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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for SEAP ASSAY KIT since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... SEAP activity was measured using Phospha-lightTM assay system (Thermofisher) according to manufacturer’s instructions with Hidex Sense Microplate Reader (Labgene).
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bioRxiv - Molecular Biology 2023Quote: ... The transfection efficiency was controlled by quantification of the SEAP using the Phospha-Light™ SEAP Reporter Gene Assay System (T1015, ThermoFisher) and a 1:20 dilution of the supernatant ...
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bioRxiv - Microbiology 2020Quote: ... SEAP concentration was tested on 50µl media supernatants of the samples using CPSD (Tropix PhosphaLite Chemiluminescent Assay Kit, Life Technologies cat no. T1017) using a minor variant of the manufacturer’s instructions and luminescence intensity was measured using a Varioskan Flash luminometer (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2019Quote: ... SEAP activity was measured 24 hours after transfection using the Phospha-Light kit (Thermo Scientific) and POLARstar OPTIMA microplate reader (BMG Labtech) ...
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bioRxiv - Synthetic Biology 2023Quote: ... cell culture medium was added to SEAP sample buffer and SEAP substrate in a transparent 96 well plate (Thermo Fisher Scientific), according to manufacturer’s instructions (in a final concentration of 0.83 mg/mL pNPP) ...
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bioRxiv - Synthetic Biology 2023Quote: SEAP levels were determined by adding QUANTI-Blue reagent (Invitrogen), whose change in color intensity from pink to purple/blue is proportional to the enzyme’s activity ...
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bioRxiv - Physiology 2020Quote: ... phenyl dihydrogen phosphate (CSPD) chemiluminescent substrate and the Phospha-Light SEAP Reporter Gene Assay System according to the manufacturer’s protocol (Thermo Fisher Scientific, Waltham, MA). Luminescence levels were detected using a Synergy 2 microplate reader set for an endpoint read with a 2s integration time ...
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bioRxiv - Cell Biology 2021Quote: ... Conditioned media were then collected and analyzed for secreted alkaline phosphatase activity (SEAP) using a commercial kit (T1015, Invitrogen) and a microplate reader47–49.
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bioRxiv - Immunology 2020Quote: ... SEAP expression was measured 24 hours later with the chemiluminescent substrate from the Phospha-Light™ kit (Applied Biosystems). Neutralization titers were defined as the dilution at which a 50% reduction of SEAP activity from serum sample was observed relative to SEAP activity from virus alone.
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bioRxiv - Immunology 2021Quote: ... and transfected into SEAP HEK cells using TurboFect transfection reagent (Thermo Fisher Scientific, UK) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then mixed with 100 μL of 2X SEAP buffer (20 mM homoarginine (ThermoFisher catalog# H27387), 1 mM MgCl2 ...
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bioRxiv - Microbiology 2024Quote: ... SeAP was measured in 25 uL of media per infection using the Phospha-Light System (Applied Biosystems). SeAP values from media alone were subtracted from each experimental value ...
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bioRxiv - Neuroscience 2022Quote: ... BCA protein assay kit (ThermoFisher Pierce BCA Protein Assay) was used to determine the protein concentration ...
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bioRxiv - Molecular Biology 2019Quote: ... Optionally peptide quantification assays (colorimetric assay kit, Thermo Scientific) were carried out using the Bravo liquid handling system.
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bioRxiv - Genomics 2022Quote: Qubit RNA Assay Kit (ThermoFisher)
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bioRxiv - Plant Biology 2021Quote: ... Assay Kit (Invitrogen, Paisley, UK).
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bioRxiv - Cell Biology 2019Quote: ... Qubit protein assay kit (Invitrogen) was used to obtain protein concentration ...
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bioRxiv - Plant Biology 2021Quote: ... Assay Kit (Invitrogen, Paisley, UK).
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bioRxiv - Bioengineering 2023Quote: ... PresoBlue assay kit (P50200, ThermoFisher) was utilized by following the standard protocol ...
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bioRxiv - Cell Biology 2021Quote: CHO-K1 cells (ATCC: CCL-61) and CHO-SEAP cells [61] were cultured in F-12K medium (Gibco), or Iscove’s Modified Dulbecco’s Medium (IMDM) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected with the calcium dependent-SEAP construct (248 ng·well−1) using a standard Lipofectamine 2000 (2.5 ng:1 ng DNA; Invitrogen) protocol and supplemented with 2 mg·mL−1 holo-transferrin (Thermo 11107018) ...
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bioRxiv - Cell Biology 2020Quote: LDH assay (Pierce LDH Cytotoxicity Assay Kit; 88954, ThermoFisher Scientific) was performed following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... BCA assay (Pierce BCA protein assay kit, 23225, Thermo Scientific) was performed to determine protein concentrations ...
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bioRxiv - Bioengineering 2020Quote: ... The lactate dehydrogenase assay (LDH Assay Kit®, Thermo Scientific) and Presto Blue® assay (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... BCA assay (Pierce BCA protein assay kit, 23225, Thermo Scientific) was performed to determine protein concentration ...
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bioRxiv - Biophysics 2023Quote: ... Assays were performed using the EnzChek Phosphate Assay Kit (Invitrogen) modified for a 96 well plate format 49 ...
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bioRxiv - Bioengineering 2023Quote: DNA assay kit QuantiT dsDNA HS kit (Invitrogen) was used to quantify the DNA content from cell lysate according to the manufacturers protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... This mixture was then mixed with with 100 uL of 2X SEAP Buffer (20 mM homoarginine (ThermoFisher catalog# H27387), 1 mM MgCl2 ...
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bioRxiv - Biochemistry 2019Quote: The microscale BCA assay (Micro BCA Protein assay kit (Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2019Quote: ... A Pierce™ Bicinchoninic Acid assay (BCA) Protein Assay Kit (ThermoFisher) was used to quantify the isolated LDL/VLDL (Apolipoprotein B protein expression) ...
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bioRxiv - Cell Biology 2022Quote: ATPase assays were performed using the EnzChek Phosphate Assay Kit (Invitrogen). 50 μL reactions contained 200 nM recombinant condensin II in reaction buffer (40 mM Tris [pH 7.5] 15 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ATPase assays were performed using the EnzChek phosphate assay kit (Invitrogen) with motor concentrations in the 5 to 50 nM range and 25nM to 2 µM polymerized tubulin ...
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bioRxiv - Developmental Biology 2022Quote: ... A bicinchoninic acid assay (Pierce BCA Protein Assay Kit, ThermoFisher Scientific) was used to quantify the protein extract supernatant according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: GTPase assays were performed using the Enzchek phosphate assay kit (Invitrogen). Reactions were performed in a 100-μl volume with 5 μl 20× reaction buffer (1 M Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: TUNEL assay performed using Click-IT Plus TUNEL assay kit (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... following kit instructions (BCA Protein Assay kit, ThermoFisher #23225) and 20µg loaded into each well of a NuPAGE 4-12% Bis-Tris Gel (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Assay Kit (Catalog: Q32854, ThermoFisher Scientific). The Qubit™1X dsDNA HS Assay Kit ...
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bioRxiv - Molecular Biology 2020Quote: ... Vybrant™ Phagocytosis Assay Kit (Invitrogen) was utilized to evaluate phagocytosis alterations in HMC3 cells with and without Aβ42 treatment and TREM2 overexpression or silencing ...
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bioRxiv - Cancer Biology 2019Quote: ... Vybrant apoptosis assay kit (Thermofisher, V13243) was used to stain cells simultaneously with PI and YO-PRO1 as per manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... using custom ProcartaPlex assay kits (Invitrogen). Analyte concentrations were determined using a Luminex100/200 Flexmap3D instrument coupled with Luminex XPONENT software (Luminex ...
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bioRxiv - Microbiology 2020Quote: ... Qubit HS DNA Assay Kit (Invitrogen), and 1% agarose gel migration ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using HS Assay Kit (Invitrogen, Q32851). A subset of samples was quality controlled to check the RIN values by Bioanalyzer using the RNA 6000 Pico Kit (Agilent ...
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bioRxiv - Molecular Biology 2021Quote: ... and Qubit dsDNA Assay kit (Invitrogen). The membrane peroxidation index (PI ...
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bioRxiv - Neuroscience 2022Quote: ... BCA Protein Assay kits (Thermo Fisher) were used to determine protein concentrations ...
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bioRxiv - Genomics 2022Quote: Qubit dsDNA HS Assay kit (ThermoFisher)
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bioRxiv - Microbiology 2019Quote: ... cytokine Assay kit (Thermo Scientific, USA), DAF-2 DA ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with dsDNA HS Assay Kit (Invitrogen), and checked the product size by 4200 Tapestation (Agilent ...
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bioRxiv - Developmental Biology 2020Quote: ... Qubit dsDNA Assay kit (Life Technologies) and TapeStation 4200 (Agilent ...
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bioRxiv - Immunology 2021Quote: The PrestoBlue assay kit (Thermo Fisher) was used to measure fungal viability following co-culture with WT and Skap2-/- DIV neutrophils (Tam et al. ...
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bioRxiv - Immunology 2022Quote: ... Quant-it protein assay kit (ThermoFisher), and Syproruby protein gel stain (ThermoFisher).