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Citations for Thermo Fisher :
1 - 50 of 762 citations for Ross River Virus VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: HIV-1 virus-like particles (VLPs) were produced by Lipofectamine 3000 (Thermo Fisher Scientific) transfection of 293FT cells (purchased from Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... with an Orion Ross Sure Flow Electrode (ThermoFisher Scientific), allowing up to 5 minutes for readings to stabilize.
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bioRxiv - Microbiology 2021Quote: ... glass double junction electrode (Orion™ PerpHecT™ ROSS™, Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... VLP-WT and VLP-cvD were expressed using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: VLP and scFc-VLPs (4µM) were applied onto a glow-discharged carbon-supported copper TEM grid (TEM-CF200CU50, Thermo Fisher Scientific) and incubated for 60 s before gently removing the solution ...
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bioRxiv - Microbiology 2023Quote: The pH of microbial cultures was measured by using a pH microelectrode (Orion PerpHecT ROSS; Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... RNA was extracted from the lysed VLPs using TriReagent (Ambion) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... VLPs were resuspended in Life cell imaging solution (Thermo Fisher) and stored at − 80°C ...
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bioRxiv - Microbiology 2020Quote: ... VLPs were incubated with AnnexinV-PE conjugate (AnV-PE) (Invitrogen) in AnV binding buffer for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The purified VLP fraction was treated with DNase I (Ambion) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... Pelleted VLPs were resuspended in 1X NuPAGE LDS sample buffer (ThermoFisher). Cell lysates were collected by washing cells twice with PBS followed by lysis in CytoBuster ...
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bioRxiv - Microbiology 2021Quote: ... Pelleted VLPs were resuspended in 1X NuPAGE LDS sample buffer (ThermoFisher). Cell lysates were collected by washing cells twice with PBS followed by lysis in CytoBuster (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were buffer exchanged into DPBS (Thermo Fisher Scientific Cat #: 14190-144) using a 100-kDa Amicon filter (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: The extracellular pH level of the culture medium was measured in 30 μl of fluid in which 20 embryos were cultured for 4.5 days by using a pH electrode with a small tip (3 mm in diameter, Orion Ross Electrode, Thermo Scientific, USA) attached to an Accumet basic AB15 pH meter (Mettler Toledo ...
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bioRxiv - Immunology 2019Quote: Chimeric VLPs or monomeric recombinant proteins were coated onto Maxisorp microtiter plates (Nunc) at 1μg/ml in PBS and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Enriched VLPs were lysed with 10% SDS and 20mg/mL Proteinase K (Ambion) at 56C° for 20 minutes ...
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bioRxiv - Microbiology 2019Quote: ... VLPs were produced using the Bac-to-Bac Baculovirus Expression System (Invitrogen, California, USA) and purified through a cesium chloride gradient as previously described (63) ...
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bioRxiv - Microbiology 2023Quote: Norovirus VLPs were labelled with an Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) dissolved in DMSO at a concentration of 10 µg/mL ...
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bioRxiv - Microbiology 2019Quote: ... VLPs were incubated for 2 hours in the DNAse and RNAse cocktails (Invitrogen, Carlsbad, CA) with the addition of 14 µL of 20 mg/ml Proteinase K (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were buffer exchanged into Dulbecco’s Phosphate Buffered Saline (DPBS) (Thermo Fisher Scientific #: 14190-144) using a 100-kDa Amicon filter (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... PV-3 SC8 VLPs or infectious PV-1 were incubated with 5 µM SYTO9 (Thermo Fisher) and SYPRO red (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... VLPs were expressed with the Bac-to-Bac TOPO Expression system (Invitrogen, Catalog nos. A11101, A11100) according to instructions from the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Stained VLPs were image with Talos L120 transmission electron microscope (TEM, Thermo Fisher Scientific, former FEI) with 47000x magnification ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Physiology 2021Quote: ... Blood was drawn from the caudal vein using a heparinized syringe and pH was immediately measured using a microelectrode (Orion™ PerpHecT™ Ross™, ThermoFisher Scientific, Waltham, MA, USA). Next ...
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
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bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
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bioRxiv - Immunology 2023Quote: ... PCSK9 peptides were conjugated to Qß bacteriophage VLPs using succinimidyl 6-((ß-maleimidopropionamido)hexanoate) (SMPH; Thermo Fisher Scientific), as described previously60 ...
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bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: VLPs were produced in adherent HEK-293 [HEK-293](ATCC-CRL-1573) or the suspension Expi293 cells Expi293 (Gibco). HEK-293 cells were maintained in 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Immunology 2023Quote: Proteins in VLP containing samples were separated by SDS-PAGE using 4-12% Bis-Tris Nu-PAGE gels (Invitrogen) and electro-transferred to a PVDF membrane using the Trans-Blot Turbo Transfer Pack (BioRad) ...
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bioRxiv - Immunology 2024Quote: ... The protein concentration of the purified VLPs was measured using a Pierce™ BCA Protein Assay kit (Thermo Fisher). VLPs were then flash frozen in liquid nitrogen and stored at −80 °C until use ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Pelleted virus was resolved in PBS (Gibco) and stored at −80°C until further usage ...
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bioRxiv - Immunology 2019Quote: ... murine leukemia virus reverse transcriptase (RT; Invitrogen), and random hexamers (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Fibroblasts were reprogrammed using Sendai virus (Invitrogen) in feeder-free conditions on Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... and EmGFP reporter Sendai Virus (A16519, Invitrogen) were part of the Cytotune -iPS 2.0 Sendai Reprogramming kit and EmGFP Sendai Fluorescence Reporter kit ...
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bioRxiv - Immunology 2024Quote: ... in virus growth medium (1X MEM [Gibco 11095080] ...
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bioRxiv - Bioengineering 2023Quote: Concentrated EV or VLP total protein concentrations were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific, USA) These neutralization reagents were mixed with 4X loading dye ...
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bioRxiv - Microbiology 2024Quote: ... HEK293FT (293T cells optimized for VLP production, gift from N. Manel) and OMK cells were cultivated in media DMEM (Thermofisher) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: The ESP collector surface for VLP deposition was modified to integrate two 50 μL collection wells (Thermo Fisher Scientific AB0620). The wells were metallized with gold using a sputter deposition system (CVC SC-4000 RF Magnetron Sputtering System ...
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bioRxiv - Biophysics 2023Quote: ... the solution of stained VLP was buffer exchanged with pure PBS using Zeba™ Spin Desalting Columns (7,000 MWCO, ThermoFisher). We note that since the columns contained sodium azide ...
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bioRxiv - Microbiology 2020Quote: ... or P1 SARS-CoV-2/München- 1.1/2020/929 (Munich) virus was diluted to 500 µl in 15 ml with virus dilution medium (Opti-MEM, Gibco) supplemented with 2% (v/v ...