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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for RNA Binding Motif Protein 45 RBM45 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... a Tet-inducible EGFP-RBM45 construct was generated by inserting the EGFP-RBM45 (N-terminal EGFP fusion) into the pcDNA4/TO vector (Invitrogen). The sequences of all constructs were confirmed by Sanger DNA sequencing and the sizes of the expressed proteins confirmed by Western blot.
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bioRxiv - Cell Biology 2022Quote: ... Binding of indicated antibodies to Dynabeads Protein A (Invitrogen) was performed overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Binding of indicated antibodies to Dynabeads Protein A (Invitrogen) was performed overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA binding proteins were isolated using Pierce™ Magnetic RNA-Protein Pull-Down Kit (ThermoFisher, #20164). Briefly ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of the xopX gene and its 14-3-3 protein binding motif mutants were cloned in the yeast two-hybrid vector pDEST32 (Invitrogen) using the Gateway cloning system (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... RNA binding proteins were isolated by the means of Pierce™ Magnetic RNA-Protein Pull-Down Kit (ThermoFisher, #20164). Briefly ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of xopX and its 14-3-3 protein binding motif mutants were cloned by Gateway cloning (Invitrogen, California) from the pENTR clones to the BiFC vector pDEST-VYCE(R)GW carrying the C-terminal region of the Venus Fluorescent Protein (VFP ...
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bioRxiv - Cell Biology 2020Quote: ... PAG1 (Csk-binding protein antibody PAG-C1) 1:1000 (ThermoFisher #MA1-19287); Phospho-Src family (Tyr416 ...
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bioRxiv - Cancer Biology 2024Quote: ... Nonspecific antibody binding was blocked using Ultravision Protein-block (Thermo Fisher Scientific). Next ...
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bioRxiv - Immunology 2023Quote: ... protein-binding plates (Nunc Maxisorp) were coated with rMOG (housemade ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein binding was detected with Alexa488-conjugated α-GST antibody (Invitrogen A-11001). Arrays were scanned using a GenePix 4400A microarray scanner (Molecular Devices) ...
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bioRxiv - Cancer Biology 2024Quote: ... and nonspecific antibody binding was blocked using Ultravision Protein-block (Thermo Fisher Scientific). Sections were incubated overnight with rat anti-B220 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... chromatin was sheared and antibody-conjugated (H3K27ac active motif: 39133) Protein A Dynabeads beads (Invitrogen) were used for chromatin extraction ...
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bioRxiv - Cell Biology 2023Quote: ... Bead preparation and antibody binding: 30-50 µL of DynabeadsTM Protein G (Invitrogen, 10003D) were used per ChIP ...
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bioRxiv - Microbiology 2023Quote: ... or no protein in 200 µl antibody binding and washing buffer (Invitrogen kit 10006D) for one hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... The antibody-binding proteins were pulled down using protein A conjugated magnetic beads (Thermo Scientific, Cat No.88845) and washed by repeated centrifugation and homogenization ...
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bioRxiv - Microbiology 2021Quote: ... A mouse monoclonal anti-HSV 45-kDa protein (scaffolding protein) antibody (Serotec) at 1:2000 dilution and Alexa Fluor 568–goat anti-mouse IgG (Invitrogen) at 1:1000 dilution were used as primary and secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... placed in a low binding tube (Low Protein Binding Microcentrifuge tubes, Thermo Scientific). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... High protein-binding MaxiSorp plates (ThermoFisher Scientific) were coated with 200 ng/well soluble LCMV sGP resuspended in 1x TBS and incubated overnight at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein binding was detected with an Alexa488-conjugated anti-GST antibody (Life Technologies A-11131), and arrays were scanned using a GenePix 4400A (Molecular Devices ...
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bioRxiv - Physiology 2022Quote: ... 18S ribosomal RNA (18S) and TATA-binding protein (Tbp).The primers (Thermofisher Scientific, Waltham, MA, USA) were derived from mouse sequences and are listed in supplemental methods (supplement table 2.).
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bioRxiv - Biochemistry 2023Quote: ... Mouse α-TATA Binding Protein (ThermoFisher 49-1036), Goat α-mouse:HRP (Novus Biologicals NBP2-31347H) ...
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bioRxiv - Neuroscience 2022Quote: JD1 antibody was adsorbed to high protein binding Nunc-Immuno MaxiSorp 96-well plates (Nunc, Roskilde,) at 4°C for 4hrs ...
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bioRxiv - Cell Biology 2024Quote: ... Antibody binding was followed by incubation with Protein A/G magnetic beads (Thermo Fisher Scientific, USA) for 1h at 4°C on a rotator ...
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bioRxiv - Microbiology 2023Quote: ... 0.4pmol of biotinylated SINV RNA was incubated with normalized amounts of protein lysates and RNA binding buffer containing RNaseOUT (Thermo Fisher), heparin (Sigma-Aldrich) ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: NUCKS1’s GRP DNA-binding motif was mutated to AAA using the Phusion Site-Directed Mutagenesis Kit (Thermofisher), with primers as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 45 min incubation with Protein G beads (Invitrogen). The complex was washed four times in ice cold PBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by binding of antigen antibody complexes to protein A/G magnetic beads (Thermo scientific, Rockford, USA) for 1 h ...
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bioRxiv - Microbiology 2023Quote: DB1 antibody was purified by binding clarified cell supernatant to Protein A Agarose beads (Thermo Fisher Scientific), with elution into 100 mM glycine pH 3.0 and was quickly neutralised with 1 M Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in polyclonal anti-RBPMS (RNA Binding Protein Multiple Partners; 1:1000; ThermoFisher, CAT: PA5-31231) at 4ºC ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by binding to Protein-G Dynabeads (Invitrogen, 10003D) for 20 minutes hours at 4°C to enrich for endothelial cell nuclei ...
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bioRxiv - Immunology 2024Quote: ... Pierce protein G IgG binding buffer (Thermo Fisher Scientific) was used to dilute the serum 1:1 before loading as well as for equilibration and wash steps ...
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Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse StriatumbioRxiv - Neuroscience 2022Quote: ... anti-H3K27me3 (Antibody 1: Active Motif, 39055; Antibody 2: Thermo Fisher, MA5-1198) or IgG (Epicypher ...
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bioRxiv - Cell Biology 2020Quote: ... which were incubated overnight at 4°C with an anti-OxPhos antibody cocktail (Invitrogen, #45-8099, #45-7999), and were analyzed using the Western Breeze Chromogenic Western Blot Immunodetection Kit (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... streptavidin biotin-binding protein (Streptavidin Alexa 488, 1:500, Invitrogen) was diluted in 5% BSA with 5% NGS and 0.3% Triton-X overnight at 4 °C ...
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Complement-associated loss of CA2 inhibitory synapses in the demyelinated hippocampus impairs memorybioRxiv - Neuroscience 2021Quote: ... Streptavidin biotin-binding protein (Streptavidin Alexa 488, 1:500, Invitrogen) was diluted in 5% BSA with 5% NGS and 0.3% Triton-X overnight at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: High-protein binding 96-well microplates (3855, ThermoFisher, MA, US) were coated overnight with 10 µg/ml of either porcine collagen II (20031 ...
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bioRxiv - Bioengineering 2024Quote: ... and the mouse monoclonal antibody (17TF2-1H4) specific for the bacterial maltose-binding protein (anti-MBP, #MA3045 Fisher Scientific) were purified as described,[34] with minor modifications ...
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bioRxiv - Cell Biology 2023Quote: ... OxPhos Rodent WB Antibody Cocktail (1:250, #45-8099 Invitrogen) and β-actin (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... OxPhos Human WB Antibody Cocktail (Thermo-Fisher Scientific #45-8199) and β-actin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and OxPhos Human WB Antibody Cocktail (Thermo Fisher 45-8199). Detection of protein signals was performed using ECL Prime Western Blotting Detection Reagent (Cytiva RPN2236 ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from purified astrocyte preparations using RNA-binding columns (PureLinkTM RNA Micro Kit, Invitrogen), followed by RNA quality assessment (2100 Bioanalyzer system ...
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bioRxiv - Cell Biology 2020Quote: ... The extracts were incubated at 70 °C for 45 min and used for protein analysis or RNA isolation using Trizol LS (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... and low protein binding microcentrifuge tubes were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2020Quote: ... To reduce nonspecific binding protein G magnetic beads (DynaBeads, Thermo Fisher) were added to the lysate and incubated for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and collected in low protein binding microcentrifuge tubes (Thermo Scientific, 90410). Cells were pelleted by centrifugation at 1 000 x g for 5 min at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Microbiology 2022Quote: Virus particle alterations were characterized by a thermofluorometric dual dye-binding assay using the nucleic acid dye SYTO9 and the protein-binding dye SYPRO orange (both from Invitrogen). Reaction mixtures of 50 μl containing 1.0 μg of purified virus ...
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bioRxiv - Physiology 2019Quote: ... Blots were incubated with OxPhos antibodies (Thermofisher 45-8099 1:1000), UCP-1 (Abcam ab10983) ...