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Citations for Euromedex :
1 - 29 of 29 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Protein-protein interactions were evaluated using the Bacterial Two Hybrid System (Euromedex) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: Protein-protein interactions were detected using the bacteria adenylate cyclase two-hybrid system kit (Euromedex) according to product manuals (Karimova et al. ...
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bioRxiv - Microbiology 2023Quote: ... The strength of the interaction was determined by a β-galactosidase assay (per Euromedex instructions) after incubation at 30°C for 48-72 hours ...
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bioRxiv - Microbiology 2023Quote: Interactions between proteins of interest were screened using the BACTH bacterial two-hybrid system (Euromedex) as described by Karimova et al. ...
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bioRxiv - Microbiology 2019Quote: ... Interactions were quantified by beta-galactosidase assays from three colonies for each combination according to the protocol described by Euromedex or in a 96 well format according to Karimova ...
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bioRxiv - Biophysics 2020Quote: The PscK-PscDC interaction was tested using the bacterial adenylate-cyclase two-hybrid system (BACTH kit, Euromedex, France) [43 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were loaded on 10% SDS-PAGE gels in parallel with a protein prestained ladder (Euromedex) and transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: BACTH assays were performed according to the manufacturer’s protocol (Euromedex). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... stained with ClearSight DNA stain (Euromedex, France), and gel was scanned with Gel DocTM EZ Imager (Biorad) ...
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bioRxiv - Plant Biology 2023Quote: ... stained with ClearSight DNA stain (Euromedex, France), and the gel was scanned with Gel DocTM EZ Imager (Biorad) ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was induced using 40 μM IPTG (Euromedex) and carried out overnight at 18°C ...
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bioRxiv - Microbiology 2020Quote: The Bacterial Two Hybrid (BACTH) assay was performed according to the manufacturer instructions (Euromedex). Plasmids used for the BACTH assays are listed in Table S2 ...
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bioRxiv - Neuroscience 2024Quote: ... Molecular weight were checked using a Prestained Protein Ladder (#06P-0111, Euromedex).
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bioRxiv - Plant Biology 2021Quote: ... Proteins were transferred on nitrocellulose membrane 0.45 μm in 1xTris-Glycine (Euromedex®), 20%EtOH transfer buffer at 100 V for 1h ...
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bioRxiv - Microbiology 2022Quote: ... and plasmids verified by sequencing before BACTH assays (76) were set up according to the manufacturer (Euromedex). Co-transformation of plasmids containing fusion-genes of opposite domains ...
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bioRxiv - Microbiology 2019Quote: A bacterial adenylate cyclase two-hybrid assay was performed as in (32) and following manufacturer’s instructions (Euromedex). Briefly ...
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bioRxiv - Physiology 2020Quote: ... Genomic DNA was extracted using Proteinase K (20 mg/mL; Euromedex, France) and 1 mM of Tris-EDTA Buffer (pH = 8) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Genomic DNA was extracted using Proteinase K (20 mg/mL; Euromedex, France) and 1 mM of Tris-EDTA Buffer (pH = 8) ...
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: The qY2H assay was performed in pre-heated (30°C) and oxygenated SGR-UHW supplemented with Galactose 0.25% (Euromedex) and Raffinose 1% (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: Bacterial two-hybrid assays were conducted using the BACTH System kit (bacterial adenylate cyclase two-hybrid system kit, Euromedex). The P ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by migration at 135 V in 1X Tris-Glycine-SDS buffer (Euromedex). Proteins were electro-transferred on a nitrocellulose membrane in 1X Tris-Glycine buffer supplemented with 20% ethanol ...
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bioRxiv - Microbiology 2023Quote: ... Blue-fluorescent DNA stain 4’,6-diamidino-2-phenylindole (DAPI) was purchased from Euromedex (Souffelweyersheim, France). Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Blue-fluorescent DNA stain 4’,6-diamidino-2-phenylindole (DAPI) was purchased from Euromedex (Souffelweyersheim, France). Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Proteins were fractionated by performing SDS-PAGE (12% except where indicated) stained with Coomassie blue (Euromedex, Souffelweyrshim, France). For immunoblot analysis ...
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bioRxiv - Microbiology 2020Quote: Combinations of the above T18 and T25 fusion proteins were transformed into the BACTH compatible strain BTH101 (Euromedex) for analysis ...
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bioRxiv - Molecular Biology 2020Quote: ... for 1h in 37°C and proteins were then digested with Proteinase K (Euromedex, final concentration 0.4 mg/ml) for 2h at 37°C and the temperature was then shifted to 65°C overnight to reverse cross-links ...
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bioRxiv - Physiology 2023Quote: ... genomic DNA was isolated from mouse tail snip using lysis buffer Tris-HCl (EuroMedex, 26-128-3094-B) pH8.5 100mM ...
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bioRxiv - Plant Biology 2021Quote: ... Samples migrated on 7.5% (for proteins over 200kDa) or 10% SDS-PAGE polyacrylamide gel at 140V with 1xTris-Glycine SDS running buffer (Euromedex®). Proteins were transferred on nitrocellulose membrane 0.45 μm in 1xTris-Glycine (Euromedex®) ...
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bioRxiv - Microbiology 2022Quote: ... Equal amounts of GST or GST-Trim69 proteins were then bound to 10 μg of pure porcine brain tubulin (purchased from Euromedex, cat. CS-T240-A) in a total volume of 40 μl of PEM buffer supplemented with 40 μM of Taxol and 1mM GTP ...