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Citations for Thermo Fisher :
1 - 50 of 7790 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Anti-TUT1 (PA5-50151) was obtained from Invitrogen.
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bioRxiv - Neuroscience 2023Quote: ... cultured rat astrocytes were transfected with shRNA and/or expression plasmids using Lipofectamine LTX with Plus Reagent (Thermo Scientific) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: COL11A1 shRNA plasmid was obtained from NIEHS Genomic core (Thermo Scientific GIPZ Lentiviral shRNA). Lentiviral packaging was produced at the NIEHS Viral Vector Core ...
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bioRxiv - Molecular Biology 2020Quote: ... we transfected cells with shRNA plasmids or RFP-GFP-[] plasmids using Lipofectamine 2000 (Thermofisher 11668-027) and the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were plated in 24-well plates at 5 x 104 per well and cotransfected with 0.1 μg bicistronic plasmids along with either 0.5 μg control shRNA or anti-eIF2D shRNA using Lipofectamine LTX (Thermo Fisher Scientific). The cells were cultured for 48h in DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were planted in 6-well plates at 2 x 105 per well and cotransfected with 0.5 μg bicistronic plasmids along with either 2.5 μg control shRNA or anti-eIF2D shRNA using Lipofectamine LTX (Thermo Fisher Scientific). For luciferase assays (see below) ...
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bioRxiv - Cell Biology 2021Quote: ... 6 ng/well) and shRNA-containing transfer (pLKO.1-shRNA, 50 ng/well) plasmids were combined in 20 μl/ well OptiMEM (Life Technologies) containing 0.2 μg/well linear polyethyleneimine 25,000 (PEI ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were plated in 24-well plates at 5 × 104 per well and cotransfected with 50 ng of the AS-C9 plasmids and 50 ng of the fLuc plasmids along with 500 ng of either control shRNA or anti-eIF2D shRNA using Lipofectamine LTX (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... along with shRNA plasmids (8 μg) using Lipofectamine 2000 (Thermo Fisher Scientific) transfection reagent ...
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bioRxiv - Molecular Biology 2022Quote: ... and transfected with plasmid or shRNA using Lipofectamine 2000 (11668-019, Invitrogen) and opti-MEM (31985070 ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T cells were transfected with shRNA or gRNA plasmid and VSVG packaging plasmids mixture using lipofectamine 2000 (ThermoFisher Scientific). Lentiviral supernatants were collected at 48-72 h later and kept frozen at −80°C until needed ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were transfected with shRNA plasmid and helper plasmids psPAX2 and pMD2.G using Opti-MEM (Invitrogen #22600-050) medium and lipofectamine (https://www.thermofis.com/order/catalog/product/12566014Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK-293T cells were transfected with shRNA plasmid and helper plasmids psPAX2 and pMD2.G using Lipofectamine 2000 (Invitrogen #11668027) in Opti-MEM (Invitrogen #22600-050 ...
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bioRxiv - Cell Biology 2020Quote: ... 13 μg gene-specific lentiviral shRNA plasmids and 135 μl of PLUS reagent (Thermo Fisher) were added to 4 ml Opti-MEM as mixture A ...
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bioRxiv - Cell Biology 2020Quote: shRNAs were designed using the online shRNA design tool from Invitrogen (http://rnaidesigner.thermofisher.com/rnaiexpress/setOption.do?designOption=shrna&pid=5230999276329139407/ ...
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bioRxiv - Molecular Biology 2020Quote: ... and shRNA plasmids or pCDH plasmids by PEI-40000 with the ratio of 5: 1: 5 in opti-MEM (Gibco, Thermo Fisher Scientific). Virions were collected after 48 h after transfection ...
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bioRxiv - Molecular Biology 2020Quote: Plasmids containing shRNA molecules against SCD and SREBP1 genes were isolated by using Gene JET Plasmid Miniprep Kit (#K0503, Thermo Scientific, USA). The plasmid obtained from each pENTR™/U6 entry construct was sequenced to confirm the sequence and correct orientation of the ds oligo insert.
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bioRxiv - Physiology 2021Quote: ... MEFs were transfected with plasmids encoding for Nrf1-kd shRNA (see below) using Lipofectamine 2000 (Invitrogen). 48 hours after transfection cells were scraped and lysed in ice cold RIPA buffer (50 mM TrisHCl pH 8.0 ...
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bioRxiv - Bioengineering 2019Quote: ... and one of the pLKO.1 shRNA plasmids using 5:1 lipofectamine 2000 (Thermo Fisher Scientific):DNA (v/w ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 nM (final concentration) shRNA or 3 ∼ 5 µg plasmid in 50 µL Opti-MEM (Gibco) was mixed with 5 µL transfection reagent (Lipofectamine ...
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bioRxiv - Molecular Biology 2020Quote: ... and shRNA plasmids or pCDH plasmids by PEI-40000 with the ratio of 5: 1: 5 in opti-MEM (Gibco, Thermo Fisher Scientific). Virions were collected after 48 h after transfection ...
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bioRxiv - Immunology 2022Quote: ... 2.7 μg envelope plasmid pMD2G and 8 μg target plasmid encoding shRNA or lentiCRISPRv2 encoding sgRNA using Lipofectamine® 2000 (Thermo Fisher Scientific). Lentivirus supernatants were collected at 36 h ...
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bioRxiv - Neuroscience 2023Quote: ... The shRNA oligos (ThermoFisher) were annealed and subcloned into pLKO-TRC vector between the two restriction sites ...
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bioRxiv - Neuroscience 2020Quote: GFP reporter plasmids (eGFP, PLL3.7 shRNA) were transfected into neurons at DIV 14 using Lipofectamine 2000 (Invitrogen). LNA Gapmers targeting ADEPTR or siRNAs targeting kinesins were transfected using Lipofectamine RNAiMax (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids and shRNAs were transfected by the calcium phosphate method or with Lipofectamine 2000 (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 293T cells were cotransfected with the shRNA and packaging plasmids psPAX2 and pMD2 using Lipofectamine 2000 (Invitrogen) in OptiMEM (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... 1 µg of shRNA-expressing plasmids were used to transfect cells with Lipofectamine 2000 (Thermo Fisher Scientific, USA). After 24 h of transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral DR5 shRNA constructs were from the TRC Lentiviral shRNA Libraries from Thermo Scientific. Labeling cells with Firefly Luciferase (fLuc ...
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bioRxiv - Cancer Biology 2021Quote: Stable cell lines were performed by co-transfecting HEK293-FT packaging cells with a pLKO.1-puromycin shRNA plasmid with VSVG and SPAX2 lentiviral packaging vectors using Lipofectamine 2000 according to the manufacturer’s instructions (Invitrogen). Viral supernatants were collected ...
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bioRxiv - Cancer Biology 2019Quote: shRNA sequences for ITGAV (integrin αv) and CD44 were obtained as glycerol stock in pTRIPZ inducible plasmids (Thermo Scientific). For transduction ...
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bioRxiv - Neuroscience 2019Quote: Silencing lentiviral vectors were produced by co-transfecting HEK293T producing cells with lentiviral silencing plasmids GIPZ Human histamine H3 receptor shRNA (Clone V3LHS_638095 or Clone V3LHS_638091, Thermo Scientific) with packing plasmid psPAX2 and envelope coding plasmid pMD2.G (Addgene#12260 and #12259 ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfection of plasmids or shRNAs into MSCs was conducted by using a Lipofectamine 3000 system (#1687583; Invitrogen, Carlsbad, CA). Efficiency of genetic OE or KD was verified according to the manufacturers’ instructions.
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bioRxiv - Cancer Biology 2023Quote: ... were mixed with a lentiviral plasmid containing a gene of interest or shRNA and incubated with Lipofectamine 3000 (Invitrogen) in serum free Opti-MEM (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... Cells at 60-70% confluency were transiently transfected with plasmids or shRNAs using Lipofectamine 3000 according to the manufacturer’s protocol (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... the VSV-G envelope protein vector pMD2.G and the respective pGIPZ-shRNA-target plasmid using lipofectamine 3000 (Invitrogen #L3000008). Filtered virus supernatant was used to transduce MDA-MB-468 and 4T1 cells in DMEM supplemented with 2% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and transfer plasmid (N174-CMV-GFP-miR-E-IRES-puro-shRNA) into HEK293T cells with Lipofectamine 2000 reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and pVSV.G (6.5 μg/ml) and an expression or shRNA plasmid (12 μg/ml) were diluted in OPTI-MEM media containing lipofectamine 2000 (Invitrogen™) and co-transfected into HEK-293T cells ...
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bioRxiv - Neuroscience 2019Quote: Full-length rat GluA1 and GluA2 cDNAs were subcloned into pcDNA3 (plasmid vector, Invitrogen) to generate pc3-GluA1 and pc3-GluA2 ...
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bioRxiv - Cancer Biology 2021Quote: ... shRNA cells were cultured using RPMI (Gibco) supplemented with 10% fetal bovine serum and 1% antibiotic antimycotic ...
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bioRxiv - Immunology 2022Quote: ... Retrovirus containing a control shRNA (firefly luciferase) or the Ppargc1b shRNA were produced by 293T cells transfected using Lipofectamine 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... either 3.6 µg of scramble shRNA or MAP6 shRNA were transfected into hippocampal neurons using Lipofectamine 2000 transfection reagent (Invitrogen, 11668030). Neurons were then collected for protein analysis using western blot.
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bioRxiv - Cancer Biology 2020Quote: For depletion of RB1CC1 virus particles were generated in HEK 293-T cells transfected with the control vector pGIPZ or the respective shRNA plasmid (KD1: RHS4430-101100096 or KD2: RHS4430-101103887) (Thermo Scientific Open Biosystems), the gag/pol plasmid pCMV-dr8.91 and the VSV-g envelope plasmid pMD2.G (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... All siRNAs and shRNAs were from Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Neuroscience 2019Quote: ... and shRNA using Lipofectamine 2000 (Invitrogen, CA, USA). Lentiviral particles collected from culture medium were isolated and concentrated according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and pLKO.1 shRNA using Lipofectamine 3000 (Invitrogen). Cells were incubated with viral supernatant containing polybrene (8 mg/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... and the target shRNA using Lipofectamine 2000 (Invitrogen). The following day ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were either transduced with lentiviral particles carrying a control shRNA or a specific shRNA against RTP801 or transfected with lipofectamine 2000 (Thermo Fisher Scientific) with pCMS vectors expressing eGFP (donated by Dr ...
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bioRxiv - Microbiology 2021Quote: ... and sgRNA or shRNA using Lipofectamine 2000 (Thermo Fisher). Lenti-X Concentrator (TaKaRa #631232 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with pLKO.1 puro vector constructs expressing shRNA against mouse Aldh1a1 or Alhd1a3 and non-silencing control shRNA using Lipofectamine 2000 Transfection Reagent (Thermo Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cultured hippocampal neurons treated with adenoviruses expressing either βII-spectrin shRNA or scrambled (control) shRNA were lysed on ice using the RIPA buffer (Thermo Fisher Scientific, 89900) complemented with 1x protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...