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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Zika Virus NS1 Proteins Uganda Suriname Strains Duo Pack since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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bioRxiv - Microbiology 2024Quote: ... NS1 proteins were detected using DENV2 NS1 polyclonal antibody (1:1,000 ThermoFisher Scientific) with overnight incubation at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... and the expression plasmid encoding the GP gene of an EBOV Makona strain (GenBank accession no. KJ660346) or BDBV Uganda strain (GenBank accession no. KR063673) at a 4:1 ratio by lipofectamine 3000 (Thermo Fisher). After 48-72 h ...
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bioRxiv - Microbiology 2020Quote: Zika virus RNA yields were assessed using real-time PCR on a 7500 Fast Real-Time PCR instrument (Thermo Scientific, Poland). ZIKV cDNA was amplified in a reaction mixture containing 1× TaqMan Universal PCR Master Mix (RT-PCR mix ...
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bioRxiv - Immunology 2023Quote: ... and H1N1/2009 influenza virus strains were prepared by overnight incubation of the virus strains with 0.1% v/v β propiolactone (Acros Organics, Geel, Belgium) under continuous rotation at 4°C ...
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bioRxiv - Immunology 2021Quote: ... NS1 (Invitrogen Cat #PIPA532243, 1:1000), NP (BioRad Cat#MCA400 ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with pcineo plasmids (0.4 μg) carrying the RSV codon-optimised NS1 or FLAG-NS1 WT or mutant constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Starter Pack (Invitrogen, F10471) for 30 mins at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... using King Fischer Duo (Thermo Fisher Scientific) or ReliaPrep RNA Miniprep Systems (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Immunostaining of NS1 and TRIM25 was realized by using the following antibody combination: mouse anti-NS1/goat anti-mouse Alexa 488 (ThermoFisher Scientific) and rabbit anti-TRIM25 (abcam)/goat anti-rabbit Atto647N (sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... alongside Chameleon Duo Protein Ladder (3 μL/lane; LiCOR, 928-60000) or PageRuler Protein Ladder (5 μL/lane; Thermo Scientific, 26616) and run in pre-chilled 1X MOPS Buffer (Thermo ...
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bioRxiv - Genetics 2022Quote: ... alongside Chameleon Duo Protein Ladder (3 μL/lane; LiCOR, 928-60000) or PageRuler Protein Ladder (5 μL/lane; Thermo Scientific, 26616) and run in pre-chilled 1X MOPS Buffer (Thermo ...
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bioRxiv - Biophysics 2023Quote: ... and nitrocellulose packs (Thermofisher IB23001). Nitrocellulose membranes were removed and replaced with PVDF membranes (BioRad #1620177 ...
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bioRxiv - Cancer Biology 2022Quote: ... Li-COR chameleon duo pre-stained protein ladder (Li-COR, 928-60000) along with 100 μg of protein combined with loading buffer (Thermo Scientific, NP0007) were loaded onto a 4%-12% Bis-Tris gel (Thermo Scientific ...
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bioRxiv - Genetics 2021Quote: ... Quantifiler® Duo DNA Quantification kit (ThermoFisher Scientific) was used to quantify extracts according to the manufacturer’s recommended protocols using the Applied Biosystems’ 7500 real-time PCR instrument (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... with KingFisher Duo Prime Purification System (Thermo Scientific). Total RNA was converted to cDNA using High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Using a KingFisher Duo Prime (Thermo Scientific 5400110) in a 96-well deepwell plate (Thermo Scientific 95040452 ...
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bioRxiv - Cell Biology 2021Quote: ... coli (K-12 strain), and Staphylococcus aureus (Wood strain, without protein A) (Molecular Probes), were washed 3 times with PBS by centrifugation and sonicated for 3 times at 50 KHz for 20 s ...
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bioRxiv - Immunology 2020Quote: ... aureus bioparticles (Wood strain without protein A) (Thermofisher) were resuspended in PBS with 2 mM sodium azide and 4–20×106 bioparticles were injected directly into tumors ...
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bioRxiv - Microbiology 2023Quote: ... anti-NS1 and anti-NS2 (all 1:2000, Thermo Fisher). Membranes were washed in PBS-T and incubated with fluorescent secondary antibody (anti-mouse or anti-rabbit conjugated to AlexaFluor488 or 647 ...
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bioRxiv - Cancer Biology 2019Quote: ... Pulldowns were performed in a KingFisher Duo (Thermo Scientific). Cleared lysates were incubated with protein G Mag Sepharose™ Xtra beads (GE Healthcare ...
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bioRxiv - Bioengineering 2019Quote: KingFisher™ Duo Prime Purification System (Thermo Fisher Scientific)
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bioRxiv - Developmental Biology 2020Quote: ... in a KingFisher Duo Prime system (Thermo Fisher Scientific). Samples were mixed with RNA stable (Biomatrica) ...
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bioRxiv - Microbiology 2020Quote: ... and a Kingfisher duo prime extraction machine (Thermo Scientific). SARS-CoV-2 RNA levels were measured by one-step quantitative reverse transcriptase PCR (qRT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... via a FAIMS Pro Duo ion source (Thermo Fisher). The instruments were operated in data-dependent acquisition (DDA ...
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bioRxiv - Immunology 2023Quote: ... via a FAIMS Pro Duo ion source (Thermo Fisher). The instrument was operated in data-dependent acquisition (DDA ...
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bioRxiv - Developmental Biology 2023Quote: ... in a KingFisher Duo Prime system (Thermo Fisher Scientific). Samples were mixed with RNA stable (Biomatrica) ...
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bioRxiv - Microbiology 2023Quote: ... Presence of each virus strain in the supernatant was determined by PCR using Taq polymerase (Thermo Scientific) and 20 μL reactions containing 1 μL of virus supernatant ...
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bioRxiv - Synthetic Biology 2020Quote: Proteins were expressed from E.coli BL21-AI strains (Invitrogen) carrying appropriate plasmids for expression of E.coli Gnd or E.coli RpiA (ribose-5-phosphate isomerase ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Vanquish Duo UHPLC System for Dual LC Workflows (Thermo Scientific); Waters BEH UPLC C18 column( 1.7 μm ...
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bioRxiv - Microbiology 2023Quote: ... equipped with a FAIMS Pro Duo interface (Thermo Fisher Scientific) operating with Xcalibur 4.6 and Tune 4.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The latter was performed in the National Laboratory of Research and Services in Peptides and Proteins using an LCQ DUO ESI ion trap (Thermo Finnigan) or QExactive Orbitrap (Thermo Scientific) spectrometers.
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bioRxiv - Biochemistry 2023Quote: The protein targets and beads-only control (B buffer replacing the protein) were screened in duplicate using a KingFisher Duo Prime (Thermo Scientific) in a 96-well deepwell plate (Thermo Scientific 95040452 ...
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bioRxiv - Molecular Biology 2023Quote: ... BL21(DE3) protein expression strain was obtained from Thermo Fisher Scientific.
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bioRxiv - Microbiology 2023Quote: ... then using the KingFisher™ Duo Prime (Thermo Scientific, Waltham, MA) and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit to extract from the pellets as specified in the manual (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher) (31 ...
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bioRxiv - Microbiology 2023Quote: Influenza A virus (IAV) strain A/WSN/33 [H1N1 subtype] was propagated in Madin-Darby Canine Kidney (MDCK) cells (ThermoFisher). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Immunology 2023Quote: ... The recombinant NS1 gene was cloned into the pTRC–His2c expression vector (Invitrogen) and expressed in the BirA-enzyme containing E ...
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bioRxiv - Evolutionary Biology 2022Quote: ... genomic DNA was extracted with KingFisher Duo magnetic particle processor (ThermoFisher Scientific) using the KingFisher Cell and Tissue DNA Kit ...
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bioRxiv - Cell Biology 2019Quote: ... equipped with a pack of oxygen-consuming reagent (ThermoFisher) and oxygen color indicators (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... stained with the Hema 3 Stat Pack (Fisher Scientific) and finally covered with coverslips mounted on Fluoromount-G (SouthernBiotech ...
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bioRxiv - Cancer Biology 2023Quote: ... Starter Pack was used per manufacturer’s instructions (Invitrogen, F10471). AR-C 118925XX was reconstituted in DMSO according to manufacturer’s instructions (Tocris ...
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bioRxiv - Immunology 2022Quote: ... or mouse anti-Hepatitis C virus Core protein (clone C7-50, Life Technologies) were visualised using IRDye 800CW Donkey anti-Rabbit IgG or Goat anti-Mouse IgG (LiCor) ...
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bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
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bioRxiv - Biochemistry 2023Quote: Protein samples were digested on-bead using multimode magnetic microparticles (MagReSyn® HILIC, ReSyn Biosciences) in a KingFisher Duo™ system (Thermo Fisher Scientific), as previously described (33 ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted using the KingFisher Duo Prime System (Thermo Fisher Scientific) together with a high performance RNA bead isolation kit (Molecular Biology Service ...
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bioRxiv - Plant Biology 2022Quote: ... Total proteome peptide pools were then generated using a KingFisher Duo (Thermo Scientific) automated sample preparation device as outlined by Leutert et al ...
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bioRxiv - Biochemistry 2022Quote: ... Automated RNase digestions were performed on a KingFisher Duo Prime system (Thermo Scientific) using BindIt™ software (version 4.0 ...
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bioRxiv - Zoology 2021Quote: ... with a manufacturer-designed protocol for the Kingfisher Duo Prime (ThermoFisher Scientific, #5400110) automated DNA purification system ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or an automated system (King Fisher Duo Prime System, Thermo Scientific, MA, USA) in combination with a magnetic bead-based method (Mag-Bind Blood & Tissue DNA HDQ kit ...