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Citations for Electron Microscopy Sciences :
1 - 50 of 1783 citations for 6 amino m xylene 4 sulphonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... slides were immersed three times (4 min) in xylene and mounted on coverslips with Entellam (Electron Microscopy Sciences). Images were taken with a 63x objective in a white field ...
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bioRxiv - Cell Biology 2023Quote: Samples fixed 45 m in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS were stained 20 m with 1μg/mL Hoechst (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then kept in Xylene (23400, Electron Microscopy Sciences) before mounting using permount (SP15-500 ...
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bioRxiv - Cell Biology 2020Quote: ... Fixation was performed in 0.1 M PBS containing 4% paraformaldehyde (Electron Microscopy Sciences) for 2-3 hours on ice with mild agitation ...
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bioRxiv - Bioengineering 2022Quote: ... with 4% PFA in 0.1 M Na Cacodylate buffer (Electron Microscopy Sciences, 11652)) at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The output list of CandiSNP was screened for nonsynonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a special focus on the chromosome with the highest SNP density with an allele frequency of 1 ...
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bioRxiv - Plant Biology 2023Quote: ... The output list of CandiSNP was screened for non-synonymous amino acid changes and the G/C to A/T transitions that were likely to be caused by EMS, with a particular focus on the chromosome with the highest density of SNPs with an allele frequency of >0.75.
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bioRxiv - Genomics 2020Quote: ... cleared with xylene and mounted with Permount Mounting Medium (Electron Microscopy Sciences)36 ...
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bioRxiv - Neuroscience 2023Quote: ... they were mounted with xylene-based mounting medium (Entellan, Electron Microscopy Sciences). Images were captured with a bright-field microscope (Nikon Eclipse 80i ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were fixed overnight at 4°C in freshly prepared phosphate buffer (0.1 M, pH 7.4) containing 4% paraformaldehyde (15700, Electron Microscopy Sciences) and 0.2% glutaraldehyde (16200 ...
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bioRxiv - Cell Biology 2019Quote: ... 8.5 µm, 6 µm, 6.5 µm, 6.5 µm, 6.5 µm for P0, AB, P1, ABa, ABp and EMS, respectively ...
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bioRxiv - Neuroscience 2020Quote: ... cleared in xylene (2×5 minutes) and embedded in Entellan (EMS, Hatfield, PA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... dehydration to xylene and finally cover slipping with DPX (Electron Microscopy Sciences, #13512). All steps were performed at room temperature unless otherwise indicated ...
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bioRxiv - Genetics 2023Quote: ... Nucleic acid and protein staining were performed using 4% solutions of Uranyl Acetate (UA) (Electron Microscopy Sciences cat. 22400-4) and Phosphotungstic acid hydrate (PTA ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared in xylene and coverslipped using Cytoseal 60 (Electron Microscopy Sciences, 18007).
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bioRxiv - Bioengineering 2023Quote: ... 4% Paraformaldehyde in 0.1 M phosphate buffer (15735-50S) was purchased from Electron Microscopy Sciences (PA). Ethanol absolute (64-17-5 ...
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bioRxiv - Bioengineering 2023Quote: ... followed by pre-treatment with 6% nitric acid and sequential staining with 0.1% toluidine blue (EMS #26074-15) and picrosirius red (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... incubated in Xylene 2 × 10 min and embedded in DPX (Electron Microscopy Sciences; # 180627-05) and incubated for at least 4 days ...
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bioRxiv - Cell Biology 2019Quote: Animals were transcardially perfused using 4% paraformaldehyde in 0.1 M sodium cacodylate (Electron Microscopy Sciences, Hatfield, PA) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were transcardially perfused initially with 0.1 M PBS followed by 4% paraformaldehyde (PFA; Electron Microscopy Sciences) in 0.1 M PBS solution ...
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bioRxiv - Neuroscience 2021Quote: ... the coverslips were mounted in dibutyl phthalate in xylene (DPX, #13512, Electron Microscopy Sciences, Hatfield, Pennsylvania) with the tissue facing down on a microscope slide with spacers ...
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bioRxiv - Biophysics 2021Quote: ... at a final concentration of 2.5% at room temperature for 10 minutes followed by and an overnight fixation with Karnovsky’s fixative reagent (2.5% glutaraldehyde, 2% paraformaldehyde, and 0.1% tannic acid prepared in 0.1 M phosphate buffer, pH 7, 15720, Electron Microscopy Sciences, Inc., PA). We washed the cells once with 0.1 M pH 7.2 phosphate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... IP) mice were paraformaldehyde-fixed (4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4, EMS Diasum, Hatfield, PA) through transcardial perfusion ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 M glucose) and placed on 6 mm diameter chambers made from a silicon sheet using a core sampling tool (EMS # 69039-60). The silicon chamber was mounted on a 1.5 mm clean coverglass (EMS # 72200-31 ...
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bioRxiv - Neuroscience 2024Quote: ... Unbound antibodies were removed using washing buffer (0.1% BSA in 0.1 M PB) and grids were incubated with 6 nm gold-conjugated donkey-anti-rabbit IgG (1:25, #25104, Electron Microscopy Sciences) for 20 min.
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bioRxiv - Genetics 2020Quote: ... sections were mounted with Fluoromount-G containing 4′,6-diamidino-2-phenylindole (DAPI) (Electron Microscopy Sciences). Staining was visualized and imaged using a Nikon 80i microscope with a Nikon DS-Fi1 camera or a Nikon Eclipse TiE attached to an A1R-SHR confocal ...
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bioRxiv - Neuroscience 2019Quote: ... then cleared in xylene and coverslipped with DPX mounting media (Electron Microscopy Sciences, Hatfield, PA; cat# 13512).
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bioRxiv - Neuroscience 2023Quote: ... Sections were cleared with xylenes for 5 minutes and coverslipped with DPX (Electron Microscopy Sciences, Hatfield, PA) mounting medium ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were allowed to grow for 6 h and then compressed for 18 h before being fixed with 4% paraformaldehyde (# 30525-89-4, Electron Microscopy Sciences, Hatfield, PA). The non-invasive cells on the upper chamber were removed with cotton swabs ...
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bioRxiv - Developmental Biology 2019Quote: ... gmIs18 or vab-8(e1017)gmIs18 worms were mutagenized for 4 hours by incubation in 0.05 M ethyl methane sulfonate (EMS). Worms were washed in M9 buffer (22mM KH2P04 ...
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bioRxiv - Cell Biology 2020Quote: N2 worms were mutagenized for 4 h by incubation in the presence of 0.05 M ethyl methane sulfonate (EMS) according to the standard protocol (24) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with freshly prepared and filtered 1% tannic acid (TA, CAS#1401-55-4, EMS) in water using a Pelco BioWave microwave for seven 1-minute cycles alternating between 150 W and 0 W power ...
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bioRxiv - Cancer Biology 2021Quote: Human or mouse FFPE tumor tissue sections were deparaffinized in xylene alternative (Histo-Clear, EMS; 3×5 min) and rehydrated in EtOH/H2O gradient series (100% ...
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bioRxiv - Neuroscience 2022Quote: ... slides were dehydrated through xylene and cover-slipped using DPX mounting media (Electron microscopy systems [EMS], Cat. 13510).
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bioRxiv - Neuroscience 2023Quote: Mice (female; n=4) at 15 dpl were perfused with PFA prepared in 0.1 M Sorensen’s buffer (Electron Microscopy Sciences). The spinal cords were harvested ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected brains were moved through 6 wells (12ηl/well) containing a fixation solution (4% paraformaldehyde, Electron microscopy sciences #15713 in PBT (0.3% Triton in PBSX1 ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; 17985-50; Electron Microscopy Sciences, Hatfield, PA, USA). Images were taken with an All-in-One Fluorescence Microscope BZ-X710 (KEYENCE Corp. ...
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bioRxiv - Genomics 2019Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Biophysics 2020Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences. 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Plant Biology 2021Quote: Marchantia thalli were cut in small pieces (1×2 mm) and fixed immediately in buffered phosphate 0,1 M paraformaldehyde 4% (Electron Microscopy Sciences), glutaraldehyde 2,5% (TAAB Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... CHO or HeLa cells were fixed for 30 minutes with 4% paraformaldehyde in 0.1 M PBS (15735-85; Electron Microscopy Sciences) on cover glasses coated in poly-L-lysine (P4832 ...
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bioRxiv - Neuroscience 2023Quote: ... and perfused intracardially with cold saline followed by ∼40 mL 4% PFA in 0.01 M phosphate buffered saline (PBS) (freshly diluted from 32% EM Grade PFA; Electron Microscopy Science). The brains were dissected out and post-fixed in 4% PFA at 4 °C for 24 hr ...
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bioRxiv - Biophysics 2023Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
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bioRxiv - Cancer Biology 2023Quote: ... MFPs were fixed in cold 2% v/v glutaraldehyde with 4% paraformaldehyde in 0.1 M sodium cacodylate buffer (Electron Microscopy Sciences) for 4 hr followed by one wash in the same buffer ...
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Exploring the Role of Spatial Confinement in Immune Cell Recruitment and Regeneration of Skin WoundsbioRxiv - Bioengineering 2023Quote: ... they were stained in Phosphomolybdic Acid-Phosphotungstic Acid (EMS) for 15 minutes and then in Aniline Blue (EMS ...
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bioRxiv - Neuroscience 2023Quote: ... then taken through EtOH and Xylene dehydration and clearing steps and coverslipped using permount mounting medium (Electron Microscopy Science, PA). A Leica DMi8 automated microscope with Thunder 3D assay imaging software (Leica Microsystems ...
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bioRxiv - Neuroscience 2023Quote: ... all animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... animals were deeply anesthetized with 5% isoflurane in 100% oxygen and perfused through the ascending aorta with 20 ml of phosphate buffered saline (PB; pH 7.4) followed by 4% phosphate-buffered (0.1 M; pH 7.4; 20 ml) paraformaldehyde (Electron Microscopy Sciences, Fort Washington, PA). The brains were removed and stored in the perfusion fixative for 4 h at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... They were quickly soaked in xylene and mounted on microscope slides with Permount Mounting Medium (Electron Microscopy Science, Hatfield, PA, USA). Images were captured with a stereo microscope SMZ1270 (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was perfused quickly with cold 0.1 M PBS to clear blood before fixing the brain with cold 4% paraformaldehyde (PFA, Electron Microscopy Sciences, Hatfield, USA). Brains were post fixed in 4% PFA solution overnight (12-24 hr ...