Labshake search
Citations for Eppendorf :
1 - 50 of 261 citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
-
bioRxiv - Developmental Biology 2023Quote: ... Tail biopsies of mice were lysed by a HotShot protocol (incubating the tail sample at 95°C for 30 min in an alkaline lysis reagent followed by neutralization) and used for PCR-based genotyping (GoTaq Green Master Mix, Promega, and Nexus X2, Eppendorf). Perinatal mice were also genotyped fluorescently (BLS miner’s lamp ...
-
bioRxiv - Cancer Biology 2024Quote: ... Slices used for single nuclei sequencing and genotyping were 20µm-thick and collected in Eppendorf® DNA LoBind tubes (Eppendorf, 0030108051). The number of slices per snRNA-seq or genotyping experiment ranged from 5 to 15 depending on the sample size ...
-
bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
-
bioRxiv - Microbiology 2019Quote: ... and PCR was conducted using a MasterCycler gradient PCR thermal cycler (Eppendorf, NY). Quantitative PCR was set up with each sample being run on the plate in triplicate for each of the conditions ...
-
bioRxiv - Microbiology 2023Quote: PCR reactions were conducted in a PCR Mastercycler (Eppendorf North America, Hauppauge, NY). A 20 μl reaction containing 1.0 μl of cDNA ...
-
bioRxiv - Neuroscience 2021Quote: PCR reactions were carried out on a Mastercycler Gradient PCR machine (Eppendorf, Hamburg, Germany). Standard 20 µl PCR reactions were performed [9] ...
-
bioRxiv - Microbiology 2019Quote: ... and PCR was conducted using a Master Cycler gradient PCR thermal cycler (Eppendorf, NY). Using GAPDH as the housekeeping gene ...
-
bioRxiv - Bioengineering 2021Quote: ... X50s PCR Mastercycler (Eppendorf, Germany). was used with initial denaturation step 10 min at 95°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1.5mL PCR clean (Eppendorf # 022431021) or 96-well plates were used to process the samples.
-
bioRxiv - Bioengineering 2023Quote: ... LoBind twin.tec PCR plates (Eppendorf) for a total volume of 60 µL at 1x master mix and 1x target concentration ...
-
bioRxiv - Molecular Biology 2022Quote: ... and PCR protocol was carried out in a standard PCR device (Eppendorf AG 22331, Hamburg). For the qPCR runs ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The PCR program was performed using an Eppendorf Mastercycler® pro PCR system (Eppendorf, Germany). Subsequently ...
-
bioRxiv - Plant Biology 2021Quote: ... Nextera PCR program cycle was used for PCR amplification using Eppendorf Thermal Mastercycler (Eppendorf AG, Hamburg). The holding temperature was 10°C ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification was performed on Mastercycler® nexus SX1 PCR thermal cycler (Eppendorf AG, Hamburg, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... 1.5-mL PCR clean (Eppendorf # 022431021) or 96 well plates were used to process the samples.
-
bioRxiv - Cancer Biology 2022Quote: ... in 0.2 mL PCR tubes (Eppendorf). Tissue sections were subjected to whole genome amplification using the Ampli1 WGA Kit (Silicon Biosystems ...
-
bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) was performed in a Mastercycler® ep realplex real-time PCR system (Eppendorf, Germany). cDNA was amplified using a SYBR Green kit (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2020Quote: ... in a Mastercycler PCR system (Eppendorf, Belgium). Each reaction was performed in a 10 μL total reaction mixture using 1 μL of the DNA sample and 0.5 μM final primer concentration (EntF*-PCR primers ...
-
bioRxiv - Cell Biology 2021Quote: ... in a Realplex 4 PCR machine (Eppendorf), and values were normalized to rpl-32 as an internal control ...
-
bioRxiv - Microbiology 2023Quote: ... in a 96-well PCR plate (Eppendorf) for a total volume of 0.1 ml ...
-
bioRxiv - Microbiology 2023Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed with an Eppendorf Mastercycler Ep Realplex instrument (Eppendorf AG, Hamburg, Germany) and the Kapa SYBR Fast qPCR kit (Kapa Biosystems ...
-
bioRxiv - Molecular Biology 2021Quote: ... no template control from reverse-transcription and no template control from PCR Endpoint RT-PCR was run together with target samples in a Mastercycler gradient Thermocycler (Eppendorf) under following conditions ...
-
bioRxiv - Molecular Biology 2019Quote: Digital PCR sample on the glass substrate was amplified in a flat-panel PCR machine (6335CH703993, Eppendorf AG 22331 Hamburg). 2-3 drops of mineral oil were placed onto the heating block to help with the heat transfer ...
-
bioRxiv - Biochemistry 2024Quote: ... 3.5 μL 0.1% TFA was added and the proteoCHIP LF 48 was cooled on wet ice to freeze the hexadecane again and allowed for manual separation from the sample containing aqueous phase by transferring the sample to individual wells of a low binding 96 well PCR plate (EP0030129512, twin.tec. PCR Plate 96 LoBind, skirted, Eppendorf, Darmstadt). The EVO96 proteoCHIP did not contain hexadecane and was directly placed on top of a 96well PCR plate to transfer the sample by centrifugation (500 g ...
-
bioRxiv - Microbiology 2019Quote: ... PCR was performed in a FlexID Mastercycler (Eppendorf) using Phusion High-Fidelity DNA Polymerase (New England Biolabs ...
-
bioRxiv - Microbiology 2021Quote: ... PCR was performed in a thermocycler (Eppendorf, Germany) under the following thermal cycling conditions ...
-
bioRxiv - Cancer Biology 2020Quote: ... 25μl was transferred to a PCR plate (Eppendorf) and stored at −80°C for subsequent genotyping ...
-
bioRxiv - Cell Biology 2020Quote: ... RT PCR was performed using the RealPlex2 (Eppendorf). Expression was normalized to ß-Actin (Mm02619580_g1 ...
-
bioRxiv - Neuroscience 2020Quote: ... A Mastercycler 5333 PCR thermal cycler (Eppendorf, Germany) was used to perform reverse transcription and pre-amplification ...
-
bioRxiv - Molecular Biology 2022Quote: ... The qRT-PCR data was produced by Eppendorf Realplex2 master cycler (Eppendorf AG 223341 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Quantitative PCR was performed on Mastercycler realplex4 (Eppendorf) using KOD SYBR® qPCR Mix (TOYOBO ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using Mastercycler® pro (Eppendorf) using the following cycling program ...
-
bioRxiv - Bioengineering 2023Quote: ... The PCR machine (Eppendorf AG 22331, Hamburg, Germany) was programmed as follows ...
-
bioRxiv - Microbiology 2021Quote: ... The qPCR experiment was performed in 96-well PCR plate using Eppendorf Mastercycler® RealPlex with SYBR Green PCR Master Mix (Eppendorf, USA) as per the universal SYBR Green qPCR protocol where fluorescent product is detected during the last step of each cycle ...
-
bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was performed with EzPCR™ HS Plus 5X PCR master mix (Elpis-Biotech) with a master cycler gradient (Eppendorf®) kit as described above using gene-specific forward primer enolase-F and reverse primer enolase-R ...
-
bioRxiv - Molecular Biology 2020Quote: ... The PCR was performed in a Thermocycler (Eppendorf mastercycler) with an initial denaturation step at 95°C for 3 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... in a Mastercycler® ep realplex PCR device (Eppendorf). The annealing temperature was 60 °C and the elongation time at 72 °C was 30 sec ...
-
bioRxiv - Microbiology 2020Quote: ... PCR program in a Mastercycler Personal-system (Eppendorf, USA) was 94°C for 5 min ...
-
bioRxiv - Microbiology 2020Quote: ... PCR amplification was performed using a Mastercycler gradient (Eppendorf) with 95 °C for 10 min ...
-
bioRxiv - Synthetic Biology 2022Quote: ... on a Mastercycler® nexus PCR Thermal Cycler (Eppendorf): 42°C for 60 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... and used for PCR in a thermocycler (BioRad, Eppendorf). Finally ...
-
bioRxiv - Immunology 2023Quote: ... and quantitative-RT-PCR was performed (Eppendorf realplex2 Mastercycler).
-
bioRxiv - Immunology 2022Quote: ... which were sealed with PCR microplate sealing foil (Eppendorf), and flash frozen prior to storage at −80°C.
-
bioRxiv - Microbiology 2023Quote: ... qRT-PCR was performed on a RotorGene Q (Eppendorf) using SensiFAST SYBR Hi-ROX (Bioline) ...
-
bioRxiv - Plant Biology 2023Quote: ... PCRs were performed using the Master Cycler realPlex2 (Eppendorf) in a 96-well reaction plate according to the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2021Quote: ... on a Mastercycler real-time PCR machine (Eppendorf, Hamburg, Germany). Results were normalized to three validated internal controls (actin ...
-
bioRxiv - Microbiology 2020Quote: ... the plate was covered with translucent PCR film (Eppendorf AG) and placed into the ImageXpress Micro XLS microscope into an environmental chamber with 37°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... The isolation was performed using 96-well PCR plates (Eppendorf) and a magnetic stand (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2020Quote: ... 25μl of cells were transferred to a PCR plate (Eppendorf) and stored at −80°C for subsequent genotyping using the same protocol as described for single cell in vitro assay.