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Citations for Eppendorf :
1 - 50 of 291 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was performed with EzPCR™ HS Plus 5X PCR master mix (Elpis-Biotech) with a master cycler gradient (Eppendorf®) kit as described above using gene-specific forward primer enolase-F and reverse primer enolase-R ...
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bioRxiv - Microbiology 2021Quote: ... and SYBRGreen PCR Master Mix (Eurogenetec) and data analyzed with the Realplex software (Eppendorf) to determine the cycle threshold (Ct ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplification was performed using EzPCR™ HS Plus 5X PCR master mix (Elpis-Biotech, Daejeon, Korea) with a master cycler gradient (Eppendorf, Hamburg, Germany). PCR conditions were as follows ...
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bioRxiv - Microbiology 2021Quote: ... The qPCR experiment was performed in 96-well PCR plate using Eppendorf Mastercycler® RealPlex with SYBR Green PCR Master Mix (Eppendorf, USA) as per the universal SYBR Green qPCR protocol where fluorescent product is detected during the last step of each cycle ...
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bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time PCR was carried out using Maxima SYBR Green qPCR Master Mix (2X) (Molecular Biology) and Eppendorf Realplex System (Eppendorf) following the manufacturers’ protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
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bioRxiv - Microbiology 2019Quote: ... similar reaction mixture of PCR except for 1×SYBR Green Real-time PCR Master Mix (Toyobo, Tokyo, Japan) was used and performed at Mastercycler EP realplex2 (Eppendorf, Germany). To estimate the copy numbers of aCPSF1 or RNAP captured genes ...
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bioRxiv - Microbiology 2021Quote: 96 μl of the final OIL-PCR master mix was aliquoted into a 500 μl deep well plate (Eppendorf 00.0 501.101). Nycodenz purified stool cells were diluted in PBS to 104 cells/μl in an 8-well PCR strip for multichannel pipetting ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was run with 2 μl of cDNA and qPCRBIO SyGreen master mix (Cat.# PB20.11-01, PCR Biosystems) on an Eppendorf Mastercycler Ep Realplex (Eppendorf, Hauppauge, NY) using a program of 15 min at 95°C ...
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bioRxiv - Microbiology 2023Quote: ... This PCR master mix was split into triplicates (16.7 μL each reaction) using an epMotion 5075 liquid handling robot (Eppendorf, Hamburg, Germany). Thermocycler conditions were 2 min at 95 °C ...
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bioRxiv - Plant Biology 2023Quote: ... RT qPCR was performed using 2×Hieff qPCR SYBR Green Master Mix (Yeasen, Shanghai, China) and PCR reactions and fluorescence detection were performed in a Mastercycler ep realplex (Eppendorf, New York, NY). ACTIN 2 (ACT2 ...
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bioRxiv - Plant Biology 2023Quote: ... PCRs were performed using the Master Cycler realPlex2 (Eppendorf) in a 96-well reaction plate according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... and PCR was conducted using a Master Cycler gradient PCR thermal cycler (Eppendorf, NY). Using GAPDH as the housekeeping gene ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR analysis was performed on Eppendorf Realplex Master Cycler (Eppendorf, Germany) using KAPASYBRFAST [Mastermix (2× ...
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bioRxiv - Immunology 2021Quote: ... The reaction master mix was added using an X-stream repeating pipette (Eppendorf, Hauppauge, NY) to the microtiter plates ...
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bioRxiv - Immunology 2022Quote: ... The reaction master mix was added using an X-stream repeating pipette (Eppendorf, Hauppauge, NY) to the microtiter plates ...
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bioRxiv - Pathology 2020Quote: ... The reaction master mix were added using an X-stream repeating pipette (Eppendorf, Hauppauge, NY) to the microtiter plates which were covered with optical film (cat ...
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bioRxiv - Pathology 2023Quote: ... qRT-PCR was performed using an Eppendorf Realplex2 Master Cycler (Eppendorf AG 223341, Hamburg, Germany). Raw qRT-PCR data were analyzed using the delta delta-CT (2-ΔΔCt ...
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bioRxiv - Immunology 2021Quote: ... The reaction master mix was added to the microtiter plates using an X-stream repeating pipette (Eppendorf, Hauppauge, NY) which were then covered with optical film (Thermo Fisher) ...
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bioRxiv - Plant Biology 2019Quote: ... For quantitative real time PCR 1 µl of cDNA was used per reaction with 2x SYBR-Green Master Mix (Peqlab) on a Mastercycler ep realplex S (Eppendorf). UBC (AT5G25760 ...
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bioRxiv - Biophysics 2023Quote: ... 4 µl ATP from the dilution series prepared for the master mix plate (Eppendorf tubes with maximum ATP conc. = 200 µM) were added to row A and row I on reaction stop plate ...
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bioRxiv - Systems Biology 2021Quote: ... The six 96-well master plates were then re-arrayed into the final 384 well v-bottom PCR Plates (Eppendorf #951020702).
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR was done with HOT FIREPol EvaGreen qPCR Mix Plus (Solis Biodyne, Tartu, Estonia) on RealPlex4 thermocycler (Eppendorf, Hamburg, Germany). For miRNA detection ...
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bioRxiv - Cell Biology 2022Quote: The beads were resuspended with 40 µL extension mix and incubated at 37°C on a PCR cycler (Eppendorf, UX-93944-39) for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR data was produced by Eppendorf Realplex2 master cycler (Eppendorf AG 223341, Hamburg) and analysis done using delta delta-CT (2□-□ΔΔCT ...
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bioRxiv - Microbiology 2021Quote: ... Amplification was conducted using a master cycler (Eppendorf) programmed with 1 cycle at 95 °C for 1 min ...
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bioRxiv - Physiology 2022Quote: ... on the Master cycler RealPlex4 platform (Eppendorf, Germany). The primer pairs and PCR conditions used are listed in Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and Master Cycler Pro Device (EPPE6324000.516, Eppendorf, DE). For RT-qPCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sequencing reactions were performed in the Master Cycler pro 384 (Eppendorf) using the ABI BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... and PCR was conducted using a MasterCycler gradient PCR thermal cycler (Eppendorf, NY). Quantitative PCR was set up with each sample being run on the plate in triplicate for each of the conditions ...
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bioRxiv - Microbiology 2023Quote: PCR reactions were conducted in a PCR Mastercycler (Eppendorf North America, Hauppauge, NY). A 20 μl reaction containing 1.0 μl of cDNA ...
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bioRxiv - Immunology 2019Quote: ... and fluorescence was quantified using an Eppendorf Master Cycler (Eppendorf, Hauppauge, NY). Relative gene expression levels were calculated using the ΔΔCt method (Livak and Schmittgen 2001) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was performed in a thermocycler (Eppendorf Master cycler X50 Thermocycler) using the following program ...
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bioRxiv - Neuroscience 2021Quote: PCR reactions were carried out on a Mastercycler Gradient PCR machine (Eppendorf, Hamburg, Germany). Standard 20 µl PCR reactions were performed [9] ...
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bioRxiv - Bioengineering 2021Quote: ... X50s PCR Mastercycler (Eppendorf, Germany). was used with initial denaturation step 10 min at 95°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5mL PCR clean (Eppendorf # 022431021) or 96-well plates were used to process the samples.
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bioRxiv - Bioengineering 2023Quote: ... LoBind twin.tec PCR plates (Eppendorf) for a total volume of 60 µL at 1x master mix and 1x target concentration ...
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bioRxiv - Microbiology 2021Quote: ... Amplifications were performed in an Eppendorf Master Cycler Model 5345 (Eppendorf, Hamburg, Germany). Each 25 uL reaction contained 12.5 uL 2X reaction mix ...
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bioRxiv - Plant Biology 2021Quote: ... All reactions were performed using a Wagetech Projects Master Cycler (Eppendorf, Hamburg, Germany) with an initial denaturing cycle of 5min at 95 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... and PCR protocol was carried out in a standard PCR device (Eppendorf AG 22331, Hamburg). For the qPCR runs ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The PCR program was performed using an Eppendorf Mastercycler® pro PCR system (Eppendorf, Germany). Subsequently ...
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bioRxiv - Developmental Biology 2022Quote: ... The mix was injected with a FemtoJet® 4i microinjector (Eppendorf).
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bioRxiv - Plant Biology 2021Quote: ... Nextera PCR program cycle was used for PCR amplification using Eppendorf Thermal Mastercycler (Eppendorf AG, Hamburg). The holding temperature was 10°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification was performed on Mastercycler® nexus SX1 PCR thermal cycler (Eppendorf AG, Hamburg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... 1.5-mL PCR clean (Eppendorf # 022431021) or 96 well plates were used to process the samples.
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bioRxiv - Cancer Biology 2022Quote: ... in 0.2 mL PCR tubes (Eppendorf). Tissue sections were subjected to whole genome amplification using the Ampli1 WGA Kit (Silicon Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) was performed in a Mastercycler® ep realplex real-time PCR system (Eppendorf, Germany). cDNA was amplified using a SYBR Green kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... in a Mastercycler PCR system (Eppendorf, Belgium). Each reaction was performed in a 10 μL total reaction mixture using 1 μL of the DNA sample and 0.5 μM final primer concentration (EntF*-PCR primers ...
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bioRxiv - Cell Biology 2021Quote: ... in a Realplex 4 PCR machine (Eppendorf), and values were normalized to rpl-32 as an internal control ...