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Citations for Gold Biotechnology :
1 - 50 of 53 citations for 6 Bromo benzo c isothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... X-gal (5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside, Goldbio) was dissolved in dimethylformamide at 50 mg/ml ...
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bioRxiv - Plant Biology 2022Quote: ... X-GlcA ((5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid, Goldbio) in DMF (Dimethyl formamide) ...
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bioRxiv - Plant Biology 2022Quote: X-gluc (5-bromo-4-chloro-3-indolyl-b-D-glucopyranosiduronic acid) (Gold Biotechnology, St ...
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bioRxiv - Microbiology 2024Quote: ... and 40 μg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal) (GoldBio).
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bioRxiv - Molecular Biology 2022Quote: ... and 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal) for blue white selection (Gold Biotechnology, 2016).
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bioRxiv - Synthetic Biology 2023Quote: ... and 120 μl of 20 mg/ml 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-gal, GoldBio) were overlaid on LB agar plates ...
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bioRxiv - Microbiology 2020Quote: ... Strains were patched onto medium containing 50 g/ml X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Gold Biotechnology), 1 mM IPTG ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 5-bromo-4-chloro-3-indolyl-β-D-galactoside (X- GAL) (X4281C10) for blue-white screening were purchased from Gold Biotechnology (Olivette, Missouri). Lysozyme (L6876-10G) ...
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bioRxiv - Biophysics 2023Quote: ... and 6 μg/mL of Blasticidin (Goldbio, B-800-25) for two weeks ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 μg/mL carbenicillin (GoldBio C-103), 25 μg/mL chloramphenicol (GoldBio C-105 ...
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bioRxiv - Biochemistry 2022Quote: Chloramphenicol (Goldbio, cat. no. C-105-5)
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/ml carbenicillin (GoldBio, C-103) and 25 µg/ml chloramphenicol (GoldBio ...
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bioRxiv - Biochemistry 2020Quote: ... and His SUMO tags were cleaved with Ulp1 protease overnight at 4°C in Buffer C (25mM Tris-HCl pH 8.5, 150mM KCl, 2mM TCEP (GoldBio), 50mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.05% CHAPS (GoldBio, Cat# C-080-100). We pooled and concentrated peak fractions to 5-10μM before we aliquoted and flash froze with liquid nitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... at 1 mg/mL and elastase at 6 U/mL (E-240, Goldbio) for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... after 10 μM coelenterazine 400a (#C-320, Gold Biotechnology) addition ...
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bioRxiv - Bioengineering 2023Quote: Carbenicillin disodium (Gold Biotechnology cat. no. C-103-5)
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bioRxiv - Biochemistry 2023Quote: ... Then 10 µM of coelenterazine 400a (GoldBio, #C-320) was added to the cells and BRET readings were recorded simultaneously at λemission 410 ± 40 nm (RLU ...
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bioRxiv - Neuroscience 2021Quote: ... The internal standard (IS) was: 6-Chloro-DL-tryptophan (TRP_Cl; Goldbio, St Louis, USA). All standards ...
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bioRxiv - Biochemistry 2022Quote: ... 10μl of a 20μM coelenterazine 400A (GoldBio; cat. no. C-320) solution diluted in Krebs/HEPES was added 5min before the end of the stimulation period ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein expression was induced for ∼6 h by 1 mM isopropyl β-a-D-1-thiogalactopyranoside (IPTG; GoldBio) in an 18 °C shaker ...
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bioRxiv - Biochemistry 2020Quote: ... washed twice with PBS and once with 50 mM HEPES pH 7.6, and resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Nonidet-P40, 0.2 mM TCEP (Gold Biotechnology), and Roche cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Neuroscience 2023Quote: ... then stimulated with the coelenterazine 400A substrate (5 mM) (Gold Biotechnology #C-320-1) and read for 30 min on Mithras2 Plate Reader (Berthold ...
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bioRxiv - Plant Biology 2020Quote: ... The center 8-10 cm of the second leaf from 7-9 plants were cut into thin strips perpendicular to the veins and immediately submerged in 10 mL protoplasting solution (0.6 M mannitol, 10 mM MES, 15 mg/mL cellulase R-10 [GoldBio], 3 mg/mL Macerozyme R-10 [GoldBio] ...
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bioRxiv - Plant Biology 2021Quote: ... The center 8 to 10 cm of the second leaf from 10 to 12 plants were cut into thin strips perpendicular to the veins and immediately submerged in 10 mL protoplasting solution (0.6 M mannitol, 10 mM MES, 15 mg/mL cellulase R-10 [GoldBio], 3 mg/mL Macerozyme R-10 [GoldBio] ...
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bioRxiv - Cell Biology 2022Quote: ... animals were grown on 6 cm NGM plates that were supplemented with 250 μl of 5 mg/ml of hygromycin B (GoldBio) in M9.
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bioRxiv - Microbiology 2024Quote: ... Transgenic parasites were selected based on plasmid resistance (blasticidin-S-deaminase) to 6 µM blasticidin-S (Gold Biotechnology B-800-100). Transgenic parasites modified with the aptamer/TetR-DOZI system or pTEOE episomes were cultured in 0.5-1 µM anhydrotetracycline (aTc ...
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bioRxiv - Physiology 2022Quote: ... N2 worms were egg-prepped onto solid NGM with 50 μg/ml carbenicillin (GoldBio, C-103-5) and HT115(DE3 ...
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bioRxiv - Biophysics 2024Quote: ... at 18 °C for 16 hours after induction with 0.2 mM isopropyl-β-D-thiogalactoside (Goldbio, I2481C100). The cells were resuspended and disrupted in lysis buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... Fecal samples were collected regularly over 2-6 weeks and plated onto SAB and SAB with nourseothricin (100 μg/mL, Gold Biotechnology, N-500-1) plates.
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bioRxiv - Biochemistry 2021Quote: ... Next the BRET readings were taken in well-mode by adding coelenterazine 400a (cat. no. C-320, GoldBio) to a final concentration of 10 µM and luminescence emission intensities were simultaneously recorded at 410 ± 40 nm and at 515 ± 15 nm ...
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bioRxiv - Biophysics 2023Quote: ... The temperature was then set to 30 °C and protein expression was induced by adding 0.2 % arabinose (Goldbio). Cells were grown for additional 16 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... plants were incubated with an aqueous solution of 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A) for 30 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... spread on selective Luria broth (LB) agar plates containing 50 μg/mL carbenicillin (GoldBio Cat #: C-103-25), and grown in a 37 °C incubator overnight ...
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bioRxiv - Biophysics 2020Quote: ... 37 °C) to an OD600 of 0.6-0.8 and induced with 0.75 mM isopropyl β-D-1-thiogalactopyranoside (Gold Biotechnology) for 12 h ...
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bioRxiv - Biophysics 2022Quote: ... Cultures were grown to Abs600 of 0.3 at 37 °C and expression of the encoded construct was induced with 1.0 μM IPTG (Gold Biotechnology). Expression was allowed to continue at 37 °C for 2 h ...
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bioRxiv - Biophysics 2019Quote: ... Cultures were grown to Abs600nm of 0.3 at 37 °C and expression of the encoded construct was induced with 1.0 μM IPTG (Gold Biotechnology). Expression was allowed to continue at 37 °C for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were transferred onto fresh media and treated with 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A).
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bioRxiv - Microbiology 2023Quote: ... New England Biolabs] were grown at 37 °C in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 25 µg mL-1 chloramphenicol (GoldBio) ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
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bioRxiv - Biochemistry 2021Quote: ... Transformed cells were grown at 37°C until OD 0.3 then grown at 18°C until induction at OD 0.6 with 1 mM IPTG (Gold Biotechnology, Olivette, MO). Cells were grown for 15 hours then centrifuged after which the pellet was resuspended in 20 mM Sodium Phosphate pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... expression cultures were grown at 37 °C to an optical density (OD600) of 3.0 before induction with 500 μM isopropyl-β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology) and incubation overnight at 16 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were grown at 37 °C to an OD600 of 0.8 and then induced using 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG; GoldBio, 2481C) for 8 h at 30 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and cultured axenically at 21°C in HL5 media (Froquet et al., 2009) supplemented with 300 µg/mL Streptomycin Sulfate (Gold Biotechnology) on 10 cm plates ...
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bioRxiv - Microbiology 2020Quote: ... prepared in PBS and then incubated overnight at 37°C (without carbon dioxide) with freshly prepared staining solution (1mg/ml of X-gal (GoldBio Technology, USA) in 40 mM citric acid/sodium phosphate ...
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bioRxiv - Synthetic Biology 2021Quote: ... and to this 0.85 mL of liquid (70 °C) top agar (2XYT media + 0.6% agar) supplemented with 2% Bluo-Gal (GoldBio, CAS #97753-82-7) was added and mixed by pipetting up and down once ...
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bioRxiv - Molecular Biology 2020Quote: ... and grown at 37 °C in LB Broth Miller (EMD) overnight with the respective antibiotics (100 μg/ml ampicillin (Gold Biotechnology, Inc.)) for pAS06 plasmid and 30 μg/ml chloramphenicol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: Individual 6-day-old seedlings expressing a CCA1::LUC reporter grown under LD cycles at 22°C were arrayed on 1/2x MS + 1% Sucrose plates and sprayed with 5mM luciferin (GoldBio, Olivette, MO) prepared in 0.01% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... the temperature was reduced to 20 °C and protein expression was induced with the addition of IPTG (GoldBio, St. Louis, MO, USA) to a final concentration of 1 mM ...