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Citations for Cambridge Isotope Labs :
201 - 250 of 347 citations for 6 P TOLYL IMIDAZO 2 1 B THIAZOLE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 300 μL of LC/MS grade methanol containing a mixture of 17 isotope-labeled amino acids (200 nM, Cambridge Isotope Laboratories, MSK-A2-1.2). To this solution ...
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bioRxiv - Cell Biology 2022Quote: ... Polar metabolites were extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Tissues were homogenized by Bead Ruptor 24 (Omni International ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed twice with ice-cold 0.9% NaCl and polar metabolites extracted in ice cold 80% LC/MS grade methanol containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Extracts were vigorously shaken by vortex for 10 minutes at 4°C and spun at 14,000 rpm at 4°C for 10 minutes to remove insoluble cell debris ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... Protein was precipitated from 10 µl of sample by addition of nine volumes of 74.9:24.9:0.2 v/v/v acetonitrile/methanol/formic acid containing internal standards (valine-d8, Sigma-Aldrich; St. Louis, MO; and phenylalanine-d8, Cambridge Isotope Laboratories; Andover, MA). Precipitated material was cleared by centrifugation and the supernatant was injected directly onto a 150 x 2 mm 3 µm Atlantis HILIC column (Waters ...
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bioRxiv - Genomics 2023Quote: ... followed by metabolite extraction in ice cold 80:20 LC/MS grade methanol: water buffer containing 15N and 13C fully-labeled amino acid standards (Cambridge Isotope Laboratories #MSK-A2-1.2). Samples were vortexed for 10 minutes at 4°C and then centrifuged at maximum speed (> 14,000g ...
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bioRxiv - Neuroscience 2020Quote: ... with a pH of 7.4 containing 6.15 mM NaN3 and 4.64 mM sodium 3-[trimethylsilyl] d4-propionate (Cambridge Isotope Laboratories) was prepared ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in RPMI containing 2 mM 13C5,15N2-glutamine (Cambridge Isotope Labs) as the sole glutamine source for 2 hours before metabolites were extracted and quantified as described above with the exception that no internal standards were added to the extraction buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.05 U Phosphodiesterase I (Sigma # P3243)] and [15N]5-2’-deoxyadenosine ([15N]-dA) (Cambridge Isotope Laboratories # NLM-3895-25) as the internal standard to account for variations in sample handling and mass spectrometer response59 ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Cell Biology 2024Quote: ... LysoIP and MockIP samples were resuspended in LC-MS grade 80% methanol (v/v) with isotopically labeled amino acids (Cambridge Isotope Laboratories, Inc. Catalog #MSK-A2-1.2). Metabolites were profiled by liquid chromatography/mass spectrometry (LC/MS ...
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bioRxiv - Systems Biology 2024Quote: ... 10 µL of spent media samples were mixed with 10 µL of known concentration of isotopically labeled internal standards of amino acids (Cambridge Isotope Laboratory, Andover, MA, MSK-A2-1.2). 600 µL of ice cold HPLC grade methanol was added ...
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bioRxiv - Systems Biology 2021Quote: ... 13C-[6C]-labeled 2-deoxy-D-glucose (Cambridge Isotope Laboratories, Inc., 12 Ci/mmol); [1-3H] 2-Deoxy-D-glucose (ViTrax Radiochemicals) ...
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bioRxiv - Biochemistry 2019Quote: ... 20 min) and resuspended in M9 minimal media comprising 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, NLM-467-25) with 10 g/L glucose or 10 g/L 13C glucose (Cambridge Isotope Laboratories ...
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bioRxiv - Immunology 2019Quote: Cells were cultured in glucose-free media with 2 mM 13C6-glucose (Cambridge Isotope Laboratories) or [13C5]-ribose-inosine (Omicron Biochemicals ...
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bioRxiv - Cell Biology 2023Quote: ... Mice were then injected with [U13C] succinate (10 mg/kg, 2 min) (Cambridge Isotope Laboratories) via intravenous tail vein ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled media refers to EMM with 2% D-Glucose (U-13C6) (Cambridge Isotope Laboratories, Inc.). Once cells came into contact with labelled media ...
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Biophysics 2023Quote: ... in dry acetonitrile (10 ml) was added 2-bromoethanol-1,1,2,2-d4 (240 μl, Cambridge Isotope Laboratories) under argon ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a 2 mM solution of isotopically labeled sodium bicarbonate (NaH13CO3; Cambridge Isotope Laboratories Inc., Tewksbury, MA) was added to a batch culture of R ...
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bioRxiv - Developmental Biology 2021Quote: ... Islets were then incubated on a rotator plate at 95 RPM for 90 minutes at 37°C and 5% CO2 before being transferred to Eppendorf tubes and the basal KRB exchanged for a 0.9 ml volume of KRB containing either 3 mM (low) or 17 mM (high) [U-13C6] glucose (Cambridge Isotope Laboratories, CLM 1396). Islets were then incubated for 1 hour at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... MMA quantification was performed using 3 uL of plasma that was spiked in with [U-13C]-MMA (Cambridge Isotope Laboratories, cat# CLM-9426-PK) as previously described (31 ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
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bioRxiv - Biophysics 2022Quote: ... and 2 g/L U-[2H] glucose (Sigma-Aldrich, St. Louis, MO/Cambridge Isotope Laboratories, Inc., Tewksbury, MA). Cells were grown up to an OD600 of 0.65 at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µl of 2× kinase buffer containing 80 µM O18 ATP (OLM-7858-PK, Cambridge Isotope laboratories, Inc) and 1 µg of bacterial purified human PIP4Kα-GST was added and the reaction was incubated at 30 °C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... and 2 ng of [13C6]-IAA was added as an internal standard (Cambridge Isotope Laboratories, Andover, MA, USA). The sample was extracted for 2h at 4°C under continuous shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 g/L KNO3 or 1 g/L K15NO3 (Cambridge Isotope Laboratories), pH 5.8 ...
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bioRxiv - Physiology 2024Quote: WT and Rnlsmut NIT-1 cells were seeded in the same experimental setup as untargeted analysis and maintained in DMEM supplemented with heavy labeled 5mM [13C6]-glucose for 24h (Cambridge Isotope Laboratories cat. 110187-42-3). Two 6-well plates of WT cells were treated with 5μM PG for the duration of the experiment ...
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bioRxiv - Microbiology 2019Quote: ... we replaced the glucose in our standard HIGG media with deuterated 2-D glucose (Cambridge Isotope Laboratories, DLM-1271). During the isomerization of glucose to fructose ...
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bioRxiv - Cancer Biology 2022Quote: ... and re-plated in glutamine free media that was supplemented with either 2 mM 15N-glutamine (Cambridge isotope laboratories) or 2 mM GlutaMAX (represents the 0 hours timepoint of 15N-glutamine tracing) ...
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bioRxiv - Cancer Biology 2022Quote: ... the dried pellet was dissolved in 550 µl D2O containing 0.01 mg/ml Sodium 2,2-Dimethyl-2-Silapentane-5-Sulfonate (DSS; Cambridge Isotope). The samples were then vortexed and centrifuged at 16000 rpm for 12 min ...
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bioRxiv - Plant Biology 2023Quote: ... The plant materials were extracted with the extraction buffer containing 2 μM labeled Met (13C, 15N, Cambridge Isotope Laboratories), 10 μM DL-dithiothreitol (DTT ...
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bioRxiv - Microbiology 2023Quote: ... glycine (2-13C, 99%; 15N, 98%+), L-phenylalanine (D8, 98%), or tyramine:HCl (1,1,2,2-D4, 98%) (Cambridge Isotope Laboratories, Inc.) was added to BHIS containing 0.1% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... [2-13C] glycerol (99%) and an equimolar mixture of [U-13C3] glycerol (99%) (Cambridge Isotope Laboratories, Inc., Andover, MA, USA) and naturally labelled glycerol ...
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bioRxiv - Biochemistry 2021Quote: ... containing 0.11 mM of the internal standard DSS (sodium 2,2-dimethyl-2-silapentane-5-sulfonate, D6, 98%; Cambridge Isotope Laboratories) at pH 7.0 and vortex mixed for <1 min prior to transfer into 1.7 mm SampleJet NMR tubes ...
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bioRxiv - Plant Biology 2022Quote: ... 2-13C-acetate and U-13C-acetate (13C isotopic purity of 99%, sodium salts from Cambridge Isotope Laboratories (Eurisotop, France); Figure 1) ...
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bioRxiv - Immunology 2023Quote: 2×106 BMDCs were treated with 1mM uniformly labeled 13C lactate (L-LA*) (CLM-1579-N-0.1MG, Cambridge Isotope Laboratories), in the presence or absence of LPS (100ng/mL) ...
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bioRxiv - Molecular Biology 2024Quote: EGM-2 media was replaced with DMEM media containing D-Glucose (U-13C6, CLM-1396-PK, Cambridge Isotope Laboratories Inc.) 3 hours prior to collection ...
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bioRxiv - Systems Biology 2022Quote: ... in a 100 mM sodium phosphate buffered solution with 0.11 mM sodium 2,2-dimethyl-2-silapentane-5-sulfonate (DSS-D6; 98%; Cambridge Isotope Laboratories, Inc.). Once dry ...
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bioRxiv - Biochemistry 2023Quote: ... The 6% site-specific 13C/15N isotopically labelled oligonucleotide were synthesized on K&A H8 synthesizer using the 2’-deoxyadenosine phosphoramidite (98% 15N) purchased from Cambridge Isotope Laboratories (USA) ...
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bioRxiv - Biophysics 2023Quote: ... except that yeast cells were grown in SD-CSM media made with 2% (w/v) protonated 13C-depleted glucose (99.9% 12C, Cambridge Isotope Labs) as the carbon source ...
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bioRxiv - Cancer Biology 2023Quote: ... U-[13C]-Q labelling media consisted of DMEM supplemented with 2 mM L-glutamine (U-13C5; CLM-1822; Cambridge Isotope Laboratories), 10 mM glucose ...
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bioRxiv - Physiology 2024Quote: Mice were fasted for 2 hours then injected intraperitoneally (i.p.) with 1g/kg uniformly labelled U-13C-glucose (CLM-1396, Cambridge Isotope Labs) in saline ...
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bioRxiv - Developmental Biology 2020Quote: Larvae collected at 0 hpe were separated into four 1.5 mL tubes each containing 10 individuals and incubated with 1.2 mL FSW + 1.2 μl 10 mM 13C-arginine (Lot No. PR-21659, Catalog No. CLM-2070-0, Cambridge Isotope Laboratories, Inc.). From each tube ...
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bioRxiv - Immunology 2021Quote: CD138+ PCs were sorted on day 4 of in vitro differentiation and grown for 18 hrs in glucose free 1640 RPMI (Thermo) with dialyzed FBS supplemented with 2 g/L [U-13C6] glucose (Cambridge Isotope Laboratories). Metabolites were extracted and measured using Ultra High Performance Liquid Chromatography Mass Spectrometry (UHPLC-MS) ...
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bioRxiv - Plant Biology 2020Quote: ... 200 μL of 65% isopropanol /35% 0.2 M pH 7.0 imidazole buffer (Chen et al. 1988) was then added with [13C6] IAA internal standard (Cambridge Isotope Laboratories Inc.), and samples were extracted on ice for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... and re-plated for 12 hours in glutamine free media that was supplemented with either 2 mM 15N-glutamine (Cambridge isotope laboratories) or 2 mM GlutaMAX (Gibco Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... 3×106 cells/well were seeded in a 6-well plate and cultured in 2 mL assay media supplemented with 10% dialyzed FBS (Wisent) and either 25 mM U-[13C]-glucose (Cambridge Isotope Laboratories) and 4 mM glutamine (Sigma ...
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bioRxiv - Physiology 2020Quote: ... constant infusion (0.0085 mg•kg-1•min-1) of [ring-13C6]-L-phenylalanine (Cambridge Isotope Laboratories, Tewksbury, MA) from 1000 to the end of the protocol ...
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bioRxiv - Biochemistry 2019Quote: ... with 1 g 15NH4Cl (Cambridge Isotope Laboratories) as the sole nitrogen source ...