Labshake search
Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Tick Borne Encephalitis Virus Lysate Strain Moscow B 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: Total RNA was isolated from the eggs at 24 hpo from a wild-type strain (p50T) and three b-4 mutant strains (e36, e37, and No. 903) using TRIzol (Invitrogen). Reverse transcription was then performed using the oligo (dT ...
-
bioRxiv - Immunology 2023Quote: ... and H1N1/2009 influenza virus strains were prepared by overnight incubation of the virus strains with 0.1% v/v β propiolactone (Acros Organics, Geel, Belgium) under continuous rotation at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... coli (type B cells, ATCC 11303 strain, 14380, Affymetrix) DNA was fragmented by a limited SauIIIA or AluI digest ...
-
bioRxiv - Biochemistry 2021Quote: ... coli B strain BL21(DE3) (Invitrogen catalog number C600003) was used for all experiments and E ...
-
bioRxiv - Microbiology 2020Quote: ... 100μg of whole cell lysate from each strain was run on NuPage 4-12% bis-tris protein gel (Thermo Scientific) at 120V for 3hours ...
-
bioRxiv - Microbiology 2020Quote: ... N-term B virus mut) using a Neon® Transfection System (Invitrogen) under the recommended electroporation condition (1350 V ...
-
bioRxiv - Neuroscience 2021Quote: ... 4% B-27 supplement (Gibco), and 2 mM Glutamax (Gibco) ...
-
bioRxiv - Neuroscience 2022Quote: ... virus was mixed with 4% fast green (Invitrogen) dissolved in saline and injected using a glass micropipette at a rate of 100 nl/min ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Lysate from a strain containing empty pET51b+ vector (Invitrogen, Waltham MA) was analyzed as negative control for background endogenous transacylation by E ...
-
bioRxiv - Developmental Biology 2019Quote: ... 4 mL 50X B-27 without Vitamin B (Gibco 12587010) per 200 mL of DMEM (Invitrogen 11960-051 ...
-
bioRxiv - Microbiology 2021Quote: ... Immobilised virus was fixed with 4% formaldehyde (Thermo Scientific) in phosphate buffered saline (PBS ...
-
bioRxiv - Immunology 2022Quote: ... containing 4% B-27 (Life Technologies), 30 μM ascorbic acid (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4% B-27 supplement (Thermofisher Scientific), 30 µM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Abelson virus-transformed pre-B cells were maintained in DMEM (Thermo Fisher, 11960-077) supplemented with 10% FBS ...
-
bioRxiv - Microbiology 2023Quote: ... Virus pellets and cell lysates were dissolved in radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific) containing protease inhibitors ...
-
bioRxiv - Plant Biology 2019Quote: ... The transformed fungal strains were selected with 300 μg.mL−1 hygromycin B (Invitrogen) and were later maintained under 150 μg.mL−1 hygromycin B selection pressure on modified P5 medium ...
-
bioRxiv - Microbiology 2019Quote: ... The plasmids used in the control reactions were generated by segment-specific RT-PCR from clinical samples of B/Victoria and B/Yamagata strains from the 2012-2013 season followed by gel extraction and TOPO-TA cloning (Invitrogen). The sequence of each plasmid was determined by Sanger sequencing ...
-
bioRxiv - Immunology 2020Quote: ... and 4 μg/ml amphotericin B (Gibco), was added to each well ...
-
bioRxiv - Bioengineering 2022Quote: ... supplemented with 4% B-27 (Gibco, 17504044) and 2 mM Glutamax (Gibco ...
-
bioRxiv - Bioengineering 2022Quote: ... supplemented with 4% B-27 (Gibco, 17504044) and 2 mM Glutamax (Gibco ...
-
bioRxiv - Molecular Biology 2021Quote: Abelson virus-transformed pre-B cell lines were maintained in DMEM (Thermo Fisher #11960-077) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Microbiology 2023Quote: ... Presence of each virus strain in the supernatant was determined by PCR using Taq polymerase (Thermo Scientific) and 20 μL reactions containing 1 μL of virus supernatant ...
-
bioRxiv - Biophysics 2023Quote: ... The precipitated bacteria were lysed with B-PER lysate solution (Thermo Scientific), and HisPur Ni-NTA agarose beads were added to pull down the 6xHis-tagged protein ...
-
bioRxiv - Microbiology 2023Quote: ... fumigatus maiA mutant strains were performed using Hygromycin B (Thermo Fisher, Waltham, MA, USA) as a selection marker ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were infected with virus particles and selected with hygromycin B (200 µg/ml, Invitrogen). For single clone selection ...
-
bioRxiv - Cell Biology 2022Quote: RNA was isolated from virus-containing medium or cell lysates using TRIzol LS (ThermoFisher Scientific, #10296010) according to the manufacturer’s instructions and stored at −80°C for RT-qPCR ...
-
The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... The lysate was mixed at room temperature with 20 mL B-PER (ThermoFisher) containing 10 mg lysozyme ...
-
bioRxiv - Pathology 2019Quote: ... 4% Penicillin/Streptomycin and 2% Amphotericin B (Gibco, UK). Lung lobes were separated and sliced in chilled HBSS buffer at a thickness of 250μm using the VT1200S Vibratome (Leica ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysates were further treated with 33% (v/v) B-PERII (ThermoFisher Scientific, UK) and 0.5% (v/v ...
-
bioRxiv - Microbiology 2021Quote: ... Protein lysates were quantified using BCA protein reagents A & B (Thermo Scientific, #23223, 23224) and 20 μg of protein lysates from each sample were denatured in 4X LDS sample buffer followed by separation on 4–12% gradient SDS PAGE polyacrylamide gel (Genscript ...
-
bioRxiv - Microbiology 2022Quote: ... The lysates were further treated with 33% (v/v) B-PERII (ThermoFisher Scientific, UK) and 0.5% (v/v ...
-
bioRxiv - Microbiology 2021Quote: ... The spike of the SARS-CoV-2.SctΔ19 B.1.617.2 (delta) virus was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the full protein sequence of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... C and S genes of the Hepatitis B virus (accession number: KY00323035) was ordered from GeneArt (ThermoFisher Scientific) and is available from Addgene (Addgene # 179155).
-
bioRxiv - Microbiology 2023Quote: Influenza A virus (IAV) strain A/WSN/33 [H1N1 subtype] was propagated in Madin-Darby Canine Kidney (MDCK) cells (ThermoFisher). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Microbiology 2023Quote: ... The cell and virus lysates were Western blotted and probed with a goat polyclonal anti-gp120 antibody (Invitrogen), the 4E10 anti-gp41 antibody (NIH HIV Reagent Program ...
-
bioRxiv - Neuroscience 2023Quote: ... We then cultured these variants and prepared clarified bacterial lysate using B-PER (Thermo Scientific). We measured the fluorescence spectra in the absence of Ca2+ (EGTA ...
-
bioRxiv - Cell Biology 2022Quote: Lysates were run on 4-12% polyacrylamide gels (NuPage, Invitrogen) for 50 minutes at 180V and transferred to polyvinylidene difluoride (Immobilon-FL ...
-
bioRxiv - Physiology 2020Quote: ... Protein lysates were separated using 4-12% SDS-PAGE (Invitrogen) and transferred to nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysates were run in 4-12 % gradient gels (NuPAGE, ThermoFisher) and proteins were transferred to a Nitrocellulose membrane for further analysis ...
-
bioRxiv - Neuroscience 2022Quote: ... lysates were incubated with 4 U/ml Turbo DNase (Invitrogen) and 0.2 to 0.002 units/ml of RNase I (Invitrogen) ...
-
bioRxiv - Biochemistry 2022Quote: Lysates were run on 4-12% polyacrylamide gels (NuPage, Invitrogen) for 50 minutes at 180V and transferred to polyvinylidene difluoride (Immobilon-FL ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were run on 4-12% Bis-Tris gel (Invitrogen), transferred to PVDF ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein lysates were analyzed on 4–12% NuPAGE gels (Invitrogen), transferred to PVDF membranes ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated with NuPAGE 4× LDS buffer (Invitrogen NP0008) with 50 mM dithiothreitol (DTT ...
-
bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
-
bioRxiv - Neuroscience 2020Quote: ... Mice were placed in a stereotaxic apparatus to receive bilateral infusions of the virus or 0.5% (w/v) cholera toxin subunit B (CTb) Alexa Fluor 555 Conjugate (Invitrogen) into the dorsal striatum (+0.1mm antero-posterior ...
-
bioRxiv - Microbiology 2023Quote: ... The concentration of proteins in tick EVs was determined using the BCA assay (Thermo Scientific), following the manufacturer’s procedure.
-
bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
-
bioRxiv - Neuroscience 2021Quote: ... The lysates were run on 4-12% SDS PAGE gels (Invitrogen), and dry transferred to 0.22 uM PVDF membranes ...
-
bioRxiv - Microbiology 2019Quote: ... 10μg total lysates were analysed by 4-12% SDS-PAGE (Invitrogen) and antibodies against MNV ...