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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... coli and BacuroDirect C-term Linear DNA (Thermo Fisher Scientific, #12562019) for protein expression in insect cells by the Gateway LR reaction ...
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bioRxiv - Microbiology 2022Quote: ... and stained using SARS/SARS-CoV-2 Coronavirus Nucleocapsid Monoclonal Antibody (Invitrogen MA5-29981) as the primary antibody followed by Alexa647-conjugated secondary antibody (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... antibodies targeting the SARS-CoV-2 nucleoprotein (N) (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... For the SARS-CoV-2 staining a polyclonal rabbit anti-SARS Coronavirus Spike protein (PA1-41142, Thermo Fisher, France) and revealed using an anti-rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...
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bioRxiv - Biophysics 2022Quote: ... Escherichia coli (E. coli) BL21-AI cells (Invitrogen) were transformed with a modified pET vector encoding N-terminal 10X His tagged VcINDY ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were maintained in short-term monolayer cultures in William’s E (Gibco) supplemented with 1% Glutamine (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were incubated with rabbit anti-SARS coronavirus spike protein polyclonal antibody (Invitrogen, PA1-41513) diluted 1:10,000 ...
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bioRxiv - Cell Biology 2022Quote: ... to 1 µg/ml and mouse SARS-CoV-2 Nucleoprotein monoclonal antibody (MA1-7404, Thermofisher) to 10 µg/ml ...
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bioRxiv - Bioengineering 2019Quote: ... fluorescent Escherichia coli (E. coli) BioParticles (ThermoFisher, cat. #E2863), or fluorescent carboxylate-modified 1 µm polystyrene beads (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... incubated with SARS-CoV-2 Nucleoprotein polyclonal antibody at 1:500 dilution (Invitrogen, Carlsbad, CA, USA) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: Glycerol stock of Escherichia coli (E. coli) (TOP10 strain, Invitrogen, Thermo Fisher Science ...
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bioRxiv - Immunology 2020Quote: ... Anti-SARS-CoV-2 Coronavirus Spike protein (subunit 1) polyclonal antibody was purchased from Invitrogen (Carlsbad, CA, USA). Anti-SARS-CoV-2 Spike RBD rabbit polyclonal antibody was purchased from SinoBiological (Beijing ...
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bioRxiv - Bioengineering 2021Quote: ... The anti-His(C-term)-HRP antibody (R931-25, Invitrogen) was used ...
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bioRxiv - Evolutionary Biology 2023Quote: We individually subcloned HRR25 orthologs to pKB11 (pDEST-DHFR F[1,2] C-term NatR) and pDN0605 (pDEST-DHFR F[1,2] N-term NatR) by LR cloning (Invitrogen, 11791), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 E protein was purchased from ThermoFisher Scientific (Catalog# RP-87682) ...
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bioRxiv - Microbiology 2020Quote: Human IFITM proteins and SARS-CoV-2 viral proteins were cloned into MaMTH N-term tagged Prey and C-term tagged Bait vectors respectively using Gateway cloning technology (ThermoFisher). Correctness of recombined insertions was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Molecular Biology 2024Quote: ... coli poly(A) polymerase (E-PAP, Invitrogen) at 37°C for 30 min in 50 µL reactions ...
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bioRxiv - Microbiology 2024Quote: ... To transform Stbl3 E coli cells (Invitrogen) with the BAC construct ...
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bioRxiv - Microbiology 2019Quote: ... USA. Escherichia coli DH5α (hereafter called E. coli) was purchased from Invitrogen Inc. ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli strain DH5α (E. coli DH5α, Thermo Fisher Scientific, Cat#18258012), E ...
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bioRxiv - Microbiology 2021Quote: ... or SARS-CoV-2 E protein (1-75 aa) (Thermo Fisher) in coating buffer [0.05 M sodium carbonate–sodium bicarbonate (pH 9.6)] ...
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bioRxiv - Microbiology 2022Quote: ... of the SARS coronavirus strain Tor2 (NC_004718.3) was ordered as a synthetic DNA string fragment (GeneArt synthesis; Thermo Fisher Scientific, Darmstadt, Germany) and cloned into the pEXPR103 expression vector (IBA Lifesciences ...
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bioRxiv - Microbiology 2023Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... kanamycin (Fisher Scientific; 40 µg/mL for E. coli), gentamicin (Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-His (C-term)-HRP (#46-0707, 1:2000 for dot blot, Invitrogen); Aβ (6E10 ...
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bioRxiv - Microbiology 2022Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Microbiology 2023Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were then blocked with 5% dried milk in PBS-0.1%Tween followed by incubation with SARS Coronavirus NP Monoclonal Antibody (E16C) (ThermoFisher Scientific, Catalog # MA1-7403) or SARS Coronavirus Spike polyclonal Serum (BEI Resources ...
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bioRxiv - Biophysics 2020Quote: CLGltPh and double cysteine mutants were transformed into Escherichia coli (E. coli) Top10 cells (Invitrogen). Bacterial cells were inoculated in Luria Broth medium containing 100 μg/mL ampicillin at 37 °C until OD600 ~0.6-0.8 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Coronavirus pan monoclonal antibody (FIPV3-70, Invitrogen) was combined with a secondary antibody using Alexa Fluor 647 fluorophore (10739374 ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.09 μL Ambion RNase H (E. coli) (Invitrogen, AM2293). Second strand synthesis was performed by incubating samples at 16°C for 150 min ...
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bioRxiv - Genomics 2019Quote: ... 1 µl of 2 U/µl E Coli RNaseH (Invitrogen) and then incubating at 16°C for 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: Escherichia coli (E. coli) strain DH5α (Toyobo, Osaka, Japan) and BL21(de3) pLysS (C606003, ThermoFisher Scientific) were used for DNA cloning and protein expression ...
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bioRxiv - Genomics 2022Quote: ... vector and amplified in DH5α strain of Escherichia coli (E coli) (Life Technologies, Grand Island NY). After amplification ...
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bioRxiv - Developmental Biology 2021Quote: Escherichia coli (E. coli) strain DH5α (Toyobo, Osaka, Japan) and BL21(de3) pLysS (C606003, ThermoFisher Scientific) were used for DNA cloning and protein expression ...
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bioRxiv - Microbiology 2024Quote: Glycerol stock of Escherichia coli (E. coli) (TOP10 strain, Invitrogen, Thermo Fisher Science, Waltham, MA, USA) transformed with plasmid pCR2.1 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 E protein (1 to 75 amino acids) (ThermoFisher Scientific) in coating buffer [0.05 M sodium carbonate-sodium bicarbonate (pH 9.6)] at 4°C for overnight incubation ...
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bioRxiv - Microbiology 2022Quote: ... The presence of recombinant virus was verified by immunostaining with a monoclonal antibody against the RABV nucleoprotein (FujiRebio, Cat# 800–092) and polyclonal antiserum against the SARS CoV/CoV-2 S1 domain (Thermo Fisher, Cat# PA581798). The filtered virus was then used to inoculate Vero cells seeded in Cellstack Culture Chambers (Corning ...
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bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
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bioRxiv - Biochemistry 2020Quote: ... were expressed in the bacterial Escherichia coli (E. coli) BL21(DE3) pLysS strain (Invitrogen, Thermo Fisher Scientific) as described in 38 ...
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bioRxiv - Cell Biology 2023Quote: ... strain was maintained on standard nematode growth media (NGM) plates seeded with OP50 E. coli bacteria (at 20°C. LysoSensor Green DND-189 (Thermo Fisher) was diluted in M9 buffer and added to 6 cm standard NGM plates (containing 12 mL of agar ...
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bioRxiv - Immunology 2020Quote: ... 20⧅l of opsonised Escherichia coli (E.coli) was added to the tube (pHrodo Red E. coli BioParticles; ThermoFisher). The bacteria was initially prepared by mixing the E.coli strain with new-born calf sera followed by a 30-minute incubation at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... All the SAM with natural isotope abundance and 2H or 13C enrichment were enzymatically synthesized from L-methionine and ATP with appropriate isotope labels. Escherichia coli (E. coli) DH5α and BL21(DE3) competent cells were from Invitrogen. Data plotting and nonlinear least-squares fitting was carried out using GraphPad Prism 7 software ...
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bioRxiv - Biochemistry 2021Quote: ... and TnsB (WP_084763316.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...
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bioRxiv - Immunology 2019Quote: ... or pH sensitive fluorescent bioparticles (pHrodo E. coli Bioparticles Conjugate for Phagocytosis; Invitrogen). Each fly was injected with 27.6-32.2 nl of bioparticles (1mg/100µl ...
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bioRxiv - Biochemistry 2019Quote: ... Filtrate was added onto DNA Polymerase 1 (PolI) (E. coli source, Fisher Scientific), 0.25U/uL reaction ...
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bioRxiv - Biophysics 2024Quote: ... Ligated vectors were transformed into competent bacteria (One Shot TOP10 E. coli; Invitrogen) and grown overnight on ampicillin plates ...
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bioRxiv - Cell Biology 2022Quote: ... DNA polymerases and NEBuilder HiFi DNA assembly master mix were obtained from New England Biolabs. Cloning was performed in TOP10 chemically competent Escherichia coli (E. coli) (ThermoFisher Scientific). Buffers for assessing fluorescence lifetime were composed of either 0.01M PBS (pH 6.5 – 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... and Escherichia coli S15 (AP009048.1) proteins were codon optimized for heterologous expression in Escherichia coli (E. coli) and synthesized by GeneArt (Thermo Fisher Scientific). The ShCas12k gene was inserted into the 1B (Addgene 29653 ...