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Citations for Cellecta :
1 - 27 of 27 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: The inducible shRNA was obtained from Cellecta by cloning into a pRSIT16-U6Tet-sh-CMV-TetRep-2A-TagRFP-2A-Puro vector the following hairpin sequence:
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bioRxiv - Cancer Biology 2021Quote: ... Doxycycline inducible shRNA expression was accomplished by cloning these sequences into the pRSITEP-U6Tet-(shRNA)-EF1-TetRep-2A-Puro vector (Cellecta #SVSHU6TEP-L). shRNA seed matched negative control sequences 55 were generated by substituting complementary base pair sequences into positions 9-11 bp of the target shRNA using the web-based tool (https://web.archive.org/web/20180605134130/http://rnai.nih.gov/haystack/C911Calc2.html).
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bioRxiv - Cell Biology 2021Quote: ... or a scrambled shRNA (TAA TGT ATT GGA ACG CAT ATT) were cloned into the Dox-inducible shRNA expression vector pRSIT17 (Cellecta # SVSHU6T17-L), which was then transfected in combination with pVSV-G ...
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bioRxiv - Cancer Biology 2022Quote: The custom lentiviral shRNA library (Cellecta, Mountain View, CA, USA) consisted of 15,572 unique shRNAs ...
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bioRxiv - Cancer Biology 2021Quote: ... targeting sequences of shRNA were designed using a proprietary algorithm (Cellecta) and 10 shRNA targeting each gene were included in the library ...
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bioRxiv - Cancer Biology 2020Quote: The 27k Module 1 DECIPHER lentiviral shRNA library (Cellecta, DHPAC-M1-P) was transduced into C4-2B cells ...
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bioRxiv - Cancer Biology 2022Quote: Individual shRNAs were cloned into the pRSIT16-U6Tet-sh-CMV-TetRep-2A-TagRFP-2A-Puro vector (SVSHU6T16-L, Cellecta) using the following oligonucleotides:
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bioRxiv - Cancer Biology 2022Quote: ... the non-seed mature guide strand sequences have been previously demonstrated to be non-toxic.19 shRNAs were cloned in the pRSIT16-U6Tet-sh-CMV-TetRep-2A-TagRFP-2A-Puro vector (SVSHU6T16-L, Cellecta), that allows doxycycline induction ...
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bioRxiv - Molecular Biology 2019Quote: ... An MRE11-specific shRNA (5’ACAGGAGAAGAGAUCAACUUUGuuaauauucauagCAAAGUUGAUCUCUUCUCCUGU-3’) was expressed under doxycyclin control from pRSITEP-U6Tet-(sh)-EF1-TetRep-2A-Puro (Cellecta, Inc.). BacMam constructs were generated from pAceBac1 (Geneva Biotech ...
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bioRxiv - Cancer Biology 2022Quote: ... NOMO-1 and murine AML) were transduced with pooled barcoded lentiviral shRNA libraries targeting genes playing role in signaling pathway (Module 1, Cellecta Inc). The transduction efficiency was kept below 25% to minimize the number of cells with multiple integrations of the vectors ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-MB-231 cells were transduced with GFP labelled shRNA lentiviral particles (pRSIT17-U6Tet-sh-CMV-TetRep-2A-TagGFP2-2A-Puro, 1.30 × 107 TU, customized by Cellecta (Mountain View, CA) against OPA1 (5’-GTTATCAGTCTGAGCCA-3’ ...
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bioRxiv - Neuroscience 2020Quote: shRNA oligonucleotides were cloned into the DECIPHER pRSI12-U6-(sh)-HTS4-UbiC-TagRFP-2A-Puro lentiviral expression vector (Cellecta, cat. no. DVLIB-PS) following manufacturer’s instructions and then transformed into OneShot Top10 E.coli (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... with purified plasmid used as input for lentiviral packaging with second generation packaging plasmids psPAX2 and pMD2.G (Cellecta), transduced with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg of the plasmid of interest was combined with 10 μg of the packaging plasmid mix (Cellecta, psPAX2: pMD2.G) in DMEM without serum or antibiotics in the presence of Plus Reagent™ and Lipofectamine™ (Life Technologies) ...
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bioRxiv - Cancer Biology 2019Quote: ... with ClonTracer plasmid (10 μg per 10 cm dish) and lentiviral packaging and VSV-G plasmids psPAX2 and pMD2.G (Cellecta CPCP-K2A; 10 μg of mix per 10 cm dish). Supernatants of transfected HEK293T cells were harvested at 48 h and again at 72 h post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture chambers and transfected with the sgRNA library plasmid mix together with lentiviral packaging mix (Cellecta, containing psPAX2 and pMD2 plasmids that encode Gag/Pol and VSV-G ...
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bioRxiv - Cancer Biology 2023Quote: ... the above plasmids were transfected into HEK293T cells with the PC-Pack2 lentiviral packaging mix (Cellecta, Inc.), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... using 10 μg of library DNA and Ready-to-use Lentiviral Packaging Plasmid Mix (Cellecta, 0.5 μg/μL) per 10 cm dish (20 dishes in total) ...
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bioRxiv - Cancer Biology 2020Quote: ... In brief, concentrated lentiviral particles (TU, transducing units) from libraries or single plasmids were either purchased by Cellecta or produced by transfecting 293T cells according to the protocol in the Cellecta User Manual ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected with 1.84 µg of DNA of interest with 2.24 µg of ready-to-use lentiviral packaging plasmid mix (Cellecta) using Trans-IT transfection reagent (Mirus ...
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bioRxiv - Cancer Biology 2020Quote: CRISPR Cas9 (pRCCH-CMV-Cas9-2A-Hygro) and doxycycline inducible sgRNA (pRSGT16-U6Tet-(sg)-CMV-TetRep-2A-TagRFP-2A-Puro) plasmids were obtained from Cellecta. LentiX 293T cells and CalPhos transfection reagents (Clonetech ...
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bioRxiv - Cancer Biology 2021Quote: ... CRKL-V5 and EV lentivirus was produced by transfecting 293T packaging cells with pLenti6.3-CRKL-V5 or pLenti6.3-EV and Ready-to-Use Lentiviral Packaging Plasmid Mix (Cellecta #CPCP-K2A). WM3918 ...
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bioRxiv - Cell Biology 2021Quote: ... chip-based oligonucleotide synthesis was used to generate spacer-tracerRNA-encoding fragments which were PCR- amplified and cloned as a pool into the pRSI16 lentiviral plasmid (Cellecta). Lentiviral gRNA plasmids expressed a constitutive RFP and puromycin resistance to mark and select for infected cells respectively ...
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bioRxiv - Cancer Biology 2022Quote: Barcoded lentivirus libraries were synthesized using CloneTracker XP™ 10M Barcode-3’ Library with Venus-Puro (plasmid) (Cellecta, BCXP10M3VP-P) as described in Wang and McManus 2009 39 with polyethylenimine (PEI) ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK-293T cells were transfected with cellecta CloneTracker XP library and ready-to-use lentiviral packaging plasmid mix (Cellecta, CPCP-K2A) using Lipofectamine (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... SIDP library was packaged in lentiviral particles by large scale co-transfection of HEK293T cells with CELLECTA ready-to-use packaging plasmid (Cellecta – cat.no CPCP-K2A) using TRANSIT-LT1 transfection reagent (Mirus biologicals – cat ...
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bioRxiv - Molecular Biology 2023Quote: ... was used for MDA-MB-436 and mouse targeting shEXO1 (see Table 1 for sequence, cloned in pRSITEP-U6Tet-sh-EF1-TetRep-2A-Puro from Cellecta Catalog #: SVSHU6T16-L) was used for MEFs.