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Citations for Thermo Fisher :
1 - 50 of 3664 citations for Phospholipase A2 Activating Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: Quantification of Phospholipase A2 (PLA2) activity was performed using the EnzChek Phospholipase A2 Assay Kit (Thermo Fisher). 50 μl of cell lysate/well ...
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bioRxiv - Molecular Biology 2023Quote: The potential phospholipase A2 (PLA2) activity of Dr20/22 was evaluated using the EnzChek™ Phospholipase A2 Assay Kit (Invitrogen). Fresh or frozen (stored at -80 °C ...
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bioRxiv - Molecular Biology 2024Quote: Phospholipase activity was measured using the EnzChek Phospholipase A2 Assay Kit (Invitrogen, cat. no. E10217) for 96-well plates ...
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bioRxiv - Microbiology 2023Quote: ... the EnzChek Phospholipase A2 activity kit was used (Thermo Fisher), according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we used the EnzChek™ Phospholipase A2 Assay Kit (#E10217, Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: PLA2 activity was measured using an EnzCheck Phospholipase A2 assay kit (E10217, ThermoFisher Scientific), as previously described (21) ...
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bioRxiv - Neuroscience 2023Quote: Phospholipase A1 and A2 activity were determined using commercial assays (Cat# E10217 and E10219, Thermofisher). The protocol for determining activity was based on the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Phospholipase activity was monitored using the EnzChek Phospholipase A1 assay kit (ThermoFisher) as described previously (36) ...
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bioRxiv - Microbiology 2023Quote: Phospholipase A1 activity was assayed using the EnzChek™ Phospholipase A1 Assay Kit (Thermofisher), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: The in vitro phospholipase activity assay was performed using the Amplex Red Phospholipase D Assay Kit (A12219, Invitrogen) and 1,2-dioctanoyl-sn-glycero-3-phosphocholine (8:0 PC ...
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bioRxiv - Cell Biology 2024Quote: ... anti-annexin A2 (Invitrogen, mouse clone Z014 polyclonal for Western Blot and imaging) ...
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bioRxiv - Cell Biology 2020Quote: For phosphatidylinositol-specific phospholipase C (PI-PLC; Thermo Fisher Scientific) treatment of endogenous GPI-APs ...
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bioRxiv - Immunology 2024Quote: ... anti-CD3/CD28 T-cell activating DynaBeads (Thermo scientific) were magnetically washed in 1mL DPBS and added to T-cells at a 1:1 ratio ...
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bioRxiv - Microbiology 2022Quote: PLA1 activity was monitored using EnzCheck Phospholipase Assay Kits (Invitrogen, Life Technologies) according to the manufacturer’s instructions and as previously described in 44 ...
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bioRxiv - Microbiology 2022Quote: PLA1 activity was monitored using EnzCheck Phospholipase Assay Kits (Invitrogen, Life Technologies) according to the manufacturer’s instructions and as previously described in 44 ...
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bioRxiv - Cancer Biology 2023Quote: ... was annealed from two components: trans-activating crRNA (A35507, Invitrogen) and crRNA targeting specific gene (A35509 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-cyclin A2 monoclonal antibody (Invitrogen MA1180), mouse anti-actin monoclonal antibody (Invitrogen MA1744) ...
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bioRxiv - Cancer Biology 2021Quote: ... HLA-A2 (141Pr, BB7.2, Thermo Fisher, MA1-80117)
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bioRxiv - Cancer Biology 2023Quote: ... Aliquots of transfected cells were retained for validation of HLA-A2 expression by staining with HLA-A2 monoclonal antibody (BB7.2, Thermo Scientific, 17-9876-42) and subsequent flow cytometry analysis.
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bioRxiv - Microbiology 2023Quote: ... cells were activated with with αCD3/αCD28 activating beads (ThermoFisher Scientific, Cat. #11132D) for 48h at a concentration of 1 bead/cell in the presence of 100 U/ml IL-2 (PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μl of 100 U/ml phosphatidylinositol-specific phospholipase C (PI-PLC, Life Technologies P-6466) was added to the treatment well ...
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bioRxiv - Cell Biology 2022Quote: Phosphatidylinositol-specific phospholipase C (PI-PLC) treatment was carried out by adding 1 mL L15 (Gibco, 21083027) + 10% heat-inactivated FBS (hiFBS ...
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bioRxiv - Bioengineering 2023Quote: K562 A2/PPI cells were labeled with CellTrace Violet (CTV, Invitrogen) and seeded in a 48 well plate at 30,000 cells/well in 300 μl cRPMI ...
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bioRxiv - Bioengineering 2024Quote: ... Type A2 biological safety cabinet (Thermo Fisher Scientific, Waltham, MA, USA). The fish ventral area was disinfected three times by rubbing with 70% ethanol before incision ...
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bioRxiv - Plant Biology 2022Quote: Anti-WPRA1/A2 and anti-RFP (Rabbit polyclonal, Invitrogen R10367, Lot 2127438) were coupled with Dynabeads prepared according to the Dynabeads kit instructions (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then treated with human serum containing phospholipase D (Australian Red Cross Lifeblood) or 0.25% (w/v) Albumax II (Gibco) and incubated for 3 hours at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... DMS53 (A2) cells were grown in Waymouth’s medium (cat no. 11220-035, Gibco) supplemented with 10% FBS ...
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bioRxiv - Biophysics 2023Quote: N130 and N130+A2 were labeled with Alexa-488 (ThermoFisher, Waltham, MA, USA) at Cys104 by incubating a molar excess of Alexa-488 maleimide with freshly reduced N130 proteins overnight at 4 °C with oscillation ...
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bioRxiv - Immunology 2020Quote: ... or Raji and Jurkat cells as a positive control were incubated with 1 U/ml of phosphatidylinositol-specific phospholipase C (PI-PLC; Invitrogen) at 4°C for 30 minutes in cold PBS ...
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bioRxiv - Molecular Biology 2021Quote: Cells (1×108) stably expressing HFGF-CD59 were treated with 0.05 units/ml of phosphatidylinositol-specific phospholipase C (PI-PLC) (ThermoFisher, P6466) in 10 ml of PI-PLC buffer (Opti-MEM containing 10mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from transduced A2 and 11.1 cells using Trizol (Thermo Fisher) on day 14 after infection using Total RNA Zol out kit (A&A Biotechnology) ...
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bioRxiv - Cell Biology 2021Quote: Purified recombinant GST-tagged CDK1-cyclin A2 and His6-tagged CDK1-cyclin B1 (Invitrogen) were stored in 150 mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... and HLA-A2+ (PE, APC or BV421, clone BB7.2, Biolegend, BD Biosciences or Invitrogen). Cells were stained intracellularly for FOXP3 ...
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bioRxiv - Plant Biology 2021Quote: ... The ATG8-PE (lipidation) band was confirmed by incubating protein samples at 37°C for 1 hour with Streptomyces chromofuscus phospholipase D (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... the stable thromboxane (Tx) A2 mimetic U46619 (10 μM; Enzo) or ionomycin (10 μM; Invitrogen) and subsequent recording for 2 min ...
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bioRxiv - Cancer Biology 2022Quote: ... alongside aforementioned activating proteins and CellEvent™ Caspase-3/7 Green Detection Reagent (1:5000, ThermoFisher Scientific, Cat#C10423). Cells were imaged using the IncuCyte ZOOM System (Essen Bioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... The RRtoGG αEβ7 ectodomain was diluted 1:10 in non-activating buffer and incubated with digested LF61 Fab (Invitrogen) at a 1:4 molar ratio for 45 minutes at room temperature with end-over-end mixing ...
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bioRxiv - Cell Biology 2020Quote: ... and hTERT-RPE-1 Cyclin A2-eYFP54 were cultured with DMEM/F-12 + GlutaMax (Life Technologies), and BJ primary human foreskin fibroblasts (a kind gift from Bennie Lemmens ...
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bioRxiv - Bioengineering 2021Quote: ... 105 PBMCs derived from a healthy human donor were stimulated with T cell activating beads (anti-CD3/CD28 Dynabeads, ThermoFisher) at a 1:1 PBMC:bead ratio in each well containing MSCs ...
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bioRxiv - Immunology 2020Quote: ... T cells are enumerated using Trypan Blue exclusion and co-incubated with CD3/CD28 Human T cell Activating Dynabeads (ThermoFisher) at a 3:1 bead:T cell ratio in a total volume of 1 ml TCM supplemented with 50 units/ml recombinant IL-2 in a 48-well plate ...
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations of eluted peptides were quantified using the Pierce Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific) and 5 µg of total peptides were labeled using TMTpro16-plex reagents (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were desalted with peptide desalting spin columns (Thermo Scientific) and dried via vacuum centrifugation ...
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bioRxiv - Biochemistry 2023Quote: Peptide samples were assayed for peptide concentration with Pierce™ Quantitative Colorimetric Peptide Assay (Thermofisher Scientific, Brisbane, Australia), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... elegans genes was cloned into the pDEST22 vector containing the GAL4 activating domain (AD) using the CloneMinerTM cDNA library construction kit (ThermoFisher, A11180). A forward Y2H library screen was performed using the ProQuest Two-Hybrid System (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... The tubes were quickly removed and taken to a Biological Safety Cabinet (Thermo Fisher, 1300 Series A2) that was pre-cleaned with RNaseZap ...
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bioRxiv - Molecular Biology 2023Quote: ... were transfected into A1/A2 dKO Raw 264.7 cells using a Neon Transfection System (Thermo Fisher Scientific) with the following parameters ...
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bioRxiv - Microbiology 2022Quote: ... Labelled peptides were quantified (Pierce Colorimetric Peptide Assay Kit, Thermo Scientific) and mixed 1:1:1 based on peptide amount.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were quantified using a colorimetric peptide assay (Thermo Fisher Scientifc). Peptides (1 µg ...
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bioRxiv - Cell Biology 2024Quote: Peptide concentration was determined using colourimetric peptide assay (Thermo Fisher Scientific). Briefly ...