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Citations for Electron Microscopy Sciences :
1 - 50 of 63 citations for Prestained Protein Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were cleaned by standard acetone precipitation (EM grade acetone; EMS). The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: Standard plain carbon grids (copper 300 mesh, vendor: EMS) were glow discharged in air plasma (30s ...
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bioRxiv - Genomics 2020Quote: ... The standard procedure of infiltration and embedding using Durcupan resin (EMS) was performed ...
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bioRxiv - Neuroscience 2020Quote: ... mice were euthanized by standard transcardial perfusion using 4% PFA (PFA, Electron Microscopy Sciences) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... processed in a standard manner and embedded in EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). 70 nm ultrathin sections were cut (UC6 microtome ...
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bioRxiv - Cell Biology 2020Quote: ... then processed in a standard manner and embedded in EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). Ultrathin sections (60 nm ...
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bioRxiv - Biophysics 2019Quote: ... operating at 80 kV and 12 μA on standard formvar coated TEM grids (Electron Microscopy Sciences). Luminescence spectra were acquired on a modified PTI fluorimeter ...
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bioRxiv - Cell Biology 2023Quote: ... then processed in a standard manner and embedded in EMbed 812 (Electron Microscopy Sciences, Hatfield, PA). Semi-thin sections were cut at 1 μm and stained with 1% Toluidine Blue to evaluate the quality of preservation and find the area of interest ...
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bioRxiv - Neuroscience 2024Quote: ... A standard rOTO en bloc staining protocol39 was applied including postfixation in 2% osmium tetroxide (EMS), 1.5% potassium ferricyanide (Sigma ...
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bioRxiv - Microbiology 2023Quote: Protist pellets were prepared using standard methods for the Embed 812 resin kit (Electron Microscopy Sciences, EMS)60 ...
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bioRxiv - Microbiology 2023Quote: Protist pellets were prepared using standard methods for the Embed 812 resin kit (Electron Microscopy Sciences, EMS)60 ...
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bioRxiv - Epidemiology 2019Quote: ... The database complies with the National Emergency Medical Services Information System (NEMSIS) standards (http://www.nemsis.org/.) Information about incidents and patients is entered into the database by EMS crews in real time or nearly so ...
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bioRxiv - Neuroscience 2023Quote: ... 70 nm sections were cut and deposited on 200 mesh copper London Finder Grids coated with standard thickness formvar and carbon (EMS). The grids were counterstained with 4% Uranyl Acetate in 50% Ethanol and Lead Citrate (Venable and Coggeshall ...
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bioRxiv - Molecular Biology 2019Quote: ... This pixel size is in agreement with the results of the calibration procedure using Magical Reference Standard for TEM (Electron Microscopy Sciences). The reported overall resolutions for TMV of 2.1 Å in Ca2+/acidic pH and 2.0 Å in water conditions ...
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bioRxiv - Cell Biology 2019Quote: ... 15 nm protein A-coated gold beads (EMS) were adhered to the grids prior to Reynolds’ lead citrate staining ...
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bioRxiv - Molecular Biology 2023Quote: ... 3µL of protein was applied to Quantifoil grids (EMS) that were glow-discharged for 30s at 15 mA ...
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bioRxiv - Neuroscience 2020Quote: ... and then deeply anesthetized by Fatal-plus injection (0.075 mL of 97.5 mg/mL solution in NaCl) followed by a standard transcardial perfusion using PBS (50 mL) followed by PFA (Electron Microscopy Sciences, 50 mL, 4% in PBS).
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bioRxiv - Microbiology 2020Quote: ... Each protein was deposited on a CF-400-CU grid (Electron Microscopy Sciences) that had been plasma cleaned for 30 seconds in a Solarus 950 plasma cleaner (Gatan ...
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bioRxiv - Synthetic Biology 2020Quote: Purified protein samples applied to CF300-Cu carbon coated grids (Electron Microscopy Sciences) were prepared for negative stain transmission electron microscopy ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of protein vesicle solution was dropped on a copper grid (Electron Microscopy Sciences) for 5 min at 25 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Diluted protein was adsorbed on freshly glow-discharged carbon-film grid (CF200-CU, Electron Microscopy Sciences), washed with dilution buffer and stained with 0.75% w/v uranyl formate ...
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bioRxiv - Microbiology 2021Quote: ... 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 mA (Tedpella Inc) ...
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bioRxiv - Neuroscience 2021Quote: ... the beads with enriched proteins were washed three times with 80% ethanol (Electron Microscopy Sciences Cat. #15055) followed by incubation with 100 μL 50mM ammonium bicarbonate (MilliporeSigma Cat ...
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bioRxiv - Neuroscience 2021Quote: Protein samples were deposited on formvar/carbon-coated 200-mesh copper grids (FCF200-Cu, Electron Microscopy Sciences) for 1 min at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... A 2.2µL drop of protein was deposited on a Quantifoil R1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Biochemistry 2022Quote: ... A 3.5 μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences) that had been glow discharged for 10 seconds using an easiGlow Glow Discharge Cleaning System (PELCO) ...
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bioRxiv - Biochemistry 2022Quote: ... A 2.3-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Microbiology 2021Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 seconds using a PELCO easiGlow™ Glow Discharge Cleaning System ...
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bioRxiv - Neuroscience 2022Quote: ... EGFP expression in OB was detected using a dual fluorescent protein flashlight (Electron microscopy sciences, Hatfield, PA, USA) prior to surgery ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3 μl of protein was applied to glow-discharged carbon-coated copper grids (EMS; CF-400-Cu) and incubated for 45 s at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of protein sample was applied to freshly-ionized carbon-coated copper mesh grids (Electron Microscopy Science). After 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of protein was loaded onto a glow-discharged CF200-CU carbon grid (Electron Microscopy Sciences, U.K.). After 5 min of incubation ...
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bioRxiv - Immunology 2023Quote: ... A 2.4-μL drop of protein was deposited on a Quantifoil-1.2/1.3 grid (Electron Microscopy Sciences, PA) that had been glow discharged for 10 s using a PELCO easiGlow Glow Discharge Cleaning System ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of protein solution were applied to glow-discharged carbon coated copper grid (300 mesh, Electron Microscopy Science) and let adsorb for 30 s ...
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bioRxiv - Biophysics 2023Quote: A volume of 3.5 μl of purified channel protein was applied onto glow-discharged copper holey carbon grids R1.2/1.3 (Q350CR1.3, Electron Microscopy Sciences), which was cleaned for 1 minute in an H2/O2 plasma using a Gatan Solarus 950 (Gatan) ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of the protein solution was applied to a glow-discharged Formvar-carbon grid (EMS FCF200-Cu-UB) for 1 min followed by blotting ...
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bioRxiv - Biophysics 2021Quote: A 10 μl volume of 5 times diluted aggregated sample (initial αS concentration = 50 µM and N-protein concentration = 0.2 – 1 μM) was deposited onto freshly cleaved mica (Muscovite mica, V-1quality, EMS, US) and left to rest for 5 min ...
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bioRxiv - Biophysics 2021Quote: The nanodisc incorporated protein was individually applied to freshly glow-discharged Quantifoil R1.2/1.3 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 4 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μl of protein solution were applied onto the glow discharged Quantifoil Cu R1.2/1.3 200 grids (Electron Microscopy Sciences). The grids were blotted for 3s and plunge frozen into liquid ethane using a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: A protein sample at 1 mg/ml concentration was plunge-frozen on 200 mesh quantifoil R1.2/1.3 grids (Electron Microscopy Sciences) using a Mark IV Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: 3 μl of proteins (~0.05 mg/ml) diluted with PBS buffer were deposited onto carbon-coated grids (EMS CF200-Cu) glow-discharged for 15 seconds at 25 mA in air ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
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bioRxiv - Biophysics 2021Quote: The purified bison NHA2ΔN protein was individually applied to freshly glow-discharged Quantifoil R2/1 Cu300 mesh grids (Electron Microscopy Sciences). Grids were blotted for 3.0 s or 3.5 s under 100% humidity and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Diluted S protein was mixed with a 1.5-fold molar excess of ACE2 and the mixture was incubated on ice for 1 minute before 4.8 uL of the protein mixture was deposited on a CF400-Cu grid (Electron Microscopy Sciences) before being stained with methylamine tungstate (Nanoprobes) ...
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bioRxiv - Cell Biology 2021Quote: Exocyst holocomplex was mixed with liposomes composed of 95 mol% POPC and 5 mol% PI(4,5)P2 and protein A coated 5 nm gold fiducials (Electron Microscopy Sciences) for 3 minutes and to a final protein concentration of 1 μM ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in gels were transferred to nitrocellulose membranes and subsequently immobilized by 0.1% glutaraldehyde (15 min) (Electron Microscopy Sciences; Hatfield, PA). After blocking in Tris-Buffered Saline containing 0.1% Tween 20 (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... Cryo-EM grids were prepared by applying 3.5 μl of protein to the glow-discharged Quantifoil R1.2/1.3 200-copper mesh grids from Electron Microscopy Science (Q2100-CR1.3). The grids were blotted for 3 s ...