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Citations for Gold Biotechnology :
1 - 50 of 79 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... Cell lysate was clarified by centrifugation and glutathione agarose resin (GoldBio) was added to affinity purify the GST-GGA3 ...
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bioRxiv - Microbiology 2020Quote: ... clarified cell lysates were purified by protein G affinity chromatography (Gold Biotechnology, Inc.). The b12 Fab ...
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bioRxiv - Biochemistry 2021Quote: Cell lysates of Arpin wild-type and mutants C204S and W195D were incubated with glutathioneagarose resin (GoldBio) for 2 h at 4°C (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... GST lysates were incubated with glutathione agarose resin (GoldBio; 50 μL resin per 0.5-1.5 mL of lysate) for 30 min at 4°C and then washed 6x (3x quick washes ...
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bioRxiv - Biophysics 2024Quote: ... Cleared lysate was loaded onto lysis buffer-equilibrated Ni-NTA resin (GoldBio, 5 mL resin per 50 mL lysate) and rocked gently for 1 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... GST lysates were incubated with glutathione agarose resin (GoldBio) for 30 min at 4°C and then washed 6x (3x quick washes ...
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bioRxiv - Biochemistry 2022Quote: ... FN3-AFF sensors were purified from the soluble fractions of cell lysates using nickel nitriloacetate resin (Gold Biotechnology, St. Louis, MO) following the manufacturer’s protocols ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was then loaded onto Co2+-charged TALON resin (GoldBio), equilibrated previously with the wash buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysates were incubated with equilibrated Glutathione agarose (Gold Biotechnology) for 2 h at 4 °C and the beads washed in a gravity column using 50 ml lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... the cleared cell lysate was loaded onto a gravity column with 1 mL bed volume of Glutathione agarose resin (Gold Biotechnology, #G-250-5). The resin was extensively washed ...
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bioRxiv - Biochemistry 2020Quote: ... Cleared lysate was added to 200 μL of Nickel beads (Gold Biotech) in a small drip column (BioRad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cleared lysates were incubated with 4 mL glutathione agarose resin slurry (GoldBio) for 1 h at 4° C to capture GST-KIX ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysates were fractionated on a Ni2+-NTA agarose column (Gold Biotechnology). RPA was eluted using cell resuspension buffer containing 400 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was run over a Co2+ agarose bead (GoldBio, H-310-25) column at 1 mL/min ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed by incubating the sections with Proteinase K (20 μg/ml in Tris-EDTA buffer) (Goldbio, St.Louis, MO) for 20 minutes at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were applied to GST agarose column (Glutathione agarose resin, GoldBio Cat# G-250-5) and washed 3 times in wash buffer 1x TBS + 1mM DTT) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... GST-KatG was purified from the supernatant by incubating lysate overnight with glutathione agarose resin (Goldbio), washed with 300mL 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology, Olivette, MO), eluted with 300mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were clarified via centrifugation and applied to 4 mL of pre-equilibrated Ni+-NTA resin (GoldBio) in a gravity flow column ...
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bioRxiv - Biochemistry 2020Quote: ... lysate was cleared at 24,000 xg for one hours and the supernatant incubated with glutathione agarose resin (Goldbio) for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... 200 µl diluted cells (2 x104 cells) and 50 µl (8 mg/ml) D-luciferin (GoldBio, USA) were loaded into each well ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were resuspended with 1% low-melt agarose (GoldBio) in PBS at 40°C at ~30 million cells/mL (Fig ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were resuspended with 1% low-melt agarose (GoldBio) in PBS at 40°C at concentrations of ∼15 million cells per milliliter and then loaded into wells of pre-formed phantoms consisting of 1% molecular biology-grade agarose (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in media containing 0.1mM D-luciferin (Goldbio) and placed under photomultiplier tubes (PMTs ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were selected in 200µg/mL hygromycin B (Gold Biotechnology), colonies isolated with trypsin-EDTA-soaked filter papers and expanded ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were selected using 200 μg/ml hygromycin B (Gold Biotechnology), and single colonies were isolated using trypsin-soaked filter paper ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were induced with various IPTG concentrations (GoldBio, Cat. # 2481C) at 37°C for 3.5 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were selected in 200 μg ml−1 Hygromycin B (Gold Biotechnology) and colonies were isolated and expanded ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then resuspended in 20 μl 1% low-melt agarose (GoldBio) in PBS at 40°C and loaded into the wells of preformed 1% agarose (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... cells were induced with 50 μM Isopropyl β-d-1-thiogalactopyranoside (IPTG) (GoldBio). Expression was carried out at 8 °C and cells were collected at 20 hours after inoculation by centrifugation at 5000×g for 20 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 200 μM auxin (Andole-3-Acetic Acid, Gold Biotechnology) and dTAG7 were added ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were washed in PBS 0.5% BSA (0.5% BSA, Goldbio 9048-46-8) in a round bottom 96-well plate (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... Cells were grown in LB media supplemented with 0.2 mg/l of ampicillin (Goldbio) at 37 °C until OD600 of 1.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with D-luciferin (Gold Biotechnology St. Louis, MO; LUCNA-1g) at 37°C and 5% CO2 for one hour in 24 well plate (200 μL/well) ...
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bioRxiv - Cancer Biology 2022Quote: ... All stable cell lines were selected using 2μg/ml of puromycin (Goldbio, P-600). For OVA overexpression ...
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bioRxiv - Microbiology 2021Quote: ... the cell lines were treated with two-fold doubling dilutions of nourseothricin (Gold Biotechnology), S-D ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were induced with 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, GoldBio). Post induction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 8 ug/ml of blasticidin (Gold Biotechnology, B-800-100) for 48 h to select for cells that had integrated the Xlone-mScarlet-Myo10 plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). Sanger sequencing confirmed plasmids that contained the correct protease insert ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). A green-white screen46 was used to inoculate assembled colonies in selection liquid media ...
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bioRxiv - Biophysics 2022Quote: ... C43 (DE3) cells and overexpressed in 1L minimal media A supplemented with ampicillin (Gold Biotechnology) as previously described ...
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bioRxiv - Biochemistry 2022Quote: ... and the cell pellet was resuspended in 0.5mg ml-1 luciferin (GoldBio; cat. no. LUCNA) solution prepared in 1X HBSS buffer (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... Media was aspirated and cells were resuspended in luciferin sodium solution (0.5mg/ml) (Gold Biotech) prepared in 1X Hanks Balanced Salt Solution (HBSS/Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... Each CRISPR/Cas9 plasmid construct was independently transformed into DH5-α Chemically Competent Cells (GoldBio) with chloramphenicol (25 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... the total number of target cells was measured by adding D-luciferin potassium salt (Goldbio) to a final concentration of 0.5 mg/ml and reading the bioluminescence with the Infinite F Plex microplate reader (Tecan) ...
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bioRxiv - Biophysics 2023Quote: ... The bacteria cells were lysed in buffer containing 50 mM HEPES pH 7.4 (#H400, Goldbio), 1.5 M NaCl ...