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Citations for Thermo Fisher :
1 - 50 of 4797 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Primary placental fibroblasts were obtained by culturing placental digests in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Immunology 2021Quote: Sorted HSC were also cultured with blocking CD150 peptide (FCKQLKLYEQVSPPE, Auspep, 100 μg/ml) or control peptide (DLSKGSYPDHLEDGY, Auspep,100 μg/ml) (Thermo Scientific).
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bioRxiv - Developmental Biology 2022Quote: First-trimester placental explants were submerged in DMEM/F12(Gibco) media and divided into smaller pieces under the dissection microscope in sterile conditions ...
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bioRxiv - Developmental Biology 2019Quote: ... Total RNA was prepared from placental villous samples thawed in RNAIce Later (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: Placental tissues were lysed by homogenization in cell disruption buffer (PARIS kit, Ambion) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Placental and hepatic tissues were homogenized in 900μl of TRIzol reagent (15596018; Invitrogen) using glass homogenizing beads (10064583 ...
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bioRxiv - Immunology 2021Quote: Placental tissue was placed in RIPA Lysis and Extraction Buffer (ThermoFisher Scientific #89901) containing Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific #78443 ...
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bioRxiv - Cell Biology 2024Quote: ... The placental sections were treated with streptavidin HRP tertiary reagent (Thermo Fisher Scientific) for 1 h and detected using a DAB+ staining kit (Dako) ...
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bioRxiv - Immunology 2022Quote: ... 1×105 placental neutrophils were co-cultured with 1×105 splenic monocytes in DMEM (Gibco) growth media containing 10% FCS and 50 IU/mL penicillin ...
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bioRxiv - Physiology 2023Quote: Placental RNA was extracted using TRIzol following the manufacturer’s protocol (TRIzol Reagent; Life Technologies, USA). The concentration of RNA was assessed using a NanoPhotometer (Implen ...
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bioRxiv - Developmental Biology 2021Quote: ... After blocking with blocking buffers (Thermo Scientific) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from the chorionic villi samples or placental villus organoids using Trizol (Invitrogen, #15596026) according to manufacturer’s instruction ...
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bioRxiv - Physiology 2021Quote: Total placental RNA was extracted using TRIzol reagent according to the manufacturer’s instructions (Life Technologies, CA, USA). The total RNA concentration was assessed using a nanophotometer (Implen ...
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bioRxiv - Pathology 2021Quote: ... full thickness placental biopsies (1cm x 3cm) were collected and fixed in 10% neutral buffered formalin (ThermoFisher) for 72 hours ...
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bioRxiv - Physiology 2023Quote: Placental protein extraction was performed on frozen tissue homogenized using commercial RIPA lysis buffer (Thermo Scientific, US) supplemented with phosphatase inhibitors (5 mM Na4P2O7 ...
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bioRxiv - Physiology 2020Quote: ... Uterine and placental RNA purities (A260/A280 ratios) were evaluated with a NanoDrop 1000 spectrometer (Thermo Fisher Scientific). Only samples presenting a ratio greater than 1.8 were kept for further analyses ...
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bioRxiv - Immunology 2021Quote: RNA was isolated from frozen placental decidual biopsies (50-75 μg) using the RNAqueous™ kit (#AM1912, Invitrogen). RNA quantity and purity was assessed using a Nanodrop™ spectrophotometer (ThermoFisher) ...
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bioRxiv - Genomics 2022Quote: ... total placental RNA was extracted from approximately 5 mg of tissue using the “mirVana miRNA Isolation Kit” (Ambion) which efficiently isolates all RNAs longer than 10 nucleotides in length ...
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bioRxiv - Genomics 2022Quote: The POP study placental biopsies were collected within 30 minutes of delivery and flash frozen in RNAlater (ThermoFisher). For each biopsy ...
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bioRxiv - Cell Biology 2022Quote: ... and then blocking in Superblock Blocking Buffer (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed using BlockAid Blocking Solution (Thermo Fisher Scientific). Primary antibody incubation against clathrin heavy chain (ab21679 from Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... Blocking Buffer (Invitrogen Cat# 00-4952-54 ...
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bioRxiv - Developmental Biology 2023Quote: Placental tissues (6th-12th week of gestation) were washed with Mg2+/Ca2+-free Hanks balanced salt solution (HBSS, Gibco) and villus-trees were mechanically dissected ...
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bioRxiv - Molecular Biology 2023Quote: Total mRNA from hemisected placental tissues and embryonic brain tissues were extracted following homogenization in Trizol Reagent (Thermo Fisher) with subsequent clean-up using RNeasy Microcolumns (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and blocking in SuperBlock Blocking Buffer (Thermo Fisher Scientific, Waltham, MA). Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175 ...
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bioRxiv - Cancer Biology 2019Quote: ... After blocking with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific), the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... before blocking in SuperBlock™ blocking buffer (Thermo Fisher Scientific, 37515). Primary antibodies (MAP2 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... followed with endo-biotin blocking using a blocking kit (Invitrogen, #E21390) according to the manufacture instructions [118] ...
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bioRxiv - Microbiology 2023Quote: ... blocking was performed overnight using SEA BLOCK Blocking buffer (ThermoFisher, 37527) at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations of eluted peptides were quantified using the Pierce Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... first trimester placental villous tissues were cut and digested in PBS supplemented with 0.25% Trypsin (Gibco, Grand Island, NY, USA) for 10 min at 37°C with gentle agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA from placental and mammary tissues was isolated with the TRIZOL RNA Extraction Kit (Invitrogen, Carlsbad, CA, United States). The integrity of the extracted RNA was meticulously assessed through several quality control measures ...
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bioRxiv - Biophysics 2024Quote: ... prepolymer solutions of the 3900 kPa native placental stiffness containing 0.5% volume of 0.5 μm diameter carboxylate-modified fluorescent beads in PBS (FluoSpheres, Invitrogen, Catalog: F8812) were prepared as previously described34 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... proteins were extracted from homogenized mouse placental tissues or cell samples using RIPA lysis buffer (89900, Thermo Fisher Scientific, USA) containing protease and phosphatase inhibitors (A32961 ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific) and 5 µg of total peptides were labeled using TMTpro16-plex reagents (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were desalted with peptide desalting spin columns (Thermo Scientific) and dried via vacuum centrifugation ...
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bioRxiv - Cancer Biology 2022Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Biochemistry 2023Quote: Peptide samples were assayed for peptide concentration with Pierce™ Quantitative Colorimetric Peptide Assay (Thermofisher Scientific, Brisbane, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Labelled peptides were quantified (Pierce Colorimetric Peptide Assay Kit, Thermo Scientific) and mixed 1:1:1 based on peptide amount.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were quantified using a colorimetric peptide assay (Thermo Fisher Scientifc). Peptides (1 µg ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using colourimetric peptide assay (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using a colorimetric peptide assay (ThermoFisher Scientific). All samples were reconstituted in loading buffer (5% CH3CN ...
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bioRxiv - Cell Biology 2019Quote: ... or pCMV-Gag-Pol-RcRE(pHR5338) and 100ng of a plasmid expressing secreted placental alkaline phosphatase (SEAP) (pHR1831) using Turbofect™ transfection reagent (ThermoFisher). Cell supernatants were collected 72 hours post-transfection ...
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bioRxiv - Pathology 2021Quote: RNA was extracted from fresh frozen villous placental tissue biopsies with using RNA was isolated using an RNAqueous™ kit (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: Arteries were dissected from placental tissue and rinsed with ice-cold sterile Hanks’ Balanced Salt Solution (HBSS; Gibco, Waltham, MA, USA). Arteries were cut open lengthwise ...
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bioRxiv - Cell Biology 2023Quote: Human placental–epithelial choriocarcinoma cells (JEG-3 HTB-36TM ATCC®) were cultured in Earle’s Minimum Essential Medium (EMEM) (Gibco™) containing 2-mM L-glutamine supplemented with 1% nonessential amino acids (Gibco™) ...
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bioRxiv - Biophysics 2021Quote: ... blocking of endogenous biotin was performed using a commercial blocking kit (Invitrogen, E21390). Cells were incubated in component A for 45 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... After blocking with 10% goat serum or Superblock blocking buffer (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Peptide digest assay standard (PierceTM Quantitative Colorimetric Peptide Assay, Thermo Scientific, USA) was as standard for quantitative analysis of peptides ...