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Citations for Eppendorf :
1 - 50 of 294 citations for rno mir 206 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... RT PCR was performed using the RealPlex2 (Eppendorf). Expression was normalized to ß-Actin (Mm02619580_g1 ...
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bioRxiv - Immunology 2023Quote: ... and quantitative-RT-PCR was performed (Eppendorf realplex2 Mastercycler).
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bioRxiv - Cell Biology 2021Quote: ... and a custom primer set mix (Fluidigm) that were amplified for 18 cycles on a thermocycler (Eppendorf). Excess primers were removed from the samples by Exonuclease I (NEB ...
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bioRxiv - Immunology 2020Quote: ... and pre-amplified with a custom 96-target DeltaGene (Fluidigm) primer set for 18 cycles on a thermocycler (Eppendorf). Any excess primers from the pre-amplification reaction were removed by Exonuclease-I (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... RT-PCR was performed on Mastercycler gradient thermocycler (Eppendorf, Hamburg, Germany) using KOD-Plus-Neo (Toyobo) ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR reaction mixture was set up with purified DNA in a thermocycler (Eppendorf, Germany) under the following conditions with a total of 35 cycles ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-PCR was performed on a Mastercycler Pro thermocycler (Eppendorf, Westbury, NY). PCR products were visualized on a 1% agarose gel.
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bioRxiv - Biochemistry 2020Quote: ... RT-qPCR was performed using Mastercycler ep Realplex PCR thermal cycler machine (Eppendorf). Target expression was normalized to rp49 ...
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bioRxiv - Cell Biology 2020Quote: ... made in triplicate and placed in real-time (RT) PCR plates (Eppendorf, 951022015) with clear film (Eppendorf ...
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bioRxiv - Molecular Biology 2021Quote: ... no template control from reverse-transcription and no template control from PCR Endpoint RT-PCR was run together with target samples in a Mastercycler gradient Thermocycler (Eppendorf) under following conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time RT-PCR was performed on a MasterCycler Ep-Realplex thermal cycler (Eppendorf) or an Applied Biosystems QuantStudio™ 7 Flex Real-Time PCR (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was performed with EzPCR™ HS Plus 5X PCR master mix (Elpis-Biotech) with a master cycler gradient (Eppendorf®) kit as described above using gene-specific forward primer enolase-F and reverse primer enolase-R ...
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bioRxiv - Cell Biology 2019Quote: Reverse transcriptase-polymerase chain reaction (RT-PCR) was carried out on an Eppendorf Mastercycler thermocycler (Eppendorf, Hamburg, Germany) in a single step using the Qiagen OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Bioengineering 2024Quote: ... using 50 ng of Geneblock as template and 0.5 μM of forward and reverse primer in a PCR Mastercycler (Eppendorf), with initial heating at 98°C for 30s ...
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bioRxiv - Immunology 2021Quote: ... Resulting cDNA was diluted 1:8 and subjected to endpoint RT-PCR using Taq DNA Polymerase (GeneDirex Inc., Taoyuan, Taiwan) in a Mastercycler Nexus (Eppendorf) or Bio-Rad T100 thermocycler ...
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bioRxiv - Microbiology 2020Quote: ... we used the universal bacterial primers 27F and 1492F with the following PCR conditions on a Mastercycler pro (Eppendorf, Hamburg, Germany); 95 °C for 5 min for DNA denaturation ...
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bioRxiv - Plant Biology 2019Quote: All primers were used for PCR amplification of genomic DNA using a touchdown program on the Mastercycler nexus GSX1 (Eppendorf, Germany): 95°C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... and then connected to a Femtojet microinjector set (Eppendorf). Injection was performed in a 15° angle ...
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bioRxiv - Synthetic Biology 2024Quote: ... and an electroporator set to 2500 V (Eporator, Eppendorf). The plasmids used in this study are described in Table 1 and primers for PCRs are listed in Table 2.
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bioRxiv - Developmental Biology 2022Quote: ... and a set of two CellTram’s (CellTram Vario, FemtoJet, Eppendorf). Each respective microinjector are connected to a holding micropipette for immobilizing the zebrafish embryo ...
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bioRxiv - Cell Biology 2019Quote: ... and primers for the indicated genes (see Table S3 for primer sequences) using a Mastercycler EP Realplex thermocycler (Eppendorf). mRNA levels were normalized to ACT1 and fold change values were calculated by comparing the normalized expression at the indicated time to the expression of the target before CaCl2 addition.
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bioRxiv - Immunology 2022Quote: ... and the reactions were set up by the epMotion M5073 robot (Eppendorf) and carried out in the CFX384 Touch™ real-time PCR detection system (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 15-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and stored at -80 °C until further analysis ...
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bioRxiv - Microbiology 2021Quote: ... pelleted by centrifugation (300 rcf, 3 min, RT in 50-mL conical tubes; then 21,000 rcf, 2 min, RT in Eppendorf tubes), and sonicated in chilled PBS (1 s on ...
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bioRxiv - Biochemistry 2022Quote: ... After 5 min incubation at room temperature (RT) the sample was centrifuged (8 min, RT, 180 x g, Eppendorf centrifuge 5810R) to separate proteoliposomes from the non-incorporated protein and detergent ...
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bioRxiv - Molecular Biology 2021Quote: ... and left overnight RT in a Thermomixer R (Eppendorf) at 1200 rpm ...
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bioRxiv - Genomics 2020Quote: ... Acceleration and deceleration were set to 2 across all centrifugations (Eppendorf centrifuge 5804, Eppendorf). Plasma was aliquoted per 1500 µL and stored at -80°C until further processing.
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bioRxiv - Genomics 2020Quote: ... Acceleration and deceleration were set to 2 across all centrifugations (Eppendorf centrifuge 5804, Eppendorf). Plasma was aliquoted per 1500 µL and stored at -80°C until further processing.
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bioRxiv - Synthetic Biology 2020Quote: ... We then set up a BioFlo120 system with a 5 L bioreactor (Eppendorf, B120110002) and added 3 L of SC-His medium supplemented with 15% glucose after autoclaving ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR reactions were set up in triplicates using a liquid handling station (ep-Motion5073, Eppendorf) in 384 well qPCR plates ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μM Al.488-labeled TDP-43-MBP-His6 was set up in low binding tubes (Eppendorf) in aggregation buffer and incubated with or without 100 μg/ml His6-TEV protease ...
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bioRxiv - Cell Biology 2023Quote: ... dried in a vacuum concentrator at RT (SpeedVac Concentrator Plus, Eppendorf, USA) and stored at -80 °C for further analysis.
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bioRxiv - Cell Biology 2023Quote: ... the SF was spun at 1,000xg for 10 minutes at RT (Eppendorf 5415R with rotor F45–24–11 ...
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bioRxiv - Developmental Biology 2019Quote: ... 30-40 young adult hermaphrodites were injected in the germline using an inverted micro-injection set up (Eppendorf). After injection ...
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bioRxiv - Cell Biology 2022Quote: ... at RT for 2X 5min at 2,000 rpm in a ThermoMixer C (Eppendorf). Liquid was removed ...
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bioRxiv - Microbiology 2019Quote: ... and PCR was conducted using a MasterCycler gradient PCR thermal cycler (Eppendorf, NY). Quantitative PCR was set up with each sample being run on the plate in triplicate for each of the conditions ...
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bioRxiv - Microbiology 2023Quote: PCR reactions were conducted in a PCR Mastercycler (Eppendorf North America, Hauppauge, NY). A 20 μl reaction containing 1.0 μl of cDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 mM Tris/HCl pH 7.4) overnight at RT in a Thermomixer R (Eppendorf) at 1200 rpm ...
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bioRxiv - Genetics 2021Quote: ... 50 mM Tris/HCl pH 7.4) overnight at RT in a Thermomixer R (Eppendorf) at 1200 rpm ...
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bioRxiv - Neuroscience 2021Quote: PCR reactions were carried out on a Mastercycler Gradient PCR machine (Eppendorf, Hamburg, Germany). Standard 20 µl PCR reactions were performed [9] ...
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bioRxiv - Microbiology 2019Quote: ... and PCR was conducted using a Master Cycler gradient PCR thermal cycler (Eppendorf, NY). Using GAPDH as the housekeeping gene ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μM purified TDP-43-MBP-His6 variants (WT, 5D, 12D, 12A) were set up in low binding tubes (Eppendorf) in 35 μl aggregation buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (∼200 ul) was transferred to a 70uM filter (Fisher # NC1444112) set in a new 1.5 mL Protein LoBind tube (Eppendorf # 022431081). The tube with the 70 uM filter was spun on a mini-centrifuge (Ohaus # FC5306 ...
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bioRxiv - Bioengineering 2021Quote: ... X50s PCR Mastercycler (Eppendorf, Germany). was used with initial denaturation step 10 min at 95°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5mL PCR clean (Eppendorf # 022431021) or 96-well plates were used to process the samples.
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bioRxiv - Bioengineering 2023Quote: ... LoBind twin.tec PCR plates (Eppendorf) for a total volume of 60 µL at 1x master mix and 1x target concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... and PCR protocol was carried out in a standard PCR device (Eppendorf AG 22331, Hamburg). For the qPCR runs ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The PCR program was performed using an Eppendorf Mastercycler® pro PCR system (Eppendorf, Germany). Subsequently ...
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bioRxiv - Bioengineering 2019Quote: ... centrifuging for 5 min at 400 rcf (RT, Eppendorf 5430; Rotor: F-35-6-30), re-suspension in fresh medium and transfer to cultivation flask.