Labshake search
Citations for Zymo Research :
1 - 50 of 279 citations for Enolase 1 Alpha enolase Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... coli DH5 Alpha Competent Cells (Zymo Research) per the manufacturer’s instructions and isolated using a QIAprep Spin Miniprep Kit (QIAGEN) ...
-
bioRxiv - Bioengineering 2024Quote: ... and bacterial transformations (Mix & Go! Competent Cells - DH5 Alpha, Zymo). DNA constructs were sequence confirmed by Sanger Sequencing (Elim Bio ...
-
bioRxiv - Systems Biology 2019Quote: ... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... Products were transformed into Mix and Go! DH5 alpha competent cells (Zymo). Clones were selected by ampicillin resistance on Amp-LB plates (60mg/mL) ...
-
bioRxiv - Genomics 2022Quote: ... were synchronized in either G1 with alpha-factor (Zymo Research, CAT. Y1001), or in late anaphase ...
-
bioRxiv - Pathology 2023Quote: ... Calculations of alpha and beta diversity were done by standard methodology by Zymo Research.
-
bioRxiv - Cell Biology 2020Quote: ... Plasmids were propagated by transforming Escherichia Coli (Mix&Go! Competent Cells - DH5 Alpha, Zymo Research) using standard procedures ...
-
bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
-
bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
-
bioRxiv - Microbiology 2022Quote: The ZymoBIOMICS human fecal reference sample (Zymo D6323) was thawed at room temperature and 100 μL was taken and centrifuged at 35g for 20 minutes to separate cells from large fecal particles ...
-
bioRxiv - Microbiology 2022Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
-
bioRxiv - Neuroscience 2019Quote: ... Methylated and unmethylated human gDNAs (Zymo Research, Irvine, CA) were bisulfite-converted using the EZ DNA Methylation-Gold kit (Zymo Research ...
-
bioRxiv - Genomics 2021Quote: ... The Human Methylated & Non-methylated DNA Set (ZYMO Research) was used to generate DNA methylation standards ...
-
bioRxiv - Immunology 2020Quote: ... whole zebrafish larvae or human biopsies using TRI Reagent (Zymo Research) or TRIzol (Invitrogen ...
-
bioRxiv - Genomics 2021Quote: RNA from human RPTECs was extracted using the Direct-zol MicroPrep Plus Kit (Zymo) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Cultured or purified human cells were lysed directly in RNA lysis buffer (Zymo Research) and homogenized by pipetting ...
-
bioRxiv - Genomics 2024Quote: Total RNA was extracted from primary human PTC with the Direct-zol MicroPrep Kit (Zymo) following manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: RNA from human RPTECs was extracted with the TRIZOL and Direct-zol MicroPrep Plus Kit (Zymo) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Digestion was confirmed with methylated and unmethylated human DNA standards provided with the OneStep qMethyl kit (Zymo) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Bisulfite conversion was confirmed by PCR using Universal Methylated Human DNA Standard with hMLH1 Primers (Zymo Research). Converted DNA was then applied to an Illumina Infinium MethylationEPIC850 BeadChip (865918 probes ...
-
bioRxiv - Microbiology 2022Quote: For human OE and OB RNA was extracted from 10mg of tissue per sample using Direct-zol RNA kit from Zymo Research (Catalog #R2052) ...
-
bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from human iPSC-FBs and mouse Raw 264-7 cells using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instructions ...
-
bioRxiv - Genomics 2023Quote: ... 350 ng of each extracted sperm DNA and 350 ng of control human NA12878 lymphoblastoid cell line genomic DNA (Coriell Institute) were bisulfite-converted using the Zymo EZ DNA Methylation kit (Zymo Research). The loci were amplified by PCR using the following primer sets ...
-
bioRxiv - Cancer Biology 2023Quote: RNA from exponentially growing or senescent human and bowhead whale primary skin fibroblasts was isolated using the Quick-RNA MiniPrep kit (Zymo Research) according to manufacturer instructions.
-
bioRxiv - Molecular Biology 2023Quote: ... 1 ml Trizol (R2050-1, Zymo Research) as well as 200 µl chloroform were added to the interface and phase separation and removal were repeated ...
-
bioRxiv - Genomics 2019Quote: ... RNA was isolated from different number of murine splenic CD3 positive T cell samples and human oocyte samples by using the Direct-zol™ RNA MiniPrep Kit (ZYMO, cat. R2050). The extracted RNA samples were dissolved into 30 µl of nuclease-free water (Qiagen ...
-
bioRxiv - Genetics 2020Quote: ... Two representative loci per fungal strain and the human MLH1 for the control were amplified by PCR (ZymoTaq PreMix, Zymo Research, Irvine, CA) using specifically designed primers for amplification of the bisulfite-treated DNA (designed using the ‘Bisulfite Primer Seeker’ (Zymo Research ...
-
bioRxiv - Genomics 2022Quote: Total RNA was isolated from LV apical core tissue and from beating human iPS cell derived cardiomyocytes using Quick-RNA Miniprep Plus (Zymo Research, Irvine, CA, USA). The qRT-PCR was performed using SYBR mix (Thermofisher ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were suspended in 6 ml of 1:1 mix of 1 M sorbitol: YPDplus medium (Zymo Research) and incubated at 30°C for 1 h ...
-
bioRxiv - Immunology 2021Quote: ... filled with 1% FBS-HBSS or Tri Reagent (Zymo, #R2050-1-200). Tissue was homogenized in an Omni Bead Ruptor 24 (5.15 ms ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were directly lysed in 1 ml of Trizol (R2050-1, Zymo Research) and subjected to interface cleanup as described below.
-
bioRxiv - Molecular Biology 2023Quote: ... Round 1 PCRs were cleaned using DNA Binding Buffer (Zymo ZD4004-1-L) and UPrep Micro Spin Columns (Genesee Scientific 88–343) ...
-
bioRxiv - Microbiology 2023Quote: ... For the ITS and 16S PCRs a dilution series (1□10−1-1□10−3) of ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Germany) was included in duplicate ...
-
bioRxiv - Neuroscience 2021Quote: ... or TRI Reagent (Zymo, #R2050-1) for gene expression analysis ...
-
bioRxiv - Genomics 2021Quote: ... digestion with 1% zymolyase (Zymo Research) to weaken ascus walls ...
-
bioRxiv - Microbiology 2020Quote: An aliquot of each fraction was then mixed 1:1 with 2× DNA/RNA Shield (Zymo Research) spiked with in vitro transcribed RNA (generated by T7 transcription off a Firefly luciferase (Fluc)-containing plasmid) ...
-
bioRxiv - Cancer Biology 2020Quote: Approximately 1.4kb of genomic fragment spanning SALL4 5’ UTR - exon 1 - intron 1 was PCR amplified (Zymo Research) and cloned into the pGEM-T Easy vector ...
-
bioRxiv - Molecular Biology 2023Quote: ... Infectious viral stocks were inactivated by a 1:3 dilution with TRI Reagent® (Zymo, R2050-1-200) immediately prior to RNA extraction ...
-
bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from mixtures of KU-1 or BL3.1 and BTL26 for a total of 1 × 106 cells using Quick-DNA Miniprep Kits (Zymo Research). The DNA concentrations of the samples were measured by UV absorbance at 260 nm using a NanoDrop 8000 Spectrophotometer (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2023Quote: ... all mouse tissues obtained from BSL3 were treated with 1% Triton by volume or placed in TRIzol (Zymo R2050-1) to inactivate virus.
-
bioRxiv - Bioengineering 2022Quote: ... and 1 mg/ml Proteinase K (Zymo Research) for 1 hour at 55°C ...
-
bioRxiv - Microbiology 2021Quote: ... A 10:1 RNA/DNA shield from Zymo was added to each saliva sample to maintain samples during shipping.
-
bioRxiv - Microbiology 2022Quote: ... and 100μL TRIzol (Zymo Research, R2050-1-50) was added to each well followed by isolation of total RNA using Direct-Zol RNA Mini-Prep Plus Kit (Zymo Research ...
-
bioRxiv - Immunology 2020Quote: PCR testing for SARS-CoV-2 was carried out on respiratory specimens mixed 1:1 in DNA/RNA Shield (Zymo Inc) using an in-house Clinical Laboratory Improvement Amendments (CLIA ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were treated with RNase for 1 hour and proteinase K for 1 hour before ChIP DNA Clean & Concentrator (Zymo Research) to extract the DNA.
-
bioRxiv - Developmental Biology 2022Quote: ... 1 x ZymoTaq PreMix (Zymo Research, Irvine CA, USA) and 10μM of each primer ...
-
bioRxiv - Genomics 2020Quote: ... with Oligo binding buffer (Zymo Research, D4060-1-10). The final product was assumed to have full yield (for a list of sequences ...
-
bioRxiv - Cancer Biology 2022Quote: ... then homogenized with TRI Reagent (Zymo Research, R2050-1) by the gentleMACS Dissociator (Miltenyi Biotec 130-093-235) ...