineering 2022

  Quote: ... Reagents for making competent E.coli cells were obtained from Zymo Research (Irvine ...
• The fitness cost and benefit of phase separated protein deposits

  Natalia Sanchez de Groot, et al., bioRxiv - Systems Biology 2019

  Quote: ... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
• Nucleation of the destruction complex on the centrosome accelerates degradation of β-catenin and regulates Wnt signal transmission

  Ryan S. Lach, et al., bioRxiv - Cell Biology 2022

  Quote: All above constructs were transformed into Top10 competent cells prepared using Mix & Go E.coli Transformation Kit and Buffer set (Zymo Research #T3002), cultured on LB agar plates to select for antibiotic resistance using standard workflows for molecular cloning and DNA production 46 ...
• Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats

  Laurens H. Lindenburg, et al., bioRxiv - Biochemistry 2020

  Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
• Point mutations in topoisomerase I alter the mutation spectrum in E. coli and impact the emergence of drug resistance genotypes

  Amit Bachar, et al., bioRxiv - Microbiology 2019

  Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
• An essential host dietary fatty acid stimulates TcpH inhibition of TcpP proteolysis enabling virulence gene expression in Vibrio cholerae

  Lucas M. Demey, Ritam Sinha, Victor J. DiRita, bioRxiv - Microbiology 2022

  Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...