Labshake search

1901 - 1950 of 7095 citations since 2019

• Isoform-specific targeting properties of the protocadherin CDHR5 control its apical delivery to promote brush border assembly

  Samaneh Matoo, et al., bioRxiv - Cell Biology 2023

  Quote: ... HeLa cells seeded on coverslips were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 min at RT ...
• Heat inactivation of foetal bovine serum causes protein contamination of extracellular vesicles

  Ornella Urzì, et al., bioRxiv - Cell Biology 2023

  Quote: ... 5 μg of EVs was placed onto glow discharged 200-mesh formvar/carbon copper grids (Electron Microscopy Sciences, Hatfield Township, PA). The EVs were washed two times with water and then fixed in 2.5% glutaraldehyde ...
• Reprogramming Intrahepatic Cholangiocarcinoma Immune Microenvironment by Chemotherapy and CTLA-4 Blockade Enhances Anti-PD1 Therapy

  Jiang Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Ruthenium tetroxide 0.5% Aqueous Solution (Electron Microscopy Sciences PN 20700-05) was used as a counterstain ...
• Integration host factor regulates colonization factors in the bee gut symbiont Frischella perrara

  K. Schmidt, et al., bioRxiv - Microbiology 2023

  Quote: ... Each bacteria suspension was adsorbed on a glow-discharged copper 400 mesh grid coated with carbon (EMS, Hatfield, PA, US) during one minute at room temperature ...
• Differentiation state and culture conditions impact neural stem/progenitor cell-derived extracellular vesicle bioactivity

  Dipankar Dutta, et al., bioRxiv - Bioengineering 2023

  Quote: An ultrathin carbon film grid (Electron Microscopy Sciences (Hatfield, PA)) was carefully floated on the droplet and allowed to incubate for 20min ...
• Abnormal chondrocyte intercalation in a zebrafish model of cblC syndrome restored by an MMACHC cobalamin binding mutant

  David Paz, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Embryos were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) overnight at 4°C ...
• Synthetic lipid rafts formed by cholesterol nano-patch induce T cell activation

  Yunmin Jung, et al., bioRxiv - Bioengineering 2023

  Quote: ... cells were fixed with 4 % (w/v) paraformaldehyde (PFA) (Electron Microscopy Sciences) in PBS for 15 min at Room temperature (RT ...
• Constitutively synergistic multiagent drug formulations targeting MERTK, FLT3, and BCL-2 for treatment of AML

  James M Kelvin, et al., bioRxiv - Bioengineering 2023

  Quote: ... liposomes were added to formvar/carbon coated copper grids (400 mesh; Electron Microscopy Sciences, Hatfield, PA) in ultrapure water solution ...
• Tissue-engineered models of lung cancer premalignancy

  Rachel Blomberg, et al., bioRxiv - Bioengineering 2023

  Quote: Hydrogel-embedded PCLS punches were fixed in 4% paraformaldehyde in PBS (Electron Microscopy Sciences) for 30 min ...
• Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanism

  Daniel Levy, et al., bioRxiv - Bioengineering 2023

  Quote: ... the grid was washed using a droplet of MilliQ water before being floating on a droplet of uranyl acetate replacement stain (22405; Electron Microscopy Sciences, Hatfield, PA, USA). Grids were then allowed to dry before storage and eventual imaging using a JEM 2100 LaB6 TEM (JEOL USA Incorporated ...
• Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanism

  Daniel Levy, et al., bioRxiv - Bioengineering 2023

  Quote: ... EVs were incubated with electron microscopy-grade paraformaldehyde (157-400-100; Electron Microscopy Sciences, Hatfield, PA, USA) before floating a carbon film grid (CF-200-Cu-25 ...
• Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanism

  Daniel Levy, et al., bioRxiv - Bioengineering 2023

  Quote: ... before floating a carbon film grid (CF-200-Cu-25; Electron Microscopy Sciences, Hatfield, PA, USA) on a droplet of the EV/PFA mixture ...
• The NLRP3 inflammasome selectively drives IL-1β secretion by Pseudomonas aeruginosa infected neutrophils and regulates bacterial killing in vivo

  Martin S. Minns, et al., bioRxiv - Microbiology 2023

  Quote: ... 500,000 neutrophils were seeded onto sterile 12mm circular glass coverslips (#1.5, Thermo or EMS) in 24-well plates and stimulated as indicated ...
• N-dihydrogalactochitosan reduces mortality in a lethal mouse model of SARS-CoV-2

  Christopher M. Weiss, et al., bioRxiv - Microbiology 2023

  Quote: ... The anterior half of the cranium was harvested as described[72] and placed into 4% paraformaldehyde (Cat. 157-8-100, Electron Microscopy Sciences) for 24 h at room temperature ...
• A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 is linked to neurodevelopmental disorders

  Abner Gonzalez, et al., bioRxiv - Biophysics 2023

  Quote: ... Cells were then fixed with 4% paraformaldehyde (#15710, Electron Microscopy Sciences), permeabilized with 0.5% Triton X-100 (#22140 ...
• A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 is linked to neurodevelopmental disorders

  Abner Gonzalez, et al., bioRxiv - Biophysics 2023

  Quote: ... followed by a rapid addition of glutaraldehyde (#16100, Electron Microscopy Sciences) to a final concentration of 0.025% for 1 minute ...
• A new Karyopherin-β2 binding PY-NLS epitope of HNRNPH2 is linked to neurodevelopmental disorders

  Abner Gonzalez, et al., bioRxiv - Biophysics 2023

  Quote: ... permeabilized with 0.5% Triton X-100 (#22140, Electron Microscopy Sciences), and blocked in 3% bovine serum albumin (# A8806 ...
• Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay

  Aparajita Dasgupta, et al., bioRxiv - Biophysics 2023

  Quote: ... cells were incubated with a 4% PFA + 0.1% glutaraldehyde (Electron Microscopy Sciences, 16020) solution made in PBS ...
• Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay

  Aparajita Dasgupta, et al., bioRxiv - Biophysics 2023

  Quote: ... cells were incubated with a 4% PFA solution made in PBS (Electron Microscopy Sciences, 15710, Hatfield, PA) for 15 min at room temperature and then washed three times (5 min each ...
• Cryo-EM reveals the mechanism of DNA compaction by Mycobacterium smegmatis Dps2

  Priyanka Garg, et al., bioRxiv - Biophysics 2023

  Quote: R 1.2/1.3 (Quantifoil) (Electron Microscopy Sciences) 300-mesh copper grids were glow-discharged for 90 s at 20 mA using Quorum GlowQube before sample preparation.3 μl of 0.1 mg/ml purified MsDps2 protein ...
• CRISPR/Cas9-mediated knockout of the ubiquitin variant Ub^KEKS reveals a role in regulating nucleolar structures and composition

  Julie Frion, et al., bioRxiv - Cell Biology 2023

  Quote: ... All reagents were purchased from Electron Microscopy Sciences (Cedarlane, Hornby, Canada).
• p53 modulates kinase inhibitor resistance and lineage plasticity in NF1-related MPNSTs

  Jamie L. Grit, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and blued in Scott’s Tap Water substitute (Electron Microscopy Sciences), and dehydrated through an ethanol gradient (70% ...
• Therapy Development for Microvillus Inclusion Disease using Patient-derived Enteroids

  Meri Kalashyan, et al., bioRxiv - Cell Biology 2023

  Quote: ... 2% formaldehyde (Electron Microscopy Sciences) in 0.1M Sodium Cacodylate (Sigma-Aldrich ...
• Therapy Development for Microvillus Inclusion Disease using Patient-derived Enteroids

  Meri Kalashyan, et al., bioRxiv - Cell Biology 2023

  Quote: ... followed by propylene oxide (Electron Microscopy Sciences). Infiltration in LX112 resin (Ladd Research Industries ...
• Therapy Development for Microvillus Inclusion Disease using Patient-derived Enteroids

  Meri Kalashyan, et al., bioRxiv - Cell Biology 2023

  Quote: ... and the sections were placed on formvar (Electron Microscopy Sciences) and carbon-coated grids ...
• Therapy Development for Microvillus Inclusion Disease using Patient-derived Enteroids

  Meri Kalashyan, et al., bioRxiv - Cell Biology 2023

  Quote: ... and then post-fixed for 1 hr at 4°C in 1% osmium tetroxide (Electron Microscopy Sciences) in 0.1 M Sodium Cacodylate ...
• Therapy Development for Microvillus Inclusion Disease using Patient-derived Enteroids

  Meri Kalashyan, et al., bioRxiv - Cell Biology 2023

  Quote: ... was followed by embedding in flat bottom Beem capsules (Electron Microscopy Sciences). The resulting blocks were sectioned using a Leica Ultracut E ultramicrotome (Leica Microsystems ...
• Therapy Development for Microvillus Inclusion Disease using Patient-derived Enteroids

  Meri Kalashyan, et al., bioRxiv - Cell Biology 2023

  Quote: ... Samples were then immersion fixed in 2.5% glutaraldehyde (Electron Microscopy Sciences, Hatfield, PA), 2% formaldehyde (Electron Microscopy Sciences ...
• The Autophagy Receptor NBR1 Directs the Clearance of Photodamaged Chloroplasts

  Han Nim Lee, et al., bioRxiv - Cell Biology 2023

  Quote: ... and incubated with anti-rabbit secondary antibody conjugated to gold particles (Electron Microscopy Sciences) in the blocking solution for 1 h ...
• The Autophagy Receptor NBR1 Directs the Clearance of Photodamaged Chloroplasts

  Han Nim Lee, et al., bioRxiv - Cell Biology 2023

  Quote: ... the samples were infiltrated with Epon resin (Electron Microscopy Sciences) with increasing the concentration of Epon 10% (v/v) ...
• Activation of Ca²⁺ phosphatase Calcineurin regulates Parkin translocation to mitochondria and mitophagy

  Elena Marchesan, et al., bioRxiv - Cell Biology 2023

  Quote: ... They were then fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, Pennsylvania) for 10⍰min at room temperature ...
• Identification of glioblastoma stem cell-associated lncRNAs using single-cell RNA-sequencing datasets

  Rasmani Hazra, et al., bioRxiv - Cancer Biology 2023

  Quote: ... then fixed in freshly prepared 4% paraformaldehyde (PFA) (Electron Microscopy Sciences, 19200). Fixed cells were permeabilized with different concentrations (50% ...
• Femtomolar-level PCR-free quantification of microRNA cancer biomarkers in serum

  Anastassia Kanavarioti, bioRxiv - Biophysics 2023

  Quote: ... A 4% aqueous osmium tetroxide solution (0.1575 M OsO4 in ampules at 2 mL each) was purchased from Electron Microscopy Sciences, and 2,2’-Bipyridine 99+% (bipy ...
• The metabolic enzyme EXT1 is sufficient to induce the epithelial-mesenchymal transition program in cancers

  Balakrishnan Solaimuthu, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The next day cells were detached using detachment buffer (0.05% EDTA in PBS) for 5 minutes and harvested in 10% HI-FBS DMEM and fixed with 4% paraformaldehyde (PFA, no-15710, Electron microscopy sciences, USA) in PBS for 10 min at room temperature ...
• Profiling extracellular vesicles in circulation enables the early detection of ovarian cancer

  Ala Jo, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Filtered EVs were captured on a glass slide (Electron Microscopy Sciences). Following a 30-min incubation at RT ...
• Age-dependent structural reorganization of utricular ribbon synapses

  Susann Michanski, et al., bioRxiv - Cell Biology 2023

  Quote: ... utricular organs were treated with a 1.5% potassium ferrocyanide (EMS, USA) and 4% osmium tetroxide solution (v/v in 0.1 M sodium cacodylate buffer ...
• Age-dependent structural reorganization of utricular ribbon synapses

  Susann Michanski, et al., bioRxiv - Cell Biology 2023

  Quote: ... Pin 12.7 mm x 3.1 mm) with a silver filled epoxy (Epoxy Conductive Adhesive, EPO-TEK EE 129-4; EMS, USA) and polymerized at 60° overnight ...
• A hierarchical pathway for assembly of the distal appendages that organize primary cilia

  Tomoharu Kanie, et al., bioRxiv - Cell Biology 2023

  Quote: ... cells were grown on acid-washed 12 mm #1.5 round coverslips (72230-10, Electron Microscopy Sciences) and fixed either in 4% paraformaldehyde (433689M ...
• Gaudichaudione H inhibits Herpes Simplex Virus-1 replication by regulating cellular nuclear factor-κB in an interferon-γ-inde-pendent manner

  Jiling Feng, Yuexun Tang, Wenwei Fu, Hongxi Xu, bioRxiv - Cell Biology 2023

  Quote: ... and DAPI Fluoromount G (Electron Microscopy Sciences). Images were taken by a confocal microscope (Olympus).
• Calcium flux through ER-TGN contact sites facilitates cargo export

  Bulat R. Ramazanov, et al., bioRxiv - Cell Biology 2023

  Quote: ... and mounted on 12mm coverslips (Electron Microscopy Sciences) using ProLong Gold (Thermo Fisher Scientific) ...
• Calcium flux through ER-TGN contact sites facilitates cargo export

  Bulat R. Ramazanov, et al., bioRxiv - Cell Biology 2023

  Quote: ... on glass slides (Cat. #72290-04, EMS) and transfected with RUSH-COMP-EGFP and RUSH-LyzC-EGFP constructs using Lipofectamine 2000 according to standard protocol ...
• Calcium flux through ER-TGN contact sites facilitates cargo export

  Bulat R. Ramazanov, et al., bioRxiv - Cell Biology 2023

  Quote: ... fixed in 4% PFA (Electron Microscopy Sciences) in PBS for 10 min ...
• Drosophila oocyte specification is maintained by the dynamic duo of microtubule polymerase Mini spindles/XMAP215 and dynein

  Wen Lu, Margot Lakonishok, Vladimir I Gelfand, bioRxiv - Cell Biology 2023

  Quote: ... Ovaries were dissected in 1X PBS and fixed with 4% EM-grade formaldehyde (Electron Microscopy Sciences 16% Paraformaldehyde Aqueous Solution ...
• Drosophila oocyte specification is maintained by the dynamic duo of microtubule polymerase Mini spindles/XMAP215 and dynein

  Wen Lu, Margot Lakonishok, Vladimir I Gelfand, bioRxiv - Cell Biology 2023

  Quote: Ovaries were (1) dissected in 1X PBS and fixed in 4% EM-grade formaldehyde (Electron Microscopy Sciences 16% Paraformaldehyde Aqueous Solution ...
• Adhesion-based capture stabilizes nascent microvilli at epithelial cell junctions

  Caroline S. Cencer, et al., bioRxiv - Cell Biology 2023

  Quote: ... sections were passed through 3 separate blocks of optimal cutting temperature (OCT) compound (Electron Microscopy Sciences) to wash off the sucrose solution ...
• Adhesion-based capture stabilizes nascent microvilli at epithelial cell junctions

  Caroline S. Cencer, et al., bioRxiv - Cell Biology 2023

  Quote: ... One end of the tube was clamped with a hemostat and the tube was filled with room temperature 2% paraformaldehyde (PFA) (Electron Microscopy Sciences) with a syringe and metal cannula ...
• Identifying Fibrogenic Cells Following Salivary Gland Obstructive Injury

  Amber L. Altrieth, et al., bioRxiv - Cell Biology 2023

  Quote: ... Ten-micron (10 µm) sections were collected on Superfrost Plus glass slides (Electron Microscopy Sciences) using a Leica CM 1860 cryostat ...
• Identifying Fibrogenic Cells Following Salivary Gland Obstructive Injury

  Amber L. Altrieth, et al., bioRxiv - Cell Biology 2023

  Quote: ... and then mounted with Permount mounting medium (Electron Microscopy Sciences).
• Identifying Fibrogenic Cells Following Salivary Gland Obstructive Injury

  Amber L. Altrieth, et al., bioRxiv - Cell Biology 2023

  Quote: ... 50% tissue freezing media (Tissue Freezing Medium, Electron Microscopy Sciences) overnight at 4°C ...
• Identifying Fibrogenic Cells Following Salivary Gland Obstructive Injury

  Amber L. Altrieth, et al., bioRxiv - Cell Biology 2023

  Quote: ... and mounted with Permount mounting medium (Electron Microscopy Sciences).