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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Yellow Fever Virus Envelope Protein Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The Yellow fever NS5 protein was codon-optimised using the algorithms provided by Invitrogen (Thermo Fisher, USA), and a FLAG-tag encoding sequence was added to the carboxyl-terminus ...
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bioRxiv - Molecular Biology 2020Quote: Genomic RNAs of PRRSV (porcine reproductive and respiratory syndrome virus) and CSFV (classic swine fever virus) were extracted following the TRIzol method (TRIzol reagent, Invitrogen, California, USA). Extracted RNA of PRRSV was examined using VDx PRRSv qRT-PCR (NA/EU dual ...
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bioRxiv - Microbiology 2019Quote: ... 0.35μg of each of 10 reovirus genes and 0.8μg C3P3 (T7 RNA Polymerase linked with (G4S)4 to NP8686 African Swine Fever Virus capping enzyme) were diluted in 50μl Opti-MEM (31985070, ThermoFisher Scientific). 11μl TransIT®-LT1 (MIR2300 ...
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bioRxiv - Immunology 2022Quote: ... or IgG1 Fc (human) protein (Thermo Fisher Scientific, Rockford, IL) was added to a final concentration of 10 mg/mL and samples were incubated with gentle mixing for 2 hours at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... upstream of a Yellow Fluorescence Protein (YFP) encoding gene and an HA tag using the Gateway cloning system (ThermoFisher Scientific). The resulting plasmids were verified by sequencing and mobilized into A ...
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bioRxiv - Microbiology 2023Quote: ... Strep-tag (NBP2-41073; Novusbio) or influenza virus hemagglutinin tag (2-2.2.14; Thermo Scientific). After washing with PBS with 1% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... along with psPAX2 and pMD2.G packaging and envelope virus using lipofecctamine 3000 (Invitrogen) in serum-free Opti-MEM media (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: The coding sequence of MHV-S Hemagglutinin Esterase inactive (S45A) (1218bp) fused to human IgG-Fc followed by a 6xHis tag in the mammalian expression vector pcDNA3.1(-) (Invitrogen) was kindly provided by Colin Parrish (MHV-S-HE_Fc_His6 ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
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bioRxiv - Immunology 2022Quote: ... after which cells were permeabilised (0.1% Triton X100) and stained with an antibody recognising dengue virus envelope protein (Invitrogen MA1-27093, for DENV serotypes 1-3), pan flavivirus envelope (Millipore MAB10216 ...
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bioRxiv - Immunology 2021Quote: ... residues 306–577 of MERS-CoV S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Immunology 2020Quote: ... or residues 319–591 of SARS-CoV-2 S with a C-terminal monomeric human IgG Fc-tag and an 8x HisTag (SARS-CoV-2 RBD-SD1) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Bioengineering 2020Quote: ... Goat anti-human Fc-HRP conjugate (Invitrogen) was used at a 1:10,000 dilution in blocking buffer for 1 h at RT ...
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bioRxiv - Immunology 2019Quote: ... The full length and truncation sequences were transferred into a Gateway compatible destination vector that includes an N terminal triple-epitope tag (S protein tag, FLAG epitope tag and Streptavidin-binding peptide tag) using the LR clonase enzyme kit (ThermoFisher). Plasmids were transfected into 293T cells using FuGENE transfection reagent (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... The Yellow Fluorescent Protein (YFP) coding sequence was PCR amplified (PHUSION, Thermofisher) and cloned in pBluescriptSK+ using EagI-XbaI sites provided by the primers ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Immunology 2021Quote: ... CellTrace Yellow (ThermoFisher) or CellTrace Red (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-RBD fused with Fc/His tag (S-RBD-His) was produced in 293T cells and purified with Protein A Agarose (Thermo Fisher Scientific). Recombinant human ACE2-ECD fused with Flag/His tag was produced in 293T cells and purified with anti-DYKDDDDK G1 Affinity Resin according to the manufacturer’s instructions (GenScript).
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bioRxiv - Systems Biology 2021Quote: ... anti-human FITC-IgG (Fc specific) (ThermoFisher Scientific) at a dilution of 1:400 and for 45 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... 100ug/mL anti-human IgG(Fc) (Life Technologies) was added to virus-Ab complexes and incubated for 30 min at 37°C prior to the addition of cells as described in (39) ...
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bioRxiv - Neuroscience 2022Quote: ... Flies were covered in yellow dust (Reactive Yellow 86, Fisher Scientific)15 and placed in a cylindrical custom designed chamber ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: Recombinant NRX1β-Fc and NRX1βΔHRD-Fc proteins were pre-immobilized with Protein G Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) in PBS overnight at 4 °C with constant agitation ...
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bioRxiv - Biophysics 2020Quote: ... and inserted into a human Fc expression vector (Invitrogen). The proteins were expressed in HEK293T cells ...
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bioRxiv - Cell Biology 2022Quote: ... yellow-green Fluospheres (ThermoFisher). Yellow-green Fluospheres show up in both channels and are used for registration of green and red images in conjunction with a custom MATLAB code ...
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bioRxiv - Immunology 2020Quote: ... pEco envelope was used to produce virus to infect murine T cells) using lipofectamine 2000 (Cat. No. 11668019, Invitrogen, Carlsbad, California, USA) on a 100□mm2 poly-D-lysine–coated plate (Corning ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Bioengineering 2022Quote: ... and primary human CD8+ T cells were stained with either Cell Trace Yellow (Thermo Fisher Scientific) or CFSE (Tonbo Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... WT human PRRT2 (gene ID: 112476) was synthesized as N-terminal HA tag fusion protein (Genewiz) in pcDNA3 vector (Thermofisher) and single point mutants were generated on this matrix by standard molecular biology techniques.
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bioRxiv - Cancer Biology 2023Quote: ... Human OTUD4 constructs were cloned without tag or with FLAG-tag into the expression plasmid pcDNA3.1 (Life technologies) using the oligos forward (BamHI ...
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bioRxiv - Biophysics 2021Quote: ... and both HRP-conjugated anti-mouse IgG Fc and anti-human IgG Fc were purchased from Invitrogen™ (Waltham ...
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bioRxiv - Microbiology 2021Quote: ... FXa-HRP conjugated anti-human Fc antibody (05-4220, Invitrogen) was used as a detecting antibody ...
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bioRxiv - Immunology 2021Quote: ... then incubated with human Fc receptor coupled to phycoerythrin (ThermoFisher). To set positive gates ...
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bioRxiv - Immunology 2021Quote: ... or specific for the Fc region of human IgG (Invitrogen), the Fc region of human IgM (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... or specific for the Fc region of human IgG (Invitrogen), the Fc region of human IgM (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2021Quote: ... live/dead yellow (ThermoFisher Scientific), anti-TCRβ-APC-eF780 (H57-597 ...
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bioRxiv - Immunology 2020Quote: ... live/dead yellow (ThermoFisher Scientific), anti-TCRβ-APC-eF780 (H57-597 ...
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bioRxiv - Cell Biology 2022Quote: ... or yellow-green FluoSpheres (Invitrogen, F8800 ...
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bioRxiv - Cell Biology 2022Quote: ... and yellow-green (Invitrogen, F8803) fluorescent microspheres were diluted 1:20000 in methanol ...
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bioRxiv - Immunology 2022Quote: ... CellTrace Yellow (Thermo Fisher, C34567); Hoechst (Thermo Fisher ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... Violet or Yellow (ThermoFisher Scientific) was used according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... and CellTrace Yellow from Invitrogen.
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bioRxiv - Immunology 2024Quote: ... or CellTrace Yellow (Invitrogen C34567) for 30 minutes at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... a 6XHis tag and SUMO protein expression plasmid (Invitrogen), with a TA cloning site ...
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bioRxiv - Molecular Biology 2020Quote: ... HA Tag antibody for SaCas9 protein (2-2.2.14, Thermofisher), rabbit anti-GFP (A11122 ...
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bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...