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Citations for Thermo Fisher :
1 - 50 of 3592 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
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bioRxiv - Immunology 2021Quote: ... spike glycoproteins were produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Immunology 2021Quote: ... A CMV promoter/enhancer with an SV40 polyA was introduced into the E1 region and the spike gBlock sequences introduced by Gibson assembly (Codexis) and transformed into Stbl4 (Thermo Fisher) cells ...
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bioRxiv - Bioengineering 2021Quote: Recombinant coronavirus spike glycoproteins S (Table S1) were coated overnight onto NUNC® Immulon 4 HBX microtiter plates (Thermo Fisher) at 50 ng/well in 100 µL of PBS ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding spike variants were transfected into FreeStyle 293-F cells (Thermo Fisher) in 3 mL of medium and harvested four days after transfection ...
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bioRxiv - Cancer Biology 2022Quote: Glycoproteins were isolated using a glycoprotein isolation kit (89804; Thermo Fisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Glycoproteins were produced by transient transfection of exponentially growing Freestyle 293-F suspension cells (Thermo Fisher Scientific, Waltham, MA) using polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2022Quote: ... Glycoproteins were produced by transient transfection of exponentially growing Freestyle 293-F suspension cells (Thermo Fisher Scientific, Waltham, MA) using polyethylenimine (PEI ...
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bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
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bioRxiv - Biochemistry 2022Quote: ... was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... glycoproteins were digested with trypsin (ThermoFisher) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and Pierce Glycoprotein Staining kit (ThermoFisher) were used for protein and glycan staining ...
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bioRxiv - Biochemistry 2021Quote: ... while the other was stained for glycoproteins using the Pro-Q Emerald 300 glycoprotein staining kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies used: cyclin E1 (Invitrogen, cat# 32-1500) & cyclin E (Cell Signaling Technologies ...
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bioRxiv - Genetics 2022Quote: ... Tissues were incubated with mouse anti-P-glycoprotein C219 antibody at 3.6μg/mL (Glycoprotein Monoclonal Antibody C219, Cat. No MA1-26528, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A glycoprotein ladder (Candy Cane ladder; Invitrogen) served as positive control ...
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bioRxiv - Microbiology 2024Quote: ... along with a CandyCane glycoprotein ladder (Thermofisher). We additionally loaded the same two B ...
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bioRxiv - Cancer Biology 2020Quote: ... cyclin E1 (Hs00180319_m1) and human RPLPO (4326314E; Applied Biosystems). Analysis was performed as previously described using the ΔΔCT method (25).
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bioRxiv - Systems Biology 2020Quote: ... and a second part was stained and analysed for glycoproteins using the Pro-Q 488 Emerald Glycoprotein staining kit (P33375, Thermo/Invitrogen), according to the manufacturers protocol ...
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bioRxiv - Immunology 2023Quote: ... The full-length spike proteins were stabilized by introducing 2 prolines at amino acid positions 986 and 987 (Spike 2P) and transiently transfected in FreeStyle 293-F cells using Turbo293 (SpeedBiosystems) or 293Fectin (ThermoFisher). The constructs contained an HRV3C-cleavage site followed by His and/or streptagII tags for purification purposes ...
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bioRxiv - Immunology 2022Quote: ... and transfected into E1-complement HEK293A cells (ThermoFisher, R705-07) for the adenovirus packaging and generation ...
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bioRxiv - Immunology 2020Quote: ... spike protein was expressed by transiently transfecting plasmid encoding the HexaPro spike variant2 containing substitutions S383C and D985C3 with a C-terminal TwinStrep tag into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Molecular Biology 2020Quote: Escherichia coli strain BL21-AI [F-ompT hsdSB (rB-mB-) gal dcm araB∷ T7RNAP-tetA] (Thermo Fisher Scientific) was used in the plasmid interference assay ...
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bioRxiv - Developmental Biology 2022Quote: ... or with Pierce™ Glycoprotein Staining Kit (Thermo Scientific) after separation.
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bioRxiv - Molecular Biology 2019Quote: ... 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific, E304050), 2.4 µM human His6-UBE2T E2 enzyme (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The ectodomain of ancestral SARS-CoV-2 spike (GenBank: MN908947.3) was expressed as previously described.10 The protein was purified from FreeStyle 293-F cells (Thermo Fisher Scientific, USA) using affinity chromatography followed by size exclusion chromatography ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain BL21 Star™ (DE3) (genotype F-ompT hsdSB (rB-, mB-) gal dcm rne131 (DE3)) (Invitrogen, Waltham, MA) was used for the cell-extract for the cell-free protein synthesis reaction ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Pro-Q™ Emerald 300 glycoprotein gel stain kit (Invitrogen) was used to detect the presence of sugar in Bl-Eng2 before and after deglycosylation ...
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bioRxiv - Developmental Biology 2019Quote: ... ERCC spike-in (Invitrogen) were added in the lysis buffer (1:1000000 ...
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bioRxiv - Cancer Biology 2024Quote: ... ERCC spike-in (Invitrogen) was added at a ratio of 2uL of spike-in per 1ug of RNA.
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bioRxiv - Biochemistry 2020Quote: ... coli strain (ATCC 11303 strain, 14380, Affymetrix) were used.
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bioRxiv - Cell Biology 2019Quote: ... MC3T3-E1 (ATCC) were maintained in MEM Alpha without ascorbic acid (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... ERCC Spike-In Controls (Invitrogen) were added to RNA samples (input RNA was 100ng per sample ...
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bioRxiv - Cancer Biology 2020Quote: ... ERCC Spike-In Mix (ThermoFisher) was used as an internal control ...
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bioRxiv - Plant Biology 2020Quote: ... ArrayControl RNA Spikes (Applied Biosystems; www.thermofisher.com/applied/biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... ERCC spike-in controls (ThermoFisher) were added to 500 ng of total RNA and ribominus selection was performed using the NEBNext® rRNA Depletion Kit ...
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bioRxiv - Neuroscience 2022Quote: ... ERCC Spike-In Controls (Invitrogen) were added to 100ng of RNA per sample prior to processing with the NEBNext Poly(A ...
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bioRxiv - Cell Biology 2020Quote: ... ERCC spike-in RNA (Ambion) was added to the lysis buffer in a dilution of 1:4,000,000 ...
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bioRxiv - Immunology 2022Quote: ... desalted (Zeba Spike Desalting, ThermoFisher), and loaded at a concentration of 500 nM onto streptavidin (SA ...
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bioRxiv - Microbiology 2022Quote: ... ERCC spike mix 2 (Ambion) was added to the RNA ...
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bioRxiv - Microbiology 2023Quote: ... ERCC spike-in controls (ThermoFisher) were added to 500-1000 ng of total RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA spike-ins (Invitrogen, 4456740) were added to each purified RNA sample following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... ERCC spikes (Thermo Fisher Scientific) were added to samples to control all the procedure ...
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bioRxiv - Genomics 2019Quote: ... An ArrayControl RNA Spots and Spikes kit (with spike numbers 1, 4 and 7) (Ambion) were used to monitor technical variability ...
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bioRxiv - Microbiology 2023Quote: ... coli strains DH5α and DH10B strains (both from Invitrogen) were used for molecular cloning ...
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bioRxiv - Immunology 2021Quote: ... and Spike-Alexa488 (Thermofisher Scientific, S32354) and Spike-APC (Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and Spike-APC (Thermofisher Scientific, S32362) or RBD-Alexa488 and RBD-APC ...
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bioRxiv - Developmental Biology 2021Quote: ... and ERCC spike-in RNA (Ambion) (1:1,000,000 dilution ...
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bioRxiv - Plant Biology 2021Quote: ... root and spike using Trizol (Invitrogen) method and Xcelgen plant RNA kit ...