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Citations for Electron Microscopy Sciences :
1 - 50 of 743 citations for 7 Phenylsulfanyl bicyclo 3.2.0 hept 2 en 6 one racemic since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... A standard rOTO en bloc staining protocol39 was applied including postfixation in 2% osmium tetroxide (EMS), 1.5% potassium ferricyanide (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were mounted on microscope slides with mounting medium containing 1,4-diaza-bicyclo[2.2.2]octane (Electron Microscopy Sciences, Hatfield, PA). Fluorescence images were taken using the Olympus optical elements fluorescence microscope (Olympus) ...
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bioRxiv - Molecular Biology 2022Quote: ... coverslips were mounted on microscope slides with mounting medium containing 1,4-diaza-bicyclo[2.2.2]octane (Electron Microscopy Sciences, Hatfield, PA). Fluorescence images were taken using the Olympus optical elements fluorescence microscope (Olympus Optical Co. ...
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bioRxiv - Cell Biology 2021Quote: ... and rinsed in distilled water prior to en bloc staining with 2% uranyl acetate (22400, Electron Microscopy Sciences, USA). After dehydration through a graded ethanol series ...
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bioRxiv - Biophysics 2021Quote: ... and Quantifoil carbon grids with 7 µm holes (S 7/2, Electron Microscopy Sciences) were cleaned with 100% chloroform to remove the plastic cover ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: Eye cups from mice aged 4-8 months (Figures 2–5) or 10 days postnatal (Figures 6, 7, 9) were fixed for five minutes in 1% PFA (Electron Microscopy Science) diluted in 1x PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and coverslips were mounted on microscope slides with mounting media containing 1,4-diaza-bicyclo[2.2.2]octane (Electron Microscopy Sciences, Hatfield, PA). Labeled cells were visualized by epifluorescence with an Olympus BX60 Fluorescence Microscope (OPELCO ...
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bioRxiv - Neuroscience 2023Quote: ... We applied a standard reduced osmium thiocarbohydrazide osmium (rOTO) en bloc staining protocol (Kislinger et al., 2020a) including postfixation in 2% osmium tetroxide (EMS), 1.5% potassium ferrocyanide (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... 0.1% uranyl acetate and 2% H2O followed by en bloc Osmium-Thiocarbohydrazide-Osmium staining75,76 and embedding in Durcupan ACM (Electron Microscopy Sciences). Serial block-face imaging was conducted in a Zeiss Sigma 3View system with variable pressure with 30 nm x 70 nm resolution at 5.5 kV ...
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bioRxiv - Cell Biology 2021Quote: ... before en bloc staining with 1% uranyl acetate (Electron Microscopy Sciences) in water for 1 hour at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... En Bloc staining in 1% uranyl acetate (Electron Microscopy Sciences, Hartfield, PA) was used to increase the contrast ...
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bioRxiv - Microbiology 2022Quote: ... samples were en bloc stained in 1% aqueous uranyl acetate (Electron Microscopy Sciences, Hatfield, PA) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... the en bloc staining was carried out overnight with 1% uranyl acetate (Electron Microscopy Sciences) at 4 °C followed by 3 washes in water ...
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bioRxiv - Neuroscience 2023Quote: ... Contrast was enhanced by en bloc staining with 0.5% uranyl acetate (22400, Electron Microscopy Sciences) before dehydration in ethanol and embedding in Embed 812 (14120 ...
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bioRxiv - Cell Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (Electron Microscopy Sciences, #17985-10) and viewed by confocal microscope (Leica SP8 ...
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bioRxiv - Immunology 2023Quote: ... Negative staining was performed for one minute using 2% Uranyl Acetate (EMS). The phage and bound antibody structures were visualized using JEM-1400 (Jeol ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated en-bloc in Walton’s lead aspartate solution (0.066 gr lead nitrate (#17900 EMS) dissolved in 10 ml of 0.003 M aspartic acid solution ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We stained them en bloc with 1% aqueous uranyl acetate (Electron Microscopy Sciences, cat n. 22400) for 16 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were further fixed for 7 days in 2% glutaraldehyde (Electron Microscopy Sciences: 16320)/ 4% paraformaldehyde/ 0.1M sodium-cacodylate at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 6-diamidino-2-phenylin dole (DAPI) (17985-50, Electron Microscopy Sciences). Cells visualized and photographed by fluorescence microscopy (Nikon ...
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bioRxiv - Immunology 2022Quote: ... The sample was stained by 6 µL 2% uranyl acetate solution (Electron Microscopy Sciences, Catalog number 22400-2) twice ...
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bioRxiv - Cell Biology 2024Quote: ... tissue was rinsed in 0.1N acetate buffer (pH 5.0-5.2) at room temperature and then en bloc stained overnight with 1% uranyl acetate (EMS) in acetate buffer at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and post-fixed for one hour with 2% osmium tetroxide (Electron Microscopy Science, USA, cat. 19190) in 0.15 M of phosphate buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... mounted using DAPI (4ʹ,6- diamidino-2-phenylindol) Fluoromount G mounting medium (Electron Microscopy Science) and imaged with an Axio Observer microscope (Zeiss).
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bioRxiv - Genetics 2020Quote: ... sections were mounted with Fluoromount-G containing 4′,6-diamidino-2-phenylindole (DAPI) (Electron Microscopy Sciences). Staining was visualized and imaged using a Nikon 80i microscope with a Nikon DS-Fi1 camera or a Nikon Eclipse TiE attached to an A1R-SHR confocal ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; 17985-50; Electron Microscopy Sciences, Hatfield, PA, USA). Images were taken with an All-in-One Fluorescence Microscope BZ-X710 (KEYENCE Corp. ...
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bioRxiv - Developmental Biology 2019Quote: ... the larval fish and adults with exposed brains were further fixed for 7-14 days in 2% glutaraldehyde (Electron Microscopy Sciences: 16320)/ 4% paraformaldehyde/ 0.1M sodium-cacodylate at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Plant Biology 2023Quote: ... drd1-6 (EMS)55 ...
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bioRxiv - Immunology 2022Quote: ... The sample was double-stained with 6 µl of 2% (w/v) uranyl formate (Electron Microscopy Sciences, catalog number 22450) for 5 seconds (first stain ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3–4 times 10 min each with fresh acetone chilled to −90°C and left at the same temperature for 6 hours in acetone containing 2% osmium (Science services (EMS)) and 0.2% uranyl acetate (Serva) ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were incubated with Aurion blocking solution for one hour and washed 2 × 15 mins with incubation solution (Electron microscopy sciences, Cat # 25558). Then the slices were incubated with goat anti-BirA in incubation solution (1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... isoflurane overdose was used to deeply anesthetize mice and cold PBS was transcardially perfused for 2 minutes followed by 7 minutes of perfusion with 4%PFA freshly made from 32% (Electron Microscopy Sciences Cat #15714-S) at a rate of 7 mL/min ...
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bioRxiv - Neuroscience 2021Quote: ... the grid was stained one time for 3 minutes and three times for 1-minute each using 2% uranyl acetate (Electron Microscopy Sciences, Hatfield, PA). Excess stain was wicked away in between the steps ...
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bioRxiv - Plant Biology 2022Quote: ... one volume of 0.5X AGM was mixed with one volume of 8% Paraformaldehyde (PFA, Electron Microscopy Sciences cat# 15700) in the corresponding buffer for a final concentration of 4% PFA ...
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bioRxiv - Genomics 2023Quote: ... and the cmt3-7 (N6365) and recq4a-W387X (EMS) [64] mutants are in the Ler accession [59] ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) and coverslips were mounted over slides in Fluoro-Gel (EMS; 17985-10) and imaged by spinning disc confocal microscopy and Aperio Versa 8 fluorescent slide scanner.
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bioRxiv - Cancer Biology 2020Quote: ... ∼7 ×107 cells were fixed in 1% formaldehyde (EMS 50980485) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated in 4’,6-diamidino-2-phenylindole (DAPI) and mounted onto slides with Fluoro-Gel fluorescence mounting medium (Electron Microscopy Sciences Cat# 1798502). Expression was observed via confocal microscopy.
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bioRxiv - Neuroscience 2021Quote: ... One 4mm cover glass (round, #1.5 thickness, Electron Microscopy Sciences) was adhered to the bottom of a custom titanium insert and placed onto the brain to gently compress the underlying cortex and dampen biological motion during imaging ...
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bioRxiv - Genetics 2023Quote: ... pH 6 (64142-07, Electron Microscopy Sciences). The tissue sections were then incubated in the Click-iT Edu Reaction Cocktail for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... One 4 mm cover glass (round, #1.5 thickness, Electron Microscopy Sciences) was adhered to the bottom of a custom titanium insert and placed onto the brain to gently compress the underlying cortex and dampen biological motion during imaging ...
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bioRxiv - Neuroscience 2021Quote: ... and 1.5% K3Fe(CN)6 (EMS, Hatfield, USA) at 4°C followed by a contrasting step with 1% thiocarbohydrazide (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... one half was post-fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) in PBS for 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... or 7 days prior to intracardiac perfusion with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, 15714) in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Flowers older than stage 7 were dissected using Dumont tweezers (Electron Microscopy Sciences, Style 5) under a stereo microscope (ZEISS Stemi 2000) ...
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bioRxiv - Plant Biology 2023Quote: ∼6 000 seeds were mutagenized using 0.4 % ethyl methanesulfonate (EMS) in 100 mM phosphate buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated 2 min on 2% paraformaldehyde (Electron Microscopy Sciences), then incubated for 30 sec on 2% uranyl acetate (aqueous ...
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bioRxiv - Immunology 2019Quote: ... prior to deposition onto 2/2 Quantifoil grids (EMS) that were glow discharged for 10 s directly preceding the deposition in a Vitrobot (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and placed on a microscope slide with one drop of Citifluor (Electron Microscopy Sciences). Slides were visualized with a Zeiss Axio Observer D1 confocal epifluorescence microscope using a DAPI filter (Zeiss filter set 49) ...