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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... with specific miR inhibitors (MH10203 for hsa-miR-22-3p and MH13382 for hsa-miR-1220-3p; Thermo Scientific) using Lipofectamine RNAiMax ...
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bioRxiv - Bioengineering 2023Quote: ... and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... hsa-miR-147b-3p and hsa-miR-210-3p levels were assessed by TaqMan Advanced miRNA Assays (Thermofisher) and TaqMan Fast Advanced miRNA master mix (Thermofisher) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with hsa-miR-3p mimic (Invitrogen) or a control mimic at 5 nM final concentration using Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... or hsa-miR-106b-3p mirVana™ miRNA Mimic (Ambion) at final concentration of 5 nM ...
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bioRxiv - Pathology 2020Quote: ... Assay ID 245306_mat; 4427975, hsa-mir-23a-3p, Assay ID 000399; 4427975, cel-miR-39-3p, Assay ID 000200, Thermofisher). The relative expression of individual miRNA was calculated as ΔCt = mean Ct miRNA-mean Ct miR-39.
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using Oligofectamine (Invitrogen). Firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Immunology 2020Quote: ... Probes for TaqManTM MicroRNA Assay (hsa-miR-424-3p, hsa-miR-424-5p, U6 snRNA control) were purchased from ThermoFisher. All the steps were performed according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... hsa-miR-494-3p inhibitor (MIMAT002816) (Cat 4464084, Assay ID MH12409, Ambion). Negative Control LNA mimic (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... hsa-miR-494-3p mimic (MIMAT002816) (Cat 4404066, Assay ID MC12409, Ambion), mirVana miR inhibitor negative control #1 (Cat 4464076 ...
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bioRxiv - Genomics 2023Quote: ... TaqMan™ MicroRNA Assay hsa-miR-124-3p (ThermoFisher, AssayID 003188_mat, 4440886), TaqMan™ MicroRNA Control Assay U6 snRNA (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using (Lipofectamine RNAiMAX, Life Technologies). Twenty-four hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... and rno-miR-674-3p (RT:001956, ThermoFisher Scientific). Data were analyzed using StepOne Software v ...
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bioRxiv - Cell Biology 2021Quote: ... PLGA nanoparticles incorporating a mirVana miRNA mimic hsa-mir 132-3p (4464066, Thermo Fisher Science) were prepared using an emulsion solvent diffusion method in RNAase-free water ...
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bioRxiv - Neuroscience 2024Quote: ... we used rno-miR-191a-3p (RT:002576, ThermoFisher Scientific), which was selected as the most stable in our experiment ...
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bioRxiv - Cancer Biology 2020Quote: Pre-miRNA precursor molecules for hsa-miR-210-3p and negative control 1 (Ambion/Thermo Fisher Scientific) were transfected into HK2 and HKC8 cells at a final concentration of 30 nM using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: Pre-miRNA precursor molecules for hsa-miR-210-3p and negative control 1 (Ambion/Thermo Fisher Scientific) were transfected into HK2 and HKC8 cells at a final concentration of 30 nM using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:200 cDNA was mixed with 1X hsa-miR-221-3p Advanced miRNA Assay (477981_mir, ThermoFisher-A25576) and ddPCR Supermix for Probes (Bio-Rad - 1863026 ...
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bioRxiv - Cell Biology 2021Quote: ... miR-30a-3p (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... The multiplex RT primer pool consisted of primers for miR-150-5p and U6 (Thermo Fisher Scientific). No-template samples where included as negative controls.
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bioRxiv - Genetics 2020Quote: ... hsa-mir-423-5p (Thermofisher) served as endogenous control for analysis of miRNA expression ...
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bioRxiv - Physiology 2020Quote: mirVana hsa-miR-150-5p (ID MC10070) mimic or scrambled miRNA control (Ambion) in complex with DACC lipoplex preparation (Silence Therapeutics)(Fehring et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... as previously described (48) using the following primers: hsa-miR-21 (Thermofisher #0003970) and Z30 (Thermofisher #001092) ...
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bioRxiv - Genomics 2019Quote: ... SH-SY5Y cells were transfected with either the hsa-miR-101-3p mimic (MC11414; Thermo Fisher Scientific, Waltham, MA, USA) or scrambled control (mirVana™ miRNA Mimic ...
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bioRxiv - Cancer Biology 2020Quote: ... miR-320a-3p (Thermo Fisher Scientific, 478594_mir) was used as endogenous control ...
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bioRxiv - Cell Biology 2022Quote: ... for miR-375 was quantified by TaqMan Assays using the TaqMan MicroRNA Reverse Transcription Kit and hsa-miR-375 primer sets (Thermo Fisher Scientific, 000564). MiR-375 levels were normalized to miR-U6 expression ...
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bioRxiv - Neuroscience 2023Quote: ... hsa-miR-221 (Thermo Fisher Scientific #000524) and hsa-miR-182 (Thermo Fisher Scientific #002334) ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-hsa-miR-Mir9-5p (Ambion, #17000), and Anti-miR negative control (Ambion ...
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bioRxiv - Cancer Biology 2021Quote: ... Q-RT-PCR was performed using TaqMan primers/probe sets (Applied Biosystems) and analyzed as transcript copies per 50 ng RNA expressed relative to RNAPII expression as previously described (33).
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bioRxiv - Bioengineering 2022Quote: ... PACE60 nanoparticles were formulated as described previously by double emulsion solvent evaporation using miR200b mimic (Invitrogen mirVana miRNA Mimic, hsa-miR-200b-3p, 5’-UAAUACUGCCUGGUAAUGAUGAC −3’, Cat# 4464066, Ambion, Austin, TX) or a negative control miR (Invitrogen mirVana miRNA Mimic ...
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bioRxiv - Cell Biology 2020Quote: ... TaqMan Fast Universal PCR Mastermix and TaqMan miRNA Assays primers were used to detect miR-23a-3p and miR-10b-5p (QuantStudio 12K Flex, Thermo Fisher Scientific). All reactions were run in triplicate and results were expressed as 2−ΔΔCt ...
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bioRxiv - Genomics 2024Quote: ... and miR-1 (Life Technologies, assay name: hsa-miR-1, cat# 4427975) following the manufacturer’s recommendations (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... and hsa-miR-182 (Thermo Fisher Scientific #002334). Relative expression of miRNAs analyzed in triplicates were normalized to the levels of Z30 and expressed as 2−ΔΔCt.
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using Taqman probes and primer sets (Applied Biosystems) specific for CD36 (assay ID Mm00432398_m1) ...
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bioRxiv - Molecular Biology 2020Quote: ... miR-200a-3p (MC10991) and negative control miRNA (Ambion) were used for these transfections.
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bioRxiv - Molecular Biology 2019Quote: ... miRNA mimics and the miR-100 isoforms miR-100-5p and miR-100-3p (all from Life Technologies) using 15 pmol PepMute reagent (SignaGen Labs ...
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bioRxiv - Neuroscience 2024Quote: ... Primers used were: rno-miR-155-5p (RT:002571, ThermoFisher Scientific), and rno-miR-674-3p (RT:001956 ...
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bioRxiv - Neuroscience 2022Quote: ... hsa-miR-10 (Applied Biosystems miRNA assay ID 000387), hsa-miR-129 (Applied Biosystems miRNA assay ID 000590 ...
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bioRxiv - Neuroscience 2022Quote: ... hsa-miR-129 (Applied Biosystems miRNA assay ID 000590) and hsa-miR-132 (Applied Biosystems miRNA assay ID 000457).
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bioRxiv - Genomics 2022Quote: ... and hsa-miR-152 (Life Technologies, Assay ID: 000475). Gene expression was assayed with use of the following probes ...
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bioRxiv - Neuroscience 2023Quote: ... clones were hsa-miR-21 (Thermo Fisher Scientific, #0003970), Let-7a (Thermo Fisher Scientific # 000377) ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218 (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... and hsa-miR-132 (Applied Biosystems miRNA assay ID 000457).
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bioRxiv - Molecular Biology 2019Quote: ... We also measured miR-375-3p (Thermo Fisher Assay ID #00564), which was not included on the array ...
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bioRxiv - Bioengineering 2023Quote: ... synthetic cel-miR-39-3p (Thermo Fisher Scientific, Waltham, MA, USA) was added to samples during the cell/MP lysis step as a spike-in control ...
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bioRxiv - Neuroscience 2021Quote: ... The probes hsa-miR-499a-5p (Alexa Fluor 546; Thermo Fisher) and CamK2 (Alexa Fluor 488 ...
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bioRxiv - Molecular Biology 2019Quote: ... 10-nM mirVana hsa-miR-21 specific inhibitor (Thermo Fisher Scientific), or mirVana miRNA inhibitor Negative Control (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... or miR-544-3p inhibitor (50 nM; Thermo Fisher Scientific, Waltham, MA), or negative control mimic (50 nM ...