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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Spleen Focus Forming Virus Proviral Integration Oncogene SPI1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Infectious titers were determined by focus-forming unit assay whereby foci of infection were measured using anti-nucleocapsid (N) antibody (Invitrogen; MA1-7403). Where indicated ...
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bioRxiv - Microbiology 2024Quote: ... Viral titers were determined by counting the number of focus-forming units (FFU) containing HEV ORF2-positive cells through image analysis using the HCS Studio Cellomics software (Thermo Fisher Scientific, Version 6.6.2).
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bioRxiv - Cell Biology 2023Quote: ... HT914 and HT915 iPSC lines) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) as previously reported [43–45] ...
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bioRxiv - Cancer Biology 2021Quote: Heathy human donor PBMCs or skin fibroblasts were reprogrammed into iPSCs using the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Low passage primary fibroblasts were used to increase reprogramming efficiency ...
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bioRxiv - Cell Biology 2019Quote: Fibroblasts isolated either from human fetal liver tissues or human adult skin after collagenase dissociation were reprogrammed into iPSCs with the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Fibroblasts were used at low population doubling (less than 5 ...
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bioRxiv - Immunology 2023Quote: WT THP1 cells were transfected with 60 pmol of SPI1 targeting (ThermoFisher cat# HSS186060) or Cy3-conjugated non-targeting siRNA (ThermoFisher cat# AM4621 ...
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bioRxiv - Developmental Biology 2019Quote: Peripheral blood CD34+CD38- cells mobilized from healthy donors were reprogrammed into iPSCs (MCND-TENS2 line) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) following the method previously reported30,31 ...
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bioRxiv - Neuroscience 2023Quote: ... and CD4-positive spleen cells from the spleen with Dynabeads magnetic beads-conjugated CD4 antibody (clone L3T4; Thermo Fisher Scientific). Lysis buffer was immediately added to selected cells and total RNA was isolated using RNeasy Plus Mini Kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: 293T cells were transfected with HIV-1 proviral DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Immunology 2022Quote: ... Proviral loads were measured by the QuantStudio™ 3D Digital PCR System (Life Technologies). 300-700 ng of cellular DNA (per chip ...
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bioRxiv - Cancer Biology 2020Quote: ... T-HEp3 with the biosensors were reversely transfected with siRNAs (siTGFBRIII, Invitrogen 32274204; siBMPRII, Oncogene SR300456B) using Lipofectamine RNAiMAX transfection reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... stable integration was confirmed by Hygromycin (Invitrogen) selection at a final concentration of 200 μg/mL ...
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bioRxiv - Pathology 2023Quote: ... Q Exactive Focus system (Thermo Fisher Scientific) was used to verify the identity of each peak observed in HPLC fractions ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used: anti-Influenza A virus NS1 (PA5-32243, ThermoFisher), anti-acetyl-α-tubulin (Ly640 ...
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bioRxiv - Molecular Biology 2022Quote: ... Transgene integration selection was performed with puromycin (Gibco) before single cell clones were generated by limited dilution ...
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bioRxiv - Immunology 2023Quote: ... and then spleen samples were incubated with CD16/CD32 antibody (Invitrogen #14-0161-86) to block Fc receptors ...
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bioRxiv - Microbiology 2020Quote: The HEK293T and HeLa cell lines were transfected with HIV-1 proviral DNA using Lipofectamine 2000 (Invitrogen). At 48 hrs post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Colony-forming attached cells were passed by trypsin (12605-010, Invitrogen, USA) for further experimental use.
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bioRxiv - Cell Biology 2023Quote: ... All integrations were confirmed by PCR (Thermo Fisher Scientific). Gene deletions were confirmed by PCR and Southern blotting.
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bioRxiv - Biochemistry 2024Quote: ... Peak integration was performed using Tracefinder 5.1 (Thermo Scientific).
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bioRxiv - Microbiology 2019Quote: ... Other antibodies were obtained from commercial vendors as follows: Anti-coxsackie virus B3 antibodies (ThermoFisher), Anti-adenovirus A5 antibodies (ThermoFisher) ...
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bioRxiv - Cell Biology 2021Quote: ... Colony-forming attached cells were passed twice by trypsin (12605-010, Invitrogen, USA) for further experimental use.
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bioRxiv - Microbiology 2021Quote: ... Absolute numbers of copies of proviral DNA were determined by real time PCR using custom made probes (Thermo Fisher). To obtain absolute proviral DNA copy numbers ...
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bioRxiv - Microbiology 2024Quote: ... and the HIV-1 latently infected CD4+ CEM cells ACH-2 (A3.01 cell integrated HIV-1 proviral DNA) were cultured in complete RPIM640 (Gibco) containing 10% FBS and 1% penicillin/streptomycin at 37°C in an atmosphere of 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were selected for plasmid integration with geneticin (G418, ThermoFisher). MDCK Claudin-quintuple knockout (Cldn-qKO ...
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bioRxiv - Developmental Biology 2019Quote: ... and screened for reporter integration by PCR (Thermo Fisher, EP1701) using the primers listed in Table S4 ...
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bioRxiv - Cell Biology 2021Quote: N-terminally MBP-tagged (pTHMT) FUS CHOP fusion type II and CHOP oncogene were expressed in Escherichia coli BL21 Star (DE3) cells (Life Technologies). Bacterial cultures were grown to an optical density of 0.7–0.9 before induction with 1 mM isopropyl-b-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Genetics 2023Quote: ... near full-length HIV proviral amplification was performed on genomic DNA extracted from CD4+ T-cells using Platinum Taq DNA Polymerase High Fidelity (Invitrogen), where IPDA-determined total proviral loads were used to dilute DNA such that no more than 30% of resulting nested PCR reactions yielded an amplicon (protocol described in 40) ...
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bioRxiv - Microbiology 2023Quote: ... infected cells were stained with primary antibody (Vaccinia Virus Polyclonal FITC Antibody ThermoFisher PA1-73191, 1:1000) and Hoechst 33342 (1:10,000) ...
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bioRxiv - Microbiology 2019Quote: ... Images were recorded in focus with a Volta phaseplate (ThermoFisher) for WT and without phaseplate at 5 μm under-focus for ΔJF50_12610 ...
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bioRxiv - Developmental Biology 2021Quote: ... The Q Exactive Focus Orbitrap Mass Spectrometer (Thermo Fisher Scientific) equipped with HESI sources was used for metabolite identification ...
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bioRxiv - Cell Biology 2023Quote: ... An orbitrap MS system (Q-Exactive focus, Thermo Fisher Scientific) connected to a high-performance IC system (ICS-5000+ ...
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bioRxiv - Cancer Biology 2020Quote: ... UPS primary tumors and spleens and naïve KP spleens were stained for regulatory T cells using anti-FoxP3 APC (clone FJK-16s, Invitrogen) and the FoxP3/transcription factor staining buffer set (eBioscience ...
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bioRxiv - Systems Biology 2019Quote: ... Acquisition and integrations were done with Xcalibur™software (ThermoFisher Scientific).
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bioRxiv - Immunology 2024Quote: ... the cells forming the pellet were reconstituted in red blood cell lysis buffer (Thermo Fisher) and subjected to a 2-minute incubation on ice to lyse the red blood cells ...
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bioRxiv - Genomics 2022Quote: ... Spleen dissociation buffer was PBS (pH 7.4, Gibco). Spheres were sorted into 30 μl of PBS and dissociated by applying mechanical force pipetting up and down around 50 times using a P20 multichannel pipette ...
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bioRxiv - Bioengineering 2023Quote: Human spleen total RNA (Thermo Fisher Scientific, USA) was used as template for cDNA synthesis using Superscript IV 1st strand (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... namely a Thermo Q-Exactive Focus instrument (Thermo Fisher Scientific, USA) equipped with a Dionex Unimate 3000 UHPLC system was used ...
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bioRxiv - Cancer Biology 2023Quote: For tumorsphere forming assays cells were cultured in DMEM-F12 1:1 (Gibco, Waltham, Massachusetts, USA) containing B27 (1:50) ...
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bioRxiv - Neuroscience 2023Quote: ... Relative amounts of proviral DNA have been determined by real time PCR using a StepOnePlus Real-Time PCR System (Life Technologies Limited). PCR reactions were carried out in 96 well plates following a standard thermal cycling protocol with an overall reaction volume of 20µl ...
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bioRxiv - Cell Biology 2020Quote: ... For peak integration the software TraceFinder™ (version 4.1 - Thermo Scientific, Austria) was used.
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bioRxiv - Molecular Biology 2023Quote: ... and quantified by peak area integration using the TraceFinder 4.1 (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... 0.5% BSA in PBS pH7.2) and stained using appropriate antibody mixes or single stain controls (spleen or OneComp eBeadsTM from ThermoFisher). After staining ...
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bioRxiv - Microbiology 2020Quote: ... p53 was amplified from spleen cDNA library (Invitrogen, USA). For MV-C was amplified from pTM-Schwarz using different primers ...
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bioRxiv - Microbiology 2019Quote: Spleen cells were released into RPMI 1640 media (Gibco) by mincing the spleen through a 70 μm cell strainer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Spleens were placed in RPMI-1640 medium (Gibco, Invitrogen) supplemented with 5% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... spleens and lymph nodes was isolated using TRIzol (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Spleens were placed in RPMI-1640 medium (Gibco, Invitrogen) supplemented with 5% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... spleens and lymph nodes was isolated using TRIzol (Invitrogen) following the manufacturer’s instructions ...