Labshake search
Citations for Eppendorf :
1 - 50 of 1293 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... For that 2.5x105 cells were incubated with HIV-1 antigens in PBS/2% FCS for the indicated period of times on a 37°C thermomixer (Eppendorf). Cells were then fixed with IC fixation buffer on ice for 30 mins and at RT for additional 30 mins ...
-
bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
-
bioRxiv - Immunology 2021Quote: ... The culture supernatant containing the virus was centrifuged at 4,500 g for 5 min at 4°C (Eppendorf 5810R) and stored at -80°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Virus was removed from the upper chamber of the concentrator and stored in low protein binding tubes (Eppendorf #022431064) at -80°C.
-
bioRxiv - Microbiology 2020Quote: ... including human and microbial cells (4°C, Eppendorf 5810R centrifuge, 15 min, 3,220 rcf) represented in the “cell pellet” ...
-
bioRxiv - Biophysics 2021Quote: ... at a final ratio of 2.5 nmol DSS per 1 µg protein for 30 min at 37 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). After quenching by addition of ammonium bicarbonate to a final concentration of 50 mM and incubation for 10 min at 37 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
-
bioRxiv - Biophysics 2021Quote: ... and incubated for 1 hour at 37 °C (incubator Thermomixer C, Eppendorf, Germany). Control samples (no AMPs ...
-
bioRxiv - Biochemistry 2021Quote: ... Enzymatic digestion proceeded for 1 hr at 37 °C using the ThermoMixer C (Eppendorf) shaking at 400 rpm ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1mM EDTA) by heating to 95°C for 1 min in ThermoMixer C (Eppendorf) and cooling down to room temperature ...
-
bioRxiv - Synthetic Biology 2020Quote: ... at 25°C for 1 hour with shaking 1000 rpm (Thermo Mixer C, Eppendorf, Germany) in order to bind CD63+ EVs ...
-
bioRxiv - Microbiology 2023Quote: ... 1 min), annealing (65°C, 1 min), and elongation (72°C, 1 min) were performed in a Mastercycler thermal cycler (Eppendorf, Germany). A final extension step was added for 10 min at 72 °C at the end of the 30th amplification cycle ...
-
bioRxiv - Neuroscience 2021Quote: Supernatants from cells were collected and stored at −80°C in Protein LoBind Tube (Eppendorf). Cell lysates were scraped in RIPA buffer (Sigma-Aldrich ...
-
bioRxiv - Genomics 2022Quote: ... Beads and proteins were incubated for 3 hours at 4°C (Eppendorf ThermoMixer, 1,300 rpm). Beads were then washed four times with lysis buffer and recovered in 40 µl of laemmli buffer (50 mM Tris-Cl pH 6.8 ...
-
bioRxiv - Biochemistry 2023Quote: ... in buffer C for 1 h at 37°C and 500 rpm using a ThermoMixer (Eppendorf). The BS3 cross-linking reaction was stopped by incubation with 50 mM ammonium bicarbonate (NH4HCO3 ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were recovered in 1 mL YPD (1% yeast extract, 2% Bacto peptone, 2% D-glucose) at 30°C in a ThermoMixer C (Eppendorf, Hamburg, Germany) for 3 hours (200 RPM ...
-
bioRxiv - Neuroscience 2023Quote: ... Aliquots (500 µl) were stored at - 80°C in 1.5 ml tubes (Protein LoBind 0030108.116, Eppendorf) until use ...
-
bioRxiv - Cell Biology 2022Quote: ... and agitated (1,300rpm, 30 min, 1°C; Thermomixer Comfort, Eppendorf). Solubilized membrane proteins were obtained in the supernatant from this agitated suspension by centrifugation (21,000g ...
-
bioRxiv - Immunology 2019Quote: ... for 1 hour at 37°C in a Thermomixer (Eppendorf) with shaking set at 1000rpm ...
-
bioRxiv - Physiology 2023Quote: ... for 1 h at 4 □ on a ThermoMixer C (Eppendorf), 350 µl of water and 250 µl of chloroform were added to the mixture to induce phase separation ...
-
bioRxiv - Biochemistry 2022Quote: ... proteins were thawed and spun at 20,000 xg for 5 min at 20°C (Eppendorf microcentrifuge, Germany). The protein concentration was determined by measuring the absorbance at 11 = 280 nm and/or 11 = 488 nm (for GFP-labelled proteins ...
-
bioRxiv - Immunology 2023Quote: ... and aliquoted/stored in Dulbecco’s phosphate buffered saline (DPBS) at -80 °C in protein LoBind tubes (Eppendorf).
-
bioRxiv - Synthetic Biology 2020Quote: ... Samples were subsequently incubated at 25°C for 1 hour with shaking 1000 rpm (Thermo Mixer C, Eppendorf, Germany) in order to fluorescently label EVs ...
-
Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... Samples were incubated at 37°C for 5 days in 0.5 mL Protein Lobind tubes (Eppendorf, Hamburg, Germany) under continuous rotation at maximum speed (UVP HB-1000 Hybridizer ...
-
bioRxiv - Plant Biology 2022Quote: ... The captured-on beads proteins were digested overnight at 37°C under vigorous shaking (1200 rpm, Eppendorf Thermomixer) with 1 ug Trypsin/LysC (MS grade ...
-
bioRxiv - Developmental Biology 2021Quote: ... diluted 1:250 in 500ul PBSFBT either ON or for 2h at 32°C in a heating block (ThermoMixer C, Eppendorf) with integrated shaking (350rpm) ...
-
bioRxiv - Immunology 2019Quote: ... six cycles 94°C for 20 sec and a touchdown program of 15 cycles with successive annealing temperature decrement from 65°C to 60°C for 30 sec with 1°C decrement with a final extension at 72°C 10 min performed in a Mastercycler Gradient (Eppendorf). The products were monitored using 1.5 % agarose gel electrophoresis stained with ethidium bromide ...
-
bioRxiv - Developmental Biology 2023Quote: ... the DNA was eluted from the beads by incubating with 150μl elution buffer (100mM NaHCO3 and 1% SDS) for 20 mins at 65°C with vortexing using Eppendorf ThermoMixer C (Eppendorf). The supernatant was collected ...
-
bioRxiv - Biophysics 2021Quote: Bacterial suspensions were incubated with different concentrations of AMPs in buffer for 1 hour at 37 °C (incubator Thermomixer C, Eppendorf, Germany). Reference samples (no AMPs ...
-
bioRxiv - Neuroscience 2020Quote: ... conditioned media was collected and filtered through Spin-X 0.45 µM cellulose acetate centrifuge tube filter (#8163, Costar) and stored at −20°C in protein Lobind tubes (Eppendorf) until further usage ...
-
bioRxiv - Biochemistry 2019Quote: ... toluol and BCP for 1 min (21 °C, 2000 r.p.m, Eppendorf ThermoMixer) and centrifuged 20.000 ×g for 3 min ...
-
bioRxiv - Immunology 2022Quote: ... 20 μL of mAb solution was added to the cells in the filter wells and the cells were incubated for 1 h at 4 °C and 750 RPM on a thermomixer (Eppendorf, ThermoMixer C). After the incubation ...
-
bioRxiv - Systems Biology 2024Quote: ... are then added to the sample and incubated at 56°C for 1 hour at 1000 RPM (ThermoMixer C, Eppendorf, Hamburg, Germany). 37µl of 500mM chloroacetamide (50mM final concentration ...
-
bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
-
bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 3000 x g for 6 min at 4 °C and the conditioned media was transferred to a Protein LoBind tube (Eppendorf) (media fraction) ...
-
bioRxiv - Microbiology 2020Quote: ... printed arrays were moved to individual 1 mL centrifuge tubes containing 200 μL of M9 medium and melted at 55°C for 1 min in a thermomixer (Eppendorf ThermoMixer® C) shaking at 1000 rpm ...
-
bioRxiv - Biochemistry 2023Quote: ... The samples were then digested for 4 h at 37°C (1000 x rpm for 1 h, then 650 x rpm, Eppendorf ThermoMixer®C). Samples were then diluted 1:1 with milliQ water ...
-
bioRxiv - Biochemistry 2019Quote: ... pH 8.0) during 1 h at 37 °C and 1000 rpm (ThermoMixer, Eppendorf), as described in [12] ...
-
bioRxiv - Immunology 2019Quote: ... and (1×106) incubated at 37°C in centrifuge microtubes (Eppendorf, NY, USA) with or without a M ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1% bromophenol blue) and incubated at 40 °C in a Thermomixer R (Eppendorf) for 0.5 h ...
-
bioRxiv - Neuroscience 2023Quote: ... for 1 hour under shaking at 400 rpm using a ThermoMixer C (Eppendorf). Luminescent was read at the Cytation5M reader (BioTek) ...
-
bioRxiv - Cancer Biology 2024Quote: ... at a 1:50 ratio in a ThermoMixer C (Eppendorf, Nijmegen, The Netherlands) at 2,000 rpm for 18 hours at 37°C.
-
bioRxiv - Neuroscience 2020Quote: ... For bulk PFF production approximately 500μL of 5mg/ml monomeric protein was added to a 2-ml Eppendorf tube and placed on a thermomixer-C (Eppendorf, USA) shaker and incubated at 37°C and 1000RPM for up to 144 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... and incubated (gentle agitation, 20 minutes, 4°C) in EDTA solution (20 mM EDTA-PBS, Ca/Mg-free) in LoBind Protein tubes (Eppendorf 0030122216). The specimen was shaken ...
-
bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 1 minute on a thermal incubator (Eppendorf Thermomixer Option C, Germany). The tissue was then covered with 4% formaldehyde (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... in 25 mM AB) containing 50 µg of lysate protein and shaken overnight in a thermomixer (Eppendorf; 1400 rpm at 37 °C).
-
bioRxiv - Cell Biology 2022Quote: ... The crude extract was centrifuged for 15 min at 13,000 rpm at 4 °C to remove any tissue debris and the clear supernatant was transferred to a fresh Protein Low-Bind tube (Eppendorf, Hamburg, Germany). The total protein content of the samples was estimated using Pierce BCA protein assay kit from Thermo Scientific (Rockford ...
-
bioRxiv - Biochemistry 2022Quote: ... at 37°C at 1000 rpm in a Thermomixer-C (Eppendorf). Samples were mixed 1:1 (v:v ...
-
bioRxiv - Developmental Biology 2024Quote: ... for 5 min at 37 °C under agitation (Eppendorf, ThermoMixer C). Lobes were pipetted to promote dissociation ...
-
bioRxiv - Microbiology 2024Quote: ... and reactions incubated at 30°C in an ThermoMixer C (Eppendorf). Additional samples were collected at 15 ...