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Citations for Gold Biotechnology :
1 - 30 of 30 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Biochemistry 2021Quote: ... the soluble fraction was loaded and purified through a Ni-NTA metal-affinity column (Goldbio). After washes with 20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The His6-tagged protein variants present in the supernatant were purified using Ni2+-NTA affinity chromatography (Goldbio) and dialyzed overnight at 4 ⁰C against 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... Ni-affinity resin (Gold Biotechnology or Invitrogen) chromatography was performed by gravity at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... soluble fractions were subjected to nickel affinity purification (GoldBio) after being exchanged into native binding buffer (20 mM Tris (pH 8.0) ...
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Lipid nanodiscs as a template for high-resolution cryo-EM structures of peripheral membrane proteinsbioRxiv - Biochemistry 2023Quote: Protease inhibitors and affinity resins were purchased from Gold Biotechnology, Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeled activators were incubated with Ni2+-NTA-agarose resin (Goldbio) at 4°C for 1 hr on a rotator ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was added to a wash buffer-equilibrated nickel-NTA affinity gravity flow column (GoldBio) and elution of the protein was achieved using 1 column volume of elution buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... Samples were purified using NiNTA resin (Goldbio, USA) and eluted with 250 mM imidazole in NiNTA A buffer ...
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bioRxiv - Immunology 2021Quote: ... and mAbs were purified with protein A agarose (GoldBio).
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bioRxiv - Immunology 2022Quote: ... and antibody was purified using protein A agarose chromatography (Goldbio). Sequences were obtained from PCR reaction products and annotated using the ImMunoGeneTics (IMGT)/V- QUEST database (http://www.imgt.org/IMGT_vquest/)51,52 ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was purified using glutathione agarose resin (Gold Biotechnology Inc.). The protein was subjected to TEV cleavage to remove the GST tag and then further purified using a Mono Q 5/50 GL anion exchange column (GE Healthcare Life Sciences) ...
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bioRxiv - Synthetic Biology 2023Quote: ... purified using Nickel NTA agarose beads (Catalog No. H-350-5, GoldBio), and eluted (500 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Nanobodies were purified from the periplasmic fraction by Ni-NTA chromatography (Gold Biotechnology) and dialyzed against PBS to remove imidazole ...
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bioRxiv - Microbiology 2020Quote: ... nanobodies were purified from the periplasmic fraction by Ni-NTA chromatography (Gold Biotechnology) and dialyzed against PBS to remove imidazole.
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bioRxiv - Immunology 2021Quote: ... RBD proteins were purified using protein A agarose resin (Goldbio CAT# P-400), followed by on-column cleavage with HRV3C protease (made in-house ...
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bioRxiv - Immunology 2020Quote: ... The RBD was purified using protein A agarose resin (GoldBio, Cat# P-400) and cleaving the Fc domain using HRV3C protease (made in house ...
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bioRxiv - Microbiology 2020Quote: ... Secreted hACE2-Fc and mACE2-Fc proteins were purified by protein A chromatography (Goldbio).
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Lipin 1 and lipin 1 ΔM-Lip proteins were purified using Ni-NTA resin (GoldBio). Eluted protein was applied to Streptactin-XT resin equilibrated with equilibration buffer (100 mM Tris ...
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bioRxiv - Immunology 2020Quote: Supernatant containing RBD-Fc was purified over protein A agarose resin (Goldbio, Cat# P-400), cleaved with HRV3C protease (made in house ...
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bioRxiv - Immunology 2021Quote: ... All GST fusion proteins were purified using Glutathione Agarose 4B (Gold Biotechnology, #G-250-50), and eluted with HEPES buffered saline (HBS ...
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bioRxiv - Microbiology 2023Quote: ... GST-KatG was purified from the supernatant by incubating lysate overnight with glutathione agarose resin (Goldbio), washed with 300mL 1X PBS ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology, Olivette, MO), eluted with 300mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: GST pulldowns were performed by loading 1 µg of purified GST or GST-p130 onto 15 µL of glutathione beads (GoldBio) in NETN (20 mM Tris pH8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... FN3-AFF sensors were purified from the soluble fractions of cell lysates using nickel nitriloacetate resin (Gold Biotechnology, St. Louis, MO) following the manufacturer’s protocols ...