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Citations for Eppendorf :
351 - 396 of 396 citations for CTLA 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The 80 µL aliquot was lyophilised for 4 h at 30 °C with the aid of a vacuum concentrator (Eppendorf Concentrator 5301) attached to a refrigerated condensation trap (Savant) ...
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bioRxiv - Systems Biology 2021Quote: ... Total volume of 20 mL from each culture was transferred to a 50 mL Falcon® filled with ice and was immediately centrifuged at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). Next the supernatant was discarded and the cell pellet was snap frozen into liquid nitrogen and stored at -80 °C ...
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bioRxiv - Systems Biology 2021Quote: For the extraction of total proteome 10 mL of each culture were transferred into ice-cold 15 mL Falcon® tubes which were centrifuged immediately at 3000 rpm for 3 min at 4 °C (Eppendorf centrifuge). The supernatant from the centrifugation was discarded and the cell pellets were washed once with 1 mL of cold PBS buffer ...
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bioRxiv - Biophysics 2020Quote: ... 200 ml of M9 minimal media were added to 4 mL of pre-inoculum and the cell growth was monitored using Biophotometer D30 (Eppendorf, Hamburg, Germany) until the growth rate was 0.8 (OD at 600 nm) ...
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bioRxiv - Microbiology 2022Quote: ... coli S17-1 that contained the shuttle- or suicide-vector construct at 3700 rpm for 10 min at room temperature (Centrifuge 5920 R, rotor S-4×1000, Eppendorf, Hamburg, Germany). We mixed the E ...
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bioRxiv - Biophysics 2021Quote: ... Samples of 20 μΜ N-NTD or N-NTD-SR consisting of varied protein:nucleic acid molar ratios (8:1, 4:1, 2:1, 1:1, 1:2) were prepared in low-binding microtubes (Eppendorf® LoBind) in the presence of 10% PEG-4000 (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were pelleted by centrifugation at approximately 250 × g for 5 min at 4 °C (Eppendorf 5804 R, Hamburg, Germany). After resuspension ...
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bioRxiv - Cell Biology 2021Quote: Live-cell microinjection experiments were performed using a FemtoJet® 4i microinjector in conjunction with an InjectMan® 4 micromanipulation device (Eppendorf). All microinjections were performed using pre-pulled Femtotips® injection capillaries (Eppendorf) ...
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bioRxiv - Microbiology 2024Quote: ... The culture supernatant was collected by centrifugation (6,000 rpm, 10 min, 4°C; R10A2 rotor, Himac CR21F; Eppendorf Himac Technologies, Hitachinaka, Japan). For cultures grown under photoautotrophic conditions ...
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bioRxiv - Genetics 2024Quote: ... The samples were sonicated on ice for 12 to 15 minutes and then centrifuged for 10 minutes at 13200 rpm and 4 °C (Eppendorf centrifuge 5415R) to remove the cell debris ...
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bioRxiv - Microbiology 2023Quote: Liquid samples in 1 ml were collected and centrifuged for 20 min at 21 130 × g at 4°C (Centrifuge 5424 R; Eppendorf, Hamburg, Germany). The supernatants were used for chemical analyses by applying external standards for calibration and quality control ...
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bioRxiv - Zoology 2023Quote: ... The homogenates were centrifuged for 10 minutes (860 g at 4 °C) to remove the solid particulates (5810R, Eppendorf centrifuge, Hamburg, Germany) and the supernatant was removed and stored at -80 °C for later analysis [19] ...
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bioRxiv - Neuroscience 2023Quote: ... freshly laid embryos were aligned against nitrocellulose paper and injected using a quartz capillary needle, three-axis micromanipulator (Narishige, MMO-4) and microinjector (Eppendorf FemtoJet 4X).
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bioRxiv - Genomics 2023Quote: ... Cells were pelleted by spinning at 800 g for 10 min at 4 °C in a swinging bucket centrifuge (Eppendorf Centrifuge 5810R). The supernatant was gently removed ...
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bioRxiv - Biophysics 2023Quote: ... This pre-gel solution was kept at 4 °C inside an amber UV-protected 1.5 mL microcentrifuge tube (Eppendorf®, Hauppauge, NY), and degassed for 1 h inside a vacuum chamber to reduce oxygen concentration and prevent early gelation inside the driving syringe while running the experiment ...
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bioRxiv - Plant Biology 2023Quote: ... The primers were annealed at 95°C for 4 min followed by 70°C for 3 min in a thermocycler (Eppendorf, Hamburg, Germany). The reaction was allowed to cool slowly to room temperature ...
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bioRxiv - Microbiology 2023Quote: ... We performed fragmentation of 4 µg DNA in a 46-µL elution volume in Covaris G-tubes by centrifuging at 4200–5000 rpm (Eppendorf 5424 centrifuge) for 90 s to achieve fragment sizes of ∼20 kb ...
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bioRxiv - Systems Biology 2024Quote: ... with a mounted tissue section was dried to 37°C for 4 minutes on a thermal incubator (Eppendorf Thermomixer Option C, Germany) followed by in situ fixation in 4% PFA (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... To create extrachromosomal arrays constructs were microinjected into distal gonads of young adults using inverted microscope Leica DMi8 equipped with DIC filters and microinjection system InjectMan® 4 and FemtoJet® 4i (Eppendorf). Microinjection mixtures contained 10 ng/μL of the plasmid of interest ...
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bioRxiv - Microbiology 2024Quote: ... Pre-weighted metal-free 15 mL propylene tubes were used to pellet the cells in a tabletop centrifuge at 4 °C (Eppendorf, Hauppauge, NY). Pellets were washed three times with 10 mL of ice-cold PBS ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was centrifuged for 30 min at 15000 rpm at 4°C in the centrifuge Eppendorf 5702RH (Eppendorf SE, Hamburg, Germany). The pellet was resuspended in the 1/5 of the in initial volumes in PBS buffer (ThermoFischer Scientific ...
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bioRxiv - Developmental Biology 2024Quote: Chromatin was pre-cleared by centrifugation at 14,000×g for 15min at 4°C and the supernatant was moved to a 1.5mL DNA LoBind® tube (Eppendorf: Hamburg, Germany). From each chromatin sample per phenotype per lineage ...
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bioRxiv - Cell Biology 2024Quote: ... The sample was placed on ice to incubate for 30 minutes before the demembraned flagella supernatant was removed after centrifugation at 16,000 × g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D). The pellet containing the axoneme was resuspended in 245 μl of Cilia Final Buffer (without trehalose) ...
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bioRxiv - Plant Biology 2019Quote: ... and proteins were concentrated using Amicon ultracentrifugal 30-kDa cut-off filters for 30 min at 4000 x g at 4°C (Eppendorf 5810 R centrifuge). The purified recombinant protein was stored in storage buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... and proteins were concentrated using Amicon ultracentrifugal 30-kDa cut-off filters for 30 min at 4000 X g at 4°C (Eppendorf 5810 R centrifuge). The purified recombinant protein was stored in storage buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... The tubes were centrifuged at 18,000 x g and 4 °C for 15 min and the aqueous phase transferred to a 1.5 mL Eppendorf tube (Eppendorf UK Limited, Stevenage, UK). Two washes with 500 µl 24:1 chloroform/isoamyl alcohol were performed ...
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bioRxiv - Microbiology 2021Quote: ... The media was spun down in a centrifuge at 4°C for 10 min at 4400 g (Centrifuge 5920 R, Eppendorf Nordic, Hørsholm, Denmark) and the supernatant collected and stored at -80° for 1H NMR analysis ...
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bioRxiv - Microbiology 2021Quote: ... Development of ∼60 % cytopathic effect (CPE) led to harvesting and clarification of supernatants (3184 x g, 20 min, 4 °C, in an Eppendorf 5810 R centrifuge). Aliquots were then titred by plaque assay (see below) ...
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bioRxiv - Microbiology 2021Quote: The rest of the supernatant cell suspension (~19 ml) was centrifuged in a swing-out rotor (Eppendorf A-4-62 Swing Bucket Rotor) at 3200 × g for 10 min to pellet cells ...
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bioRxiv - Cancer Biology 2023Quote: ... at 4°C for 1 hour and centrifuged at 4°C at 14200 rpm for 50 min using a refrigerated table-top centrifuge (Eppendorf Centrifuge, Hamburg, Germany). The cleared lysates were loaded on wells of 96-well single-use filter micro-plates with 3 µm glass fibers and 25 µm polyethylene membranes (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... blood samples were allowed to clot at room temperature for 30 minutes before undergoing centrifugation at 1500 × g for 10 minutes at 4°C using a refrigerated centrifuge (Eppendorf AG, Hamburg, Germany). The supernatant serum was pipetted into sterile 1.5 mL Eppendorf tubes and stored at -80°C until further analysis.
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bioRxiv - Biochemistry 2024Quote: ... The samples were then digested for 4 h at 37°C (1000 x rpm for 1 h, then 650 x rpm, Eppendorf ThermoMixer®C). Samples were then diluted 1:1 with milliQ water ...
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bioRxiv - Physiology 2024Quote: ... Blood was allowed to clot on ice for 20 minutes then was spun down in a cold centrifuge (4°C, Eppendorf microcentrifuge, model 5415R) for 20 minutes at 2000 g ...
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bioRxiv - Cell Biology 2024Quote: The cilia suspension was thawed on ice and then centrifuged at 16000 g and 4°C for 10 minutes in a microfuge in a refrigerated room (Eppendorf, Centrifuge 5415 D). The pellet was resuspended in 250 μl of ice-cold Cilia Final Buffer (50 mM HEPES at pH 7.4 ...
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bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 4 minutes on a thermal incubator (Eppendorf Thermomixer Option C, Germany) and in situ fixed and washed as described above ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were spun down (5 min, 800 g•, 2 °C, minimal acceleration and break, Eppendorf 5810 R with swing-×bucket rotor A-4-44). The medium was discarded and the cells were dissociated in 15 mL of ice cold ACK solution (0.15 M NH4Cl ...
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bioRxiv - Cell Biology 2020Quote: ... This isolation method included a penultimate centrifugation step in Eppendorf polypropylene conical tubes (10,000 x g for 30 min at 4°C, in Eppendorf rotor F-34-6-38) that allowed the removal/isolation of larger microvesicles ...
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bioRxiv - Microbiology 2020Quote: ... The presumptive cultures were grown in TSBYE broth for 24 h at 30°C and centrifuged at 4°C (Eppendorf, 5810R, Auckland, New Zealand) at 3,220 × g for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... with incubation on ice for 1 h followed by centrifugation at 4 °C for 20 min at 20,000 g using an Eppendorf Centrifuge 5430R microcentrifuge (Eppendorf Canada Ltd, Mississauga, ON, Canada), supernatant combined and either used same day or stored at –80 °C.
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bioRxiv - Biochemistry 2023Quote: ... 12,000 rpm was applied for 30 minutes at 4 °C to obtain the supernatant and then evaporated to obtain a dry metabolite pellet (Eppendorf Concentrator plus, Hamburg, Germany). For the NMR experiments ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with 500μl of D-PBS and centrifuged at 400xg for 10 minutes at room temperature (Eppendorf Centrifuge 5810R, rotor A-4-62). The supernatant was removed and the cell pellet resuspended in 50μl of D-PBS before being stained with CD20-PE (clone A1SB12 ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated with ten-fold molar excess S-XL6 for 4 hours at 37 °C in protein low bind Eppendorf tubes using Eppendorf Thermomixer at 350 rpm (Eppendorf North America, Enfield, CT, USA). After incubation ...
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bioRxiv - Biophysics 2021Quote: ... Lipid extracts were resuspended in 60 μl 10mM ammonium acetate in methanol and diluted 1:4 in 96-well plates (Eppendorf twin.tec® 96; Sigma-Aldrich) prior to measurement of PC species ...
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bioRxiv - Biochemistry 2020Quote: Microdissected tissue samples were lysed in SDT buffer (4% SDS, 0.1M DTT, 0.1M Tris/HCl, pH 7.6) in a thermomixer (Eppendorf ThermoMixer® C, 30 min, 95°C, 750 rpm). After that ...
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bioRxiv - Microbiology 2022Quote: ... Trophozoites were harvested from their growth support by incubating the tubes by tapping the glass tubes followed by centrifugation (Eppendorf centrifuge 5810R, rotor A-4-62) according to a previously reported protocol 84.
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bioRxiv - Biochemistry 2021Quote: ... Unreacted dye was removed by two passages over an equilibrated Bio-spin® 6 column filled with Bio-gel P-6 media in labeling buffer and centrifuged in a tabletop centrifuge (Eppendorf 5810 R, rotor A-4-62) at 1500 rpm for 3 min ...