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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Recombinant Human Angiopoietin 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant 6x-His tagged PTB was purified using Ni-NTA agarose (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ELISA assays were performed in 96-microwell plates coated with 150 ng/well of SARS-CoV-2 Spike (aa 16-1213) His-tagged recombinant protein (ThermoFisher) and 1010 phage or AAVP particles/50 μL of PBS O.N ...
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bioRxiv - Cell Biology 2019Quote: Purified recombinant human p38α (MAPK14, GST-tagged, Thermo Fisher Scientific, #PV3304), p38β (MAPK11 ...
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bioRxiv - Cell Biology 2022Quote: ... the recombinant GST-tagged kinase active human mTOR (Cat. PV4753, ThermoFisher) was used instead of the immunoprecitated mTORC1 ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: ... and the human angiopoietin-like 4 protein (ANGPTL4) ELISA Kit were purchased from Invitrogen-ThermoFisher Scientific (Carlsbad ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant IL-2 (2 ng/ml) (Gibco, USA), IL-7 (10 ng/ml ...
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bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Microbiology 2023Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured in RPMI-1640 supplemented with 10% HI-FBS and fed with recombinant human IL-2 (ThermoFisher) and IL-15 (Miltenyi ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... Recombinant human IL-2 was purchased from ThermoFisher Scientific.
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bioRxiv - Microbiology 2022Quote: ... and 50 IU/mL recombinant human IL-2 (Gibco). IL-2 was also supplemented to each culture for the maintenance of the cells.
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bioRxiv - Neuroscience 2024Quote: ... After blocking supernatant samples from different groups and standards (recombinant human-TREM2-His; Life Technologies) were incubated for 2 h at room temperature (RT ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His-tagged constructs of human and murine SNED1 cloned into pCDNA5/FRT (Thermo Fisher, Waltham, MA) between the FseI and AscI sites were used to transiently transfect 293T cells to validate the anti-SNED1 antibody generated in this study (Supplementary Figure S1) ...
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bioRxiv - Cell Biology 2019Quote: ... p38β (MAPK11, His-tagged, Thermo Fisher Scientific, #PV3679), p38γ (MAPK12 ...
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bioRxiv - Cell Biology 2019Quote: ... p38γ (MAPK12, His-tagged Thermo Fisher Scientific, #PV3654), p38d (MAPK13 ...
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bioRxiv - Cell Biology 2019Quote: ... p38d (MAPK13, His-tagged, Thermo Fisher Scientific, #PV3656) and hPI31 (UBPBio ...
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bioRxiv - Immunology 2023Quote: ... 6x His Tagged monoclonal antibody (Thermo Fisher Scientific) was also plated as an experimental control ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then purified by immobilized metal affinity chromatography by using HisPur Ni-NTA (Purify His-tagged recombinant fusion proteins using high-capacity nickel-nitrolotriacetic acid methal-chelate beads) spin columns (ThermoFisher). Recombinant proteins were refolded on the column by gradually removing urea until reaching to a concentration 0 M and then dialyzed against PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: 1-2 μg His-tagged calcineurin was first bound to magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
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bioRxiv - Cell Biology 2021Quote: Purified recombinant GST-tagged CDK1-cyclin A2 and His6-tagged CDK1-cyclin B1 (Invitrogen) were stored in 150 mM NaCl ...
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bioRxiv - Systems Biology 2020Quote: ... the GST-Tagged proteins were eluted with 15 mM GSH (Bio-basic) and cleaved in solution using purified recombinant His-Tagged TEV protease (Thermo Fisher Scientific). Tag-free NCK2 proteins were further purified using a 1 ml HisTrap FF column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2.5 ng/mL of recombinant human IL-2 (Thermofisher, PHC0027). T-cells were co-cultured with the mCherry-Nucleus-7 MCF7 cells in the presence of CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 5 ng/mL recombinant human interleukin 2 (Gibco, #PHC0027), 2 mM L-glutamine (Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 ng/ml recombinant human FGF-Basic (Thermo Fisher Scientific) in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Immunology 2021Quote: ... with 17 ng/ml of recombinant human IL-2 (ThermoFisher Scientific). MART-1-specific CD8+ T-cell clones were generated by Friedmann et al (Friedmann et al. ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged ChElp2 was pulled-down with Dynabeads™ His-Tag (Thermo Fisher Scientific, Massachusetts, USA) and the subsequent western blot analyses were performed with anti-His (Sigma ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Cell Biology 2023Quote: ... human recombinant fibroblast growth factor 2 (FGF2, 20 ng/mL, Life Technologies), and beta-mercaptoethanol (0.1% ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... for His-tagged proteins or GST-agarose resin (Thermo Fisher Scientific) for GST-tagged proteins ...
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bioRxiv - Microbiology 2021Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or SARS-CoV-2 N-His (Sino Biological # 40588-V08B ...
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bioRxiv - Microbiology 2023Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or HCoV-OC43 N-His (Sino Biological # 40643-V07E ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged Gβγ was expressed in Sf9 cells for 2 days following baculovirus infection (pFastBacDual, Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: His-tagged P-Rex2 was expressed in Sf9 cells for 2 days following baculovirus infection (Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...