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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Yeast two-hybrid assay was performed using the ProQuest Two-Hybrid System (Invitrogen) by PEG mediated transformation following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The ProQuest Two-Hybrid System (Invitrogen) was used for the yeast two-hybrid assay ...
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bioRxiv - Microbiology 2021Quote: The ProQuest Two-Hybrid System (Invitrogen) was used ...
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bioRxiv - Cell Biology 2022Quote: Yeast two-hybrid analysis was performed using the ProQuest Two-Hybrid system (Thermo Fisher). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: Yeast two-hybrid (Y2H) interactions were characterized by employing the ProQuest Two-Hybrid System (Invitrogen Life Technologies) as reported in Cruz-Ramirez et al. ...
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bioRxiv - Developmental Biology 2022Quote: Yeast two-hybrid (Y2H) interactions were characterized by employing the ProQuest Two-Hybrid System (Invitrogen Life Technologies) as reported in Cruz-Ramirez et al. ...
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bioRxiv - Plant Biology 2023Quote: Yeast two-hybrid screening was performed according to the manufacturer’s instructions using the ProQuestTM Two-hybrid system (Invitrogen). AHL10 full length coding sequence was cloned into the entry vector pENTR-D-TOPO and recombined into the bait destination vector pDEST32 with GAL4 DNA binding domain (DBD) ...
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bioRxiv - Plant Biology 2024Quote: The yeast two-hybrid assay was performed according to previous publications using the ProQuest Two-Hybrid System (Invitrogen) following the manufacturer’s instructions52 ...
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bioRxiv - Biochemistry 2022Quote: ... Control plasmids from ProQuest™ two-hybrid system (Life Technologies). Co-transformation with empty prey vector was used as negative control ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed using ProQuest™ Two-Hybrid System with Gateway™ Technology (Thermo Fisher, Waltham, MA, USA). Arabidopsis ABI1 and PYR1 cDNAs cloned in pENTR-D-TOPO were introduced into pDEST22 and pDEST32 ...
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bioRxiv - Plant Biology 2019Quote: ... Directed yeast two-hybrid experiments were performed according to the ProQuest™ Two-Hybrid System with Gateway® Technology manual (Invitrogen). Full-length CMU1 and CMU2 along with a FRA1 C-terminal fragment (amino acids 616-1035 ...
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bioRxiv - Developmental Biology 2021Quote: ... Yeast two-hybrid interaction assays and color reactions were performed as described in the ProQuest Two-Hybrid System (Invitrogen #PQ10001-01, Carlsbad).
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bioRxiv - Immunology 2023Quote: ... Gateway® cloning-compatible vectors pDEST32 and pDEST22 (Invitrogen ProQuest yeast two-hybrid System) were used ...
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bioRxiv - Genetics 2022Quote: ... A forward Y2H library screen was performed using the ProQuest Two-Hybrid System (ThermoFisher, PQ1000101) to identify protein interactors of the C ...
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bioRxiv - Plant Biology 2024Quote: ... ATML1 fragments were transferred to pDEST32 bait and pDEST22 prey vectors (Proquest Two-Hybrid System, Invitrogen) using Gateway LR Clonase II Enzyme mix (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... Gateway cloning was used to transfer genes for HD-Zip III and IV TFs from pENTR223.1 clones to pDEST22 and pDEST32 destination vectors (ProQuest Two-Hybrid System, Invitrogen). The bait and prey plasmids were transformed into yeast strain MaV203 (Invitrogen) ...
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bioRxiv - Plant Biology 2022Quote: ... ATML1 cDNAs and the various deletion and missense mutations were transferred from pENTR/D-TOPO to pDEST32 bait and pDEST22 prey vectors (Proquest Two-Hybrid System, Invitrogen) using Gateway LR Clonase II Enzyme mix (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: Full-length wild-type and mutant cDNAs in pENTR/D-TOPO vectors were transferred to pDEST32 bait and pDEST22 prey vectors (Proquest Two-Hybrid System, Invitrogen) using Gateway LR Clonase II Enzyme mix (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... RNA was extracted from two pools of ovaries using a hybrid of Trizol (Ambion) and RNeasy mini kit (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... the MultiSite Gateway Three-Fragment Vector Construction Kit (Invitrogen) was used according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: The CloneMiner™ II cDNA library construction kit (Invitrogen) was employed to construct a cDNA library in P178 maize seedlings ...
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bioRxiv - Molecular Biology 2023Quote: ... A Cloneminer II cDNA Library Construction Kit (Invitrogen, Cat #A11180) was used to generate cDNA and clone it into plasmids for bacterial expression according to the specifications in the manual ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... The eight rice 14-3-3 genes cloned in the yeast two-hybrid vector pDEST22 (Invitrogen) were used from a previous study (Deb et al. ...
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bioRxiv - Plant Biology 2020Quote: ... At1g08710 was further subcloned in yeast two hybrid vector pDEST-GBKT7 using Gateway cloning technology (Invitrogen, CA). Cloning was confirmed by colony PCR and sequencing.
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bioRxiv - Plant Biology 2021Quote: ... Plasmids used for yeast two-hybrid or Co-IP were generated by gateway LR cloning (Life Technologies) using the ENTRY vectors described above and appropriate destination vectors listed in Suppl ...
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bioRxiv - Plant Biology 2022Quote: ... and subsequently cloned into pGILDA and pB42AD yeast-two-hybrid vectors (Clonetech) via LR Gateway Reactions (ThermoFisher). To introduce T653L and Y643* mutations into ARFd1 entry clone ...
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bioRxiv - Plant Biology 2023Quote: ... the MultiSite Gateway Three-Fragment Vector Construction Kit (Invitrogen; Ghent, Belgium) was used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Other reagents used for the construction of expression plasmids include the MultiSite Gateway Three-fragment Vector Construction Kit (Invitrogen Catalog number 12537- 023), DH5α competent cell (ThermoScientific catalog number 18265017) ...
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bioRxiv - Molecular Biology 2022Quote: All plasmids used in yeast two-hybrid assays were cloned with the Gateway® Gene Cloning Technology (Invitrogen) and transformed in the AH109 and Y187 haploid yeast strains (Clontech) ...
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bioRxiv - Plant Biology 2023Quote: For construct assembly the MultiSite Gateway Three-Fragment Vector Construction Kit (Invitrogen) was used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... sequencing library construction using Clontech SMARTER smRNA SEQ KIT (Fisher Scientific: NC1098027), rRNA depletion using subtraction oligo33 ...
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bioRxiv - Genetics 2023Quote: Constructs were cloned using the Multisite Gateway Three-Fragment Vector Construction System (Thermo Fisher Scientific) and destination vectors (ie ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Library construction was performed with the Ion Total RNA-seq kit (Life technologies) and the Ion XpressRNA kit (Life technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... was used for whole transcriptome library construction according to the Ion Total RNA-Seq Kit for the AB Library Builder™ System (Life Technologies, 4482416) protocol for barcoded libraries ...
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bioRxiv - Cell Biology 2019Quote: ... following the purification hybrid method from the ProBond purification system (Thermo Fisher Scientific). The purified recombinant protein was used to produce anti-CAP-D2 polyclonal antibodies in rabbit (Udo Conrad ...
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bioRxiv - Cancer Biology 2023Quote: ... A hybrid quadrupole Orbitrap (Q Exactive) MS system (Thermo Fisher Scientific, Bremen, Germany) was used with high energy collision dissociation (HCD ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of the xopX gene and its 14-3-3 protein binding motif mutants were cloned in the yeast two-hybrid vector pDEST32 (Invitrogen) using the Gateway cloning system (Invitrogen ...
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bioRxiv - Evolutionary Biology 2019Quote: All genes were transferred from the above generated GatewayTM compatible entry clones into the respective Yeast Two-Hybrid vectors with the GatewayTM LR Clonase II enzyme mix (Invitrogen). The codon-optimized sequences corresponding to mature proteins (without signal peptides ...
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bioRxiv - Synthetic Biology 2021Quote: All yeast strains were derived from parental strains Fy251 [American Type Culture Collection (ATCC) 96098] or the two-hybrid strain MaV20330 (Invitrogen). Yeast transformations were carried out using the lithium acetate method48 ...
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bioRxiv - Cell Biology 2022Quote: ... The siRNAs used to test the role of spatacsin interactors identified by the two-hybrid screen were Silencer Select siRNAs from ThermoFisher and are listed in Supplementary Table 3.
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bioRxiv - Molecular Biology 2023Quote: ... Depletion of rRNA-DNA hybrids was achieved by two 15 min incubation periods with streptavidin-coated magnetic Dynabeads MyOne C1 (0.45 mg ThermoFisher Scientific) in 0.25 mM EDTA ...
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bioRxiv - Cell Biology 2024Quote: Molecular cloning was mostly conducted by Multisite Gateway Three-Fragment Vector Construction system (Invitrogen, #12537-023). The plasmid pYW1693 (Pvha-6::fat-7::eGFP_let-858_3’-UTR ...
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bioRxiv - Cell Biology 2022Quote: ... nano HPLC system coupled to a hybrid Orbitrap Elite ETD (Thermo Scientific, Waltham, MA) mass spectrometer ...
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bioRxiv - Cancer Biology 2023Quote: ... A hybrid quadrupole Orbitrap (Q Exactive plus) MS system (Thermo Fisher Scientific, Bremen, Germany) was used with high energy collision dissociation (HCD ...
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bioRxiv - Cancer Biology 2023Quote: The hybrid quadrupole Orbitrap (Q Exactive Plus) MS system (Thermo Fisher Scientific, Bremen, Germany) was interfaced with an automated Easy-nLC 1200 system (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-seq libraries construction was performed with the Ion Total RNAseq Kit v2 (Thermo Fisher), using Poly(A ...
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bioRxiv - Molecular Biology 2020Quote: ... and the entry library was prepared using CloneMiner™ II cDNA library construction kit (Invitrogen) using Gateway technology ...
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bioRxiv - Cancer Biology 2024Quote: siRNA for mouse-IGF1R was constructed in vitro using the Silencer siRNA Construction Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Using the Gateway® two-step recombination system (Life Technologies), the fragment was first inserted into pDONR221 in the BP reaction and then incorporated into a modified pBID-UASC-GV vector (Wang et al. ...
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bioRxiv - Cell Biology 2019Quote: ... For feeder free systems we used two matrices Geltrex (ThermoFisher) and Vitronectin (Stem cell Technologies) ...