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Citations for Zymo Research :
1 - 50 of 102 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 10 μL of 2X ZymoTaq PCR Mix (Zymo Research), PCR primers ...
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bioRxiv - Developmental Biology 2022Quote: ... GATA-3 and RORC were generated by PCR with ZymoTaq PCR Master Mix (Zymo Research) using the primers listed in Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 2X RNA Binding Buffer (Zymo Research) was added and vortexed for 10 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... 2X RNA Binding Buffer (Zymo Research) was added and vortexed for 10 seconds ...
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Systems Biology 2023Quote: ... and 2x 400uL RNA Wash Buffer (Zymo Research), spinning each time at 10,000x g for 20 seconds ...
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bioRxiv - Zoology 2022Quote: ... Libraries with low 260/230 ratios (< 1.8) or low concentration were re-purified using an RNA Clean and Concentrator kit (Zymo). RNA libraries were diluted to 20 ng μL−1 and reverse transcription was performed with an iScript cDNA synthesis kit (Bio-Rad) ...
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bioRxiv - Genomics 2021Quote: ... containing 600 µL of 2x DNA/RNA shield (Zymo Research). gentleMACS M tubes were then placed on the gentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Pathology 2020Quote: ... and 50 µL of 2X DNA/RNA Shield (Zymo Research, Irvine CA) in a total reaction volume of 100 µL ...
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bioRxiv - Microbiology 2023Quote: ... low yield integrating plasmids were midi or maxiprepped (Zymo Research #4200 / #4202) using the low copy number protocols ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 ng of RNA per sample was used in each qPCR reaction according to instruction in the ZymoScript Onestep RT-qPCR Kit (Zymo Research). qPCR was conducted using a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... Mix&Go transformation reagents and Mix&Go JM108 chemically competent cells were obtained from Zymo Research (Irvine ...
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bioRxiv - Bioengineering 2022Quote: ... Mix and Go competent cells (Zymo Research), QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... The ligation mix was purified (Zymo Research) and 2 μL of the purified reaction mix was used to transform into 50 μL of chemically competent E ...
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bioRxiv - Plant Biology 2021Quote: ... coli (Zymo Research Mix & Go! Competent Cells), PCR-positive colonies were used for plasmid purification before Sanger sequencing (Eurofins ...
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bioRxiv - Systems Biology 2023Quote: ... 3 μl of reaction mix were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Microbiology 2023Quote: ... 4 ml of urine was added to 4 ml of 2X DNA/RNA Shield® (Zymo, USA). Serum and urine samples were stored and transported on ice to the Institute of Microbiology at Universidad San Francisco de Quito and thereafter maintained at −20°C until DNA extraction.
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bioRxiv - Molecular Biology 2024Quote: ... or Mix & Go Stbl3 competent cells (Zymo Research). Following standard plasmid DNA preparation ...
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bioRxiv - Microbiology 2021Quote: ... coli competent cells Mix & Go Zymo 5α (Zymo research) according to product manuals ...
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bioRxiv - Microbiology 2022Quote: ... Ligations were transformed into competent Mix&Go! (Zymo Research) Top10 E ...
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bioRxiv - Molecular Biology 2023Quote: ... 5hmdCTP/dGTP/dATP/dTTP mix was purchased from Zymo Research ...
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bioRxiv - Microbiology 2021Quote: ... the pelleted cells and debris were resuspended in 500 μL of 2x DNA/RNA Shield (Zymo Research (Irvine, CA) and stored at –20°C ...
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bioRxiv - Developmental Biology 2020Quote: RNA for WT and LSD1KO crypts was isolated by sorting IECs (DAPI-, Epcam+) in 2x RNA shield buffer (Zymo) and RNA isolation using the Quick-RNA Micro prep kit (Zymo) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Transformation was performed using the Mix & Go kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli Mix & Go! Competent Cells-Zymo 10B (Zymo Research, T3020). For assemblies using destination vectors possessing the R6Kγ ORI ...
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bioRxiv - Bioengineering 2024Quote: ... and bacterial transformations (Mix & Go! Competent Cells - DH5 Alpha, Zymo). DNA constructs were sequence confirmed by Sanger Sequencing (Elim Bio ...
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bioRxiv - Cancer Biology 2022Quote: Chromatin immunoprecipitation and qPCR was performed using the Zymo-Spin ChIP kit (Zymo, D5209) as previously described (24) ...
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bioRxiv - Genetics 2020Quote: ... we used DH5alpha Mix & Go Competent Cells (Zymo Research, cat. T3007) in all the above clonings except for the his-72::GFP repair template which required ccdB resistant bacteria for which we used One Shot ccdB Survival (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... coli generated from the Mix and Go Transformation Buffer kit (ZYMO). Plasmids were sequenced for validation.
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bioRxiv - Systems Biology 2024Quote: ... 2uL was transformed into 10beta Mix-and-Go chemicompetent cells (Zymo), and cells were plated to select on LB+Carb plates ...
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bioRxiv - Genetics 2023Quote: For transformation into Zymo Mix & Go DH5α competent cells (Zymo T3007), 100 µL of cells were thawed on ice per reaction ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA for RT-qPCR was isolated using Direct-zol RNA MiniPrep Kit (Zymo Research) for all other experiments RNA was isolated using standard Tri-reagent (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... from weight-normalized contents from the distal small intestine and proximal colon after addition of Low Abundance Microbiota Standard (Zymo) and extraction using the Zymo DNA Mini kit (Zymo) ...
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bioRxiv - Genomics 2020Quote: ... 2.5 μl of 10 mM 5-Methylcytosine dNTP mix (Zymo Research, D1030), and incubated at 15 °C for one hour ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Products were transformed into Mix and Go! DH5 alpha competent cells (Zymo). Clones were selected by ampicillin resistance on Amp-LB plates (60mg/mL) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli DH5α (Zymo Mix & Go E. coli Transformation Kit, Zymo Research #T3001). Each construct was verified by Sanger sequencing (Australian Genome Research Facility ...
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bioRxiv - Microbiology 2022Quote: ... coli XL1-Blue Mix & Go competent cells (Zymo Research, cat. no. T3002) or E ...
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bioRxiv - Neuroscience 2023Quote: ... We transformed infusion reactions into Mix N’ Go (Zymo Research catalog # T3001) chemically competent Stbl3 E ...
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bioRxiv - Biophysics 2023Quote: ... coli Mix&Go Transformation kit with Zymobroth following manufacturer’s instructions (Zymo, T3001), hereafter referred to as Home-Made Competent Cells (HMCC) ...
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bioRxiv - Molecular Biology 2023Quote: ... of 0.4–0.6 and made competent using a Mix & Go kit (Zymo). Immediately after ...
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bioRxiv - Genomics 2023Quote: qPCR for bacterial DNA load was performed using the Femto Bacterial DNA Quantification kit (Zymo Research). 2 μL of eluate was used as the input ...
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bioRxiv - Microbiology 2020Quote: The respiratory samples were prospectively stored at −80°C after stabilization by mixing an equal volume of DNA/RNA Shield (2X concentrate; Zymo Research, Irvine, CA). The thawed samples were centrifuged at 20,000×g for 2 min ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified by gel extraction from a 1.5 % low-melt agarose gel using a Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Samples were quantified using the Qubit dsDNA HS assay (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The amplified products were purified using the QIAquick PCR purification kit and underwent size selection using 1.5% low-melt agarose gels and Gel DNA Recovery Kit (Cat. No. D4002, Zymo Research, Irvine, CA). A final pool was made after quantification of each sample using the Qubit high-sensitivity dsDNA kit (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... Obtained sgRNA mix was purified using RNA Clean and Concentrator-5 (Zymo Research). Injections were performed as before with the exception that 60 μM sgRNA mix was combined with diluted 10 μM Cas9 protein to reach final ∼5 μM RNP complex concentration (molar ratio 6:1 ...
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bioRxiv - Microbiology 2024Quote: ... and transformed into BW25113 using a Mix and Go Transformation kit (Zymo Research) as per the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were then transformed into Zymo Mix & Go DH5α Competent Cells (Zymo T3007) for verification and amplification before re-purification and transformation into MG1655 E ...
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bioRxiv - Neuroscience 2019Quote: ... and via RT-qPCR with a next generation sequencing (NGS) library quantification kit (Zymo Research, Irvine, CA). RNA sequencing (1 lane ...
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bioRxiv - Cell Biology 2019Quote: ... washed twice with ice cold PBS and processed for qPCR using a RNA purification kit (Zymo Cat# D7011). 500 ng total RNA from each sample were reverse transcribed using Superscript™ III reverse transcriptase using an oligo(dT ...