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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... and protein digestions were conducted using an In-Solution Tryptic Digestion and Guanidination Kit (89895, ThermoFisher Scientific)‡ ...
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bioRxiv - Developmental Biology 2022Quote: ... The isolated proteins were then prepared for mass spectrometry using an in-solution protein digestion kit (Thermo Scientific). In brief ...
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bioRxiv - Molecular Biology 2020Quote: ... The distinct protein bands were excised and were subjected to in-gel trypsin digestion using In-Gel Tryptic Digestion Kit (Thermo Scientific, USA). The recovered peptides in digestion buffer were dried under vacuum and dissolved in 0.1% Trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2020Quote: ... as measured on protein level (BCA protein concentration determination kit; before digestion) was labeled with 10-plexing tandem mass tags (TMT-10; Thermo Scientific) following the manufactures instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and column DNase digestion kit (Life Technologies). The cDNA synthesis ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed in digestion buffer by addition of 5 µg Pierce MS grade Trypsin (Thermo Fisher Scientific) at 37°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... and treated with a DNA Digestion Kit (Ambion) to remove DNA ...
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bioRxiv - Systems Biology 2020Quote: ... The proteins were acetone precipitated prior to digestion with recombinant trypsin (ThermoFisher) (1:50 wt/wt. ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by protein digestion with 400 μg/ml of proteinase K (Invitrogen) overnight at 55°C ...
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bioRxiv - Immunology 2021Quote: Protein Digestion and Tandem Mass Tag (TMT) labeling: The Pierce detergent compatible Bradford assay kit from Thermo Fisher (Winnipeg, MB) was used to determine the protein concentration ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 2 μg protein per sample was digested using trypsin beads in accordance with the manufacturer’s protocol (SMART digestion kit, Thermo Scientific). Supernatants were extracted from the beads and 10% formic acid added to adjust to pH 3 ...
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bioRxiv - Synthetic Biology 2020Quote: ... An additional digestion using Turbo DNA-free Kit (Invitrogen) was performed after purification to eliminate any remaining genomic DNA ...
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bioRxiv - Genomics 2019Quote: ... Digestion products were purified with the Gel Extraction Kit (Thermofisher) from 1% agarose gels ...
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bioRxiv - Bioengineering 2023Quote: ... DNAse digestion was performed using Turbo DNA-free kit (Invitrogen) according to manufacturer’s instructions to remove potential plasmid contamination ...
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bioRxiv - Cancer Biology 2023Quote: ... Digestion mix consisted of Liver Digestion Medium (ThermoFisher) with 1% HEPES and 700U/mL Collagenase type IV (Worthington Biochemical) ...
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bioRxiv - Microbiology 2021Quote: ... Protein digestion was performed by incubating in trypsin (1 µg trypsin/50 µg protein; Thermo Fisher Scientific, Waltham, MA) for 3 h ...
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bioRxiv - Microbiology 2022Quote: ... reduced and alkylated followed by overnight trypsin digestion and peptide extraction following manufacturer’s instructions (In-Gel Tryptic Digestion kit, ThermoFisher Scientific). Extracted peptides were vacuum dried and redissolved in 2% acetonitrile in 0.1% formic acid in water.
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bioRxiv - Microbiology 2023Quote: ... Rigorous DNA digestion was done with the TURBO DNase kit (Invitrogen), and eukaryotic mRNA was removed by binding and discarding the eukaryotic mRNA polyA region to oligo d(T)25 magnetic beads (England Biolabs) ...
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bioRxiv - Immunology 2021Quote: In-solution digestion was performed according to the manufacturer’s instructions using ‘in-solution tryptic digestion and guanidination kit’ (#89895, Thermo Fisher Scientific, USA). The protocol can be summarized as follow ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... followed by chloroform-methanol protein precipitation to obtain pure protein pellets that were subsequently resuspended in digestion buffer (100 mM TEAB with 1% (v/v) SDC) and quantified by the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, United States). From each sample ...
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bioRxiv - Cell Biology 2020Quote: ... followed by DNase digestion (DNA-free DNA Removal Kit, Ambion, cat# AM1906). Alternatively ...
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bioRxiv - Immunology 2019Quote: ... RNA was then subjected to DNAse digestion (TURBO DNA-free Kit, Invitrogen) then purified again using Zymo RNA Clean and Concentrator and finally resuspended in DPEC-treated water.
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bioRxiv - Plant Biology 2020Quote: ... After digestion with TURBO DNA-free™ Kit (Thermo Fisher Scientific AM1907), cDNA was synthesized from 1 ug of RNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific 4368814) ...
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bioRxiv - Microbiology 2021Quote: ... followed by double DNAse digestion with the Turbo DNA-free Kit (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... followed by DNase digestion (DNA-free DNA Removal Kit, Ambion, cat# AM1906). Libraries were generated with the QuantSeq 3’ mRNA-Seq Kit REV for Illumina (Lexogen ...
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bioRxiv - Microbiology 2022Quote: ... RNA was treated by digestion with the TURBO DNA-free Kit (Ambion) to get rid of residual DNA contamination ...
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bioRxiv - Evolutionary Biology 2024Quote: ... This was followed by DNase digestions with Turbo DNA-free kit (Invitrogen) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by DNase digestion using the DNase I Amplification Grade Kit (Invitrogen) and a clean-up using RNeasy Min Elute columns (Qiagen) ...
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bioRxiv - Biochemistry 2022Quote: All proteolytic digestions of proteins were analyzed using an EASY-nLC 1000 system (Thermo Fisher Scientific) interfaced with an HF Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Robotic purification and digestion of proteins into peptides was performed on the Kingfisher Flex (ThermoFisher Scientific) using trypsin and the R2-P1 method [48] ...
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bioRxiv - Systems Biology 2023Quote: ... the RNA underwent protein digestion by adding 1 mg of Proteinase K (PK, Thermo Fisher Scientific) to 25 mg of purified RNA and incubating it at 37 ℃ for 45 minutes ...
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bioRxiv - Genetics 2021Quote: ... followed by DNAse digestion step using the TURBO DNA-freeTM kit (Ambion, AM1907). RNA concentration and quality were analyzed using an Agilent Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... eCC6.30.2 Fabs were purified by digesting IgG using Fab digestion kit (ThermoFisher, 44985) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... specimens underwent DNAse digestion using components of the TURBO DNA-free kit (Invitrogen) for the removal of any trace genomic DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were subjected to DNase digestion using the Turbo DNA-free kit (Invitrogen). RNA was reverse-transcribed using 2.5 μM oligo(dT) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... including on-column DNase digestion and subsequent TURBO DNA-freeTM Kit (AM1907 Ambion) treatment ...
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bioRxiv - Immunology 2020Quote: ... papain digestion was performed using the Pierce Fab preparation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Antibody digestion was performed using a Fab preparation kit (Thermo Fisher Scientific, USA) and the resulting Fab fragments were analyzed using SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... Two rounds of DNase digestion using TURBO DNA-free kit (Thermo Fisher Scientific) according to manufacturer’s instructions were performed to safely remove all remaining DNA ...
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bioRxiv - Genomics 2022Quote: ... DNA digestion was performed using the DNA-free DNA Removal Kit (ThermoFisher AM1906). Sequencing libraries of mRNA were prepared from 1 g total RNA using the NEBNext Ultra un-stranded preparation kit (E7775S,E7490S) ...
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bioRxiv - Genomics 2022Quote: ... followed by digestion of genomic DNA with the Turbo DNA-free kit (Ambion). The quality of extracted RNA was assessed with an Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Following digestions samples were labelled with TMT10plex Isobaric Label Reagents kit (Thermo Fisher) as previously described57 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were processed using the In-Gel Tryptic Digestion Kit (Thermo Scientific, Inc.) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... followed by a DNAse digestion step using the TURBO DNA-freeTM kit (Ambion, AM1907). Total RNA (1 μg ...
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bioRxiv - Neuroscience 2019Quote: ... An additional gDNA digestion was conducted using the TURBO DNA-free kit (Invitrogen #AM1907) (larval head samples ...
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bioRxiv - Immunology 2019Quote: ... a DNA digestion step was performed using an RNAqueous Mini Kit (Ambion, Massachusetts/USA). RNA purity and quantity were evaluated by spectrophotometry in a Nanodrop (Thermo Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... After DNase digestion of total RNA using the TURBO DNA-free™ Kit (Ambion), 500 ng were used in a reverse transcription reaction with Random Primers (Promega ...
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bioRxiv - Genomics 2021Quote: ... column extraction kit and completed on-column DNA digestion with PureLink DNase (Invitrogen #12185010). We quantified and assessed the quality of the RNA with an Agilent 2200 Tapestation instrument and then either froze down pure RNA for later RNA-sequencing library preparation or used ReadyScript cDNA Synthesis Mix (Sigma #RDRT-100RXN ...
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bioRxiv - Molecular Biology 2022Quote: ... After DNase digestion of total RNA using the TURBO DNA-free™ Kit (Ambion), 1 μg was used in a reverse transcription reaction with Random Primers (Promega ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to DNaseI digestion using the TURBO DNA-free kit (Thermo Fisher). RNA samples were then quantified by NanoDrop spectrophotometer and used for first strand cDNA synthesis performed with SuperScriptIII (Thermo Fisher ...