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1 - 24 of 24 citations for Two Hybrid System Construction kit since 2019

• Host metabolic reprogramming of Pseudomonas aeruginosa by phage-based quorum sensing modulation

  H. Hendrix, et al., bioRxiv - Microbiology 2019

  Quote: Bacterial two-hybrid assays were conducted using the BACTH System kit (bacterial adenylate cyclase two-hybrid system kit, Euromedex). The P ...
• A unique genomic island-governed cannibalism in Bacillus enhanced biofilm formation through a novel regulation mechanism

  Rong Huang, et al., bioRxiv - Microbiology 2021

  Quote: The Bacterial Adenylate Cyclase Two-Hybrid System (BACTH System Kit, Euromedex, France) was used to analyze protein–protein interactions ...
• Protein:Protein Interactions in the Cytoplasmic Membrane Influencing Sugar Transport and Phosphorylation Activities of the E. coli Phosphotransferase System

  Mohammad Aboulwafa, et al., bioRxiv - Microbiology 2019

  Quote: The Euromedex bacterial two-hybrid system (Euromedex, Cat # EUK001), consisting of two compatible plasmids pUT18 and pKNT25 ...
• UDP-glucose dehydrogenase Ugd in E. coli is activated by Gmd and RffD, is inhibited by CheY, and regulates swarming

  Irina A. Rodionova, et al., bioRxiv - Biochemistry 2020

  Quote: The Euromedex bacterial two-hybrid system (Euromedex, Cat # EUK001), consisting of two compatible plasmids pUT18 and pKNT25 ...
• PplT domain proteins – ubiquitous potential prokaryotic phospholipid translocases

  Janna N. Hauser, et al., bioRxiv - Microbiology 2022

  Quote: ... the commercially available bacterial two-hybrid kit (BATCH kit, Euromedex) was used [8 ...
• Transcriptional regulation of the N_ε-fructoselysine metabolism in Escherichia coli by global and substrate-specific cues

  Benedikt Graf von Armansperg, et al., bioRxiv - Microbiology 2020

  Quote: Protein-protein interactions were detected using the bacteria adenylate cyclase two-hybrid system kit (Euromedex) according to product manuals (Karimova et al. ...
• Molecular Basis of Cell Membrane Adaptation in Daptomycin-Resistant Enterococcus faecalis

  April H. Nguyen, et al., bioRxiv - Microbiology 2023

  Quote: Protein-protein interactions were evaluated using the Bacterial Two Hybrid System (Euromedex) according to the manufacturer instructions ...
• The Structures of SctK and SctD from Pseudomonas aeruginosa Reveal the Interface of the Type III Secretion System Basal Body and Sorting Platform

  Meenakumari Muthuramalingam, et al., bioRxiv - Biophysics 2020

  Quote: The PscK-PscDC interaction was tested using the bacterial adenylate-cyclase two-hybrid system (BACTH kit, Euromedex, France) [43 ...
• Vaccine antigen, Factor H binding protein, is typically a non-lipidated precursor that localises to the meningococcal surface by Slam

  RAG da Silva, et al., bioRxiv - Microbiology 2019

  Quote: The protein–protein interaction of MC58 FHbp and L91543 FHbp with SecA was investigated using the Bacterial Adenylate Cyclase Two-Hybrid System Kit (Euromedex) according to manufacturer’s instructions ...
• Xenogeneic regulation of the ClpCP protease of Bacillus subtilis by a phage-encoded adaptor-like protein

  Nancy Mulvenna, et al., bioRxiv - Microbiology 2019

  Quote: These were carried out using the Bacterial Adenylate Cyclase-based Two-Hybrid (BACTH) system (Euromedex) and were conducted as per manufacturer’s guidelines ...
• Mutation of mltG increases peptidoglycan fragment release, cell size, and antibiotic susceptibility in Neisseria gonorrhoeae

  Tiffany N. Harris-Jones, et al., bioRxiv - Microbiology 2023

  Quote: Bacterial Adenylate Cyclase Two Hybrid System experiments were performed according to the manufacturer’s instructions (Euromedex). Plasmids were created that expressed the protein of interest fused to T25 or T18 fragments ...
• Flagellar motor remodeling during swarming requires FliL

  Jonathan D. Partridge, et al., bioRxiv - Microbiology 2023

  Quote: Interactions between proteins of interest were screened using the BACTH bacterial two-hybrid system (Euromedex) as described by Karimova et al. ...
• Central role and structure of the membrane pseudokinase YukC in the antibacterial Bacillus subtilis Type VIIb Secretion System

  Matteo Tassinari, et al., bioRxiv - Microbiology 2020

  Quote: The Bacterial Two Hybrid (BACTH) assay was performed according to the manufacturer instructions (Euromedex). Plasmids used for the BACTH assays are listed in Table S2 ...
• The Group B Streptococcal surface antigen I/II protein, BspC, interacts with host vimentin to promote adherence to brain endothelium and inflammation during the pathogenesis of meningitis

  Liwen Deng, et al., bioRxiv - Microbiology 2019

  Quote: A bacterial adenylate cyclase two-hybrid assay was performed as in (32) and following manufacturer’s instructions (Euromedex). Briefly ...
• Listeria monocytogenes GlmR is an accessory uridyltransferase essential for cytosolic survival and virulence

  Daniel A. Pensinger, et al., bioRxiv - Microbiology 2021

  Quote: ... and pKNT25 from the BACTH System Kit (Euromedex) using XbaI and KpnI ...
• A Division of Labor in the Recruitment and Topological Organization of a Bacterial Morphogenic Complex

  Paul D. Caccamo, et al., bioRxiv - Microbiology 2019

  Quote: ... BACTH plasmids were made using the Euromedex BACTH System Kit (Euromedex Cat. No. EUK001).
• The DEAD-box RNA helicase Ded1 from yeast is associated with the signal recognition particle (SRP), and its enzymatic activity is regulated by SRP21

  Hilal Yeter-Alat, et al., bioRxiv - Biochemistry 2020

  Quote: ... Blots were washed two times at 42°C with 2X SSC buffer (Euromedex, Souffelweyersheim, France) with 0.1% SDS added ...
• Identification and characterization of novel filament-forming proteins in cyanobacteria

  Benjamin Lennart Springstein, et al., bioRxiv - Microbiology 2019

  Quote: ... the BACTH system (Euromedex) was employed ...
• Evidence for the Type IV Pili Retraction Motor PilT as a Component of the Surface Sensing System in Pseudomonas aeruginosa

  C.J. Geiger, G.A. O’Toole, bioRxiv - Microbiology 2023

  Quote: The B2H system from Euromedex (49) was used to assess protein-protein interactions in E ...
• Astrocyte-neuron interplay is critical for Alzheimer’s disease pathogenesis and is rescued by TRPA1 channel blockade

  Adrien Paumier, et al., bioRxiv - Neuroscience 2021

  Quote: ... chemiluminescence was detected using the ECL detection system (Euromedex) and a ChemiDoc MP Imaging System (Bio-Rad) ...
• Functional membrane microdomains and the hydroxamate siderophore transporter ATPase FhuC govern Isd-dependent heme acquisition in Staphylococcus aureus

  Lea Antje Adolf, et al., bioRxiv - Microbiology 2023

  Quote: ... the commercially available BACTH kit was used (Euromedex). In brief ...
• Visceral Fat Inflammation and Fat Embolism are associated with Lung’s Lipidic Hyaline Membranes in COVID-19 patients

  Georgia Colleluori, et al., bioRxiv - Pathology 2021

  Quote: Total RNA was extracted using the TRI-Reagent kit (Euromedex, Soufflweyersheim, France) and reverse transcription (RT ...
• MT5-MMP controls APP and β-CTF/C99 metabolism through proteolytic-dependent and -independent mechanisms relevant for Alzheimer’s disease

  Laura García-González, et al., bioRxiv - Neuroscience 2020

  Quote: ... The EasyBlocker kit was used to limit unspecific signal according to the manufacturer’s recommendations (GeneTex, Euromedex, Souffelweyersheim, France). In all cases ...
• Tissue-resident uterine regulatory T cells support fetal growth

  Laura Maria Florez, et al., bioRxiv - Immunology 2023

  Quote: ... composed of mouse endothelial cell medium with the addition of endothelial cell medium supplement kit (from Cell Biologics, Euromedex) containing fetal bovine serum ...