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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Recombinant Bd37 (rBd37) protein was expressed as thioredoxin fusion protein from the pBAD/Thio-TOPO vector (Thermo Fisher Scientific), which encodes thioredoxin and histidine-tag at N-terminus and C-terminus of multi cloning site ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant human thioredoxin 1 (hTrx) was purchased from ThermoFisher. The plasmid pET-TRSter for heterologous expression of human thioredoxin reductase (hTrxR ...
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bioRxiv - Microbiology 2021Quote: ... NPC1 C domain and NPC1 I domain) and the viral protein constructs were co-transfected using Lipofectamine 2000 (Invitrogen) following the manufacture instructions.
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bioRxiv - Biochemistry 2020Quote: ... different domains or subunits of the Spike protein and the extracellular domain of ACE2 were produced in Expi293F cells (Thermo Fisher Scientific). Expi293F cells were cultured at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Co-purification of 6His-tagged PGC-1α protein domains was detected by western blot using anti-6His antibody (clone His.H8, ThermoFisher Scientific MA1-21315).
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bioRxiv - Immunology 2022Quote: ... The supernatant containing recombinant antibody was incubated with protein A resin (ThermoFisher) overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... using 3–5 µg of primary antibody against each protein and protein-G magnetic Dynabeads (10003D, Invitrogen) and incubated overnight at 4°C on rotation ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Immunology 2022Quote: ... Thioredoxin-fused peptides were chemically biotinylated with EZ-Link Sulfo-NHS-LC-biotin (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... “Protein extracted” with T7 tag antibodies were incubated with protein A containing magnetic beads (Dyna beads, Thermo fisher) for 2 hours at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Volumes containing 10 µg of protein were resolved using SDS-PAGE (NuPAGE 3-12% gels, Life Technologies or 10% acrylamide Tris-Glycine ...
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bioRxiv - Immunology 2022Quote: Expression plasmids encoding His-tagged SARS-CoV-2 RBD (residues 319 to 541 of SARS-CoV-2 S protein) or S protein (with a C-terminal trimerization domain) were cloned into pCEP4 vector and transfected into Expi293F (ThermoFisher Scientific) and the proteins expressed for 7 days at 37°C (Rouet et al. ...
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bioRxiv - Plant Biology 2020Quote: ... A Y2H prey library of Arabidopsis NTL proteins fused to the GAL4 activation domain (pDEST22; Invitrogen) was similarly created and transformed into the opposite yeast mating strain ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples containing 15 µg of protein were separated on 3–12% Bis-Tris Novex NativePAGE gel (Life Technologies). The relative levels of the assembled respiratory complexes I-V were assessed by western blot with following antibodies ...
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bioRxiv - Biochemistry 2024Quote: Construct containing Avi-tagged HormR/GAIN domains of ADGRL3 was expressed in High Five cells (Thermo Fisher, B85502) grown in Insect-Xpress medium (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... containing 3 mL buffer E (Thermo Fisher). Transfection conditions were adapted to CEM cells at 1230 V ...
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bioRxiv - Genomics 2019Quote: ... Soluble material was incubated with 3-5 μg of antibody bound to 50 μl protein A or protein G Dynabeads (Invitrogen) and incubated overnight at 4 °C ...
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bioRxiv - Genetics 2023Quote: ... The sample containing the antibody-chromatin complex was incubated with Dynabeads Protein G (Thermo Fisher) for H3K9me2 or Dynabeads M-280 Sheep anti-Mouse IgG (Thermo Fisher ...
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bioRxiv - Genetics 2020Quote: ... 3-Phosphoglycerate kinase (Pgk1) protein was detected with anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) and HSP90 protein was detected with a mouse anti-HSP90 monoclonal antibody (1:2000 ...
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bioRxiv - Genetics 2020Quote: ... 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal antibody generated against the C-terminal domain of N-cadherin (Invitrogen; 1:500); anti-CRB1 (a kind gift of Dr ...
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Microbiology 2021Quote: ... The antibody-containing supernatant was collected and purified using protein G spin columns (Thermo Fisher Scientific). Eluted purified IgG were dialyzed in PBS using a dialysis cassette (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The Xanthomonas thioredoxin A (TrxA, NZ_CP058243) sequence was synthesized by GeneScript and subcloned into the pDEST15 vector (ThermoFisher Scientific) to create pDEST-GST-TrxA ...
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bioRxiv - Microbiology 2022Quote: ... (ii) the 3’ adapter was ligated to protein/RNA complexes on antibody-conjugated Dynabeads (ThermoFisher 10004D) instead of ligating to purified RNAs ...
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control ...
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bioRxiv - Cell Biology 2022Quote: Human proteins (LIS1-SNAP, or motor domains) were expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described with minor modifications3,7,20,69 ...
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bioRxiv - Immunology 2022Quote: ... The gB Domain I and Domain I+II were purified using Nickel-NTA resin (Thermo Fisher Scientific), and the gB Domain II was purified with lectin resin (VWR.)
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bioRxiv - Genomics 2023Quote: ... Samples were placed into separate PCR tubes containing 3 μl of lysis buffer with 0.3 mg/ml protein kinase K (Thermofisher, 26160). Subsequently ...
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bioRxiv - Genetics 2022Quote: ... and cloned into the pDEST32 vector containing the GAL4 DNA binding domain (DBD) through Gateway cloning (ThermoFisher, 11789020 and 12538120) and used as the Y2H bait construct ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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A sound strategy for homology modeling-based affinity maturation of a HIF-1α single-domain intrabodybioRxiv - Biochemistry 2020Quote: The HIF-1α-PAS-B domain was coated at 3 μg/ml onto 96-well microtiter plates (Thermo Fisher Scientific) and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Cys469 in the C-terminal DNase domain of these ColE9 constructs was labelled with a 3-fold Alexa Fluor 488-maleimide (Invitrogen) as previously described (41) ...
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bioRxiv - Neuroscience 2019Quote: ... NIM was exchanged for “3:1 medium” containing 3 parts DMEM (Gibco, #10569‒010) per 1 part F12 medium (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... and 40 µg of antibody was bound to 3 mg of protein G Dynabeads (Thermo Fisher Scientific) in 400 µL of PBS buffer containing 0.2% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Soluble material was incubated with 3 μg of antibody bound to 50 μl protein A Dynabeads (Invitrogen) and incubated overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: DNA constructs encoding anti-SCARB2 antibody heavy and light chain variable domains (clone JL278) were synthesized (ThermoFisher) and cloned into mammalian expression vectors containing a mouse IGHV signal peptide and IgG1 constant regions ...
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bioRxiv - Genetics 2020Quote: ... Nuclear extracts containing the NFIA protein were extracted from 3 wells of transfected cells using nuclear and cytoplasmic extraction kit (Thermo Scientific). 1 mM ssODNs (PIK3C2B ...
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bioRxiv - Genetics 2022Quote: ... elegans genes was cloned into the pDEST22 vector containing the GAL4 activating domain (AD) using the CloneMinerTM cDNA library construction kit (ThermoFisher, A11180). A forward Y2H library screen was performed using the ProQuest Two-Hybrid System (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... This oligo was annealed and amplified to a fixed 91nt oligo containing the scaffold domain using Taq polymerase (Invitrogen, Cat. #10342046). Transcription was performed using Hi-SCribe T7 ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed 3 times with PBS containing 0.5% BSA and stained with an AF647 conjugated donkey anti-mouse IgG antibody (Invitrogen) for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were washed 3 times with PBS containing 0.5% BSA and stained with an AF647 conjugated donkey anti-mouse IgG antibody (Invitrogen) for 1 h ...
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bioRxiv - Genomics 2023Quote: ... Chromosomes were stained with anti-Elys antibodies [65] (1:400) diluted in PBT containing 0.3% Triton X-100 and 3% Normal Goat Serum (Invitrogen) and counterstained with Dapi ...
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bioRxiv - Neuroscience 2021Quote: ... PBS containing 1 µM TO-PRO 3 (Thermo Scientific) was applied to each section followed by 3 washes with PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 1 M sodium chloride (S671-3, Fisher Scientific). The labelling protocol was carried out at pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.75) containing 3 µM Avidin (Thermo Fisher # A887) 200 µM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Biochemistry 2023Quote: Expression and purification of secreted SIDT1 and SIDT2 extracellular domain The SIDT1ECD and SIDT2ECD proteins were expressed using FreeStyle 293-F cells (Invitrogen). The cells were cultured in OPM-293 CD05 medium (OPM Biosciences co. ...