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Citations for Cambridge Isotope Labs :
1 - 50 of 185 citations for Human Serine threonine protein kinase PAK 1 PAK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: For serine incorporation assays using [13C315N1]-serine (Cambridge Isotope Labs) samples were eluted from a PepMap C18 easy spray column (Thermo ...
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bioRxiv - Biochemistry 2020Quote: ... 13C315N-serine (Cambridge Isotope Laboratories) was added to a final concentration of 100 mg/L ...
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bioRxiv - Biochemistry 2020Quote: ... 2H3-serine (Cambridge Isotope Laboratories) was added to a final concentration of 100 mg/L ...
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bioRxiv - Cancer Biology 2023Quote: ... [U-13C] serine (Cambridge Isotope Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... [2,3,3-2H]-serine were purchased from Cambridge Isotope Laboratory ...
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bioRxiv - Molecular Biology 2022Quote: ... [2-13C]serine (Cambridge Isotope Laboratories, CLM-2013-PK), [2-13C]glycine (Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... and [2,2,3-2H]-serine (Cambridge Isotope, #DLM-582-0.5), mice were fed continuously throughout the infusion period (8:00 p.m ...
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bioRxiv - Biochemistry 2023Quote: ... An internal standard of L-Serine-15N,d3 (1 nmole final, Cambridge Isotope Labs, DNLM-6863) was added to each sample ...
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bioRxiv - Neuroscience 2020Quote: Deuterated L-serine (#DLM-161-PK) was from Cambridge Isotope Labs (Tewksbury ...
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bioRxiv - Cell Biology 2023Quote: ... 400μM 2,3,3-D3-L-Serine (Cambridge Isotope Laboratories, DLM-582) and 400μM unlabeled (Glycine Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Stable isotope tracing experiments with [U-13C]-serine tracer (Cambridge Isotope Laboratories) were performed in MEM (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... D3-L-serine and D4-ethanolamine and 13C3-glycerol were from Cambridge Isotope Laboratories (Andover ...
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bioRxiv - Biochemistry 2021Quote: ... L-[U-13C]-serine and L-[U-13C]-glycine were purchased from Cambridge Isotope Laboratories (Tewksbury ...
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bioRxiv - Cancer Biology 2022Quote: ... containing 140 μM 13C315N1-serine (catalogue no. CNLM-474-H-PK; Cambridge Isotope Laboratories). K562 cells were cultured in Plasmax supplemented with 10% dialysed FBS (catalogue no ...
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bioRxiv - Biochemistry 2022Quote: ... 1 % P/S and isotope-labelled D3-15N-L-serine (1 mM unless indicated otherwise) and D4-L-alanine 2 mM (Cambridge Isotope Laboratories, MA, USA). Cells were grown further in the labelling media for 16 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... [3-13C] serine (CLM-1572) and [U-2H]betaine (DLM-407-PK) were purchased from Cambridge Isotope Laboratories and stored in stocks of 10 mg/mL at −20°C ...
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bioRxiv - Biochemistry 2019Quote: ... The following internal standards were used in metabolomics analyses: D3-Serine (DLM-582-0.1, Cambridge Isotope Laboratories), and the METABOLOMICS AMINO ACID MIX STANDARD (contains 13C3 ...
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bioRxiv - Biochemistry 2019Quote: ... and the METABOLOMICS AMINO ACID MIX STANDARD (contains 13C3, 15N-Serine, cat #MSK-A2-1.2, Cambridge Isotope Laboratories)
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bioRxiv - Immunology 2021Quote: ... cells were transferred to serine/glycine free media containing 13C-U-glucose (2g/L) (Cambridge Isotope Laboratories, CLM-1396-PK) for additional 2 hours ...
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bioRxiv - Immunology 2023Quote: ... activated T cells were washed with PBS and re-cultured in TCM (lacking glucose and serine) containing 10% dialyzed FBS and uniformly labeled [13C]-Glucose (Cambridge Isotope Laboratories). T cells (5 x 106 per well in 6 well plates ...
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bioRxiv - Cell Biology 2020Quote: ... Data was normalized to 100 μg per sample and polar metabolites were extracted in a total volume of 275 μl of 40:40:20 (acetonitrile:methanol:water) with inclusion of internal standard d3N15-serine (Cambridge Isotope Laboratories, Inc. #DNLM-6863). Extracted samples were centrifuged at 10,000 × g for 10 min and an aliquot of the supernatant was injected onto LC/MS where metabolites were separated by liquid chromatography as previously described (111) ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µl of 2× kinase buffer containing 80 µM O18 ATP (OLM-7858-PK, Cambridge Isotope laboratories, Inc) and 1 µg of bacterial purified human PIP4Kα-GST was added and the reaction was incubated at 30 °C for 1 hour ...
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bioRxiv - Biochemistry 2019Quote: ... For LC-MS/MS based experiments the kinase assay buffer contained 80 [γM 18O-ATP (OLM-7858-20, Cambridge Isotope Laboratory).
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Genetics 2021Quote: ... coli kit (MS-CRED-KIT) was purchased from Cambridge Isotope Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... protein was expressed in M9 minimal media with 15N-NH4Cl (Cambridge Isotope labs) as the sole nitrogen source instead of 2xYT media ...
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bioRxiv - Cell Biology 2019Quote: 3 μL of human plasma were spiked with 3 μL amino acid isotope labelled internal standards (Cambridge Isotope Laboratories, #MSK-A2-1.2) and extracted with 250 μL –20 °C methanol for 10 min and centrifuged at 4°C for 10 min at 15 000 g ...
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bioRxiv - Immunology 2021Quote: ... complete CTL:AIM-V labeling medium consisted of glucose-free CTL:AIM-V with 5% heat-inactivated human AB serum supplemented with 11 mM uniformly labeled 13C-glucose ([U-13C]glucose) (Cambridge Isotope Laboratories). Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies ...
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bioRxiv - Microbiology 2023Quote: ... We spiked 13C and 15N labeled algal AAs or protein (Cambridge Isotope Laboratories, Inc., MA) into late-log phase ...
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Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: ... the protein was prepared by growing the cells in the M9 media containing 15NH4Cl (Cambridge Isotope Laboratories) as a sole source of nitrogen.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... 250 µL of each human CVL sample was added to 750 µL of methanol containing 200 nM 13C18-OA (Cambridge Isotope Laboratories, Inc. #CLM-460-PK), incubated on ice for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was expressed in BL21/DE3 Escherichia coli cells using M9 minimal media supplemented with 15NH4Cl (Cambridge Isotope Laboratories) induced at an OD600 of 0.6 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Biophysics 2023Quote: ... 15N-labeled and 15N-13C labeled (as required) TRBP2-dsRBD2 protein was prepared using 15NH4Cl and 13C-glucose (Cambridge Isotope Laboratories) as a sole source of nitrogen and carbon ...
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bioRxiv - Biophysics 2024Quote: ... to obtain the 15N uniformly single labelled protein or in a media containing 15NH4Cl (0.1% w/v) and 13C-labelled glucose (0.4% w/v) (Cambridge isotope Laboratories, Inc.) for 15N and 13C uniformly double labelled protein ...
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bioRxiv - Biophysics 2023Quote: ... Uniformly-labeled 13C 15N Sup35NM samples were prepared by growing BL21(DES)-Rosetta Escherichia coli in the presence of M9 media with 2 g L-1 D-glucose 1H,13C6 and 1 g L-1 of 15N ammonium chloride (Cambridge Isotope Labs, Cambridge, MA). Purified ...
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bioRxiv - Biophysics 2019Quote: ... and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs) as the sole nitrogen and/or carbon source ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
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bioRxiv - Biochemistry 2022Quote: ... and sortase A-compatible proteins were either expressed in Terrific Broth (TB) media or M9 media supplemented with 13C-glucose and/or 15NH4Cl (Cambridge Isotope Labs, Inc.) followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... the cells were transferred to fresh LB medium for the overexpression of the unlabeled protein or to M9 minimal medium supplied with 15NH4Cl (Cambridge Isotope Laboratories Inc.) as the only nitrogen source for the labeled protein overexpression ...
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bioRxiv - Biochemistry 2021Quote: ... cells while expression was carried out in LB media for non-labeled protein and M9 minimal media containing 15NH4Cl (Cambridge Isotope Laboratories, Tewksbury, MA) for 15N ...
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bioRxiv - Physiology 2020Quote: ... constant infusion (0.0085 mg•kg-1•min-1) of [ring-13C6]-L-phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA) from 1000 to the end of the protocol ...
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bioRxiv - Biochemistry 2019Quote: ... with 1 g 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source ...
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bioRxiv - Bioengineering 2022Quote: ... 100 % 1-13C glucose (Cambridge Isotope Laboratories), or unlabelled glucose ...
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bioRxiv - Biochemistry 2022Quote: ... 1 g/L 15NH4Cl (Cambridge Isotope Laboratories) and 50 μg/mL kanamycin ...
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bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...