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Citations for Eppendorf :
1 - 36 of 36 citations for Recombinant Mouse Fas His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Genetics 2022Quote: ... StageTips were washed twice with 0.1% FA and peptides were eluted with 40% ACN/0.1% FA and then dried in a Speed-Vac (Eppendorf) at 45°C for 45 min.
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bioRxiv - Neuroscience 2021Quote: ... with an fixed angled-rotor (FA-45-6-30, Eppendorf) and the virus pellet was resuspended in OPC-Sato medium for 1 hour at 4°C on a shaker ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 13,000 rpm (rotor #,FA-45-30-11, Eppendorf) for 45 minutes at 20°C to remove small aggregates and particulate matter.
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bioRxiv - Biochemistry 2023Quote: ... previously spun down (Eppendorf 5427R, FA-45-30-11, 18000g, 10min), was manually injected onto a Superdex 200 increase 5/150 GL column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... for 20 minutes (Eppendorf centrifuge 5424 R, Rotor FA-45-24-11). A portion of each lysate (60-100 µL ...
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bioRxiv - Biochemistry 2020Quote: ... Debris was removed by centrifugation (Eppendorf Centrifuge 5427 R, Rotor FA-45-12-17) 13k rpm ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was syringe filtered into an HPLC vial (Eppendorf FA-45-24-11) using a 0.22 μm PVDF filter ...
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bioRxiv - Microbiology 2021Quote: ... and centrifuged for 3 min at 13806 rpm (Centrifuge 5424, FA-45-24-11, Eppendorf) before use ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation (20000 g, 15 min, 4 °C, 5810R, rotor FA-45-30-11 Eppendorf, Hamburg, Germany) the supernatant was passed over a C18 cartridge (500 mg ...
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bioRxiv - Microbiology 2019Quote: ... for 20 min at RT and centrifuged on 45° fixed angle rotor FA-45-30-11 (Eppendorf) for 20 min ...
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bioRxiv - Genetics 2021Quote: ... 000 rpm for 10 min at 4°C (Eppendorf 5424R with rotor Eppendorf FA-45-24-11). The supernatant was mixed with Laemmli Buffer containing β-mercaptoethanol and heated at 95°C for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 7800 rpm for 10 min (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf). The supernatant was filtered through 0.22-μm-pore-size filters (Merck Millipore ...
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bioRxiv - Genetics 2021Quote: ... 000 rpm for 10 min at 4°C (Eppendorf 5424R with rotor Eppendorf FA-45-24-11). The supernatant was mixed with Laemmli Buffer containing β-mercaptoethanol and heated at 95°C for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... All centrifugation steps were performed at 13806 rpm for 5 min (Centrifuge 5424, FA-45-24-11, Eppendorf). Recombinant E ...
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bioRxiv - Microbiology 2022Quote: ... 10 mM MgSO4) centrifuged on the following day (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf, Hamburg, Germany) at 10,000 g for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... Digested peptides were extracted from gels using 50% ACN solution with 2.5% formic acid (FA) and concentrated in speedVac concentrator (Eppendorf). The aliquot (1/10 ...
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bioRxiv - Cell Biology 2019Quote: ... Digested peptides were extracted from gels using 50% ACN solution with 2.5% formic acid (FA) and concentrated in speedVac concentrator (Eppendorf). The aliquot (1/10 ...
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bioRxiv - Microbiology 2021Quote: ... Two ml of phage lysate with 109 PFU/ml were centrifuged at 14,000 rpm (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... we further purified the extracts by treatment with 1% sodium dodecyl sulfate in NaP-EDTA buffer and centrifugation at 13,000 rpm for 10 min (rotor FA-24×2, Eppendorf). This purification process was repeated two times ...
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bioRxiv - Microbiology 2021Quote: ... 0.5-1 mL culture sample was harvested by centrifugation for 3 min at 13806 rpm (Centrifuge 5424, FA-45-24-11, Eppendorf) and resuspended in 100-500 µL 10 mM NaOH depending on the size of the cell pellet ...
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bioRxiv - Microbiology 2020Quote: ... and elution with 40 μL of 50% acetonitrile (AcN)/0.2% FA and the eluents were dried using a Vacufuge vacuum concentrator (Eppendorf, US). The resulted peptides were cleaned up in a HyperSep filter plate with bed volume of 40 μL (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... PMNs were sedimented for 10 min with 1000 × g at 4°C on 45° fixed-angle rotor FA-45-30-11 (Eppendorf). The supernatant was filtered by gravity through sterile PVDF 5.0 µm Millex syringe filters (Merck-Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were spun at 10,000 g for 15 min (spin 2 in the same Eppendorf FA-45-48-11 rotor) and the resulting supernatant is the tissue lysate (TL ...
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bioRxiv - Microbiology 2023Quote: ... 2ml of cells were collected at different time points and was centrifuged at 2,348g (FA-45-24-11, Eppendorf rotor) for 5 minutes at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Bacterial pellets obtained from 1 mL of cell culture centrifuged at 13,000 rpm (Eppendorf 5424 Centrifuge with Fa-45-11 Rotor) were thoroughly washed three times with 1 mL fresh LB medium to remove residual antibiotics and were re-suspended in 100 µL liquid LB medium ...
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bioRxiv - Microbiology 2022Quote: A lysate sample (1 ml) with 108 PFU/ml was centrifuged at 20,000 g (centrifuge 5430R, rotor FA-45-24-11HS; Eppendorf, Hamburg Germany) for 2 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... were centrifuged at 16,873 gav for 3 min in the FA-45-18-11 fixed angle (45°) rotor of an Eppendorf model 5418 centrifuge (Eppendorf AG, Hamburg, Germany). The pellets were resuspended in 1 mL of 2.5% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged Z-B dimers (to 25 nM in 25 µL) in a 1.5-mL LoBind tube (Eppendorf). Then canonical nucleosomes (from native or recombinant source ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids encoding wild type Rac1 tagged with GFP (Rac1-wt-GFP) and Rac1-T17N tagged with GFP (Rac1-DN-GFP) were kindly provided by Stefan Linder (University Hospital Hamburg-Eppendorf, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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bioRxiv - Neuroscience 2023Quote: ... insoluble PFF were produced by incubation of 300 µM recombinant α-syn in a LoBind reaction tube (Eppendorf GmbH, GE) with one borosilicate glass bead (d = 3.0 mm ...
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bioRxiv - Biochemistry 2024Quote: ... the pre-weighed mouse tissues were transferred to Protein LoBind 1.5 mL microcentrifuge tubes (Eppendorf) using 500 µL cold sterile DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... 1-2 fecal pellets were collected from each mouse by voluntary defecation into a sterile microtube (Eppendorf, Germany) and fecal samples were immediately placed on dry ice ...
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bioRxiv - Immunology 2022Quote: ... both femurs were removed from each mouse and inserted into a snipped-end 0.6 mL tube (Eppendorf, Hamburg, Germany) inserted into a 1.5 mL Eppendorf tube containing 1 mL of cRPMI medium ...
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bioRxiv - Neuroscience 2019Quote: ... 0.3 microliters of 1E12-1E13 gc/mL were injected subretinally in neonatal mouse pups using pulled glass needles controlled using Femtojet injector (Eppendorf).