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Citations for Electron Microscopy Sciences :
1 - 50 of 252 citations for Chikungunya Virus VLP E1 E2 C protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the μl of virus solution were placed on glow discharged C-Flat 2/2 grids (EMS) and plunge frozen into liquid ethane using a Gatan Cryoplunge 3 ...
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bioRxiv - Biophysics 2019Quote: 5 uL of pCV2-2 VLP samples was applied to CF200-CU carbon film 200 mesh copper grids (Electron Microscopy Science) for 1 min and the grids were then washed with 200 µl of 50 mM of Na Cacodylate buffer and then strained immediately with 50 µl of 0.5% uranyl acetate for 1 min ...
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bioRxiv - Bioengineering 2022Quote: ... VLP samples were fixed with 2% (v/v) glutaraldehyde in water solution on 400 mesh Formvar-coated copper grids (EMS Cat#FF400-Cu). After fixation ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 1 mg/ml (final for protein) and applied to freshly glow-discharged C-flat 300 mesh copper grids (CF-1.2/1.3, Electron Microscopy Sciences) at 4℃ ...
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bioRxiv - Microbiology 2020Quote: ... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
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bioRxiv - Microbiology 2019Quote: ... Mock or virus-infected cells were fixed in 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, USA) for 30 min and permeabilized with 0.1% Triton X (Fisher Bioreagents ...
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bioRxiv - Microbiology 2023Quote: ... 3 µL of the purified CVB5 mutant virus sample at 4.5 mg mL-1 concentration was loaded onto Quantifoil R 1.2/1.3 grids (EMS), that were previously glow-discharged for 30 s in a GloCube Plus device (Quorum Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... and 1 μL of virus mixed with Fast Green FCF Solution 2.5% (Electron Microscopy Sciences, Hatfield, PA, USA). The mouse was placed on a heating blanket (37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and filled with 0.5 µL of virus (titer ∼ 1012) dyed with Fast Green 2.5% (Electron Microscopy Sciences, Hatfield, PA, U.S.A.). The mice (∼8 weeks old ...
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bioRxiv - Microbiology 2020Quote: Four microliters of purified virus were applied to a Formvar-and carbon-coated 400-mesh copper grid (Electron Microscopy Sciences, Hatfield, PA) for 25 seconds ...
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bioRxiv - Microbiology 2021Quote: Four μL of purified virus was applied to a Formvar- and carbon-coated 400-mesh copper grid (Electron Microscopy Sciences, Hatfield, PA) for 25 seconds ...
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bioRxiv - Microbiology 2021Quote: ... approximately 4 μL of purified virus sample was applied to a glow-discharged 300-mesh copper grid coated with continuous carbon film (Electron Microscopy Sciences, Hatfield, PA). The grid was plunged into a liquid ethane container that is further cooled by a liquid nitrogen bath ...
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bioRxiv - Biophysics 2023Quote: U2OS cells grown in #1.5H Ibidi chambers (µ-Slide 8 well Cat#80826) at 37°C in 5% CO2 airgas were fixed in 37°C 3% paraformaldehyde (EMS) and 0.1% glutaraldehyde (EMS ...
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bioRxiv - Biochemistry 2021Quote: ... Quantifoil and C-flat grids (Electron Microscopy Sciences) were glow discharged with Pelco easiGlow for 60s at 25mA (negative polarity) ...
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bioRxiv - Immunology 2022Quote: ... 1.2/1.3 C-Flat grid (Electron Microscopy Sciences). After 20 s of incubation ...
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bioRxiv - Neuroscience 2021Quote: ... and perfused transcardially with warm (~37 °C) Ames medium (MilliporeSigma) with heparin (MilliporeSigma) followed by warm (~37 °C) 2.5% glutaraldehyde (Electron Microscopy Sciences), 2% paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4 °C in 4% paraformaldehyde (PFA) (EMS, 15710). Then they were washed with PBS-Tween20 (0.1% ...
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bioRxiv - Biophysics 2021Quote: ... C-flat holey carbon grids (CF-1.2/1.3-4Au, EMS) were glow-discharged for 20 s prior to the application of 3.5 μL of sample ...
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bioRxiv - Biophysics 2022Quote: ... C-flat holey carbon grids (CF-1.2/1.3-4Au, EMS) were glow-discharged for 20 seconds prior to the application of 3.5 μL of sample ...
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bioRxiv - Biophysics 2022Quote: ... C-flat holey carbon 1.2/1.3 grids (Electron Microscopy Sciences) or UltrAuFoil holey gold film grids (Ted Pella ...
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bioRxiv - Neuroscience 2022Quote: Cells were fixed with 37°C 4% paraformaldehyde (Electron Microscopy Sciences) in 1X PBS for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... or -20°C methanol with 1% paraformaldehyde (Electron Microscopy Sciences, 15710) for 10 minutes on ice ...
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bioRxiv - Cell Biology 2019Quote: ... 15 nm protein A-coated gold beads (EMS) were adhered to the grids prior to Reynolds’ lead citrate staining ...
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bioRxiv - Cell Biology 2021Quote: ... and fixed overnight at 4°C with 4% Paraformaldehyde (PFA) (EMS #15710). Specimens (roughly 3-cm pieces ...
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bioRxiv - Biophysics 2022Quote: C-flat™ EM (CF-2/1-4C-T, Electron Microscopy Sciences) grids were glow-discharged for 15 seconds on a Pelco Easiglow at 15mW power ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C and fixed in suspension in 4% paraformaldehyde (EMS 15714) with 0.0075% glutaraldehyde (EMS 16220 ...
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bioRxiv - Microbiology 2023Quote: ... and embedded progressively at -20°C in LR Gold resin (EMS, USA). Resin polymerization was carried out at -20°C for 7 days under UV illumination ...
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bioRxiv - Bioengineering 2022Quote: Degassed 2/2-3C C-flat grids (Electron Microscopy Sciences, Hatfield, PA) were glow discharged for 45 s at 30 mA ...
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bioRxiv - Molecular Biology 2023Quote: ... 3µL of protein was applied to Quantifoil grids (EMS) that were glow-discharged for 30s at 15 mA ...
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bioRxiv - Microbiology 2021Quote: ... Next, baked (60°C, overnight) CF300-Cu-UL EM grids (Electron Microscopy Sciences) were placed on top of the wells with the carbon side facing downwards ...
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bioRxiv - Biophysics 2022Quote: C-Flat 2/2-3C or 1.2/1.3-3C grids (Electron Microscopy Sciences) were glow discharged at 10 mA for 90 seconds ...
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bioRxiv - Immunology 2019Quote: ... samples were incubated overnight at 4°C in Uranyl Acetate Replacement (UA, EMS) in H2O (1:3) ...
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bioRxiv - Neuroscience 2021Quote: ... slices were stored overnight at 4°C in 4% PFA (Electron Microscopy science) and moved to phosphate-buffered saline (PBS ...
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bioRxiv - Genetics 2022Quote: ... Then C→T or G→A substitutions which are usually induced by EMS mutagenesis ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were fixed in warm (37°C) 3% paraformaldehyde (PFA, Electron Microscopy Sciences) 2% sucrose ...
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bioRxiv - Neuroscience 2023Quote: ... the EV preparations were fixed overnight at 4°C in 2% PFA (EMS) and 5μL of sample applied to formvar coated gold grids (EMS ...
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bioRxiv - Biophysics 2024Quote: ... C-flat 1.2/1.3 holey carbon Au 300 mesh grid (Electron Microscopy Sciences) in a Leica EM GP plunge freezer operating at 25 °C and 100% humidity ...
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bioRxiv - Biophysics 2021Quote: ... at 37°C and then treated with fixing buffer (3% Paraformaldehyde (Electron Microscopy Sciences), 0.1% Glutaraldehyde (Electron Microscopy Sciences) ...
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bioRxiv - Developmental Biology 2019Quote: ... then fixed 3h at 4°C in 4% paraformaldehyde (PFA, Electron Microscopy Sciences, 15710), 0.5% Triton X-100 (SIGMA ...
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bioRxiv - Cell Biology 2019Quote: ... The diluted sample was applied to C-flat holey carbon grids (Electron Microscopy Sciences, CF-1.2/1.3-3C for screening ...
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bioRxiv - Bioengineering 2020Quote: ... at 4°C with 50-50 acetone-Epon812 epoxy resin (Electron Microscopy Sciences, 14120). Next day ...
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bioRxiv - Cell Biology 2021Quote: ... were first fixed with 37 °C pre-warmed 3% PFA (15710, Electron Microscopy Sciences) and 0.5% GA (16019 ...
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bioRxiv - Biochemistry 2020Quote: C-flat Holey Carbon grids with gold support (CF-1.2/1.3-3Au-50, EMS) were made hydrophilic by glow-discharging at 30 mA for 30 s using PELCO easiGlow (Ted Pella) ...
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bioRxiv - Immunology 2020Quote: ... at 4°C and post-fixed with 1% osmium tetroxide (Electron Microscopy Sciences, UK) and 1,5 % ferrocyanide (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... washed twice in PBS (23°C) and fixed in either 1% glutaraldehyde (EMS, 16020) prepared in BRB80 buffer (80 mM PIPES pH 6.9 ...
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bioRxiv - Genetics 2020Quote: ... submerged in TFM (Cat. TFM-C) within a disposable embedding mold (Cat. EMS 70183). Human Visp cortex samples were provided by the Oregon Brain Bank 5.5 hours post-mortem and were submerged in TFM ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then serially dehydrated at 4 °C and embedded in EmBed812 (EMS, 14120). 80 nm sections were taken using an UC7 (Leica ...
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bioRxiv - Biochemistry 2023Quote: ... and placed in an acrylic matrix (Electron Microscopy Sciences, 69080-C or 69080-S) to make either coronal or sagittal slices (1 mm thickness) ...
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bioRxiv - Neuroscience 2023Quote: ... at −20°C using biopsy pens EMS Rapid-Core (Electron Microscopy Sciences, Hatfield, USA). To localize the subregions of interest in the brain ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were cleaned by standard acetone precipitation (EM grade acetone; EMS). The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich ...