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Citations for Thermo Fisher :
1 - 50 of 4782 citations for Rpb1 CTD Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: The biotinylated CTD peptides were synthetized by Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and immediate collection of bead bound RNA and transcription complexes in Trizol following immunoprecipitation by Rpb1 CTD (4H8) mouse mAB conjugated to Dynabeads M-280 sheep anti-mouse IgG (ThermoFisher). mNET-seq experiments were completed in biological replicate ...
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bioRxiv - Genomics 2019Quote: ... Starting with a mixture of DNA of seven BACs (CTC-775N13, RP11-715G8, CTD-3048C22, CTD3055E11, CTD-2643I7, CTD-3234J1, and RP11-589G14) (Invitrogen), mixed in equimolar ratios ...
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bioRxiv - Microbiology 2023Quote: ... biotinylated Pol II CTD mimic peptide (20 µg) were immobilised on 10 µl streptavidin agarose resin (Thermo Scientific) for 2 hours at 4 °C in binding buffer (10 mM HEPES [PAA catalogue no ...
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bioRxiv - Biophysics 2020Quote: ... 1 nmol of biotinylated 4-heptad CTD peptides (PeptideSynthetics) were incubated with 0.5 mg of Streptavidin-coupled magnetic Dynabeads M-280 (Invitrogen) in 300 µL of buffer A (25mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-Rpb1 8WG16 (Thermo Fisher MA1-10882, ChIP); anti-FLAG (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µg of non-phospho or phospho-CTD peptide (per condition) was conjugated to 10 µL (per condition) high-capacity streptavidin agarose beads (Cat#20357, Thermo Scientific) in peptide binding buffer (10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... the synthesized genes and the SOQ1 truncation containing NHL and CTD (SOQ1NHL-CTD) were PCR-amplified and cloned into pPICZB (Invitrogen) derivative vector which contains a tobacco etch virus (TEV ...
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bioRxiv - Neuroscience 2021Quote: ... CTD-2300P14 (Thermo Fisher Scientific, Supplier Item: 96012), labeled with Cy3 dUTPs (GE healthcare ...
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bioRxiv - Immunology 2021Quote: Sorted HSC were also cultured with blocking CD150 peptide (FCKQLKLYEQVSPPE, Auspep, 100 μg/ml) or control peptide (DLSKGSYPDHLEDGY, Auspep,100 μg/ml) (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Anti-Rpb1 and anti-Htz1 antibodies were coupled to protein A and protein G Dynabeads (Invitrogen) respectively for immunoprecipitation at 4°C ...
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bioRxiv - Biochemistry 2019Quote: WT and mutant CTD constructs were expressed in Escherichia coli BL21 Star DE3 (Invitrogen) cells in either LB media or ...
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bioRxiv - Cancer Biology 2021Quote: BAC clones RP11-467N14 (control locus) and CTD-2015C9 (ARL15 locus) were purchased (Thermofisher), whereas the HPV plasmid pSP64-HPV16 was prepared in house ...
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bioRxiv - Developmental Biology 2021Quote: ... After blocking with blocking buffers (Thermo Scientific) for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... MM cells and T cells were stained with Cell Trace Far Red dye (CTD, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... YFP-RPB1-26xCTD and T4E/S7E mutants were generated by PCR-based cloning performed by a kit from ThermoFisher. For HeLa cell transfection & imaging ...
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bioRxiv - Bioengineering 2019Quote: ... CTD-2207K13 (2207K13 BAC) and mouse BAC RP23-401D9 (ROSA BAC) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti-RNApII Rpb1 8WG16 antibody was coupled to Dynabeads coated with Pan Mouse IgG antibodies (Thermo Fisher Scientific, 11042). 50 μL of the appropriate Dynabeads pre-coupled with the indicated antibody were added to the chromatin sample and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and then blocking in Superblock Blocking Buffer (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed using BlockAid Blocking Solution (Thermo Fisher Scientific). Primary antibody incubation against clathrin heavy chain (ab21679 from Abcam ...
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bioRxiv - Plant Biology 2021Quote: The Trx-like domain and CTD were separately dialyzed in a 10 kDa dialysis card (Thermo Fisher) with the Buffer (25 mM Hepes pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Blocking Buffer (Invitrogen Cat# 00-4952-54 ...
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bioRxiv - Microbiology 2021Quote: ... and blocking in SuperBlock Blocking Buffer (Thermo Fisher Scientific, Waltham, MA). Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175 ...
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bioRxiv - Cancer Biology 2019Quote: ... After blocking with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific), the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... before blocking in SuperBlock™ blocking buffer (Thermo Fisher Scientific, 37515). Primary antibodies (MAP2 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... followed with endo-biotin blocking using a blocking kit (Invitrogen, #E21390) according to the manufacture instructions [118] ...
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bioRxiv - Microbiology 2023Quote: ... blocking was performed overnight using SEA BLOCK Blocking buffer (ThermoFisher, 37527) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations of eluted peptides were quantified using the Pierce Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific) and 5 µg of total peptides were labeled using TMTpro16-plex reagents (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were desalted with peptide desalting spin columns (Thermo Scientific) and dried via vacuum centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Biochemistry 2023Quote: Peptide samples were assayed for peptide concentration with Pierce™ Quantitative Colorimetric Peptide Assay (Thermofisher Scientific, Brisbane, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Labelled peptides were quantified (Pierce Colorimetric Peptide Assay Kit, Thermo Scientific) and mixed 1:1:1 based on peptide amount.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were quantified using a colorimetric peptide assay (Thermo Fisher Scientifc). Peptides (1 µg ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using colourimetric peptide assay (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using a colorimetric peptide assay (ThermoFisher Scientific). All samples were reconstituted in loading buffer (5% CH3CN ...
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bioRxiv - Biophysics 2021Quote: ... blocking of endogenous biotin was performed using a commercial blocking kit (Invitrogen, E21390). Cells were incubated in component A for 45 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... After blocking with 10% goat serum or Superblock blocking buffer (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Peptide digest assay standard (PierceTM Quantitative Colorimetric Peptide Assay, Thermo Scientific, USA) was as standard for quantitative analysis of peptides ...
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bioRxiv - Cell Biology 2022Quote: ... peptide concentration was measured using a colorimetric peptide quantification assay (Thermo Scientific). Finally ...
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bioRxiv - Systems Biology 2019Quote: ... Peptides were quantified using Pierce Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions on a Varioskan LUX Multimode Microplate Reader and SkanIt 5.0 software (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Peptide concentration was determined by a quantitative colorimetric peptide assay (Thermo Fisher), and 1 μg aliquots of clean peptides from each sample were injected sequentially over a nano-flow C18 column (EasySpray ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide concentrations were determined using Pierce Quantitative Fluorometric Peptide Assay (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... peptides were desalted on PierceTM Peptide Desalting Spin Columns (Thermo Fisher Scientific) and concentrated in a SpeedVac concentrator.
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bioRxiv - Microbiology 2021Quote: ... The peptide digests were then cleaned up using peptide desalting columns (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... After washing with PBS-T and blocking with StartingBlock (TBS) blocking buffer (ThermoFisher Scientific), serially diluted serum from groups of immunized mice or baboons were added to coated wells and incubated for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... followed by blocking with Starting Block T20 blocking buffer (Thermo Fisher Scientific catalog #37539) for 13 minutes ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... following peptide quantification (ThermoFisher). All samples were subjected to nanoLC-MS/MS analysis using an UltiMate 3000 RSLCnano system (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: Custom peptides (ThermoFisher Scientific) with the sequence N-CAIHAK115RVTIMPK-C were synthesized as unmodified (H3K115) ...