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Citations for Electron Microscopy Sciences :
1 - 50 of 732 citations for L Alanine N T Boc 2 13C 98 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Coronal sections (n=2 mice, P30) and whole mounts (n=2 mice, P28) were incubated with Immunosaver (1:200; EMS, Fort Washington, PA) for 20 min at 60°C ...
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bioRxiv - Biophysics 2022Quote: C-flat™ EM (CF-2/1-4C-T, Electron Microscopy Sciences) grids were glow-discharged for 15 seconds on a Pelco Easiglow at 15mW power ...
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bioRxiv - Neuroscience 2023Quote: ... and mounted on a poly-L-lysine coated coverslip using Fluoromont-G T (Electron Microscopy Science, USA). Alexa Fluor conjugated secondary antibodies (Alexa Fluor 488 conjugated goat anti-guinea pig ...
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bioRxiv - Cell Biology 2019Quote: HEK293 Flp-ln T-REx cells were grown on poly-L-lysine-coated #1.5 glass coverslips (Electron Microscopy Sciences). Cells were treated with 1 μg/mL doxycycline for 24 h to induce expression of BirA* or BirA*-CNAα ...
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bioRxiv - Evolutionary Biology 2021Quote: ... post fixed them for 2 hours in 1% osmium tetroxide (Electron Microscopy Sciences, cat n. 19150) 0.2 cacodylate sucrose buffer and washed them 1 time with buffer ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% NP-40) to glow-discharged C-Flat holey carbon grids (CF-2/1-3C-T, EMS). The samples were plunge-frozen using a Vitrobot (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: A 80 × 50 × 20 mm (L × W × T) block of PMMA (Acrylic Plastic 24210-08, Electron Microscopy Sciences, Hatfield, PA 19440, USA) was cast and cured in a mold for 24 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Ycf1 sample was applied to a CFlat-1.2/1.3-4C-T (WT Ycf1) or QF-1.2/1.3-4Au grid (E1435Q Ycf1) purchased from Electron Microscopy Sciences. Grids were placed inside of a Leica EM GP2 equilibrated to 10°C and 80% humidity ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample (0.5 mg/ml) were applied to freshly glow-discharged (see above) C-flat 2/1-3C-T grids (Electron Microscopy Sciences) and plunged into liquid ethane after 2 s blot time with relative blot force set to 21 ...
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bioRxiv - Plant Biology 2021Quote: ... The roots of 7 to 10 day old seedlings were collected and fixed for 30 min with 2-(N-morpholino) ethane sulfonic acid (MES) buffer solution pH 5.7 containing 4% paraformaldehyde (32% methanol-free solution; Electron microscopy Sciences). pH was adjusted to 5.7 with KOH solution.
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bioRxiv - Bioengineering 2023Quote: ... 4 µl of AAV9 capsids containing GFP or Cas9 cargo was deposited on 2/2 400 mesh C-flat grids (Electron Microscopy Sciences, Hatfield, PA, #CF224C-50), which were previously glow discharged at 15 mA for 15 s on PELCO easyGLOW instrument ...
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bioRxiv - Bioengineering 2020Quote: Both the 3D bioprinted and the manually prepared SK-N-BE(2) neuroblastoma spheroids were fixed in 4% paraformaldehyde (PFA, Electron Microscopy Sciences) diluted in 1% Triton (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were coverslipped with mounting medium (EMS Immuno Mount DAPI and DABSCO, Electron Microscopy Sciences, Cat # 17989-98, Lot 180418). For optogenetics animals ...
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bioRxiv - Cell Biology 2021Quote: ... Washing and staining was performed by preparing a piece of parafilm with two 15-μL droplets of 1× folding buffer and two 15-μL droplets of 2% aqueous uranyl formate (electron microscopy sciences (EMS)) stain solution ...
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bioRxiv - Cell Biology 2022Quote: ... Glutaraldehyde (Electron Microscopy Science, cat n°16220). The following primary antibodies were used in this study ...
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bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2019Quote: ... fixed in 2 % PFA and allowed to attach on poly-L-lysine-coated 12 mm glass coverslips (Electron Microscopy Sciences, Hatfield, Pennsylvania) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: 10 μl of concentrated Pseudotyped SARS-CoV-2 S lentiviral particles were incubated for 30 min on carbon coated grids (EMS #CF200-CU). Excess solution was removed by back blotting ...
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bioRxiv - Neuroscience 2023Quote: ... and then coverslipped with mounting medium including a nuclear stain (EMS Immuno Mount DAPI and DABSCO, Electron Microscopy Sciences, Cat # 17989-98, Lot 180418). Coverslip edges were sealed with transparent nail polish ...
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bioRxiv - Immunology 2021Quote: ... 48-hour activated T cells from PRMT5fl/fl and T-PRMT5Δ/Δ mice were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, catalog no. 15713) for 10 min in V-bottom plates (Costar ...
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bioRxiv - Neuroscience 2023Quote: ... Stained or unstained slides were then dried and coverslipped with a mounting medium (EMS Immuno Mount DAPI and DABSCO, Electron Microscopy Sciences, 17989-98, lot 180418). After at least 12 H of drying ...
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bioRxiv - Neuroscience 2023Quote: ... including Goat-anti-Human IgG (H&L) and Goat-anti-Mouse IgG (H&L) (Electron Microscopy Sciences, Hatfield, PA) (1:20 dilution in 1% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Cat N° SND75 (microgroove length: 75 μm) and Cat N° XC150 (microgroove length: 150 μm)) and 7.8mil Aclar 33C sheets (Electron Microscopy Sciences, Hatfield, PA, USA, Cat N° 50425-25) were sterilized in 70% ethanol and left to air-dry in the laminar flow ...
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bioRxiv - Cell Biology 2022Quote: ... poly-L-lysine coated glass coverslips (#1.5, EMS) were placed into tissue culture treated 12-well plates and washed twice with growth medium and cells were seeded at 1.5 x 105 per well ...
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bioRxiv - Genetics 2022Quote: ... Then C→T or G→A substitutions which are usually induced by EMS mutagenesis ...
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bioRxiv - Cell Biology 2022Quote: ... mouse eyeballs were fixed o/n in 4% (w/v) PFA (171010; EMS) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... After washing with TBS-T and staining with Uranyless (Cat# 22409, Electron Microscopy Sciences), TEM imaging was conducted at the Biotron Experimental Research Center ...
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bioRxiv - Cancer Biology 2020Quote: HEKRON/EGFR cells were plated onto glow-discharged (EMS 150 T ES, Quorum Technologies), PLL coated glass coverslips overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated 2 min on 2% paraformaldehyde (Electron Microscopy Sciences), then incubated for 30 sec on 2% uranyl acetate (aqueous ...
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bioRxiv - Immunology 2019Quote: ... prior to deposition onto 2/2 Quantifoil grids (EMS) that were glow discharged for 10 s directly preceding the deposition in a Vitrobot (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... washed and fixed O/N in 4% paraformaldehyde/phosphate buffered saline (PBS) solution (EMS). Left lung lobes were then treated for paraffin inclusion ...
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bioRxiv - Cell Biology 2021Quote: ... myoblasts were plated on poly-L-lysine coated coverslips (EMS) at 70% confluency and cultured overnight.
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bioRxiv - Neuroscience 2023Quote: ... or a poly-L- lysine coated coverslip (Electron Microscopy Sciences), are fed twice weekly with 1mL neurobasal-A media supplemented with 500μM Glutamax ...
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bioRxiv - Biophysics 2022Quote: ... CF-2/2-4C (CF-224C-100) (Electron Microscopy Sciences) cryoEM grids ...
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bioRxiv - Neuroscience 2021Quote: ... 2% paraformaldehyde (EMS) in 0.1 M sodium cacodylate buffer (EMS) ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS), 2.2mM CaCl2 diluted in 50 mM MOPS buffer (pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: Cells cultured on coverslips were fixed in 4% PFA (Electron Microscopy Sciences, Cat. N. 15710) in PBS for 10 min followed by incubation in blocking solution for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... cells were fixed with PFA 4% in PBS 1X (Electron Microscopy Sciences, cat n°15700) for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... then for G/C to A/T transitions that were likely to be caused by EMS, and thirdly for non-synonymous amino-acid changes.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed again with PBS-T and mounted in Fluoro Gel with DABCO™ (Electron Microscopy Science) before visualizing with either a Leica DM2500 microscope equipped with a Leica DFC 7000T camera and a LAS X software or with a Zeiss 710 confocal microscope with Zen software (Zeiss) ...
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bioRxiv - Biochemistry 2024Quote: Quanifoil R 2/2 200 mesh copper grids (EMS #Q2100-CR2) were glow discharged at 25mA for 25s using a Pelco easiGlow system (Ted Pella) ...
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bioRxiv - Cell Biology 2019Quote: ... and 1:10 Goat-Anti-Mouse IgG (H+L) (EMS 25133) antibodies coupled with 6 nm and 15 nm gold particles ...
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bioRxiv - Bioengineering 2022Quote: ... Both channels (n=3 per donor) were fixed using 4% paraformaldehyde (PFA) solution (Electron Microscopy Sciences). The PFA solution was pipetted into both channels followed by a 15-minute incubation at room temperature (RT) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We stained them en bloc with 1% aqueous uranyl acetate (Electron Microscopy Sciences, cat n. 22400) for 16 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... A small piece (∼2 × 2 mm) containing V1 was cut out and prepared for EM by staining sequentially with 2% osmium tetroxide (EMS) in Sodium Cacodylate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Bioengineering 2022Quote: Degassed 2/2-3C C-flat grids (Electron Microscopy Sciences, Hatfield, PA) were glow discharged for 45 s at 30 mA ...
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bioRxiv - Biophysics 2024Quote: ... Quantifoil R 2/2 copper 200 mesh grids (Electron Microscopy Sciences #Q2100CR2) were glow-discharged for 45 seconds at 15 mA using a PELCO easiGLOW Glow Discharge System to apply a negative charge to their surface ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS, USA), 0.2% picric acid (EMS ...
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bioRxiv - Neuroscience 2021Quote: ... 2% glutaraldehyde (EMS 16000), p.H 7.4 ...