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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for hsa mir 33a 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... with specific miR inhibitors (MH10203 for hsa-miR-22-3p and MH13382 for hsa-miR-1220-3p; Thermo Scientific) using Lipofectamine RNAiMax ...
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bioRxiv - Genetics 2020Quote: ... hsa-miR-147b-3p and hsa-miR-210-3p levels were assessed by TaqMan Advanced miRNA Assays (Thermofisher) and TaqMan Fast Advanced miRNA master mix (Thermofisher) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with hsa-miR-3p mimic (Invitrogen) or a control mimic at 5 nM final concentration using Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... or hsa-miR-106b-3p mirVana™ miRNA Mimic (Ambion) at final concentration of 5 nM ...
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bioRxiv - Pathology 2020Quote: ... Assay ID 245306_mat; 4427975, hsa-mir-23a-3p, Assay ID 000399; 4427975, cel-miR-39-3p, Assay ID 000200, Thermofisher). The relative expression of individual miRNA was calculated as ΔCt = mean Ct miRNA-mean Ct miR-39.
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using Oligofectamine (Invitrogen). Firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... Probes for TaqManTM MicroRNA Assay (hsa-miR-424-3p, hsa-miR-424-5p, U6 snRNA control) were purchased from ThermoFisher. All the steps were performed according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... hsa-miR-494-3p inhibitor (MIMAT002816) (Cat 4464084, Assay ID MH12409, Ambion). Negative Control LNA mimic (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... hsa-miR-494-3p mimic (MIMAT002816) (Cat 4404066, Assay ID MC12409, Ambion), mirVana miR inhibitor negative control #1 (Cat 4464076 ...
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bioRxiv - Genomics 2023Quote: ... TaqMan™ MicroRNA Assay hsa-miR-124-3p (ThermoFisher, AssayID 003188_mat, 4440886), TaqMan™ MicroRNA Control Assay U6 snRNA (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using (Lipofectamine RNAiMAX, Life Technologies). Twenty-four hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... and rno-miR-674-3p (RT:001956, ThermoFisher Scientific). Data were analyzed using StepOne Software v ...
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bioRxiv - Cell Biology 2021Quote: ... PLGA nanoparticles incorporating a mirVana miRNA mimic hsa-mir 132-3p (4464066, Thermo Fisher Science) were prepared using an emulsion solvent diffusion method in RNAase-free water ...
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bioRxiv - Neuroscience 2024Quote: ... we used rno-miR-191a-3p (RT:002576, ThermoFisher Scientific), which was selected as the most stable in our experiment ...
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bioRxiv - Cell Biology 2023Quote: ... real-time quantitative RT-PCR (Real-time Quantitative RT-PCR System, Thermo Fisher, USA) was used to quantify gene expression ...
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bioRxiv - Microbiology 2020Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). Primers were based on previous studies (Ding et al. ...
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bioRxiv - Pathology 2019Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). All gene specific primers used for qRT-PCR were evaluated by BLASTN search against the Fg and Bd genomes to exclude unspecific annealing and are listed in Supplemental Table 2 ...
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bioRxiv - Cancer Biology 2020Quote: Pre-miRNA precursor molecules for hsa-miR-210-3p and negative control 1 (Ambion/Thermo Fisher Scientific) were transfected into HK2 and HKC8 cells at a final concentration of 30 nM using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: Pre-miRNA precursor molecules for hsa-miR-210-3p and negative control 1 (Ambion/Thermo Fisher Scientific) were transfected into HK2 and HKC8 cells at a final concentration of 30 nM using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:200 cDNA was mixed with 1X hsa-miR-221-3p Advanced miRNA Assay (477981_mir, ThermoFisher-A25576) and ddPCR Supermix for Probes (Bio-Rad - 1863026 ...
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bioRxiv - Cell Biology 2021Quote: ... miR-30a-3p (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time RT-PCR measurements were performed using the Step One Plus Real-Time Detection System (Thermo Scientific, Waltham, USA). Reactions were performed in triplicate in a total volume of 20 μl using the maxima SyBR green qPCR master mix according to manufacturer’s instructions (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... hsa-mir-423-5p (Thermofisher) served as endogenous control for analysis of miRNA expression ...
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bioRxiv - Physiology 2021Quote: ... RT-qPCR was performed using the Real-Time PCR Detection System (Applied Biosystems StepOnePlus), 20 ng of total DNA and the SYBR® Green Master Mix (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... on a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system). Each sample was run in duplicate ...
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bioRxiv - Physiology 2023Quote: ... and a real-time PCR detection system (Applied Biosystems 7300 Real-Time PCR system).29,30 Target gene expression was normalized to a housekeeping gene (ribosomal protein S18 ...
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bioRxiv - Cell Biology 2020Quote: Q-RT-PCRs were then performed using a 7900HT Fast Real-Time PCR detection system (Applied Biosystems) and standard qPCR program (1 cycle 95°C 15 min followed by 40 cycles 95°C 15s and 60°C 1 min) ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR was carried out using Sybr Green RT-PCR kits (Invitrogen) on a 7900HT fast real-time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time RT-PCR was conducted using the 7500 Real-Time PCR system (Applied Biosystems) with SYBR Green fluorescent probes using the following conditions ...
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bioRxiv - Pathology 2021Quote: Real-time RT-PCR was performed with the 7500 Real-Time PCR equipment (Applied Biosystems), using specific primers from PrimePCR SYBR Green Assay (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Real-time RT-PCR assays were performed using AgPath-ID One Step RT-PCR kit (Life Technologies) and an ABI 7500 Real Time PCR system was used to perform the RT-PCR reactions 8 ...
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bioRxiv - Genomics 2019Quote: ... SH-SY5Y cells were transfected with either the hsa-miR-101-3p mimic (MC11414; Thermo Fisher Scientific, Waltham, MA, USA) or scrambled control (mirVana™ miRNA Mimic ...
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bioRxiv - Immunology 2021Quote: ... Real-time RT-PCR was performed in a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Real-time RT-PCR measurements were performed using the StepOnePlus Real-Time PCR System (Applied Biosystems). Samples were analyzed in triplicate in a total volume of 20 µl using the maxima SYBR green qPCR master mix according to manufacturer’s instructions (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... miR-320a-3p (Thermo Fisher Scientific, 478594_mir) was used as endogenous control ...
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bioRxiv - Pathology 2022Quote: ... RT-qPCR were performed on an ABI Q3 Real-time PCR Detection System (Applied Biosystems, USA) under the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using a QuantStudio 6 Flex Real-Time PCR detection system (Thermo Fisher) and PerfeCTa SYBR Green FastMix (Quantabio ...
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bioRxiv - Molecular Biology 2020Quote: Real Time quantitative PCR (RT-qPCR) was performed on a ViiA7 Real Time PCR System (Applied Biosystems). Reactions in a total volume of 10μl contained 2x SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Genetics 2020Quote: Real-time quantitative PCR (RT-qPCR) was performed in a StepOnePlus Real-time PCR system (Applied Biosystems) using 5x HOT FIREpol Eva Green qPCR Mix Plus (ROX ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative real-time PCR (RT-PCR) was performed by using StepOnePlus Real-Time PCR System (Thermo Fisher Scientific, USA). All TaqMan gene expression assays (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative RT-PCR analysis was performed using the ABI Prism HT 7900 real time PCR detection system (Applied Biosystems) equipped with SDS software version 2.4 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and the viral genomic copies were quantified using a real-time reverse transcription PCR (RT-PCR) (VetMax-Gold SIV Detection Kit, Life Technologies, Austin, TX). A standard curve was established using a chemically synthesized RNA fragment with known copy numbers ...
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bioRxiv - Microbiology 2021Quote: ... A 7500 Real time PCR detection system (Applied Biosystems) was used for RT-qPCR measurements using standard conditions ...
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bioRxiv - Neuroscience 2023Quote: ... and a Quantstudio5 Real-Time PCR Detection System (ThermoFisher). mRNA expression levels were normalized to the expression of the human housekeeping gene h18S and the cycle numbers plotted (hiPSC Trilineage Differentiation ...
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bioRxiv - Neuroscience 2023Quote: ... hsa-miR-221 (Thermo Fisher Scientific #000524) and hsa-miR-182 (Thermo Fisher Scientific #002334) ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-hsa-miR-Mir9-5p (Ambion, #17000), and Anti-miR negative control (Ambion ...
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bioRxiv - Physiology 2021Quote: ... TaqMan real-time RT-PCR assay (Thermo Fisher Scientific): mRNA expression of SMA (Hs00426835_g1) ...
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bioRxiv - Biochemistry 2021Quote: ... Quantitative real-time RT-PCR was performed on the ABI Prism 7900 Sequence Detection System (Applied Biosystems). Primer sequences were ...
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bioRxiv - Neuroscience 2020Quote: ... Real time RT-PCR was performed using the qPCR Master Mix kit (Thermofisher) and the WDR81 Forward/Reverse primers ...