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Citations for 3M :
1 - 50 of 194 citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Cells are then resuspended in 10ml Sucrose/Glycerol buffer (1:1) (Sucrose Buffer: 0.3M Sucrose; 1% Triton-X (Sigma Aldrich); 10mM HEPES (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated in blocking solution (1% BSA, 0.3M glycine in DPBS) for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... 300 mM Imidazole and 5% glycerol and the buffer was exchanged against a stabilization buffer (50 mM Bis-TRIS propane pH6.5, 0.3M NaCl, 5% glycerol) via gravity using a desalting column (PD10 ref ...
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bioRxiv - Cell Biology 2020Quote: ... cells were lysed with 1 x SDS sample buffer (0.32 M Tris pH 6.8, 10% SDS, 50% glycerol, 3M β-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... The nuclei were incubated with blocking buffer (1% Normal Donkey Serum in PBS + 0.3M glycine) for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... using (10mM HEPES, pH7.5, 0.3M NaCl, 10% glycerol, 1mM TCEP).
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bioRxiv - Genetics 2021Quote: ... and then incubated again in blocking solution (0.1% Triton-× 100, 1:50 goat serum, 0.3M glycine) for 30 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... Then the cells were incubated with blocking buffer (1XPBS, 1% BSA, 0.3M glycine, 0.1% Tween 20) supplemented with 5% goat serum for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were desalted with C18 (3M Empore) desalting tips.
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bioRxiv - Physiology 2019Quote: ... Sections were incubated in blocking solution (1%BSA: Sigma Aldrich, 10% normal horse serum: ThermoFisher Scientific, UK 0.3M glycine ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 1 hour at room temperature in Blocking Solution (PBS containing 1% BSA, 10% FBS, 0.3M glycine and 0.1% Tween-20). The fixed cells were incubated with a mixture of the primary antibodies in PBS overnight at 4°C ...
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bioRxiv - Genetics 2023Quote: ... Fixed cells were incubated in blocking buffer (1% BSA, 2% FCS, 0.3M glycine and 0.1% Tween-20 in PBS) at room temperature for 1 hour ...
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bioRxiv - Systems Biology 2020Quote: ... Blocking buffer (0.3M glycine buffer, 10% Goat Serum, 1XPBS) was added to ‘dry’ pellets on ice for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Fixed cells were first permeabilized for 10 minutes in 0.2 % Triton X solution and subsequently incubated for 1 hour at RT in blocking solution (1% BSA, 5% donkey serum, 0.3M glycine and 0.02% Triton X in PBS). Following blocking ...
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bioRxiv - Molecular Biology 2020Quote: ... equilibrated and eluted with SEC buffer (10mM HEPES, pH8.0; 0.3M NaCl, 10% glycerol, 1mM TCEP). The BTB domain of PLZF (Ahman ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped by acidification with TFA (1% [v/v] final concentration) and the peptide sample was desalted using a EmporeTM Solid Phase Extraction cartridge (3M Bioanalytical Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Digested peptides were sequentially desalted using C18 Stagetips (3M Empore) and porous carbon material (TipTop Carbon ...
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bioRxiv - Systems Biology 2021Quote: ... The digested peptides were desalted on C18 stage tips (3M) and dried with Speed-Vac (Thermo) ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were desalted using C18 (3M Empore, 66883-U) StageTips.42 After quantification by Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Isolated tryptic peptides were desalted by C18 SPE (Empore, 3M), dried under vacuum ...
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bioRxiv - Systems Biology 2023Quote: ... Digested peptides were desalted using 1ml Empore C18 cartridges (3M) before isobaric labeling.
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear extracts were extracted at 4 C in high salt buffer (20mM Tris pH7.9, 1.5mM MgCl2, 25% glycerol, 0.2mM EDTA, 0.5mM DTT and 0.3M NaCl) followed by centrifugation at 6000g ...
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bioRxiv - Biochemistry 2021Quote: ... Trifluoroacetic acid (0.1 %) was added to the digested peptides and desalted using StageTips packed with a solid phase C18 column disk (3M Empore) with porous graphitic carbon (PGC ...
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bioRxiv - Genomics 2019Quote: ... Slides were washed three times with PBS and incubated with blocking solution (1% Bovine serum albumin, 10% Donkey serum, 0.3M Glycine, 0.1% PBS Tween) for 1 hour at room temperature followed by primary antibody in blocking solution overnight at 4 degrees (see Table 1 for source ...
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bioRxiv - Microbiology 2022Quote: ... aureus or PBS with 10% glycerol vehicle control and the wound covered with transparent film (Tegaderm, 3M). Each wound was photographed and measured at the time of wounding (t=0) ...
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bioRxiv - Cell Biology 2020Quote: ... lysed in protein sample buffer (0.2M Tris HCl, 0.4 M DTT, 277 mM SDS, 6mM Bromophenol Blue, 4.3M glycerol), and boiled and subjected to SDS-PAGE and Western blotting as described above.
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was washed once with Disruption Buffer (20mM Tris-HCl pH7.9, 10mM MgCl2, 1mM EDTA, 10% glycerol, 0.3M ammonium sulfate and protease inhibitor) ...
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bioRxiv - Cell Biology 2021Quote: ... Digested peptides were desalted using self-packed C18 STAGE tips (3M EmporeTM) (Rappsilber et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were desalted using 47-mm SDB-RPS disks (3M, Saint Paul). The extraction disks were washed twice with 0.2% trifluoroacetic acid (TFA) ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were desalted using C18 Empore® extraction discs (3M, Maplewood, MN) STAGE tips (Rappsilber et al. ...
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bioRxiv - Microbiology 2022Quote: ... The peptides were purified using C18 stage-tips (Empore SPE disks, 3M) [78] and measured on a Q-Exactive plus mass spectrometer (Thermo-Fisher ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: Peptides were loaded onto styrenedivinylbenzene–reversed phase sulfonated (SDB-RPS; 3M Empore) StageTips in 100 μl SDB-RPS wash buffer (0.2 % TFA) ...
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bioRxiv - Microbiology 2023Quote: ... The digested peptides were desalted and concentrated using POROS C18 (Empore, 3M) and eluted directly onto the MALDI plate using 1 μL of 5 mg/mL alpha-cyano-4-hydroxycinnamic acid (CHCA ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were purified on StageTips with three C18 Empore filter discs (3M) and analyzed by MS as described previously (61) ...
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bioRxiv - Immunology 2023Quote: ... The peptides were extracted and desalted with C18 Disk Filters (3M Empore).
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bioRxiv - Molecular Biology 2022Quote: ... Samples were fixed with ice cold methanol for 5 min and then in blocking buffer (1% BSA, 10 % Horse serum, 0.3M Glycine, 0.2% Triton X in PBS) for 1 h RT ...
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bioRxiv - Biochemistry 2021Quote: ... The crude F1 preparation was loaded onto a 5mL HisTrap HP 5 mL column at 4 °C and washed with 300 mL of 97 % wash buffer (10% MeOH, 10% glycerol, 0.3M NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... and permeabilized by incubation in blocking buffer (0.05M PBS, 0.3M glycine, 5% donkey serum) containing one of two detergents ...
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bioRxiv - Systems Biology 2020Quote: ... Peptides were desalted using in-house prepared C18 (3M Empore, Maplewood, MO, USA) tips and were reconstituted in 0.5% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide mixtures were desalted and fractionated using self-packed C18 (3M Empore) stage tips filled with 3 μL of porous R3 resin (Applied Biosystems) ...
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bioRxiv - Systems Biology 2021Quote: ... Peptide purification on StageTips with three layers of C18 Empore filter discs (3M) and subsequent mass spectrometry analysis was performed as described previously55,56 ...
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bioRxiv - Systems Biology 2021Quote: ... Peptide purification on StageTips with three layers of C18 Empore filter discs (3M) and subsequent mass spectrometry analysis was performed as described previously55,56 ...
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bioRxiv - Plant Biology 2022Quote: ... Peptide samples were desalted using stage-tips (SDB-RPS, 3M, Saint Paul, USA). Samples were spun and loaded in stage tips (4 punches of SDB membrane used in 200 μl pipette tips) ...
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bioRxiv - Microbiology 2023Quote: ... Digested peptides were subsequently desalted using self-packed C18 STAGE tips (3M EmporeTM) 57 for LC-MS/MS analysis.
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bioRxiv - Microbiology 2023Quote: ... Peptides were purified on stage tips with three C18 Empore filter discs (3M) and analyzed by liquid chromatography coupled to mass spectrometry on a QExactive HF instrument (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... the peptides were desalted employing the Stage Tip approach with C18 membranes (3M) and following the procedure described by Rappsilber et al ...
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bioRxiv - Plant Biology 2023Quote: ... the resulting peptides were purified using self-packed StageTips (C18 material, 3M Empore) and analyzed by mass spectrometry ...
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bioRxiv - Cell Biology 2021Quote: ... washed with water and resuspended in glass bead disruption buffer (20mM Tris-HCl pH 8.0, 10mM MgCl2, 1mM EDTA, 5% Glycerol, 0,3M ammonium sulfate) supplemented with 1mM DTT ...
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bioRxiv - Molecular Biology 2020Quote: Purified His6-KIAA1841(BTB) protein at 0.1mg/ml in sample buffer (10mM HEPES, pH8.0; 0.3M NaCl, 10% glycerol, 1mM TCEP) was crosslinked with 5-fold increasing concentration of glutaraldehyde (i.e ...
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bioRxiv - Biophysics 2022Quote: ... The resulting tryptic peptides were desalted using C18 stage tips (homemade, 3M company, USA) dried and resuspended in 0.1% Formic acid ...