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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Collagen Type 1 alpha 1 (COL1A1) (ThermoFisher Sci, catalog No. PA5-29569), smooth muscle α-actin (αSMA ...
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bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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bioRxiv - Developmental Biology 2019Quote: ... anti-Collagen Type II (Thermo Scientific, MS235B, 1:100), anti-Collagen Type X (Quartett ...
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bioRxiv - Microbiology 2021Quote: ... Human alpha-1 PDX (alpha1-PDX) recombinant protein was purchased from ThermoFisher Scientific.
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bioRxiv - Cancer Biology 2024Quote: ... in collagen-treated plates (Collagen Type 1, Gibco). Cells were incubated with experimental compounds for 48 hours at 37 °C and 5% CO2 in a humidified incubator ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant 6x-His tagged PTB was purified using Ni-NTA agarose (Invitrogen) according to manufacturer’s instructions ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... previously coated with human type I collagen (Gibco, A1048301). ALI was established once cells reached full confluency by removing media from the apical chamber and replacing media in the basolateral chamber with Pneumacult-ALI maintenance medium (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2019Quote: Purified recombinant human p38α (MAPK14, GST-tagged, Thermo Fisher Scientific, #PV3304), p38β (MAPK11 ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant GST-tagged kinase active human mTOR (Cat. PV4753, ThermoFisher) was used instead of the immunoprecitated mTORC1 ...
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bioRxiv - Cell Biology 2023Quote: Type 1 rat-tail collagen (Life Technologies) was used at a stock concentration of 3 or 10 mg mL-1 ...
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bioRxiv - Microbiology 2021Quote: ... or a type IV DQTM collagen conjugate from human placenta (Invitrogen). Reactions were prepared in technical triplicate or quadruplicate by mixing 20 µL of substrate at 0.25 mg/mL with 80 µL of reaction buffer and 100 µL of bacterial suspension ...
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bioRxiv - Bioengineering 2023Quote: ... / type-I collagen (Gibco™ collagen type I, rat tail, ThermoFisher) matrix with a 1:1:2 volume ratio ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... on plates coated with recombinant human collagen I (Coating Matrix kit, Gibco) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... / type-I collagen (Gibco™ collagen type I ...
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bioRxiv - Microbiology 2022Quote: ... (S)-MG132 was purchased from Cayman Chemicals (10012628) and Type 1 Collagen from ThermoFisher (A1048301).
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Genomics 2019Quote: ... collagen type I (Thermofisher Scientific) was incubated in the channels for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-Collagen II (Thermo Scientific MS235B, 1:100), anti-GPCR GPR126 (ADGRG6 ...
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ST6GAL1-mediated heterogeneity in sialic acid levels potentiates invasion of breast cancer epitheliabioRxiv - Cancer Biology 2020Quote: ... or 50 μg/mL neutralized rat tail collagen (rich in type 1 collagen) (Gibco, A1048301) for 2 hours at 37 °C ...
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bioRxiv - Bioengineering 2022Quote: ... collagen II (MA512789; Thermofisher), or collagen X (14-9771-82 ...
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bioRxiv - Physiology 2023Quote: ... Collagen Type I Polyclonal Antibody (Thermo Scientific 14695-1-AP), COLIV rabbit polyclonal (Assay Biotech C0157) ...
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bioRxiv - Cell Biology 2019Quote: ... A collagen gel containing 2% type-I DQ-collagen (Molecular Probes) was used for the DQ-collagen degradation assay.
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
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bioRxiv - Immunology 2019Quote: ... pre-coated with 50 μg/ml type 1 collagen (Thermo Fisher) in 0.02 M acetic acid ...
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bioRxiv - Microbiology 2023Quote: ... whereas His-tagged EF-P was detected with primary monoclonal antibodies against the His-Tag (1:10,000; AB_2536841, Invitrogen). Membranes were incubated with the secondary antibodies conjugated with a fluorophore (1:20,000 in TBS ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
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bioRxiv - Cell Biology 2019Quote: ... p38β (MAPK11, His-tagged, Thermo Fisher Scientific, #PV3679), p38γ (MAPK12 ...
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bioRxiv - Cell Biology 2019Quote: ... p38γ (MAPK12, His-tagged Thermo Fisher Scientific, #PV3654), p38d (MAPK13 ...
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bioRxiv - Cell Biology 2019Quote: ... p38d (MAPK13, His-tagged, Thermo Fisher Scientific, #PV3656) and hPI31 (UBPBio ...
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bioRxiv - Immunology 2023Quote: ... 6x His Tagged monoclonal antibody (Thermo Fisher Scientific) was also plated as an experimental control ...
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bioRxiv - Microbiology 2021Quote: ELISA assays were performed in 96-microwell plates coated with 150 ng/well of SARS-CoV-2 Spike (aa 16-1213) His-tagged recombinant protein (ThermoFisher) and 1010 phage or AAVP particles/50 μL of PBS O.N ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then purified by immobilized metal affinity chromatography by using HisPur Ni-NTA (Purify His-tagged recombinant fusion proteins using high-capacity nickel-nitrolotriacetic acid methal-chelate beads) spin columns (ThermoFisher). Recombinant proteins were refolded on the column by gradually removing urea until reaching to a concentration 0 M and then dialyzed against PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... collagen I in 0.1% acetic acid (Collagen, rat tail, Type I, Invitrogen) was injected via the inlet of the cell-culture chamber ...
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bioRxiv - Cancer Biology 2021Quote: ... DQ™-collagen type I (Life Technologies/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... (Collagen type I, Thermofisher, Cat No - 1048301). Images were acquired 48h later and gel contraction was quantified with Image J.
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bioRxiv - Bioengineering 2020Quote: ... the collagen solution was prepared by mixing 50% (v/v) of 3 mg/mL type 1 collagen (ThermoFisher) with 2.5% of 1N NaOH ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
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bioRxiv - Cell Biology 2021Quote: ... a collagen sheet containing 2% DQ-collagen type I (D12060, Thermo Fisher Scientific) was used ...
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bioRxiv - Microbiology 2022Quote: 1mg/ml type-I collagen gels were made from rat tail collagen (Gibco) as previously described in a 96-well plate (Schuh et al ...
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bioRxiv - Neuroscience 2022Quote: ... 10μg ml−1 human recombinant insulin (GIBCO, 12585014).
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bioRxiv - Cell Biology 2019Quote: ... pretreated with type I collagen (Gibco A10483-01) to promote cell adherence ...
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bioRxiv - Cell Biology 2021Quote: Purified recombinant GST-tagged CDK1-cyclin A2 and His6-tagged CDK1-cyclin B1 (Invitrogen) were stored in 150 mM NaCl ...
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bioRxiv - Systems Biology 2020Quote: ... the GST-Tagged proteins were eluted with 15 mM GSH (Bio-basic) and cleaved in solution using purified recombinant His-Tagged TEV protease (Thermo Fisher Scientific). Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare ...