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Citations for Thermo Fisher :
1 - 50 of 5029 citations for UBE2G2 Human GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Purified recombinant human p38α (MAPK14, GST-tagged, Thermo Fisher Scientific, #PV3304), p38β (MAPK11 ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant GST-tagged kinase active human mTOR (Cat. PV4753, ThermoFisher) was used instead of the immunoprecitated mTORC1 ...
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bioRxiv - Developmental Biology 2023Quote: ... a GST antibody (GST Tag Monoclonal Antibody, ThermoFisher) and a FLAG antibody (anti-Flag-HRP ...
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bioRxiv - Plant Biology 2022Quote: ... GST and GST-tagged SAMS were incubated with 40 μl GST magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C and then these beads were washed three times with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... GST and GST-UAB were purified from BL-21 cells with GST agarose (Thermo Scientific, PI20211) following the manufacture’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... GST(rabbit, Invitrogen), GFP (rabbit ...
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bioRxiv - Cell Biology 2021Quote: ... GST (Mouse, Invitrogen), GM130 (Mouse ...
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bioRxiv - Plant Biology 2021Quote: ... Detection of GST- and MBP-fused proteins was performed with anti-GST (Invitrogen) and anti-MBP antibodies (NEB) ...
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bioRxiv - Cell Biology 2019Quote: Recombinant GST and GST-tagged EB1 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.7 and induced with 1 mM IPTG for 5.5 h at 25°C ...
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bioRxiv - Plant Biology 2021Quote: ... and GST-△F-box-AFF1 proteins were immobilized on GST beads (Glutathione Agarose; ThermoFisher). Immobilized agarose beads containing 2 μg GST ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids encoding GST-YidC1CT or GST-YidC2CT were transformed into BL21 Star™ (ThermoFisher). GST-YidC1CT expression was induced with 0.5 mM isopropyl-ß-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged RASSF5 (GST-RASSF5) sequence was transferred into the pDEST15 expression vector (Thermofisher), and then expressed and purified from BL21-A1cells ...
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bioRxiv - Cell Biology 2023Quote: ... GST-TAF3 PHD proteins were purified using the Pierce GST Spin Purification Kit (ThermoFisher) and dialyzed against 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... or pT7CFE1-NHIS-GST (ThermoFisher) vectors for expression as N-terminal GST fusion proteins (Collaboration 2016) ...
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bioRxiv - Neuroscience 2020Quote: ... GST-AAK1 (1-501aa) (Thermofisher) with or without GST-AP2M1-WT or T156A protein were subjected to a kinase reaction in kinase buffer (20 mM HEPES pH=7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... GST (Mouse, Invitrogen, MA4-004). Rabbit phospho-Septin7 (pT426 ...
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bioRxiv - Microbiology 2023Quote: ... anti-GST (Invitrogen A-5800), anti-HA (Sigma H6908) ...
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bioRxiv - Cell Biology 2023Quote: ... before anti-GST antibody (Invitrogen) binding and detection as described above.
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bioRxiv - Genomics 2023Quote: ... Verification of a successful GST-tagged AP2 protein purification by GST-affinity purification (Thermo Scientific Pierce Glutathione Superflow Agarose beads ...
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bioRxiv - Cell Biology 2021Quote: Human ProtoArray® Microarray (v5.0) containing 9483 unique human proteins expressed as GST-fusion in insect cells were purchased from Invitrogen. Arrays were blocked in 4°C cold blocking buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... was used to generate plasmids GST::Cdc42N17 and GST::Cdc42N17using the Gateway™ Cloning System (Invitrogen). The GST::Crbıntra and GST::CrbıntraΔERUconstructs were provided by E ...
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bioRxiv - Plant Biology 2021Quote: ... 4 ng GST or GST-VDAL purified protein were loaded onto 10 kDa microspin columns (Ambion). After washed twice with 1 mL HS buffer (50 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... GST-tag (ThermoFisher Scientific #8-326), HIS-tag (Sigma-Aldrich H1029) ...
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bioRxiv - Neuroscience 2019Quote: GST-LRRK2 (aa 970-2527, Invitrogen) was incubated with His-HDAC6 (EMD Millipore ...
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bioRxiv - Genetics 2021Quote: ... and Anti-GST (Invitrogen, A-5800). Anti-Flag immunoprecipitations were carried out using magnetic beads pre-conjugated with the antibody (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... GST-14-3-3s constructs (Invitrogen) were transformed into Escherichia coli strain BL21 (DE3 ...
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bioRxiv - Molecular Biology 2022Quote: GST-tagged bait proteins were purified using Pierce GST Protein Interaction Pull-Down kit (Thermo-Fisher Scientific). To examine recombinant Pin1 phosphorylation ...
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bioRxiv - Plant Biology 2020Quote: ... The GST and GST-XIK fusion proteins were extracted with Pierce Immobilized Glutathione resin (Thermo Fisher Scientific) with the protein interaction buffer (20 mM Hepes-KOH pH7.2 ...
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bioRxiv - Cell Biology 2019Quote: ... GST or GST-EB1 (5 μg) were incubated with 20 μl glutathione agarose beads (16100; Thermo Scientific) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged TFs assayed in PBMs were detected either with Alexa488-conjugated anti-GST antibody (Invitrogen A-11131), or with a TF-specific PCRP antibody ...
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bioRxiv - Microbiology 2019Quote: ELISA was performed using anti-GST coated strips (Pierce™ Anti-GST Coated Clear Strip Plates, Thermo Scientific). A fixed amount of 10 pmol GST-tagged PA1217 was diluted in 200 µl PBS (137 mM NaCl ...
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bioRxiv - Immunology 2022Quote: Expression vectors for human His-NLRP1PYD (aa 1-92) and GST-NLRP1PYD were generated by Gateway cloning with destination vector pDEST17 (Thermo Fisher Scientific) and a customized destination vector based on pGEX-2T (a kind gift of Mikko Taipale ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal anti-GST (Thermo Fisher Scientific). HRP-conjugated secondary antibodies against mouse and rabbit were from Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2023Quote: ... and polyclonal anti-GST (Thermo Scientific, CAB4169) antibodies.
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bioRxiv - Microbiology 2023Quote: ... GST-RILP or GST proteins were purified by using a pre-equilibrated slurry of glutathione beads (Thermo Scientific, #16100) in TBS containing 1mM MgCl2 and washed three times in the same buffer containing 0.05% Triton X-100 ...
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bioRxiv - Cell Biology 2019Quote: ... heh1(735-834) was incubated with GST and GST-Chm7 in a dialysis cassette (3.5K Slide-A-Lyzer, Thermo Scientific) and the binding reaction was dialyzed overnight in a binding buffer (50 mM Tris pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... These cells were then permeabilised using 20 µM digitonin for 5 min and labelled with 8 µg/mL recombinant GST-2xFYVE (69) which was detected using a GST primary antibody (71-7500, Invitrogen) and a Goat anti-Rabbit Alex-aFluor647 secondary antibody (A-21245 ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins (GST-stop and GST-RILP) were transformed and isolated from BL21 E.coli and conjugated to Pierce Superflow GST agarose (Thermo Scientific). To probe for Rab7-GTP ...
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bioRxiv - Biochemistry 2020Quote: ... into pT7-N-His-GST (Thermo Fisher Scientific) or pET28a (EMD Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-GST (1:1,000, Invitrogen, PA1-982A), mouse anti-V5 (1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... The GST-Me31B clone in pFastBac1 (Thermo Fisher) has been described (Götze et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant active GST-TBK1 was from Thermo Fisher/ Life Technologies (#PV3504) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated with prewashed GST beads (Thermo Fisher) for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-GST-HRP (#MA4-004-HRP, Invitrogen)) were used at 1:10,000 for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... GST resin (cat no: 16101, Pierce, Thermofisher Scientific), Legionella antibody (ab
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bioRxiv - Cell Biology 2023Quote: ... or glutathione-S-transferase (GST) (Enzo Life Technologies) as a control protein ...
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bioRxiv - Microbiology 2021Quote: ... coli cells expressing WT GST-vWA and GST-vWA-C152S mutant proteins were resuspended in PBS supplemented with 2 mM TCEP (Thermo scientific), 0.01 mg/mL lysozyme from chicken egg (SIGMA) ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins (5 µg) along with GST alone were incubated with 10 µl of magnetic glutathione beads (Thermo Fisher Scientific) for one hour at RT ...