Labshake search
Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Toxoplasma gondii GRA7 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: Toxoplasma gondii tachyzoites were amplified in human foreskin fibroblasts (HFFs, ATCC) in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 5% of Fetal Bovine Serum (FCS ...
-
bioRxiv - Immunology 2020Quote: Tachyzoites of Toxoplasma gondii strains were passaged in ‘Toxo medium’ [4.5 g/liter D-glucose in DMEM with GlutaMAX (Gibco, cat#10566024), 1% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2024Quote: ... gondii (Thermo Scientific PA17252) with 1:750 goat anti-rabbit Alexa 594 secondary antibody (Thermo Scientific A-11005) ...
-
bioRxiv - Microbiology 2022Quote: ... gondii ME49 proteins (ToxoDB release 38) using Proteome Discoverer v2.5 (Thermo Fisher Scientific) with the Sequest HT search engine ...
-
bioRxiv - Microbiology 2022Quote: ... gondii polyclonal antibody (Invitrogen PA17252) at 1:10,000 ...
-
bioRxiv - Microbiology 2022Quote: ... gondii polyclonal antibody (Invitrogen PA17252) at 1:10,000 and Biotinylated-DBA at 1:500 ...
-
bioRxiv - Immunology 2023Quote: ... ⍺-Toxoplasma polyclonal (Thermo Fisher Scientific, PA1-7253, 1:500 dilution), ⍺-TgSAG1 (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... and a polyclonal rabbit anti-Toxoplasma antibody (Thermo Fisher Scientific, PA17252, 1:5000) overnight at 4° C ...
-
bioRxiv - Microbiology 2020Quote: ... gondii tachyzoites were loaded on 8-16% TGX gels (Life Technologies). After SDS-PAGE separation ...
-
bioRxiv - Pathology 2021Quote: ... gondii using 5X MagMAX viral Isolation kit (Applied Biosystems AMB1836-5). Primers and probes for real-time PCR were designed according to the protocol of De Craeye et al ...
-
bioRxiv - Microbiology 2023Quote: ... gondii strains were cultured in Lab-Tek chamber slides (177437, Nunc). Infected cells incubated or not with the chelator were then chemically fixed for 2h at room temperature using the fixation solution (2.5% glutaraldehyde in 0.1 M Cacodylate buffer ...
-
bioRxiv - Immunology 2020Quote: ... gondii PVM was stained with polyclonal rabbit anti-GRA7 primary antibody (gift from John Boothroyd, Stanford University) and Alexa Fluor 488 anti-rabbit IgG (Life Technologies) at 1:3000 dilution ...
-
bioRxiv - Microbiology 2019Quote: ... gondii cyst and was visualized using Alexaflour-594 conjugated streptavidin (Thermo Scientific). Cysts were counted by microscopy to calculate cyst burden for each brain.
-
bioRxiv - Immunology 2020Quote: ... gondii tachyzoites in T cell medium (RPMI 1640 with GlutaMAX (Gibco, cat#61870127), 20% heat-inactivated FBS (Omega Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... gondii parasites were grown in human foreskin fibroblasts (HFFs) maintained in DMEM (GIBCO) supplemented with 3% inactivated fetal calf serum (IFS ...
-
bioRxiv - Microbiology 2022Quote: ... gondii parasites were grown in human foreskin fibroblasts (HFFs) maintained in DMEM (GIBCO) supplemented with 3% heat-inactivated newborn calf serum (Millipore Sigma) ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were then permeabilized and incubated with 1:1000 dilution of rabbit anti-Toxoplasma primary antibody (Invitrogen PA17252) followed by a final incubation with secondary antibodies goat anti-mouse Alexa Fluor 488 (1:5000 ...
-
bioRxiv - Cell Biology 2022Quote: The stimulated Toxoplasma tachyzoites and the purified Plasmodium merozoites were imaged using a Talos Arctica electron microscope (Thermo Fisher) equipped with a field emission gun operated at 200kV ...
-
bioRxiv - Cell Biology 2024Quote: ... gondii tachyzoites were cultured in human foreskin fibroblasts in Dulbecco’s modified Eagle’s medium (Gibco: 11960-051) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Microbiology 2022Quote: ... parasites were resuspended in warm PBS (with BIPPO in the case of Toxoplasma) and sedimented on coverslips coated with poly-lysine (Gibco). The excess liquid was then discarded and the coverslips were incubated for 5h at 37°C in a mix of 0.7% formaldehyde / 1% acrylamide ...
-
bioRxiv - Immunology 2021Quote: ... gondii-challenged DCs were plated on coverslips coated with bovine collagen I (1 mg/ml, Life Technologies). After fixation (4% PFA ...
-
bioRxiv - Microbiology 2023Quote: ... gondii parasites were grown in human foreskin fibroblasts (HFFs, ATCC SRC-1041) maintained in DMEM (GIBCO 11965118) supplemented with 3% inactivated fetal calf serum and 10 µg/mL gentamicin (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... gondii tachyzoites were maintained by serial passage in HFFs in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher) containing 10% fetal bovine serum (FBS) ...
-
bioRxiv - Microbiology 2024Quote: ... gondii polyclonal (in-house) with 1:750 goat anti-mouse Alexa 594 secondary antibody (Thermo Scientific 11-005). Secondary antibodies and DAPI were incubated 1 hour at RT ...
-
bioRxiv - Bioengineering 2020Quote: ... gondii GT1 T NCBI Genome assembly version 7/19/2013 genome references with ERCC sequences (obtained from ThermoFisher website). The raw fastq files from sequencing are aligned to the concatenated genomes with STAR aligner (version 2.6.0c ...
-
bioRxiv - Immunology 2020Quote: ... qPCR for parasite burden was conducted using toxoplasma specific primers: (forward) 5’-TCCCCTCTGCTGGCGAAAAGT-3’ and (reverse) 5’-AGCGTTCGTGGTCAACTATCGATT G-3’ and Power SYBR Green master mix (Applied Biosystems, CA, USA). The qPCR condition settings were ...
-
bioRxiv - Immunology 2023Quote: ... gondii 529-bp repeat element (RE) compared with mouse β-actin as described previously83 using Taqman probes (Thermo Fisher Scientific). Water or uninfected animals were used as a negative control ...
-
bioRxiv - Microbiology 2023Quote: ... gondii growth were determined in HFF cell cultures using 96-well black plates (Optical-Bottom Plates with Polymer Base, ThermoFisher Scientific). Sulfadiazine (positive control ...
-
bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
-
bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
-
bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
-
bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
-
bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
-
bioRxiv - Genomics 2020Quote: ... and Protein A or Protein G Dynabeads (Invitrogen). After incubation ...
-
bioRxiv - Neuroscience 2019Quote: ... Protein reagent (Thermo Scientific PierceTM BCA Protein assay) was added to the supernatant and the standards and incubated for 30 min at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... or with Dynabeads protein A/protein G (Thermofisher) combined with Abatacept ...
-
bioRxiv - Biochemistry 2021Quote: ... protein marker (NativeMark Unstained protein standard, LC0725, ThermoFisher) was diluted 50x in the working buffer (40 mM HEPES pH 7.5) ...
-
bioRxiv - Immunology 2020Quote: ... protein A and protein G magnetic beads (Invitrogen) were added to capture the antibody-chromatin complexes ...
-
bioRxiv - Immunology 2021Quote: ... For protein purification protein G dynabeads (Thermo Fisher), coupled with 2.5 µg of antibody (Table S8 ...
-
bioRxiv - Neuroscience 2022Quote: ... Magentic dynabeads Protein G or Protein A (Invitrogen) were washed with 0.05% Triton X-100 in PBS ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein A and Protein G Dynabeads (Thermofisher Scientific) were washed twice with IP dilution buffer (150 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Plant Biology 2022Quote: ... Magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for two hours ...
-
bioRxiv - Plant Biology 2022Quote: ... Magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours ...
-
bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen, Waltham, MA, USA). 5 µg of each protein fraction was added to 1x loading buffer (250 mM Tris pH 6.8 ...
-
bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...