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Citations for Thermo Fisher :
1 - 50 of 7715 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... porcine AVICs or mouse heart valve tissue by using Trizol (Invitrogen) and purified by using RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... Porcine parvovirus [PPV] (VetMAX™ Porcine Parvovirus Kit, Thermo Fisher Scientific, MA, USA), PEDV ...
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bioRxiv - Microbiology 2023Quote: ... and testis tissue lysates were prepared in tissue extraction reagent (Thermo Fisher Sci Cat. # FNN0071) with 1X protease and phosphatase inhibitor (Thermo Fisher Sci Cat ...
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bioRxiv - Microbiology 2023Quote: ... and protein tissue lysates were prepared tissue protein extraction reagent (Thermo Fisher Sci. Cat. # FNN0071). SARS-CoV-2 titers in the normalized tissue homogenates were measured by plaque assay using Vero E6 cells as described previously (21) ...
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bioRxiv - Physiology 2022Quote: Cell and tissue lysates were prepared in Protein Extraction Reagent (ThermoFisher) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Physiology 2024Quote: ... Tissue lysates were prepared using Pierce RIPA buffer (89900, Thermo Scientific) supplemented with HaltTM phosphatase inhibitor cocktail (1861277 ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue lysates were homogenized with a Tissue Grinder (10 strokes, 02-911-529, Thermo Fisher Scientific) and centrifuged at 800□X□g for 5□min ...
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bioRxiv - Microbiology 2020Quote: ... Porcine serum (PS) (cat. 26250084, Gibco), Fetal Bovine Serum (FBS ...
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bioRxiv - Pathology 2021Quote: ... blocked with porcine serum (Thermofisher Scientific), and stained with a mixture of FITC-conjugated mouse-IgG2b anti-porcine CD4 antibody (clone MIL17) ...
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bioRxiv - Microbiology 2023Quote: ... containing 10% porcine serum (Gibco, USA). Porcine bone marrow cells were harvested from the hip bone and femur of 8-week-old pigs ...
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bioRxiv - Immunology 2020Quote: Cell and tissue lysates were prepared in Protein Extraction Reagent (Thermo Fisher) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... whole tissue lysates were prepared in cell extraction buffer (Thermo Fisher Scientific) supplemented with protease inhibitor cocktail P8340 ...
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bioRxiv - Genetics 2020Quote: Protein lysates were obtained from either pestle-homogenized tissues or cell lysates in M-PER lysis buffer (ThermoFisher; Cat# 78501) supplemented with 1x Complete Mini EDTA-Free protease inhibitor cocktail tablets (Roche Applied Sciences ...
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bioRxiv - Immunology 2019Quote: Mouse islets or neonatal porcine were isolated and placed into 12-well non-tissue culture-treated plates (150-200 islets/well; Fisher Scientific). Following an overnight culture cells were treated with 200 U/ml recombinant human TNF or of each IL1β ...
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bioRxiv - Developmental Biology 2020Quote: Tissue and whole cell lysates were prepared in Protein Extraction Reagent (Thermo Fisher) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue lysate in TRIzol was then mixed with UltraPureTM phenol:chloroform:isoamyl alcohol (ThermoFisher, #15593049) in a 5:1 ratio ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted to analyze the gene expression in each of the porcine organs (liver, lung, spleen, heart, kidney, testis, and ovary) and cultured porcine fibroblast cells using Trizol reagent (Invitrogen). Complementary DNAs (cDNAs ...
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bioRxiv - Microbiology 2019Quote: ... Tissue lysates were normalized for protein content with Pierce 660nm Protein Assay (Thermo Scientific), boiled in NuPAGE LDS sample buffer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Tissue lysates were cleared by centrifugation and quantified by BCA protein assay (Thermo Fisher) for western blot analysis.
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bioRxiv - Microbiology 2022Quote: Lung tissue was homogenized and lysates were labeled with NHS-activated AF555 (Thermo Fisher) as described by Pilobello et al [62] ...
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bioRxiv - Cell Biology 2021Quote: Tissue extracts and whole-cell lysates were prepared in radioimmunoprecipitation assay buffer (Thermo Scientific) supplemented with 1 mM PMSF ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysate was transferred into a 96 well opaque tissue culture plate (Thermo Fisher). For adherent cell lines ...
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bioRxiv - Physiology 2023Quote: ... RyR2 was immunoprecipitated from tissue lysate using anti-RyR2 antibody (Invitrogen Cat# MA3916, 5μL) in 0.5 mL RIPA buffer overnight at 4 °C as previously described 68 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor lysates were prepared by mincing tissue in RIPA buffer (Thermo Fisher Scientific, 89901) supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: 10 grams of porcine skin gelatin (Thermo Fisher) were dissolved in 240 mL of phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: Tissue lysates were prepared from cardiac ventricles excised from the heart using T-PERTM Tissue Protein Extraction Reagent (ThermoFisher Scientific, Tokyo, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Protein lysates were obtained by homogenizing tissues in M-PER lysis buffer (ThermoFisher; Cat# 78501) with 1x Complete Mini EDTA-free protease inhibitor cocktail tablets (Roche Applied Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... 200 uL of tissue lysate was used for KingFisher Flex (Thermo Fisher Scientific, Waltham, USA) RNA extraction according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Equal amounts of tissue lysates were pre-cleared using the Control Agarose Resin (Thermo Fisher). The antibody-antigen complexes were incubated overnight at 4°C and eluted following Pierce Co-Immunoprecipitation (Co-IP ...
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bioRxiv - Molecular Biology 2021Quote: ... Porcine oocytes were cultured in M-199 MEDIUM (Gibco) supplemented with 1 mM dbcAMP ...
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bioRxiv - Neuroscience 2019Quote: Tissue lysate were run on NuPAGE Novex 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific). Western blotting were performed as described (Saijilafu et al. ...
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bioRxiv - Molecular Biology 2020Quote: Mitochondrial isolates from heart tissue lysates were prepared according to the NativePAGE Sample prep kit (ThermoFisher) (8g/g ...
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bioRxiv - Developmental Biology 2023Quote: ... tissue lysates were divided in half by volume and added to TRIzol reagent (Thermo Fisher Scientific). Chloroform was added to achieve phase separation ...
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bioRxiv - Cancer Biology 2024Quote: Tissues and cell protein lysates were prepared with RIPA buffer (89900, Thermo Fisher Scientific, Waltham, MA), which includes a protease inhibitor cocktail (05892791001 ...
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bioRxiv - Immunology 2021Quote: ... Porcine Gene 1.1 ST Arrays (Affymetrix, Santa Clara, CA, USA) were hybridized to the biotinylated ss-cDNA targets ...
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bioRxiv - Developmental Biology 2023Quote: ... or siSOX9 for porcine (Life Technologies, Inc; Silencer Select 555768) using Lipofectamine RNAiMax Transfection Reagent (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... Tissues were lysed by pipetting and these lysates were incubated with 15 U of TURBO DNase (Invitrogen) for 10 min on ice ...
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bioRxiv - Neuroscience 2021Quote: The protein concentration of frozen tissue lysates was determined using the BCA assay (Pierce, ThermoFisher catalog #23225) and diluted with 4x LDS sample buffer (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Protein concentration of tissue lysate samples was determined by Pierce® 660 nm Protein Assay (Thermo Scientific). 40-50µg total protein of each tissue lysate sample and 20ug of cell lysate sample was added to 3.75µL4x NuPAGE buffer (106mM Tris HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared from cultured cells with modified Tissue Protein Extraction Reagent (TPER) (Thermo Fisher Scientific) and a cocktail of protease and phosphatase inhibitors (Roche ...
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bioRxiv - Immunology 2021Quote: ... connected by a porcine teschovirus-1 2A element (“P2A”; Life technologies), recognize the Msln406-414 epitope presented in H2-Db ...
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bioRxiv - Biochemistry 2022Quote: Constructs of full-length porcine p110γ were cloned into pVL1393 (Invitrogen). The plasmid for EE-tagged mouse p84 was a gift from Len Stephens (The Babraham Institute ...
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bioRxiv - Bioengineering 2019Quote: ... β-galactosidase enzymatic activity in tissue lysates was measured using the Galacto-Star System (Applied BioSystems; Bedford, MA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher) respectively supplemented with proteinase and phosphatase inhibitors (Sigma ...
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bioRxiv - Cell Biology 2023Quote: Total proteins were harvested from tissue and cell lines lysates using M-PER protein extraction reagent (Thermo Fisher) and supplemented with proteinase and phosphatase inhibitors (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations of the cell and tissue lysates were determined using BCA protein assay kit (Pierce, Thermo Fisher Scientific). Lysates were subjected to SDS-PAGE and proteins were transferred onto a nitrocellulose membrane (Biorad) ...
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bioRxiv - Neuroscience 2022Quote: Tissue and cell lysates were prepared in Cell Lysis Buffer containing HALT protease and phosphatase-inhibitor cocktail (ThermoFisher Scientific). Samples were run on 15 well Mini-Protean TGX 4%– 15% ...
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bioRxiv - Neuroscience 2023Quote: Whole cell protein lysates were isolated from entire hippocampal tissues using RIPA buffer (Thermo Fisher Scientific, Catalogue No. R0278) in the presence of protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... human stool extracts and mouse colonic tissue lysates were resolved on Bolt 4-12% Bis-Tris Plus Gels (Invitrogen), transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... protein lysates were prepared from cultured cells with modified Tissue Protein Extraction Reagent (TPER) (Life Technologies Corporation, Carlsbad, CA) and a cocktail of protease and phosphatase inhibitors (Roche ...