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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for hsa mir 483 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... hsa-mir-423-5p (Thermofisher) served as endogenous control for analysis of miRNA expression ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-hsa-miR-Mir9-5p (Ambion, #17000), and Anti-miR negative control (Ambion ...
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bioRxiv - Neuroscience 2021Quote: ... The probes hsa-miR-499a-5p (Alexa Fluor 546; Thermo Fisher) and CamK2 (Alexa Fluor 488 ...
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bioRxiv - Immunology 2020Quote: ... Probes for TaqManTM MicroRNA Assay (hsa-miR-424-3p, hsa-miR-424-5p, U6 snRNA control) were purchased from ThermoFisher. All the steps were performed according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Taqman miRNA assays for the housekeeping control gene U6 snRNA (#001973) and hsa-miR-21-5p (which also recognizes mmu-miR-21a-5p) (#000397) were purchased from Thermo Fisher. qPCR was performed using Taqman Universal Master Mix II ...
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bioRxiv - Genomics 2023Quote: ... TaqMan™ MicroRNA Assay hsa-miR-423-5p (ThermoFisher, AssayID 002340, 4427975), and TaqMan™ Fast Advanced Master Mix (ThermoFisher ...
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bioRxiv - Physiology 2020Quote: mirVana hsa-miR-150-5p (ID MC10070) mimic or scrambled miRNA control (Ambion) in complex with DACC lipoplex preparation (Silence Therapeutics)(Fehring et al. ...
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bioRxiv - Neuroscience 2022Quote: ... we used hsa-miR-423-5p (suggested as endogenous control by ThermoFisher, Inc) for PD ...
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bioRxiv - Molecular Biology 2024Quote: ... 20nM hsa-miR-129-5p miRIDIAN mimics (miR-129 mimics) or mimic negative control (mimics ctrl)(ThermoFisher Scientific); 20nM has-miR-129-5p miRIDIAN hairpin inhibitor (miR-129 inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... or hsa-miR-181-5p) (Bioneer) or with a Pre-miR miRNA Negative Control (Bioneer) using Lipofectamine 3000 (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... real-time quantitative RT-PCR (Real-time Quantitative RT-PCR System, Thermo Fisher, USA) was used to quantify gene expression ...
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bioRxiv - Microbiology 2020Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). Primers were based on previous studies (Ding et al. ...
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bioRxiv - Pathology 2019Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). All gene specific primers used for qRT-PCR were evaluated by BLASTN search against the Fg and Bd genomes to exclude unspecific annealing and are listed in Supplemental Table 2 ...
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bioRxiv - Neuroscience 2024Quote: ... Primers used were: rno-miR-155-5p (RT:002571, ThermoFisher Scientific), and rno-miR-674-3p (RT:001956 ...
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bioRxiv - Cancer Biology 2020Quote: ... Capan-1 and Hs 766T were transfected with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218 (Ambion ...
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bioRxiv - Cancer Biology 2020Quote: ... Each cell line was transfected for 72 hours with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion ...
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bioRxiv - Cancer Biology 2020Quote: Capan-1 and Hs 766T were transfected for 72 hours with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time RT-PCR measurements were performed using the Step One Plus Real-Time Detection System (Thermo Scientific, Waltham, USA). Reactions were performed in triplicate in a total volume of 20 μl using the maxima SyBR green qPCR master mix according to manufacturer’s instructions (Thermo Scientific ...
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bioRxiv - Physiology 2021Quote: ... RT-qPCR was performed using the Real-Time PCR Detection System (Applied Biosystems StepOnePlus), 20 ng of total DNA and the SYBR® Green Master Mix (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... on a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system). Each sample was run in duplicate ...
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bioRxiv - Physiology 2023Quote: ... and a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system).29,30 Target gene expression was normalized to a housekeeping gene (ribosomal protein S18 ...
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bioRxiv - Biochemistry 2023Quote: ... with specific miR inhibitors (MH10203 for hsa-miR-22-3p and MH13382 for hsa-miR-1220-3p; Thermo Scientific) using Lipofectamine RNAiMax ...
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bioRxiv - Molecular Biology 2020Quote: ... and miR-183-5p (Applied Biosystems). Reverse transcription products were then amplified by quantitative PCR (95 °C ...
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bioRxiv - Cell Biology 2020Quote: Q-RT-PCRs were then performed using a 7900HT Fast Real-Time PCR detection system (Applied Biosystems) and standard qPCR program (1 cycle 95°C 15 min followed by 40 cycles 95°C 15s and 60°C 1 min) ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR was carried out using Sybr Green RT-PCR kits (Invitrogen) on a 7900HT fast real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time RT-PCR was conducted using the 7500 Real-Time PCR system (Applied Biosystems) with SYBR Green fluorescent probes using the following conditions ...
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bioRxiv - Pathology 2021Quote: Real-time RT-PCR was performed with the 7500 Real-Time PCR equipment (Applied Biosystems), using specific primers from PrimePCR SYBR Green Assay (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... transfected with the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p or Neg Control 1, Thermo Fisher) for 5-6 days ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons were co-transfected with 300ng of Cav1.2-HA at DIV6 and 10nM of the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p and Neg Control 1, Thermo Fisher), and processed for immunostaining 13 days after ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons were transfected at DIV 5 with 100ng of GFP alone or co-transfected with 10nM of the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p and Neg Control 1, Thermo Fisher). After 5 days of expression ...
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bioRxiv - Neuroscience 2021Quote: Hippocampal neurons were co-transfected at DIV 5 with 10nM of the miR-499-5p or control mimic (AmbionTM Pre-miR miRNA Precursor: miR-499-5p or Neg Control 1, Thermo Fisher) and 100ng of the Cacnb2 3’UTR luciferase reporters ...
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bioRxiv - Microbiology 2020Quote: ... Real-time RT-PCR assays were performed using AgPath-ID One Step RT-PCR kit (Life Technologies) and an ABI 7500 Real Time PCR system was used to perform the RT-PCR reactions 8 ...
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bioRxiv - Immunology 2021Quote: ... Real-time RT-PCR was performed in a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time RT-PCR measurements were performed using the StepOnePlus Real-Time PCR System (Applied Biosystems). Samples were analyzed in triplicate in a total volume of 20 µl using the maxima SYBR green qPCR master mix according to manufacturer’s instructions (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampal neurons were transfected at DIV7 with 10nM of the miR-499-5p or control mimic (Ambion™ Pre-miR miRNA Precursor: miR-499-5p and Neg Control 1, Thermo Fisher) with the Lipofectamine RNAiMAX reagent (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: mirVana™ miRNA Mimics and Inhibitors were used for hsa-miR-181a-5p overexpression/inhibition (Ambion™ - 4464066 and 4464084). HeLa cells were transfected with corresponding mirVana™ miRNA in 24 well plates at a density of 20.000 cells per well ...
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bioRxiv - Cancer Biology 2023Quote: ... both shβ7 cells and H295R cells were transfected with control miRNA mimics or hsa-miR-139-5p mimics (Thermofisher, #MC11749) at a final concentration of 10nM ...
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bioRxiv - Pathology 2020Quote: ... miR-155-5p or its control scrambled miR (Thermo Scientific Dharmacon) using Effectene (Qiagen ...
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bioRxiv - Pathology 2022Quote: ... RT-qPCR were performed on an ABI Q3 Real-time PCR Detection System (Applied Biosystems, USA) under the following conditions ...
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bioRxiv - Genomics 2023Quote: The mimic-miR-155-5p (Thermo Fisher, mirVanaTM miRNA Mimic ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using a QuantStudio 6 Flex Real-Time PCR detection system (Thermo Fisher) and PerfeCTa SYBR Green FastMix (Quantabio ...
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bioRxiv - Molecular Biology 2020Quote: Real Time quantitative PCR (RT-qPCR) was performed on a ViiA7 Real Time PCR System (Applied Biosystems). Reactions in a total volume of 10μl contained 2x SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Genetics 2020Quote: Real-time quantitative PCR (RT-qPCR) was performed in a StepOnePlus Real-time PCR system (Applied Biosystems) using 5x HOT FIREpol Eva Green qPCR Mix Plus (ROX ...
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bioRxiv - Cell Biology 2021Quote: ... Calu-3 cells were seeded at 2.5×105/ml with 100 or 300 nM of hsa-miR-145-5p miRNA precursor (PM11480, Ambion, Thermo Fisher Scientific). After 72 h ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative real-time PCR (RT-PCR) was performed by using StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, USA). All TaqMan gene expression assays (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-PCR analysis was performed using the ABI Prism HT 7900 real time PCR detection system (Applied Biosystems) equipped with SDS software version 2.4 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and the viral genomic copies were quantified using a real-time reverse transcription PCR (RT-PCR) (VetMax-Gold SIV Detection Kit, Life Technologies, Austin, TX). A standard curve was established using a chemically synthesized RNA fragment with known copy numbers ...
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bioRxiv - Genetics 2020Quote: ... hsa-miR-147b-3p and hsa-miR-210-3p levels were assessed by TaqMan Advanced miRNA Assays (Thermofisher) and TaqMan Fast Advanced miRNA master mix (Thermofisher) ...